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公开(公告)号：US06720191B1
公开(公告)日：2004-04-13
申请号：US09601559
申请日：2000-10-30
IPC分类号： G01N100
CPC分类号： G01N1/2813 , G01N35/00009 , G01N2001/282 , G01N2001/284 , Y10T436/11 , Y10T436/25
摘要： A method and apparatus of gathering by LCM identified cellular material from randorn locations on a tissue sample to designated locations on a transporting substrate enables convenient further processing. A transporting substrate has an identified mapped location for receiving identified cellular material. At least a segment of a selectively activatable coating is placed on the side of the transporting substrate in apposition to the tissue sample at the mapped location. The transporting substrate and sample are relatively moved to place the selectively activated coating at the mapped location in apposition to identified cellular material of the tissue sample which is to be extracted. Thereafter, the selectively activatable coating is activated and impressed or impressed and activated to form an adhesive region on the transporting substrate for adhering to the identified cellular material. Upon removal of the transporting substrate from the tissue sample, identified cellular material adheres to the transporting substrate at the mapped location.
摘要翻译： 通过LCM聚集的方法和装置将组织样本上的多个位置的细胞材料鉴定到运输基底上的指定位置，便于进一步处理。 传输基板具有用于接收所识别的细胞材料的识别的映射位置。 至少一段可选择性活化的涂层被放置在运送基片的侧面上，以与所述组织样本在所述位置相对应。 运输基质和样品被相对移动以将选择性活化的涂层置于映射位置处，以与待提取的组织样品的鉴定的细胞材料相关联。 此后，可选择性活化的涂层被活化并印刷或印刷并活化，以在传送基材上形成粘合区域，以粘附到所鉴定的多孔材料上。 当从组织样品中移走运送基质时，鉴定的细胞材料在映射位置附着于运输基质。
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公开(公告)号：US06420132B1
公开(公告)日：2002-07-16
申请号：US09495401
申请日：2000-01-31
IPC分类号： G01N130
CPC分类号： G01N1/2813 , G01N2001/2833 , G01N2001/284
摘要： Laser capture microdissection occurs where the transfer polymer film is placed on a substrate overlying visualized and selected cellular material from a sample for extraction. The transfer polymer film is focally activated (melted) with a pulse brief enough to allow the melted volume to be confined to that polymer directly irradiated. This invention uses brief pulses to reduce the thermal diffusion into surrounding non-irradiated polymer, preventing it from being heated hot enough to melt while providing sufficient heat by direct absorption in the small focal volume directly irradiated by the focused laser beam. This method can be used both in previously disclosed contact LCM, non contact LCM, using either condenser-side (or beam passes through polymer before tissue) or epi-irradiation (or laser passes through tissue before polymer). It can be used in configuration in which laser passes through tissue before polymer with and without an additional rigid substrate. In its preferred configuration it uses the inertial confinement of the surrounding unmelted thermoplastic polymer (and the overlying rigid substrate) to force expansion of the melted polymer into the underlying tissue target. Utilizing the short pulse protocol, the targeted and extracted material can have a diameter equal to or smaller than the exciting beam.
