-
公开(公告)号：US20060229824A1
公开(公告)日：2006-10-12
申请号：US11367800
申请日：2006-03-03
申请人： Maureen Cronin , Charles Miyada , Earl Hubbell , Mark Chee , Stephen Fodor , Xiaohua Huang , Robert Lipshutz , Peter Lobban , MacDonald Morris , Edward Sheldon
发明人： Maureen Cronin , Charles Miyada , Earl Hubbell , Mark Chee , Stephen Fodor , Xiaohua Huang , Robert Lipshutz , Peter Lobban , MacDonald Morris , Edward Sheldon
IPC分类号： G06F19/00
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/00432 , B01J2219/00529 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00644 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6874 , C12Q1/6876 , C12Q2600/106 , C12Q2600/156 , C12Q2600/172 , C40B40/06 , C40B60/14
摘要： The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the biotransformation genes, such as cytochromes P450. For example, one such array comprises four probe sets. A first probe set comprises a plurality of probes, each probe comprising a segment of at least three nucleotides exactly complementary to a subsequence of a reference sequence from a biotransformation gene, the segment including at least one interrogation position complementary to a corresponding nucleotide in the reference sequence. Second, third and fourth probe sets each comprise a corresponding probe for each probe in the first probe set. The probes in the second, third and fourth probe sets are identical to a sequence comprising the corresponding probe from the first probe set or a subsequence of at least three nucleotides thereof that includes the at least one interrogation position, except that the at least one interrogation position is occupied by a different nucleotide in each of the four corresponding probes from the four probe sets.
摘要翻译： 本发明提供了固定化探针的阵列，以及采用该阵列的方法，用于检测生物转化基因如细胞色素P450中的突变。 例如，一个这样的阵列包括四个探针组。 第一探针组包含多个探针，每个探针包含与来自生物转化基因的参考序列的亚序列完全互补的至少三个核苷酸的片段，该片段包含与参考文献中的相应核苷酸互补的至少一个询问位置 序列。 第二，第三和第四探针组各自包括在第一探针组中的每个探针的相应探针。 第二，第三和第四探针组中的探针与包含来自第一探针组的相应探针或包含至少一个询问位置的至少三个核苷酸的亚序列的序列相同，除了至少一个询问 位置由来自四个探针组的四个相应探针中的每一个中的不同核苷酸占据。
-
公开(公告)号：US20070054286A1
公开(公告)日：2007-03-08
申请号：US11401482
申请日：2006-04-11
申请人： Maureen Cronin , Charles Miyada , Earl Hubbell , Mark Chee , Stephen Fodor , Xiaohua Huang , Robert Lipshutz , Peter Lobban , MacDonald Morris , Edward Sheldon
发明人： Maureen Cronin , Charles Miyada , Earl Hubbell , Mark Chee , Stephen Fodor , Xiaohua Huang , Robert Lipshutz , Peter Lobban , MacDonald Morris , Edward Sheldon
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/00432 , B01J2219/00529 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00644 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6874 , C12Q1/6876 , C12Q2600/106 , C12Q2600/156 , C12Q2600/172 , C40B40/06 , C40B60/14
摘要： The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the biotransformation genes, such as cytochromes P450. For example, one such array comprises four probe sets. A first probe set comprises a plurality of probes, each probe comprising a segment of at least three nucleotides exactly complementary to a subsequence of a reference sequence from a biotransformation gene, the segment including at least one interrogation position complementary to a corresponding nucleotide in the reference sequence. Second, third and fourth probe sets each comprise a corresponding probe for each probe in the first probe set. The probes in the second, third and fourth probe sets are identical to a sequence comprising the corresponding probe from the first probe set or a subsequence of at least three nucleotides thereof that includes the at least one interrogation position, except that the at least one interrogation position is occupied by a different nucleotide in each of the four corresponding probes from the four probe sets.
