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公开(公告)号：US11118215B2
公开(公告)日：2021-09-14
申请号：US10572644
申请日：2004-09-17
IPC分类号： C40B20/04 , C12Q1/6837 , C12N15/10 , C12Q1/6811
摘要： In one aspect, the present invention relates to a method for obtaining structural information about an encoded molecule. The encoded molecule may be produced by a reaction of a plurality of chemical entities and may be capable of being connected to an identifier oligonucleotide containing codons informative of the identity of the chemical entities which have participated in the formation of the encoded molecule. In a certain embodiment, primers are designed complementary to the codons appearing on the identifier oligonucleotide, and the presence, absence or relative abundance of a codon is evaluated by mixing a primer with the identifier oligonucleotide in the presence of a polymerase and substrate (deoxy)ribonucleotide triphosphates and measuring the extension reaction. In another aspect, the invention provides a method for selecting compounds which binds to a target. More specifically, the invention relates to a method in which a target associated with an oligonucleotide initially is mixed with a library of complexes, each complex comprising a display molecule and an oligonucleotide identifying said display molecule. Next, due an increased proximity, the target oligonucleotide is coupled to the identifier oligonucleotide of complexes having a display mole-cute with affinity towards the target. In a final stage the coupled nucleotides are analysed to deduce at least the identity of the display molecule.
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公开(公告)号：US09096951B2
公开(公告)日：2015-08-04
申请号：US13179283
申请日：2011-07-08
CPC分类号： C40B40/08 , B01J19/0046 , B01J2219/00592 , C12N15/1068 , C12Q1/6811 , C40B50/04 , C40B50/06 , C40B50/10 , C12Q2565/501 , C12Q2531/113
摘要： The present invention relates to a method for generating a second-generation library. In a first step, a library of encoded molecules associated with an identifier nucleic acid comprising codons identifying chemical entities that have participated in the formation of the encoded molecule is provided. In a second step, the library is partitioned and encoded molecules having a certain property are selected. Codons of identifiers of selected encoded molecules are subsequently identified, and a second-generation library is prepared using at least some of the chemical entities coded for by the identified codons. The new focussed library may be used for another partition step to select encoded molecules with a certain property.
摘要翻译： 本发明涉及生成第二代文库的方法。 在第一步中，提供了与标识符核酸相关联的编码分子的文库，其包含鉴定参与编码分子形成的化学实体的密码子。 在第二步中，分离文库，并选择具有一定性质的编码分子。 随后鉴定所选编码分子的标识符的密码子，并且使用由鉴定的密码子编码的至少一些化学实体来制备第二代文库。 新的聚焦库可以用于另一个分割步骤以选择具有一定特性的编码分子。
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公开(公告)号：US20120208251A1
公开(公告)日：2012-08-16
申请号：US13455905
申请日：2012-04-25
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C12P7/06 , C12P7/14 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
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公开(公告)号：US07713723B1
公开(公告)日：2010-05-11
申请号：US12645116
申请日：2009-12-22
IPC分类号： C12N9/28
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C11D3/38681 , C12P7/14 , C12P19/02 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.
摘要翻译： 本发明涉及母体类戊酰样α-淀粉酶的变体（突变体），该变体具有α-淀粉酶活性并且表现出改变的稳定性，特别是在高温和/或低pH相对和/或低Ca 2+下 亲本α-淀粉酶。
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公开(公告)号：US07601529B2
公开(公告)日：2009-10-13
申请号：US10513951
申请日：2003-05-14
申请人： Sanne Schroder Glad , Carsten Andersen , Torben Vedel Borchert , Katja Salomon Johansen , Henrik Frisner , Thomas Thisted , Mads Eskelund Bjornvad
发明人： Sanne Schroder Glad , Carsten Andersen , Torben Vedel Borchert , Katja Salomon Johansen , Henrik Frisner , Thomas Thisted , Mads Eskelund Bjornvad
CPC分类号： C12N9/88 , C11D3/38636
摘要： The present invention relates to pectate lyase variants exhibiting alterations relative to a parent enzyme exhibiting pectate lyase activity as its major enzymatic activity; to a method of producing such enzymes; and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries. Compared to the parent enzyme, the pectate lyase variants of the present invention exhibit improved stability in detergents.
摘要翻译： 本发明涉及相对于显示出果胶裂解酶活性的亲本酶作为其主要酶活性的改变的果胶酸裂解酶变体; 涉及一种生产这种酶的方法; 以及在纺织，洗涤剂和纤维素纤维加工业中使用这些酶的方法。 与亲本酶相比，本发明的果胶酸裂解酶变体表现出改进的洗涤剂稳定性。
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公开(公告)号：US20060166197A1
公开(公告)日：2006-07-27
申请号：US10507842
申请日：2003-03-14
申请人： Alex Gouliaev , Justin Ho , Jakob Felding , Christian Sams , Henrik Pedersen , Kim Jensen , Anders Hansen , Mikkel Lundorf , Gitte Husemoen , Thomas Franch , Thomas Thisted
发明人： Alex Gouliaev , Justin Ho , Jakob Felding , Christian Sams , Henrik Pedersen , Kim Jensen , Anders Hansen , Mikkel Lundorf , Gitte Husemoen , Thomas Franch , Thomas Thisted
IPC分类号： C40B40/08 , C07H21/02 , C07F9/6512
CPC分类号： C07D405/04 , C07B2200/11 , C07F9/65586 , C07F9/65616 , C07H21/00 , C07H21/02 , C07H23/00 , C12N15/1068 , C12P19/34 , C40B40/00
摘要： A building block having the dual capabilities of transferring the genetic information e.g. by recognising an encoding element and transferring a functional entity to a recipient reactive group is disclosed. The building block can be designed with an adjustable transferability taking into account the components of the building block. The building block may be used in the generation of a single complex or libraries of different complexes, wherein the complex comprises an encoded molecule linked to an encoding element. Libraries of complexes are useful in the quest for pharmaceutically active compounds.
