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    Methods of using an array of pooled probes in genetic analysis
    22.
    发明授权
    Methods of using an array of pooled probes in genetic analysis 有权
    在遗传分析中使用汇集探针阵列的方法

    公开(公告)号：US06306643B1

    公开(公告)日：2001-10-23

    申请号：US09138958

    申请日：1998-08-24

    Applicant: Erik Gentalen ,  Mark Chee

    Inventor： Erik Gentalen ,  Mark Chee

    IPC: C12M134

    CPC classification number: C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Q2565/543 ,  C12Q2565/513

    Abstract: The invention provides arrays of polynucleotide probes having at least one pooled position. A typical array comprises a support having at least three discrete regions. A first region bears a pool of polynucleotide probes comprising first and second probes. A second region bears the first probe without the second probe and a third region bears the second probe without the first probe. A target nucleic acid having segments complementary to both the first and second probes shows stronger normalized binding to the first region than to the aggregate of binding to the second and third regions due to cooperative binding of pooled probes in the first region. The invention provide methods of using such arrays for e.g., linkage analysis, sequence analysis, and expression monitoring.

    Abstract translation: 本发明提供具有至少一个合并位置的多核苷酸探针阵列。 典型的阵列包括具有至少三个离散区域的支撑件。 第一区域具有包含第一和第二探针的多核苷酸探针池。 第二区域承载第一探针而没有第二探针，第三区域承载第二探针而没有第一探针。 具有与第一和第二探针两者互补的区段的靶核酸由于在第一区域中的合并探针的协同结合而显示比第一区域更强的标准化结合，而不是与第二和第三区域结合的聚集体。 本发明提供了使用这种阵列进行例如连锁分析，序列分析和表达监测的方法。

    Polymorphisms in the glucose-6 phosphate dehydrogenase locus
    25.
    发明授权
    Polymorphisms in the glucose-6 phosphate dehydrogenase locus 失效
    葡萄糖-6-磷酸脱氢酶基因座的多态性

    公开(公告)号：US5856104A

    公开(公告)日：1999-01-05

    申请号：US813508

    申请日：1997-03-07

    Applicant: Mark Chee ,  Jian-Bing Fan

    Inventor： Mark Chee ,  Jian-Bing Fan

    IPC: C12Q1/68 ,  C07H21/04

    CPC classification number: C12Q1/6827 ,  C12Q1/6876 ,  C12Q2600/156

    Abstract: The invention provides nucleic acid segments of the glucose-6 phosphate dehydrogenase locus of the human genome including polymorphic sites. Allele-specific primers and probes hybridizing to regions flanking these sites are also provided. The nucleic acids, primers and probes are used in applications such as forensics, paternity testing, medicine and genetic analysis.

    Abstract translation: 本发明提供人类基因组的葡萄糖-6-磷酸脱氢酶基因座的核酸片段，包括多态位点。 还提供了与这些位点侧翼区域杂交的等位基因特异性引物和探针。 核酸，引物和探针用于取证，亲子鉴定，药物和遗传分析等应用。

    Methods of using an array of pooled probes in genetic analysis
    27.
    发明授权
    Methods of using an array of pooled probes in genetic analysis 有权
    在遗传分析中使用汇集探针阵列的方法

    公开(公告)号：US08586312B2

    公开(公告)日：2013-11-19

    申请号：US12858237

    申请日：2010-08-17

    Applicant: Erik Gentalen ,  Mark Chee

    Inventor： Erik Gentalen ,  Mark Chee

    IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC classification number: C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Q2565/543 ,  C12Q2565/513

    Abstract: The invention provides arrays of polynucleotide probes having at least one pooled position. A typical array comprises a support having at least three discrete regions. A first region bears a pool of polynucleotide probes comprising first and second probes. A second region bears the first probe without the second probe and a third region bears the second probe without the first probe. A target nucleic acid having segments complementary to both the first and second probes shows stronger normalized binding to the first region than to the aggregate of binding to the second and third regions due to cooperative binding of pooled probes in the first region. The invention provides methods of using such arrays for e.g., linkage analysis, sequence analysis, and expression monitoring.

    Abstract translation: 本发明提供具有至少一个合并位置的多核苷酸探针阵列。 典型的阵列包括具有至少三个离散区域的支撑件。 第一区域具有包含第一和第二探针的多核苷酸探针池。 第二区域承载第一探针而没有第二探针，第三区域承载第二探针而没有第一探针。 具有与第一和第二探针两者互补的区段的靶核酸由于在第一区域中的合并探针的协同结合而显示比第一区域更强的标准化结合，而不是与第二和第三区域结合的聚集体。 本发明提供了使用这种阵列进行例如连锁分析，序列分析和表达监测的方法。

    Methods of enzymatic discrimination enhancement and surface bound double-stranded DNA
    30.
    发明申请
    Methods of enzymatic discrimination enhancement and surface bound double-stranded DNA 审中-公开
    酶鉴别增强方法和表面结合双链DNA

    公开(公告)号：US20060292579A1

    公开(公告)日：2006-12-28

    申请号：US11176012

    申请日：2005-07-05

    Applicant: David Lockhart ,  Mark Chee ,  Dirk Vetter ,  Martin Digglemann

    Inventor： David Lockhart ,  Mark Chee ,  Dirk Vetter ,  Martin Digglemann

    IPC: C12Q1/68

    CPC classification number: C12N15/1093 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00659 ,  B01J2219/00722 ,  C07B2200/11 ,  C07H21/00 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6837 ,  C12Q1/6874 ,  C40B40/06 ,  C40B80/00 ,  G01N33/5308 ,  G01N33/551

    Abstract: Methods for discriminating between fully complementary hybrids and those that differ by one or more base pairs and libraries of unimolecular, double-stranded oligonucleotides on a solid support. In one embodiment, the present invention provides methods of using nuclease treatment to improve the quality of hybridization signals on high density oligonucleotide arrays. In another embodiment, the present invention provides methods of using ligation reactions to improve the quality of hybridization signals on high density oligonucleotide arrays. In yet another embodiment, the present invention provides libraries of unimolecular or intermolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a related aspect, the present invention provides libraries of conformationally restricted probes on a solid support. The probes are restricted in their movement and flexibility using double-stranded oligonucleotides as scaffolding. The probes are also useful in various screening procedures associated with drug discovery and diagnosis. The present invention further provides methods for the preparation and screening of the above libraries.

    Abstract translation: 用于区分完全互补的杂交体与通过一个或多个碱基对不同的那些的方法和在固体支持物上的单分子双链寡核苷酸的文库。 在一个实施方案中，本发明提供了使用核酸酶处理来提高高密度寡核苷酸阵列上杂交信号质量的方法。 在另一个实施方案中，本发明提供了使用连接反应来提高高密度寡核苷酸阵列上杂交信号质量的方法。 在另一个实施方案中，本发明提供了在固体支持物上的单分子或分子间双链寡核苷酸的文库。 这些文库在药物发现中可用于筛选许多生物样品，用于双链寡核苷酸与肽，蛋白质，药物和RNA之间的特异性相互作用。 在相关方面，本发明提供了在固体支持物上的构象限制探针的文库。 使用双链寡核苷酸作为脚手架，探针的运动和灵活性受到限制。 探针也可用于与药物发现和诊断相关的各种筛选程序。 本发明还提供了制备和筛选上述文库的方法。

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