摘要翻译： 本发明提供了固定化探针的阵列，以及采用该阵列的方法，用于检测生物转化基因如细胞色素P450中的突变。 例如，一个这样的阵列包括四个探针组。 第一探针组包含多个探针，每个探针包含与来自生物转化基因的参考序列的亚序列完全互补的至少三个核苷酸的片段，该片段包含与参考文献中的相应核苷酸互补的至少一个询问位置 序列。 第二，第三和第四探针组各自包括在第一探针组中的每个探针的相应探针。 第二，第三和第四探针组中的探针与包含来自第一探针组的相应探针或包含至少一个询问位置的至少三个核苷酸的亚序列的序列相同，除了至少一个询问 位置由来自四个探针组的四个相应探针中的每一个中的不同核苷酸占据。
-
公开(公告)号：US20060110727A9
公开(公告)日：2006-05-25
申请号：US10418414
申请日：2003-04-18
申请人： Glenn McGall , Charles Miyada , Maureen Cronin , Jennifer Tan , Mark Chee
发明人： Glenn McGall , Charles Miyada , Maureen Cronin , Jennifer Tan , Mark Chee
IPC分类号： C12Q1/68 , C07H21/04 , C07D473/02 , C07D45/02
CPC分类号： C40B80/00 , B01J19/0046 , B01J2219/00432 , B01J2219/00529 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6832 , C40B40/06 , C40B60/14
摘要： Oligonucleotide analogue arrays attached to solid substrates and methods related to the use thereof are provided. The oligonucleotide analogues hybridize to nucleic acids with either higher or lower specificity than corresponding unmodified oligonucleotides. Target nucleic acids which comprise nucleotide analogues are bound to oligonucleotide and oligonucleotide analogue arrays.
摘要翻译： 提供了连接到固体底物的寡核苷酸类似物阵列和与使用相关的方法。 寡核苷酸类似物与具有比相应的未修饰寡核苷酸更高或更低特异性的核酸杂交。 包含核苷酸类似物的靶核酸与寡核苷酸和寡核苷酸类似物阵列结合。
-
公开(公告)号：US20050191646A1
公开(公告)日：2005-09-01
申请号：US10961341
申请日：2004-10-07
申请人： David Lockhart , Mark Chee , Kevin Gunderson , Lai Chaoqiang , Lisa Wodicka , Maureen Cronin , Danny Lee , Huu Tran , Hajime Matsuzaki , Glenn McGall , Anthony Barone
发明人： David Lockhart , Mark Chee , Kevin Gunderson , Lai Chaoqiang , Lisa Wodicka , Maureen Cronin , Danny Lee , Huu Tran , Hajime Matsuzaki , Glenn McGall , Anthony Barone
CPC分类号： C07H19/052 , C07H19/12 , C07H21/00 , C12Q1/6809 , C12Q1/6837 , C12Q2600/156 , C40B40/00 , G16B25/00 , G16B30/00 , C12Q2525/161 , C12Q2565/501 , C12Q2561/125
摘要： The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. The methods involve providing an array containing a large number (e.g. greater than 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location of each different probe is known. Nucleic acid samples (e.g. mRNA) from two or more samples are hybridized to the probe arrays and the pattern of hybridization is detected. Differences in the hybridization patterns between the samples indicates differences in expression of various genes between those samples. This invention also provides a method of end-labeling a nucleic acid. In one embodiment, the method involves providing a nucleic acid, providing a labeled oligonucleotide and then enzymatically ligating the oligonucleotide to the nucleic acid. Thus, for example, where the nucleic acid is an RNA, a labeled oligoribonucleotide can be ligated using an RNA ligase. In another embodiment, the end labeling can be accomplished by providing a nucleic acid, providing labeled nucleoside triphosphates, and attaching the nucleoside triphosphates to the nucleic acid using a terminal transferase.