摘要翻译： 发生激光捕获显微切割，其中将转移聚合物膜放置在覆盖来自样品的可视化和选择的细胞材料的基底上用于提取。 转移聚合物膜以足够短的脉冲进行焦点活化（熔化），以使熔融体积被限制在直接照射的聚合物上。 本发明使用简短的脉冲来减少对周围未被照射的聚合物的热扩散，防止其被热加热至足以融化，同时通过直接吸收由聚焦激光束直接照射的小焦点体积提供足够的热量。 这种方法可以在以前公开的接触LCM，非接触式LCM中使用，使用冷凝器侧（或者束通过组织之前的聚合物）或外照射（或激光通过聚合物之前的组织）。 它可以用于在具有和不具有额外的刚性基底的聚合物之前激光穿过组织的配置。 在其优选的构造中，它使用周围未熔化的热塑性聚合物（和上覆的刚性基材）的惯性约束来强制将熔融的聚合物膨胀到下面的组织靶中。 利用短脉冲协议，目标和提取的材料的直径可以等于或小于激发光束。
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公开(公告)号：US06251467B1
公开(公告)日：2001-06-26
申请号：US09364617
申请日：1999-07-29
申请人： Lance A. Liotta , Michael Emmert-Buck , David B. Krizman , Rodrigo Chuaqui , W. Marston Linehan , Jeffry M. Trent , Robert F. Bonner , Seth R. Goldstein , Paul D. Smith , John I. Peterson
发明人： Lance A. Liotta , Michael Emmert-Buck , David B. Krizman , Rodrigo Chuaqui , W. Marston Linehan , Jeffry M. Trent , Robert F. Bonner , Seth R. Goldstein , Paul D. Smith , John I. Peterson
IPC分类号： B05D100
CPC分类号： G01N1/2813 , G01N1/04 , G01N1/08 , G01N1/2806 , G01N1/286 , G01N1/31 , G01N15/1468 , G01N33/50 , G01N2001/028 , G01N2001/2833 , G01N2001/284 , G01N2035/00237 , G02B21/32 , Y10T428/2813 , Y10T436/10
摘要： A method of microdissection which involves forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes a selectively activatable adhesive layer which provides, for example, chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated, the transfer surface and tissue sample are separated. During separation, the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample, the zone of cells of interest may then be molecularly analyzed.
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公开(公告)号：US5210161A
公开(公告)日：1993-05-11
申请号：US480460
申请日：1990-02-15
申请人： Paul D. Smith , Joel L. Martin
发明人： Paul D. Smith , Joel L. Martin
IPC分类号： C08F10/00
CPC分类号： C08F10/00
摘要： A vanadium dichloride-ether complex is carried on a porous support. This complex can be produced by either forming VCl.sub.2 and thereafter complexing the material with an ether or forming an ether complex with VCl.sub.4 or VCl.sub.3 and thereafter reducing the vanadium compound to VCl.sub.2. It is also possible to carry out the complexing reaction and the reduction essentially simultaneously. The resulting catalyst in combination with a cocatalyst is capable of giving high activity in olefin polymerization and demonstrates good sensitivity to molecular weight control agents, thus allowing the production of a broad spectrum of polymers so far as molecular weight is concerned.
摘要翻译： 二氯化二苯醚复合物载于多孔载体上。 该络合物可以通过形成VCl 2并随后用醚络合材料或与VCl 4或VCl 3形成醚络合物，然后将钒化合物还原成VCl 2来制备。 也可以基本上同时进行络合反应和还原。 所得催化剂与助催化剂组合能够在烯烃聚合中产生高活性，并且对分子量控制剂具有良好的敏感性，从而允许在分子量方面生产广谱聚合物。
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公开(公告)号：US5043514A
公开(公告)日：1991-08-27
申请号：US190741
申请日：1988-05-05
CPC分类号： B01J31/143 , B01J21/08 , B01J23/22 , B01J31/0212 , B01J31/26 , B01J37/0209 , C07C2/30 , B01J21/06 , B01J21/063 , B01J2231/12 , B01J2231/20 , C07C2521/04 , C07C2521/06 , C07C2521/08 , C07C2521/10 , C07C2521/12 , C07C2523/12 , C07C2527/167 , C07C2531/02 , C07C2531/14 , Y02P20/52
摘要： A process for dimerizing ethylene comprising contacting ethylene with an insoluble solid catalyst and a cocatalyst, said catalyst having at least on the surface thereof hydrocarbyl ortho ester groups bonded to vanadium or titanium.
摘要翻译： 一种使乙烯二聚的方法包括使乙烯与不溶性固体催化剂和助催化剂接触，所述催化剂至少在其表面具有与钒或钛键合的烃基原酸酯基团。
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公开(公告)号：US4681866A
公开(公告)日：1987-07-21
申请号：US887690
申请日：1986-07-21
申请人： Max P. McDaniel , Paul D. Smith
发明人： Max P. McDaniel , Paul D. Smith
CPC分类号： C08F10/00
摘要： Polymerization catalyst consisting essentially of treated silica support and zerovalent chromium compound is provided, as well as process for preparation and polymerization process with the invention catalyst. Silica treatment involves contacting a predominantly silica-containing support with at least one compound selected from the group consisting of Group IIA oxide or compounds convertible to the oxide form, a Group VA or VB oxide or compounds convertible to the oxide form or sulfuric acid, and calcining prior to contacting the treated support with a zerovalent chromium compound, thereby producing an active polymerization catalyst.
