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13 条记录 (耗时 0.044 秒)

    Target activated microtransfer
    4.
    发明申请
    Target activated microtransfer 有权

    公开(公告)号：US20060134692A1

    公开(公告)日：2006-06-22

    申请号：US10543218

    申请日：2003-07-23

    申请人： Michael Emmert-Buck ,  Michael Tangrea ,  Robert Bonner ,  Rodrigo Chuaqui

    发明人： Michael Emmert-Buck ,  Michael Tangrea ,  Robert Bonner ,  Rodrigo Chuaqui

    IPC分类号： G01N33/53 ,  G01N33/537 ,  G01N33/543

    CPC分类号： G01N33/56966 ,  G01N33/543 ,  Y10T428/2813 ,  Y10T428/2839

    摘要： A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target). The immunoreagent can alter the transfer surface directly (for example with a heat generating enzyme carried by the immunoreagent), or indirectly (for example by changing a characteristic of the target). In some embodiments, the immunoreagent deposits a precipitate in the target that increases its light absorption relative to surrounding tissue, such that the biological specimen can be exposed to light to selectively heat the target. Alternatively, the immunoreagent is an immunofluorescent agent that carries a fluorophore that absorbs light and emits heat.

    Target activated microtransfer
    5.
    发明申请
    Target activated microtransfer 有权

    公开(公告)号：US20060172278A1

    公开(公告)日：2006-08-03

    申请号：US11202848

    申请日：2005-08-12

    申请人： Robert Bonner ,  Thomas Pohida ,  Michael Emmert-Buck ,  Michael Tangrea ,  Rodrigo Chuaqui

    发明人： Robert Bonner ,  Thomas Pohida ,  Michael Emmert-Buck ,  Michael Tangrea ,  Rodrigo Chuaqui

    IPC分类号： C12Q1/00 ,  G01N1/30 ,  C12M1/34

    CPC分类号： G01N33/543 ,  G01N33/56966 ,  G01N2001/284 ,  Y10T428/2813

    摘要： A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.

    Methods for the isolation and analysis of cellular protein content
    6.
    发明授权
    Methods for the isolation and analysis of cellular protein content 有权
    分离和分析细胞蛋白质含量的方法

    公开(公告)号：US06969614B1

    公开(公告)日：2005-11-29

    申请号：US09913667

    申请日：2000-02-16

    申请人： Lance A. Liotta ,  Nicole Simone ,  Michael Emmert-Buck ,  Emmanuel F. Petricoin III

    发明人： Lance A. Liotta ,  Nicole Simone ,  Michael Emmert-Buck ,  Emmanuel F. Petricoin III

    IPC分类号： G01N1/18 ,  G01N1/28 ,  G01N1/30 ,  G01N21/00 ,  G01N21/75 ,  G01N33/53 ,  G01N33/68

    CPC分类号： G01N33/6803 ,  G01N2001/284 ,  Y10T436/25375 ,  Y10T436/255

    摘要： The present invention describes devices and methods for performing protein analysis on laser capture microdissected cells, which permits proteomic analysis on cells of different populations. Particular disclosed examples are analysis of normal versus malignant cells, or a comparison of differential protein expression in cells that are progressing from normal to malignant. The protein content of the microdissected cells may be analyzed using techniques such as immunoassays, 1D and 2D gel electrophoresis characterization, Western blotting, liquid chromatography quadrapole ion trap electrospray (LCQ-MS), Matrix Assisted Laser Desorption Ionization/Time of Flight (MALDI/TOF), and Surface Enhanced Laser Desorption Ionization Spectroscopy (SELDI). In addition to permitting direct comparison of qualitative and quantitative protein content of tumor cells and normal cells from the same tissue sample, the methods also allow for investigation of protein characteristics of tumor cells, such as binding ability and amino acid sequence, and differential expression of proteins in particular cell populations in response to drug treatment. The present methods also provide, through the use of protein fingerprinting, a rapid and reliable way to identify the source tissue of a tumor metastasis.

    摘要翻译： 本发明描述了用于对激光捕获显微切割细胞进行蛋白质分析的装置和方法，其允许对不同群体的细胞进行蛋白质组学分析。 特别公开的实例是正常与恶性细胞的分析，或从正常到恶性进展的细胞中差异蛋白表达的比较。 可以使用免疫测定，1D和2D凝胶电泳表征，Western印迹，液相色谱四极离子阱电喷雾（LCQ-MS），基质辅助激光解吸电离/飞行时间（MALDI / TOF）和表面增强激光解吸电离光谱（SELDI）。 除了允许直接比较来自相同组织样品的肿瘤细胞和正常细胞的定性和定量蛋白质含量之外，该方法还允许研究肿瘤细胞的蛋白质特征，例如结合能力和氨基酸序列，以及差异表达 蛋白质在特定的细胞群体响应药物治疗。 本方法还通过使用蛋白质指纹图谱提供了一种快速可靠的方法来鉴定肿瘤转移的源组织。