摘要翻译： 本发明提供用于鉴定两个或多个样品之间的核酸丰度差异（例如，表达水平）的简化方法。 所述方法包括提供包含大量（例如大于1,000个）任意选择的不同寡核苷酸探针的阵列，其中每个不同探针的序列和位置是已知的。 来自两个或更多个样品的核酸样品（例如mRNA）与探针阵列杂交，并检测杂交模式。 样本之间的杂交模式的差异表明这些样品之间各种基因的表达差异。 本发明还提供了一种终止标记核酸的方法。 在一个实施方案中，该方法包括提供核酸，提供标记的寡核苷酸，然后将寡核苷酸酶连接到核酸上。 因此，例如，当核酸是RNA时，可以使用RNA连接酶连接标记的寡核糖核苷酸。 在另一个实施方案中，末端标记可以通过提供核酸，提供标记的核苷三磷酸和使用末端转移酶将核苷三磷酸与核酸连接来实现。
-
公开(公告)号：US20050158772A1
公开(公告)日：2005-07-21
申请号：US11021367
申请日：2004-12-23
申请人： David Lockhart , Mark Chee , Kevin Gunderson , Lai Chaoqiang , Lisa Wodicka , Maureen Cronin , Danny Lee , Huu Tran , Hajime Matsuzaki , Glenn McGall , Anthony Barone
发明人： David Lockhart , Mark Chee , Kevin Gunderson , Lai Chaoqiang , Lisa Wodicka , Maureen Cronin , Danny Lee , Huu Tran , Hajime Matsuzaki , Glenn McGall , Anthony Barone
CPC分类号： C07H19/052 , C07H19/12 , C07H21/00 , C12Q1/6809 , C12Q1/6837 , C12Q2600/156 , C40B40/00 , G16B25/00 , G16B30/00 , C12Q2525/161 , C12Q2565/501 , C12Q2561/125
摘要： The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. The methods involve providing an array containing a large number (e.g. greater than 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location of each different probe is known. Nucleic acid samples (e.g. mRNA) from two or more samples are hybridized to the probe arrays and the pattern of hybridization is detected. Differences in the hybridization patterns between the samples indicates differences in expression of various genes between those samples. This invention also provides a method of end-labeling a nucleic acid. In one embodiment, the method involves providing a nucleic acid, providing a labeled oligonucleotide and then enzymatically ligating the oligonucleotide to the nucleic acid. Thus, for example, where the nucleic acid is an RNA, a labeled oligoribonucleotide can be ligated using an RNA ligase. In another embodiment, the end labeling can be accomplished by providing a nucleic acid, providing labeled nucleoside triphosphates, and attaching the nucleoside triphosphates to the nucleic acid using a terminal transferase.
-
公开(公告)号：US20080176229A1
公开(公告)日：2008-07-24
申请号：US11755697
申请日：2007-05-30
申请人： David Agus , Steven Shak , Maureen Cronin , Joffre Baker
发明人： David Agus , Steven Shak , Maureen Cronin , Joffre Baker
IPC分类号： C12Q1/68 , G01N33/574 , C40B40/08 , C12P19/34
CPC分类号： C12Q1/6886 , C12Q2600/106 , C12Q2600/158
摘要： The present invention concerns prognostic markers associated with cancer. In particular, the invention concerns prognostic methods based on the molecular characterization of gene expression in paraffin-embedded, fixed samples of cancer tissue, which allow a physician to predict whether a patient is likely to respond well to treatment with an EGFR inhibitor.
摘要翻译： 本发明涉及与癌症相关的预后标志物。 特别地，本发明涉及基于石蜡包埋的癌症组织固定样品中基因表达的分子表征的预后方法，其允许医师预测患者是否可能对EGFR抑制剂治疗反应良好。
-
公开(公告)号：US07312034B2
公开(公告)日：2007-12-25
申请号：US10811248
申请日：2004-03-26
申请人： Carmen Virgos , Maureen Cronin
发明人： Carmen Virgos , Maureen Cronin
CPC分类号： C12Q1/6816 , C12Q2537/143
摘要： The invention provides universal e-tag primers and methods for their use in the sequence specific detection and multiplexed analysis of known, selected target nucleic acid sequences. The universal e-tag primers have sequence specific and universal components for use in e-tag probe-mediated analysis of target nucleic acids.
