公开(公告)号：US20180230529A1

公开(公告)日：2018-08-16

申请号：US15891485

申请日：2018-02-08

Applicant: Life Technologies Corporation

Inventor： Jonathan SCHULTZ ,  Todd ROSWECH ,  Jon A. HOSHIZAKI ,  Albert L. CARRILLO ,  James A. BALL

IPC: C12Q1/6869 ,  B01F5/06 ,  G01N35/10 ,  B01F11/00 ,  B01L3/00 ,  B01F1/00 ,  G01N27/414 ,  C12Q1/6806

CPC classification number: C12Q1/6869 ,  B01F1/0027 ,  B01F5/0683 ,  B01F11/0048 ,  B01F11/0071 ,  B01L3/502 ,  B01L3/505 ,  B01L3/527 ,  B01L3/563 ,  B01L2200/04 ,  B01L2200/16 ,  B01L2300/022 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0683 ,  C12Q1/6806 ,  G01N27/4145 ,  G01N35/1002 ,  G01N35/1095 ,  Y10T436/11 ,  Y10T436/143333

Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.

公开(公告)号：US20160258011A1

公开(公告)日：2016-09-08

申请号：US15061273

申请日：2016-03-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Phil WAGGONER ,  James A. BALL ,  Wolfgang HINZ ,  Michael L. MINTO ,  Scott PARKER ,  David M. COX ,  Alexander MASTROIANNI ,  Jeremy GRAY ,  Marc GLAZER ,  Kimberly GORRELL

IPC: C12Q1/68 ,  G01N27/414

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

Abstract translation: 传感器装置包括基板，设置在基板上的半导体器件，具有表面电极结构的半导体器件和形成在表面电极结构上的糖类涂层。 在使用前可以除去糖衣。 该半导体器件还可以包括一个阱以及可选地布置在阱中的珠。

公开(公告)号：US20160003768A1

公开(公告)日：2016-01-07

申请号：US14789795

申请日：2015-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ronald L. CICERO ,  Marc GLAZER ,  Yufang WANG ,  Christina E. INMAN ,  Jeremy GRAY ,  Joseph KOSCINSKI ,  James A. BALL ,  Phil WAGGONER ,  Alexander MASTROIANNI

IPC: G01N27/414

Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.

Abstract translation: 传感器部件包括传感器，传感器包括与传感器协作的传感器表面和反应部位，并暴露传感器表面。 反应位点包括反应位点表面。 表面剂与反应位点表面或传感器表面结合。 表面活性剂包括在反应位点表面或传感器表面上与布朗斯台德碱或路易斯酸官能团反应的表面活性官能团，并且包括不具有供体电子对的远端官能团。

公开(公告)号：US20150252406A1

公开(公告)日：2015-09-10

申请号：US14721916

申请日：2015-05-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James A. BALL ,  David KUPEC ,  David MARRAN ,  Jon A. HOSHIZAKI

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6883 ,  G01N27/4141 ,  Y10T436/143333 ,  C12Q2527/119 ,  C12Q2565/607

Abstract: A method of sensing nucleotide reactions includes flowing at least one nucleotide solution from a container of at least two containers of a sensor system. The sensor system includes a sensor sensitive to a byproduct of nucleotide incorporation. Each container of the at least two containers includes a different nucleotide solution. The sensor system enters an idle mode after flowing. The method further includes cycling the at least two containers through at least two cycles. Each cycle includes depressurizing the at least two containers for a first period and pressurizing the at least two containers for a second period. The method also includes pressurizing the at least two containers when the sensor system enters an active mode.

Abstract translation: 感测核苷酸反应的方法包括从传感器系统的至少两个容器的容器流出至少一个核苷酸溶液。 传感器系统包括对核苷酸掺入的副产物敏感的传感器。 至少两个容器的每个容器都包含不同的核苷酸解决方案。 传感器系统在流动后进入空闲模式。 该方法还包括使至少两个容器循环至少两个循环。 每个循环包括使至少两个容器减压第一时间段，并且将至少两个容器加压第二周期。 该方法还包括当传感器系统进入活动模式时对至少两个容器进行加压。