公开(公告)号：US20150246146A1

公开(公告)日：2015-09-03

申请号：US14425114

申请日：2013-10-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brian Agnew ,  Robert Aggeler ,  Hee Chol Kang ,  Aimei Chen ,  Kyle Gee

IPC: A61K51/10 ,  C12P21/06 ,  C12P21/00 ,  G01N33/58 ,  C07K14/435 ,  C07K16/18

CPC classification number: A61K51/10 ,  C07K14/435 ,  C07K16/18 ,  C12P21/005 ,  C12P21/06 ,  G01N33/534 ,  G01N33/582 ,  G01N33/6854

Abstract: Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore.

Abstract translation: 本文提供了用于糖蛋白的位点特异性标记的方法，组合物和试剂盒，其包括酶介导的包含化学处理和环加成化学的修饰的糖的组合与包含反应性基团的标记分子，金属离子螯合剂， 和/或荧光团。

公开(公告)号：US20150079622A1

公开(公告)日：2015-03-19

申请号：US14384843

申请日：2013-02-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen Hu ,  Aimei Chen ,  Hee Chol Kang ,  Kyle Gee ,  Bhaskar Mandavilli

IPC: G01N33/52 ,  C07D417/06

CPC classification number: G01N33/84 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C09B23/06 ,  G01N33/52

Abstract: Disclosed herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced nucleic acid binding cyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.

Abstract translation: 本文公开了通过常规荧光显微镜，荧光光谱，流式细胞术和/或高含量成像检测活性氧（ROS）的化合物，组合物，方法和试剂盒。 本文公开的化合物是新的还原性核酸结合花青染料，其染料是用于检测ROS的探针，并测定体外和/或体内细胞中的氧化应激。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的新型还原染料即ROS探针的方法。

公开(公告)号：US10018617B2

公开(公告)日：2018-07-10

申请号：US15304800

申请日：2015-05-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Hee Chol Kang ,  Wenjun Zhou ,  Bradley Dubbels ,  Michael Olszowy ,  Michael O'Grady

IPC: G01N33/50 ,  C07D471/04 ,  C07C309/61 ,  C07K16/28 ,  C09B11/28

CPC classification number: G01N33/5005 ,  C07C309/61 ,  C07C2603/24 ,  C07D471/04 ,  C07K16/2812 ,  C09B11/28 ,  G01N33/5014 ,  G01N33/5044

Abstract: Described herein are fluorescent compounds and methods and comprising these compounds. The compounds disclosed herein are carbopyronine reagents that fluoresce in the red portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, and good retention within live cells as well as low toxicity toward cells.

公开(公告)号：US09828453B2

公开(公告)日：2017-11-28

申请号：US15212532

申请日：2016-07-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini Hofslokken Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven M. Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/10 ,  C08F230/08 ,  C07F7/18 ,  C08F222/38 ,  C08F220/58 ,  C08F236/20 ,  C08K3/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：US20170108518A1

公开(公告)日：2017-04-20

申请号：US15340134

申请日：2016-11-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen HU ,  Aimei Chen ,  Hee Chol Kang ,  Kyle Gee ,  Bhaskar Mandavilli

IPC: G01N33/84 ,  C09B23/06 ,  G01N33/52 ,  C09B23/04

CPC classification number: G01N33/84 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C09B23/06 ,  G01N33/52

Abstract: Disclosed herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced nucleic acid binding cyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.

公开(公告)号：US20160356782A1

公开(公告)日：2016-12-08

申请号：US15176872

申请日：2016-06-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Aimei CHEN ,  Kyle Gee ,  Hee Chol Kang

IPC: G01N33/64 ,  C09K11/06 ,  G01N21/64 ,  C09B11/24 ,  G01N33/50

CPC classification number: G01N33/64 ,  C07D311/82 ,  C07D471/06 ,  C07F5/022 ,  C07F9/5442 ,  C07F9/65522 ,  C09B11/24 ,  C09K11/06 ,  C09K2211/10 ,  C09K2211/1007 ,  C09K2211/1014 ,  C09K2211/1022 ,  C09K2211/1033 ,  C09K2211/1055 ,  C09K2211/1088 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/50 ,  G01N33/52 ,  G01N2021/6439 ,  Y10T436/143333

Abstract: The present disclosure is directed to fluorogenic schiff base-forming dyes capable of detecting analytes containing aldehyde and ketone groups. The dyes contain nucleophilic hydrazinyl appendages and are capable of binding and detecting analytes in situ.

公开(公告)号：US09440925B2

公开(公告)日：2016-09-13

申请号：US14810766

申请日：2015-07-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Aimei Chen ,  Hee Chol Kang

IPC: G01N33/532 ,  C07K1/13 ,  C07C309/42 ,  C07D225/08 ,  C07D225/02 ,  C07K16/18 ,  C07D223/08 ,  C07D223/26

CPC classification number: C07D225/08 ,  C07C309/42 ,  C07C2603/36 ,  C07D223/08 ,  C07D223/26 ,  C07D225/02 ,  C07K1/13 ,  C07K16/18 ,  G01N33/532

Abstract: The present disclosure is directed to a reactive ester agent for conjugating a click-reactive group to a carrier molecule or solid support. The reactive ester agent has the general formula IA, wherein the variables R1, R2, R3, Ra and L are described throughout the application.

公开(公告)号：US09400273B1

公开(公告)日：2016-07-26

申请号：US14801000

申请日：2015-07-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jixiang Liu ,  Hee Chol Kang ,  Kyle Gee ,  Christopher Langsdorf ,  Jolene Bradford ,  Gayle Buller

IPC: C12Q1/02 ,  G01N33/50 ,  C07D405/14 ,  C07D405/12

CPC classification number: G01N33/5008 ,  C07D405/12 ,  C07D405/14 ,  G01N33/533 ,  G01N2430/00

Abstract: Described herein are compounds, methods, and kits for long-term tracking of cell proliferation, differentiation, and/or function. The compounds of the present invention are novel cell-tracking reagents, efficiently excitable with a 405-nm violet laser, that provide bright fluorescence intensity, uniform cell staining, and good retention within cells as well as low toxicity toward cells.

Abstract translation: 本文描述了用于长期跟踪细胞增殖，分化和/或功能的化合物，方法和试剂盒。 本发明的化合物是能够以405nm紫激光激发的新型细胞追踪试剂，能够提供明亮的荧光强度，均匀的细胞染色和细胞内的良好保留以及对细胞的低毒性。