摘要翻译： 提供了基本上由处理的二氧化硅载体和零价铬化合物组成的聚合催化剂，以及本发明催化剂的制备和聚合方法。 二氧化硅处理包括使主要含二氧化硅的载体与至少一种选自IIA族氧化物或可转化为氧化物形式的化合物，VA族或VB族氧化物或可转化为氧化物形式或硫酸的化合物的化合物接触，以及 在将经处理的载体与零价铬化合物接触之前进行煅烧，从而制备活性聚合催化剂。
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公开(公告)号：US20130322490A1
公开(公告)日：2013-12-05
申请号：US13485700
申请日：2012-05-31
申请人： Kenneth Frazer Bell , Paul D. Smith
发明人： Kenneth Frazer Bell , Paul D. Smith
CPC分类号： G08B17/06 , G01D5/35316 , G01K11/3206
摘要： A method is presented for detecting an alarm condition with an optical fiber sensing system. An interrogator with a light source, a spectrometer, and a data processor is used to conduct a fast scan of a plurality of fiber optic sensing elements. First environmental parameter values are calculated for each fiber optic sensing element from spectrographic data collected by the interrogator during the first scan, and compared with a first threshold value. If the first environmental parameter value exceeds the first threshold value for any fiber optic sensing element, the fast scan is interrupted to perform a high resolution slow scan of that fiber optic sensing element. The optical fiber sensing system reports an alert if this high resolution slow scan indicates the alarm condition.
摘要翻译： 提出了一种利用光纤感测系统检测报警状态的方法。 使用具有光源，光谱仪和数据处理器的询问器来对多个光纤传感元件进行快速扫描。 从第一次扫描期间由询问器收集的光谱数据计算每个光纤感测元件的第一个环境参数值，并与第一个阈值进行比较。 如果第一环境参数值超过任何光纤感测元件的第一阈值，则快速扫描被中断以执行该光纤传感元件的高分辨率慢扫描。 如果这种高分辨率慢速扫描指示报警条件，则光纤传感系统报告警报。
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公开(公告)号：US20110085686A1
公开(公告)日：2011-04-14
申请号：US12900322
申请日：2010-10-07
申请人： Sanjay M. Bhandari , Paul D. Smith , Jiang (Jennifer) Yu , Sheng-Yu Peng , Priscilla E. Escobar-Bowser
发明人： Sanjay M. Bhandari , Paul D. Smith , Jiang (Jennifer) Yu , Sheng-Yu Peng , Priscilla E. Escobar-Bowser
CPC分类号： H04R29/006 , H04R3/005 , H04R25/407
摘要： A system includes a plurality of inputs each configured to receive a filtered version of a source signal. The system extracts the energy information from each input signal and compares the energy information of a plurality of input signals. Alternatively, the system extracts energy information from a signal that is the difference of two input signals. Based on the energy information, the system determines at least one parameter that may be changed in at least one circuit in a plurality of circuits to minimize the differences in energy of the input signals or to minimize the energy of the difference signal. Parameters may include for example amplification, delay, and corner frequency values.The set of circuits may include microphone interface circuits. Merely by way of example, a system with microphone interface circuits may be included in a hearing enhancement device or in a hands-free earpiece.
