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74 条记录 (耗时 0.05 秒)

    NON-INVASIVE CARDIAC OUTPUT DETERMINATION
    22.
    发明申请
    NON-INVASIVE CARDIAC OUTPUT DETERMINATION 审中-公开

    公开(公告)号：US20170224230A1

    公开(公告)日：2017-08-10

    申请号：US15438503

    申请日：2017-02-21

    申请人： Joseph Fisher ,  Michael Klein ,  James Duffin

    发明人： Joseph Fisher ,  Michael Klein ,  James Duffin

    IPC分类号： A61B5/0205 ,  A61B5/00

    CPC分类号： A61B5/0205 ,  A61B5/026 ,  A61B5/029 ,  A61B5/0813 ,  A61B5/082 ,  A61B5/0836 ,  A61B5/7225 ,  A61B5/7278 ,  A61M16/0057 ,  A61M16/12

    摘要： A method of controlling a gas delivery apparatus including an apparatus controllable variable using an iterative algorithm to deliver a test gas (TG) for non-invasively determining a subject's pulmonary blood flow comprising iteratively generating and evaluating test values of a iterated variable based on an iterative algorithm in order output a test value of the iterated variable that meets a test criterion wherein iterative algorithm is characterized in that it defines a test mathematical relationship between the at least one apparatus controllable variable, the iterated variable and an end tidal concentration of test gas attained by setting the apparatus controllable variable, such that the iterative algorithm is determinative of whether iteration on the test value satisfies a test criterion or iteratively generates a progressively refined test value.

    Multiparameter FACS assays to detect alterations in exocytosis
    28.
    发明授权
    Multiparameter FACS assays to detect alterations in exocytosis 失效
    多参数FACS测定以检测胞吐的变化

    公开(公告)号：US06897031B1

    公开(公告)日：2005-05-24

    申请号：US09062330

    申请日：1998-04-17

    申请人： Joseph Fisher ,  James Lorens ,  Donald Payan ,  Alexander Rossi

    发明人： Joseph Fisher ,  James Lorens ,  Donald Payan ,  Alexander Rossi

    IPC分类号： G01N33/50 ,  G01N33/569 ,  G01N33/567

    CPC分类号： G01N33/5011 ,  G01N33/5008 ,  G01N33/5014 ,  G01N33/502 ,  G01N33/5047 ,  G01N33/56966 ,  G01N2333/43595 ,  G01N2333/4718

    摘要： Described is a method for screening for alterations in exocytosis of a population of cells. The cells are sorted by a FACS machine by assaying for alterations in at least three of the properties selected from the group consisting of light scattering, fluorescent dye uptake, fluorescent dye release, annexin granule binding, surface granule enzyme activity, and the quantity of granule specific proteins. Methods for screening for bioactive agents capable of modulating exocytosis in a cell are also described. The methods provide for reduced background and increased specificity without increasing the time or steps involved in assaying for exocytosis.

    摘要翻译： 描述了筛选细胞群的胞吐作用的方法。 细胞由FACS机通过测定至少三种选自光散射，荧光染料摄取，荧光染料释放，膜联蛋白颗粒结合，表面颗粒酶活性和颗粒数量的性质的变化进行分选 特异性蛋白质。 还描述了筛选能够调节细胞中胞吐作用的生物活性剂的方法。 所述方法提供降低的背景和增加的特异性，而不增加测定胞吐的时间或步骤。

    Exo1 and Exo2, exocytotic proteins
    29.
    发明授权
    Exo1 and Exo2, exocytotic proteins 有权
    Exo1和Exo2，胞吐蛋白

    公开(公告)号：US06623980B1

    公开(公告)日：2003-09-23

    申请号：US09255920

    申请日：1999-02-23

    申请人： Joseph Fisher ,  James Lorens ,  David Anderson ,  Ying Luo ,  Chao Bai (Betty) Huang ,  Mary Shen

    发明人： Joseph Fisher ,  James Lorens ,  David Anderson ,  Ying Luo ,  Chao Bai (Betty) Huang ,  Mary Shen

    IPC分类号： G01N33566

    CPC分类号： C07K14/4703

    摘要： The present invention is directed to novel exocytotic polypeptides, such as Exo1 and Exo2 polypeptides and related molecules, which have an inhibitory effect on exocytosis and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided by the present invention are method for identifying novel compositions which mediate exocytotic polypeptide bioactivity, and the use of such compositions in diagnosis and treatment of disease.

    摘要翻译： 本发明涉及新颖的胞吐多肽，例如Exo1和Exo2多肽及相关分子，其对胞吐作用具有抑制作用，以及编码这些多肽的核酸分子。 本文还提供了包含那些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明的多肽的嵌合多肽分子，与本发明的多肽结合的抗体以及本发明的多肽的制备方法 发明。 本发明还提供了鉴定介导胞吐多肽生物活性的新组合物的方法，以及这些组合物在疾病诊断和治疗中的应用。

    Non-invasive method and apparatus for measuring mixed venous carbon dioxide pressure (PvCO.sub.2) and other physiological variables
    30.
    发明授权
    Non-invasive method and apparatus for measuring mixed venous carbon dioxide pressure (PvCO.sub.2) and other physiological variables 失效
    用于测量混合静脉二氧化碳压力（PvCO2）和其他生理变量的非侵入性方法和装置

    公开(公告)号：US4947860A

    公开(公告)日：1990-08-14

    申请号：US912969

    申请日：1986-09-29

    申请人： Joseph Fisher

    发明人： Joseph Fisher

    IPC分类号： A61B5/083 ,  G01N33/49

    CPC分类号： G01N33/4925 ,  A61B5/0836

    摘要： A method of determining the mixed venous PCO.sub.2 (PvCO.sub.2) is disclosed, the method comprising the steps of:(a) measuring the PCO.sub.2 of the gases inhaled and exhaled by the patient under controlled conditions without rebreathing;(b) causing the patient to inhale a test gas containing at least a small concentration of CO.sub.2 and continuing to measure the PCO.sub.2 of the inspired and expired gases, the patient taking at least two breaths without rebreathing;(c) determining the PCO.sub.2 of the inspried gases (P.sub.I CO.sub.2) and the end tidal PCO.sub.2 of expired gases (PECO.sub.2);(d) determing the differences between the end tidal PCO.sub.2 and inspired PCO.sub.2 under control and test conditions, and relating it to the inspired PCO.sub.2 used in the determination of the difference. This relationship can be used to calculate the mixed venous PCO.sub.2.

    摘要翻译： 公开了一种测定混合静脉PCO2（PvCO2）的方法，所述方法包括以下步骤：（a）测量患者在受控条件下吸入和呼出的气体的PCO2，而无需再呼吸; （b）使患者吸入含有至少少量二氧化碳的测试气体，并继续测量启发和呼气的PCO2，患者至少进行两次呼吸，无需再呼吸; （c）确定呼出气体（PECO2）的气体（PICO2）和末端潮气PCO2的PCO2; （d）在控制和测试条件下确定末端潮汐PCO2和启发PCO2之间的差异，并将其与用于确定差异的灵敏PCO2相关。 这种关系可用于计算混合静脉PCO2。