    PB39, a gene dysregulated in prostate cancer, and uses thereof
    7.
    发明申请
    PB39, a gene dysregulated in prostate cancer, and uses thereof 审中-公开
    PB39，前列腺癌失调的基因及其用途

    公开(公告)号：US20060292627A1

    公开(公告)日：2006-12-28

    申请号：US11511259

    申请日：2006-08-29

    申请人： Rodrigo Chuaqui ,  Kristina Cole ,  Lance Liotta ,  Michael Emmert-Buck

    发明人： Rodrigo Chuaqui ,  Kristina Cole ,  Lance Liotta ,  Michael Emmert-Buck

    IPC分类号： C12Q1/68 ,  G01N33/574 ,  C07H21/04 ,  C12P21/06 ,  C07K16/30 ,  C07K14/82

    CPC分类号： C07K14/47 ,  C12Q1/6886 ,  C12Q2600/112

    摘要： A novel gene, PB39, that is up-regulated, or over-expressed, in prostate cancer has been identified. The gene has been identified by means of its cDNA obtained by reverse transcription of the corresponding mRNA. Microdissection of prostate glands that had been surgically removed from prostate cancer patients revealed a novel up-regulated transcript in an aggressive prostate carcinoma. Differential analysis for the presence of this gene was carried out from the same glands by comparing tanscription in microdissected normal prostatic epithelium versus that in microdissected invasive tumor. The transcript was over-expressed in 5 of 10 prostate carcinomas examined. A variant transcript was over-expressed in 4 of 4 prostate carcinomas, and was found in 1 of 4 normal samples. The invention provides a purified and isolated nucleic acid that includes the sequence of PB39 or its complement, the sequence of a variant of PB39 or its complement, and a primer or probe, that includes a sequence that is a fragment of these sequences. Additionally, the polypeptide encoded by these genes, an antibody to the polypeptide, and methods of detection of PB39 or its gene product are provided.

    摘要翻译： 已经鉴定出在前列腺癌中上调或过度表达的新基因PB39。 该基因已通过其相应mRNA的逆转录获得的cDNA鉴定。 从前列腺癌患者手术切除的前列腺的显微解剖显示在侵袭性前列腺癌中有一种新的上调的转录物。 通过比较显微解剖正常前列腺上皮与显微解剖浸润性肿瘤中的变体，从同一腺体进行该基因存在的差异分析。 在检查的10个前列腺癌中的5个中，转录物过表达。 在4个前列腺癌中的4个中，变体转录物过表达，并且在4个正常样品中的1个中发现。 本发明提供了纯化和分离的核酸，其包括PB39或其互补序列，PB39或其互补序列变体的序列，以及引物或探针，其包括作为这些序列的片段的序列。 另外，提供了由这些基因编码的多肽，多肽的抗体，以及检测PB39或其基因产物的方法。

    Direct cell target analysis
    9.
    发明申请
    Direct cell target analysis 审中-公开
    直接细胞靶分析

    公开(公告)号：US20050176068A1

    公开(公告)日：2005-08-11

    申请号：US10511511

    申请日：2003-04-25

    申请人： Michael Emmert-Buck ,  Mamoun Ahram

    发明人： Michael Emmert-Buck ,  Mamoun Ahram

    IPC分类号： C12Q1/68 ,  G01N33/50 ,  G01N33/53 ,  G01N33/567 ,  G01N33/68 ,  H04B10/08 ,  H04B10/17

    CPC分类号： H04B10/0731 ,  G01N33/5091 ,  G01N33/567 ,  G01N33/6857 ,  G01N2333/908 ,  H04B10/2916

    摘要： Disclosed herein are Direct Cell Target Analysis (“DCTA”) molecules and Direct Cell Target (“DCT”) methods for directly targeting and acting upon biomolecules. These methods and molecules can be used with specific cells within complex, heterogeneous tissue such that target biomolecules can be procured for subsequent analysis or directly analyzed without the need for physical separation of the biomolecules from other cells or cell components in the population. In general, the methods involve use of a fusion molecule having a first moiety to identify and localize target cells within a tissue sample, and a second moiety to generate detectable products within the target cells that may be detected and subsequently analyzed, and optionally isolated.

    摘要翻译： 本文公开了用于直接靶向和作用于生物分子的直接细胞靶分析（“DCTA”）分子和直接细胞靶（“DCT”）方法。 这些方法和分子可以与复杂的异质组织中的特定细胞一起使用，使得靶生物分子可以被采购用于随后的分析或直接分析，而不需要生物分子与群体中其他细胞或细胞组分的物理分离。 通常，所述方法包括使用具有第一部分的融合分子来鉴定和定位组织样品中的靶细胞，以及第二部分以在靶细胞内产生可被检测和随后分析并任选分离的可检测产物。