摘要翻译： 具有转移遗传信息的双重能力的构建块，例如， 通过识别编码元件并将功能实体传送到接收者反应组。 构建块可以设计成具有可调节的可转移性，同时考虑到构件的部件。 构建单元可以用于生成单个复合体或不同复合物的文库，其中复合物包含与编码元件连接的编码分子。 复合物库可用于寻求药物活性化合物。
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公开(公告)号：US20050250663A1
公开(公告)日：2005-11-10
申请号：US10630203
申请日：2003-07-29
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C11D3/38681 , C12P7/14 , C12P19/02 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.
摘要翻译： 本发明涉及母体类戊酰样α-淀粉酶的变体（突变体），该变体具有α-淀粉酶活性并且表现出改变的稳定性，特别是在高温和/或低pH相对和/或低Ca 2+下 亲本α-淀粉酶。
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公开(公告)号：US09574189B2
公开(公告)日：2017-02-21
申请号：US12095778
申请日：2006-12-01
申请人： Thomas Franch , Mikkel Dybro Lundorf , Søren Nyboe Jacobsen , Eva Kampmann Olsen , Anne Lee Andersen , Anette Holtmann , Anders Holm Hansen , Anders Malling Sørensen , Anne Goldbech , Daen de Leon , Ditte Kievsmose Kaldor , Frank Abildgaard Sløk , Gitte Nystrup Husemoen , Johannes Dolberg , Kim Birkebæ Jensen , Lene Pedersen , Mads Nørregaard-Madsen , Michael Anders Godskesen , Sanne Schrøder Glad , Søren Neve , Thomas Thisted , Tine Titilola Akinleminu Kronborg , Christian Klarner Sams , Jakob Felding , Per-Ola Freskgård , Alex Haahr Gouliaev , Henrik Pedersen
发明人： Thomas Franch , Mikkel Dybro Lundorf , Søren Nyboe Jacobsen , Eva Kampmann Olsen , Anne Lee Andersen , Anette Holtmann , Anders Holm Hansen , Anders Malling Sørensen , Anne Goldbech , Daen de Leon , Ditte Kievsmose Kaldor , Frank Abildgaard Sløk , Gitte Nystrup Husemoen , Johannes Dolberg , Kim Birkebæ Jensen , Lene Pedersen , Mads Nørregaard-Madsen , Michael Anders Godskesen , Sanne Schrøder Glad , Søren Neve , Thomas Thisted , Tine Titilola Akinleminu Kronborg , Christian Klarner Sams , Jakob Felding , Per-Ola Freskgård , Alex Haahr Gouliaev , Henrik Pedersen
CPC分类号： C12N15/1068 , C12N15/1089 , C40B40/08 , C40B50/04 , C40B50/06
摘要： Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reaction site with one or more reactants, and b) reacted enzymatically at the priming site with one or more tag(s) identifying the reactant(s).
摘要翻译： 公开了一种获得包含与单链标识符寡核苷酸连接的分子的双功能复合物的方法，其中包含化学反应位点的新生双功能复合物和用于酶标记添加的引发位点是a）在化学反应位点与 一种或多种反应物，和b）在起始位点用一种或多种标记反应物的标签进行酶促反应。
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公开(公告)号：US08563290B2
公开(公告)日：2013-10-22
申请号：US13605455
申请日：2012-09-06
申请人： Sanne Schroder Glad , Carsten Andersen , Torben Vedel Borchert , Katja Salomon Johansen , Henrik Frisner , Mads Eskelund Bjornvad , Thomas Thisted
发明人： Sanne Schroder Glad , Carsten Andersen , Torben Vedel Borchert , Katja Salomon Johansen , Henrik Frisner , Mads Eskelund Bjornvad , Thomas Thisted
CPC分类号： C12N9/88 , C11D3/38636
摘要： The present invention relates to pectate lyase variants exhibiting alterations relative to a parent enzyme exhibiting pectate lyase activity as its major enzymatic activity; to a method of producing such enzymes; and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries. Compared to the parent enzyme, the pectate lyase variants of the present invention exhibit improved stability in detergents.
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公开(公告)号：US08252573B2
公开(公告)日：2012-08-28
申请号：US13020545
申请日：2011-02-03
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618 , C12P7/06 , C12P7/14 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
摘要翻译： 本发明涉及亲本Termamyl样α-淀粉酶的变体（突变体），该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质。
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