摘要翻译： 本发明提供通用电子标签引物及其用于已知选择的靶核酸序列的特异性检测和多重分析的方法。 通用电子标签引物具有序列特异性和通用组分，用于e-标签探针介导的目标核酸分析。
-
公开(公告)号：US20070141589A1
公开(公告)日：2007-06-21
申请号：US11450963
申请日：2006-06-09
申请人： Joffre Baker , Maureen Cronin , Michael Kiefer , Steve Shak , Michael Walker
发明人： Joffre Baker , Maureen Cronin , Michael Kiefer , Steve Shak , Michael Walker
IPC分类号： C12Q1/68
CPC分类号： G01N33/57484 , C12N15/1003 , C12Q1/6886 , C12Q2600/106 , C12Q2600/118 , C12Q2600/158 , C12Q2600/16 , G01N33/57415
摘要： The invention concerns sensitive methods to measure mRNA levels in biopsied tumor tissues, including archived paraffin-embedded biopsy material. The invention also concerns breast cancer gene sets important in the diagnosis and treatment of breast cancer, and methods for assigning the most optimal treatment options to breast cancer patient based upon knowledge derived from gene expression studies.
-
公开(公告)号：US20070141587A1
公开(公告)日：2007-06-21
申请号：US11450896
申请日：2006-06-09
申请人： Joffre Baker , Maureen Cronin , Michael Kiefer , Steve Shak , Michael Walker
发明人： Joffre Baker , Maureen Cronin , Michael Kiefer , Steve Shak , Michael Walker
IPC分类号： C12Q1/68
CPC分类号： G01N33/57484 , C12N15/1003 , C12Q1/6886 , C12Q2600/106 , C12Q2600/118 , C12Q2600/158 , C12Q2600/16 , G01N33/57415
摘要： The invention concerns sensitive methods to measure mRNA levels in biopsied tumor tissues, including archived paraffin-embedded biopsy material. The invention also concerns breast cancer gene sets important in the diagnosis and treatment of breast cancer, and methods for assigning the most optimal treatment options to breast cancer patient based upon knowledge derived from gene expression studies.
摘要翻译： 本发明涉及测量活检肿瘤组织中的mRNA水平的敏感方法，包括归档石蜡包埋的活检材料。 本发明还涉及在乳腺癌诊断和治疗中重要的乳腺癌基因集，以及基于从基因表达研究获得的知识为乳腺癌患者分配最佳治疗选择的方法。
-
公开(公告)号：US20070059737A1
公开(公告)日：2007-03-15
申请号：US11450962
申请日：2006-06-09
申请人： Joffre Baker , Maureen Cronin , Michael Kiefer , Steve Shak , Michael Walker
发明人： Joffre Baker , Maureen Cronin , Michael Kiefer , Steve Shak , Michael Walker
IPC分类号： C12Q1/68
CPC分类号： G01N33/57484 , C12N15/1003 , C12Q1/6886 , C12Q2600/106 , C12Q2600/118 , C12Q2600/158 , C12Q2600/16 , G01N33/57415
摘要： The invention concerns sensitive methods to measure mRNA levels in biopsied tumor tissues, including archived paraffin-embedded biopsy material. Th invention also concerns breast cancer gene sets important in the diagnosis and treatment of breast cancer, and methods for assigning the most optimal treatment options to breast cancer patient based upon knowledge derived from gene expression studies.
摘要翻译： 本发明涉及测量活检肿瘤组织中的mRNA水平的敏感方法，包括归档石蜡包埋的活检材料。 本发明还涉及在乳腺癌的诊断和治疗中重要的乳腺癌基因集，以及基于来自基因表达研究的知识为乳腺癌患者分配最佳治疗选择的方法。
-
-
-
-
-
-
-
-
-
搜索结果
17 条记录 (耗时 0.043 秒)