摘要翻译： 系统包括多个输入，每个输入被配置为接收源信号的滤波版本。 该系统从每个输入信号中提取能量信息，并比较多个输入信号的能量信息。 或者，系统从作为两个输入信号的差的信号提取能量信息。 基于能量信息，系统确定可以在多个电路中的至少一个电路中改变的至少一个参数，以最小化输入信号的能量差异或最小化差分信号的能量。 参数可以包括例如放大，延迟和转角频率值。 该组电路可以包括麦克风接口电路。 仅举例来说，具有麦克风接口电路的系统可以包括在听力增强装置中或免提耳机中。
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公开(公告)号：US07134976B1
公开(公告)日：2006-11-14
申请号：US10846750
申请日：2004-05-14
申请人： Paul D. Smith
发明人： Paul D. Smith
CPC分类号： A63B63/003 , A63B24/0021 , A63B63/00 , A63B63/004 , A63B2024/0037 , A63B2102/18 , A63B2102/24 , A63B2243/0025 , A63B2243/0066 , A63B2243/007
摘要： A training device comprising a target member, having a target face, a target arm and target support facilitates a training regiment for soccer, baseball, football and hockey players. The target support is coupled to a goal post or cross-bar of a goal. The target member is adjustably joined to the target support such that the target member and corresponding target face may be positioned in a plurality of locations without uncoupling the target support. The target face can be positioned in a plurality of positions throughout a vertical plane and may also be adjusted in a direction parallel to the target arm. The adjustability of the target face provides a versatile training device which can be arranged in numerous configurations to maintain fresh training regiments. In practice, multiple training devices maximize the effectiveness of the training. Other features include target face illumination means, counters and speakers for transmitting audio outputs.
摘要翻译： 包括具有目标面部，目标臂和目标支撑物的目标成员的训练装置有助于足球，棒球，足球和曲棍球运动员的训练团。 目标支持与目标职位或目标的交叉杆相结合。 目标构件可调节地连接到目标支撑件，使得目标构件和对应的目标面可以定位在多个位置，而不使目标支撑件解耦。 目标面可以定位在整个垂直平面中的多个位置，并且还可以在平行于目标臂的方向上进行调整。 目标面的可调性提供了一种通用的训练装置，可以配置多种构型，以维持新的训练团。 在实践中，多种训练装置最大限度地提高了训练的有效性。 其他功能包括目标面照明装置，用于传输音频输出的计数器和扬声器。
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公开(公告)号：US6137009A
公开(公告)日：2000-10-24
申请号：US416543
申请日：1999-10-12
IPC分类号： C07C209/08 , C07C211/29 , C07C209/00
CPC分类号： C07C209/08
摘要： The invention is a process to manufacture the intermediate secondary amine N-ethyl-2-chloro-6-fluoro-benzylamine, and then the process of reacting this intermediate to manufacture the herbicide flumetralin. Equimolar quantities of monoethylamine, sodium hydroxide, and 2-chloro-6-fluorobenzyl chloride are reacted at a temperature between about 70.degree. C. and about 100.degree. C. in a composition containing at least 2.5 times the required quantity of monoethylamine. The reagent monoethylamine functions as solvent and heat sink for the reaction, and also minimizes the formation of undesired byproducts. The excess monoethylamine is removed after formation of the intermediate. Then, equimolar quantities of sodium hydroxide in water and molten 4-chloro-3-5-dinitrobenzotrifluoride are added to the intermediate, and the temperature is controlled between about 90.degree. C. and about 115.degree. C. The product of this reaction is relatively pure, i.e., 98 percent by weight, molten flumetralin. It is advantageous to wash the product with boiling water to facilitate removal of salt and excess sodium hydroxide.
摘要翻译： 本发明是制备中间仲胺N-乙基-2-氯-6-氟 - 苄胺的方法，然后是使该中间体反应以制备除草剂氟马特林的方法。 将等摩尔量的单乙胺，氢氧化钠和2-氯-6-氟苄基氯在约70℃至约100℃的温度下在含有至少2.5倍所需量的单乙胺的组合物中反应。 试剂单乙胺用作反应的溶剂和散热器，并且还使不期望的副产物的形成最小化。 在形成中间体之后除去过量的单乙胺。 然后，将等摩尔量的氢氧化钠水溶液和熔融的4-氯-3,5-二硝基三氟甲苯加入到中间体中，温度控制在约90℃至约115℃之间。该反应的产物相对 纯的，即98重量％的熔融氟汞。 用沸水洗涤产品以便于除去盐和过量的氢氧化钠是有利的。
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