公开(公告)号：US09156925B2

公开(公告)日：2015-10-13

申请号：US14082419

申请日：2013-11-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete I. Modahl ,  Nini H. Kjus ,  Astrid E. Molteberg ,  Diem Tran ,  Jo Aaserud ,  Talha M. Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F6/24 ,  C08F8/34 ,  C08F2/20 ,  C08F2/26 ,  C07F7/18 ,  C08F130/08 ,  C08F220/58 ,  C08F230/08 ,  C08F222/38 ,  C08F8/32 ,  C12Q1/68

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US10947333B2

公开(公告)日：2021-03-16

申请号：US16259963

申请日：2019-01-28

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete Irene Modahl ,  Nini Hofsloekken Kjus ,  Astrid Evenroed Molteberg ,  Diem Thuy Thi Tran ,  Jo Aaserud ,  Muhammed Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F230/08 ,  C08F220/58 ,  C08F8/34 ,  C08F8/32 ,  C08F8/12 ,  C08F2/20 ,  C08F130/08 ,  C08F6/24 ,  C07F7/18 ,  C12Q1/686 ,  C08F2/26 ,  C08F222/38 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/414 ,  C08J9/20

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US10941439B2

公开(公告)日：2021-03-09

申请号：US16176231

申请日：2018-10-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. Menchen ,  Guobing Xiang ,  Prasanna Thwar ,  Jeffrey Frediani ,  Allen Wong ,  Alfred Lui ,  Lily Lu

IPC: C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6874

Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

公开(公告)号：US10143990B2

公开(公告)日：2018-12-04

申请号：US15203582

申请日：2016-07-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander Mastroianni ,  Scott C. Benson ,  Steven M. Menchen ,  John Scott

IPC: B01J19/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6869

Abstract: A method of preparing a discrete polymer network array include mixing a plurality of nucleic acid polymer networks with a plurality of color-activated polymer networks to form a dispersion, applying the dispersion to an array of wells, the nucleic acid polymer networks selectively depositing into wells of the array of wells, and rinsing the array of wells to selectively remove the plurality of color-activated polymer networks.

公开(公告)号：US09938577B2

公开(公告)日：2018-04-10

申请号：US14855705

申请日：2015-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. Menchen ,  Alan Blanchard ,  Luisa Andruzzi ,  Shaheer H. Khan ,  Dmitriy Gremyachinskiy ,  Alfred Lui ,  Craig Stolarczyk ,  Tanya Sokolsky ,  Prasanna Krishnan Thwar

IPC: C12Q1/68 ,  C08F8/40

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

公开(公告)号：US09828453B2

公开(公告)日：2017-11-28

申请号：US15212532

申请日：2016-07-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini Hofslokken Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven M. Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/10 ,  C08F230/08 ,  C07F7/18 ,  C08F222/38 ,  C08F220/58 ,  C08F236/20 ,  C08K3/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：US09139666B2

公开(公告)日：2015-09-22

申请号：US14082368

申请日：2013-11-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete I. Modahl ,  Nini H. Kjus ,  Astrid E. Molteberg ,  Diem Tran ,  Jo Aaserud ,  Talha M. Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C12Q1/68 ,  C08F8/34 ,  C07F7/18 ,  C08F6/24 ,  C08F130/08 ,  C08F2/20 ,  C08F2/26 ,  C08F220/58 ,  C08F230/08 ,  C08F222/38 ,  C08F8/32

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

Abstract translation: 形成颗粒的方法包括在水性悬浮液中的分散相中聚合具有疏水保护基团的亲水性单体的多个聚合单元，从而形成包含多个疏水保护基团的聚合物颗粒。 该方法还包括将聚合物颗粒转化为亲水性颗粒。