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41 条记录 (耗时 0.053 秒)

    Monoclonal antibodies against melanoma
    23.
    发明授权
    Monoclonal antibodies against melanoma 失效
    针对黑色素瘤的单克隆抗体

    公开(公告)号：US5610280A

    公开(公告)日：1997-03-11

    申请号：US214020

    申请日：1994-03-15

    申请人： Michael Brandt ,  Josef Endl ,  Herbert Jungfer ,  Winfried Albert

    发明人： Michael Brandt ,  Josef Endl ,  Herbert Jungfer ,  Winfried Albert

    IPC分类号： A61K39/395 ,  A61P35/00 ,  C07K16/00 ,  C07K16/30 ,  C07K19/00 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/574 ,  G01N33/577 ,  A61K51/10 ,  C12N5/12 ,  G01N33/53

    CPC分类号： C07K16/3053

    摘要： The present invention provides a human monoclonal antibody against melanoma, characterized in that it binds to the gangliosides GM3 and GD3 but essentially does not bind to the gangliosides GM1, GM2, GD1a, GD1b and GD2, the binding of the antibody to the gangliosides having been determined by immune staining after thin layer chromatographic separation of the gangliosides. The present invention also provides a process for the production of human monoclonal antibodies directed against melanoma, wherein, without previous immunization, B-lymphocytes are isolated from a healthy person, the isolated B-lymphocytes are immortalized, antibodies from the immortalized B-lymphocytes are screened by immune-histochemical analysis for binding against melanoma and/or melanoma metastases, the positively reacting B-lymphocytes are selected, cultured and monoclonal antibodies obtained therefrom.

    摘要翻译： 本发明提供了针对黑素瘤的人单克隆抗体，其特征在于其结合神经节苷脂GM3和GD3，但基本上不结合神经节苷脂GM1，GM2，GD1a，GD1b和GD2，抗体与神经节苷脂的结合 通过神经节苷脂薄层色谱分离后的免疫染色测定。 本发明还提供了一种制备针对黑素瘤的人单克隆抗体的方法，其中，在没有先前免疫的情况下，从健康人分离B淋巴细胞，分离的B淋巴细胞被永生化，来自永生化B淋巴细胞的抗体是 通过用于结合黑素瘤和/或黑素瘤转移的免疫组织化学分析进行筛选，选择阳性反应性B淋巴细胞，培养和从其获得的单克隆抗体。

    TRACKING REGULATOR SYSTEM AND METHOD FOR PROCESSING COLUMNS
    25.
    发明申请
    TRACKING REGULATOR SYSTEM AND METHOD FOR PROCESSING COLUMNS 审中-公开
    跟踪调节器系统和处理柱的方法

    公开(公告)号：US20110259831A1

    公开(公告)日：2011-10-27

    申请号：US12765498

    申请日：2010-04-22

    申请人： Michael Brandt ,  Mark Dunn ,  Louis Bellafiore

    发明人： Michael Brandt ,  Mark Dunn ,  Louis Bellafiore

    IPC分类号： G05D16/00 ,  B01D17/12

    CPC分类号： G01N30/6021 ,  G01N30/56

    摘要： A processing column system includes an inlet, an outlet, a piston head and a piston pressure chamber for providing a controlled bed pressure to the piston head and thus to a substrate and/or liquid bed positioned within the column. A tracking regulator is in fluid communication with a source of pressurized hydraulic fluid and the piston pressure chamber as well as the inlet to the column. The tracking regulator receives a pressure of a process fluid flow into the processing column. The tracking regulator has a hydraulic fluid drain port in fluid communication with the source of pressurized hydraulic fluid. The tracking regulator directs hydraulic fluid from the piston pressure chamber to the source of pressurized hydraulic fluid when the tracking regulator detects a drop in the pressure of the process fluid flow. The source of pressurized hydraulic fluid provides to the tracking regulator a pressure equal to a set bed pressure of the system plus a maximum anticipated process flow pressure. The tracking regulator directs additional hydraulic fluid from the source of pressurized hydraulic fluid to the piston pressure chamber of the processing column so that a pressure therein is greater than the set bed pressure when the tracking regulator detects an increase in the pressure of the process fluid flow.

    摘要翻译： 处理塔系统包括入口，出口，活塞头和活塞压力室，用于向活塞头提供受控床压力，从而提供位于柱内的基底和/或液床。 跟踪调节器与加压液压流体源和活塞压力室以及塔的入口流体连通。 跟踪调节器接收进入处理柱的工艺流体流的压力。 跟踪调节器具有与加压液压流体源流体连通的液压流体排放口。 当跟踪调节器检测到过程流体流的压力下降时，跟踪调节器将液压流体从活塞压力室引导到加压液压流体源。 加压液压流体的源向跟踪调节器提供等于系统的设定床压力加上最大预期过程流压力的压力。 跟踪调节器将额外的液压流体从加压液压流体源引导到处理塔的活塞压力室，使得当跟踪调节器检测到过程流体流的压力增加时，其中的压力大于设定的床压力 。

    [[2-(Amino-3,4-dioxo-1-cyclobuten-1-yl)amino]alkyl]-acid derivatives for the treatment of pain
    28.
    发明申请
    [[2-(Amino-3,4-dioxo-1-cyclobuten-1-yl)amino]alkyl]-acid derivatives for the treatment of pain 失效
    [[2-（氨基-3,4-二氧代-1-环丁烯-1-基）氨基]烷基] - 酸衍生物，用于治疗疼痛

