公开(公告)号：US06867038B2

公开(公告)日：2005-03-15

申请号：US09780234

申请日：2001-02-09

申请人： Lance A. Liotta ,  Michael Emmert-Buck ,  David B. Krizman ,  Rodrigo Chuaqui ,  W. Marston Linehan ,  Jeffry M. Trent ,  Robert F. Bonner ,  Seth R. Goldstein ,  Paul D. Smith ,  John I. Peterson

发明人： Lance A. Liotta ,  Michael Emmert-Buck ,  David B. Krizman ,  Rodrigo Chuaqui ,  W. Marston Linehan ,  Jeffry M. Trent ,  Robert F. Bonner ,  Seth R. Goldstein ,  Paul D. Smith ,  John I. Peterson

IPC分类号： C12M1/26 ,  C12M1/34 ,  G01N1/02 ,  G01N1/04 ,  G01N1/08 ,  G01N1/28 ,  G01N1/31 ,  G01N15/14 ,  G01N33/50 ,  G01N35/00 ,  G02B21/32 ,  C12N5/00

CPC分类号： G01N1/2813 ,  G01N1/04 ,  G01N1/08 ,  G01N1/2806 ,  G01N1/286 ,  G01N1/31 ,  G01N15/1468 ,  G01N33/50 ,  G01N2001/028 ,  G01N2001/2833 ,  G01N2001/284 ,  G01N2035/00237 ,  G02B21/32 ,  Y10T428/2813 ,  Y10T436/10

摘要： A method of microdissection which involves forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes a selectively activatable adhesive layer which provides, for example, chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated, the transfer surface and tissue sample are separated. During separation, the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample, the zone of cells of interest may then be molecularly analyzed.

摘要翻译： 一种显微解剖的方法，其包括使用显微镜形成组织样本的细胞的图像场，从细胞的图像区鉴定至少一个感兴趣区域的至少一个感兴趣区域，所述细胞的至少一个区域包括不同类型的细胞， 并且从所述组织样品中提取所述感兴趣的细胞的至少一个区域。 通过使组织样品与可以选择性活化的转移表面接触使得其区域粘附到待提取的感兴趣的细胞区域来实现提取。 转移表面包括可选择性活化的粘合剂层，其提供例如化学或静电附着到组织样品的选定区域。 在转印表面被激活之后，转印表面和组织样品被分离。 在分离期间，感兴趣的细胞区保持粘附到转移表面，并因此与组织样品分离，然后可以分析分析感兴趣的细胞区域。

公开(公告)号：US06251516B1

公开(公告)日：2001-06-26

申请号：US09018596

申请日：1998-02-04

申请人： Robert F. Bonner ,  Lance A. Liotta ,  Michael Emmert-Buck ,  David B. Krizman ,  Rodrigo Chuaqui ,  W. Marston Linehan ,  Jeffry M. Trent ,  Seth R. Goldstein ,  Paul D. Smith ,  John I. Peterson

发明人： Robert F. Bonner ,  Lance A. Liotta ,  Michael Emmert-Buck ,  David B. Krizman ,  Rodrigo Chuaqui ,  W. Marston Linehan ,  Jeffry M. Trent ,  Seth R. Goldstein ,  Paul D. Smith ,  John I. Peterson

IPC分类号： B32B712

CPC分类号： G01N1/2813 ,  G01N1/04 ,  G01N1/08 ,  G01N1/2806 ,  G01N1/286 ,  G01N1/31 ,  G01N15/1468 ,  G01N33/50 ,  G01N2001/028 ,  G01N2001/2833 ,  G01N2001/284 ,  G01N2035/00237 ,  G02B21/32 ,  Y10T428/2813 ,  Y10T428/2839

摘要： A method of microdissection which involves forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes a selectively activatable adhesive layer which provides, for example, chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated, the transfer surface and tissue sample are separated. During separation, the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample, the zone of cells of interest may then be molecularly analyzed.

摘要翻译： 一种显微解剖的方法，其包括使用显微镜形成组织样本的细胞的图像场，从细胞的图像区鉴定至少一个感兴趣区域的至少一个感兴趣区域，所述细胞的至少一个区域包括不同类型的细胞， 并且从所述组织样品中提取所述感兴趣的细胞的至少一个区域。 通过使组织样品与可以选择性活化的转移表面接触使得其区域粘附到待提取的感兴趣的细胞区域来实现提取。 转移表面包括可选择性活化的粘合剂层，其提供例如化学或静电附着到组织样品的选定区域。 在转印表面被激活之后，转印表面和组织样品被分离。 在分离期间，感兴趣的细胞区保持粘附到转移表面，并因此与组织样品分离，然后可以分析分析感兴趣的细胞区域。

公开(公告)号：US06251467B1

公开(公告)日：2001-06-26

申请号：US09364617

申请日：1999-07-29

申请人： Lance A. Liotta ,  Michael Emmert-Buck ,  David B. Krizman ,  Rodrigo Chuaqui ,  W. Marston Linehan ,  Jeffry M. Trent ,  Robert F. Bonner ,  Seth R. Goldstein ,  Paul D. Smith ,  John I. Peterson

发明人： Lance A. Liotta ,  Michael Emmert-Buck ,  David B. Krizman ,  Rodrigo Chuaqui ,  W. Marston Linehan ,  Jeffry M. Trent ,  Robert F. Bonner ,  Seth R. Goldstein ,  Paul D. Smith ,  John I. Peterson

IPC分类号： B05D100

CPC分类号： G01N1/2813 ,  G01N1/04 ,  G01N1/08 ,  G01N1/2806 ,  G01N1/286 ,  G01N1/31 ,  G01N15/1468 ,  G01N33/50 ,  G01N2001/028 ,  G01N2001/2833 ,  G01N2001/284 ,  G01N2035/00237 ,  G02B21/32 ,  Y10T428/2813 ,  Y10T436/10

摘要： A method of microdissection which involves forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes a selectively activatable adhesive layer which provides, for example, chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated, the transfer surface and tissue sample are separated. During separation, the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample, the zone of cells of interest may then be molecularly analyzed.