    公开(公告)号：US20060205696A1

    公开(公告)日：2006-09-14

    申请号：US11433737

    申请日：2006-05-12

    申请人： Michael Brandt ,  Margaret Zaleska ,  John Moyer

    发明人： Michael Brandt ,  Margaret Zaleska ,  John Moyer

    IPC分类号： A61K31/663 ,  A61K31/4245 ,  A61K31/41 ,  A61K31/195 ,  A61K31/215

    CPC分类号： C07D243/10 ,  A61K31/122 ,  A61K31/198 ,  A61K31/4245 ,  A61K31/551 ,  A61K31/662 ,  A61K45/06

    摘要： This invention provides a method of treating pain in a mammal that includes administering to a mammal in need of such treatment a pain treating effective amount of a compound of the formula (I): where R1 is H, alkyl or phenylalkyl; R2 is H, alkyl, alkenyl or phenylalkyl; or R1 and R2 taken together as Z are —CH2C—H2—, —CH2C(R6)(R7)CH2— or —CH2C(R8)(R9)—C(R10)(R11)CH2—, where R6, R8 and R10 are, independently, H, alkyl or hydroxyl and R7, R9 and R11 are, independently, H or alkyl; A is alkylene or alkenylene; X is CO2R3, P(O)(OR4)(OR5), 3,5-dioxo-1,2,4-oxadiazolidin-2-yl or 5-tetrazolyl in which R3, R4 and R5 are, independently, H or alkyl, or a pharmaceutically acceptable salt thereof. The present invention also provides pharmaceutical compositions for treating pain containing a pain treating effective amount of the compound of formula (I).

    摘要翻译： 本发明提供一种治疗哺乳动物疼痛的方法，包括向需要这种治疗的哺乳动物施用疼痛治疗有效量的式（I）化合物：其中R 1是H， 烷基或苯基烷基; R 2是H，烷基，烯基或苯基烷基; 或R 1和R 2一起作为Z是-CH 2 CH 2 - ， - CH＆lt; SUB （R 6）（R 7）CH 2 - 或-CH 2 CH 2 C（R 8）（R 9） - （R 10） - （R 11） 其中R 6，R 8和R 10独立地为H，烷基或羟基，R 1，R 2， R 9，R 9和R 11独立地是H或烷基; A是亚烷基或亚烯基; X是CO 2 R 3，P（O）（OR 4）（OR 5），3， 5-二氧代-1,2,4-恶二唑烷-2-基或其中R 3，R 4和R 5的5-四唑基， 独立地是H或烷基，或其药学上可接受的盐。 本发明还提供用于治疗疼痛的药物组合物，其含有疼痛治疗有效量的式（I）化合物。

    Monoclonal antibodies of isotype IgG which bind specifically to glutamate decarboxylase, and uses thereof
    30.
    发明授权
    Monoclonal antibodies of isotype IgG which bind specifically to glutamate decarboxylase, and uses thereof 失效
    与谷氨酸脱羧酶特异性结合的同种型IgG的单克隆抗体及其用途

    公开(公告)号：US06214568B1

    公开(公告)日：2001-04-10

    申请号：US09124410

    申请日：1998-07-29

    申请人： Josef Endl ,  Michael Brandt ,  Herbert Jungfer ,  Winfried Albert ,  Rosemarie Kientsch-Engel ,  Werner Scherbaum ,  Wiltrud Richter ,  Thomas Eiermann

    发明人： Josef Endl ,  Michael Brandt ,  Herbert Jungfer ,  Winfried Albert ,  Rosemarie Kientsch-Engel ,  Werner Scherbaum ,  Wiltrud Richter ,  Thomas Eiermann

    IPC分类号： G01N33573

    CPC分类号： C07K16/40 ,  C07K14/47 ,  C07K16/18 ,  C07K16/4241 ,  C07K2317/21

    摘要： The invention concerns human monoclonal antibodies of the IgG isotype against human pancreatic islet cells which can be obtained by immortalizing human lymphocytes of prediabetics or diabetics, treating the culture supernatant of the immortalized cells with a conjugate of antibodies against human Fc &ggr; and a label, subsequently treating with human immunoglobulin, incubating with immobilized human pancreatic islet cells identifying an immortalized human cell culture which produces an antibody against pancreatic islet cells via determination of the label bound to the immobilized islet cells, isolating a human immortalized cell which produces this antibody, propagating this immortalized cell and isolating the monoclonal antibody produced by these cells. The invention also concerns a process for the isolation of an islet cell antigen to which such antibodies bind as well as a method for the determination of antibodies against an islet cell antigen of the pancreas.

    摘要翻译： 本发明涉及针对人胰岛细胞的IgG同种型的人单克隆抗体，其可以通过使老年糖尿病患者或糖尿病患者的人淋巴细胞永生化而获得，用抗Fcγ和标记的抗体缀合物处理永生化细胞的培养物上清液，随后 用人免疫球蛋白处理，与固定的人胰岛细胞孵育，鉴定永生化​​的人细胞培养物，其通过测定与固定化胰岛细胞结合的标记产生抗胰岛细胞的抗体，分离产生该抗体的人永生化细胞，传播该抗体 永生化细胞并分离由这些细胞产生的单克隆抗体。 本发明还涉及用于分离这种抗体结合的胰岛细胞抗原的方法以及用于测定抗胰岛细胞抗原的抗体的方法。