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18 条记录 (耗时 0.041 秒)

    Nucleic acid affinity columns
    5.
    发明申请
    Nucleic acid affinity columns 审中-公开
    核酸亲和柱

    公开(公告)号：US20060204991A1

    公开(公告)日：2006-09-14

    申请号：US11360146

    申请日：2006-02-22

    申请人： Robert Lipshutz ,  MacDonald Morris ,  Mark Chee ,  Thomas Gingeras

    发明人： Robert Lipshutz ,  MacDonald Morris ,  Mark Chee ,  Thomas Gingeras

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6806 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q1/6809 ,  C12Q1/6837 ,  C40B40/06 ,  C12Q2565/107 ,  C12Q2525/155

    摘要： This invention provides nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample Methods of producing such affinity matrices are also provided In general the methods involve the steps of a) providing a nucleic acid amplification template array comprising a surface to which are attached at least 50 oligonucleotides having different nucleic acid sequences, and wherein each different oligonucleotide is localized in a predetermined region of said surface, the density of said oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and all of said different oligonucleotides have an identical terminal 3′ nucleic acid sequence and an identical terminal 5′ nucleic acid sequence b) amplifying said multiplicity of oligonucleotides to provide a pool of amplified nucleic acids, and c) attaching the pool of nucleic acids to a solid support

    摘要翻译： 本发明提供了携带大量不同核酸亲和配体的核酸亲和基质，允许从样品中同时选择和除去大量预选择的核酸。还提供了产生这种亲和基质的方法一般来说，该方法涉及 步骤a）提供核酸扩增模板阵列，其包含与至少50个具有不同核酸序列的寡核苷酸连接的表面，并且其中每个不同的寡核苷酸定位在所述表面的预定区域中，所述寡核苷酸的密度较大 每1cm 2超过约60个不同的寡核苷酸，并且所有所述不同的寡核苷酸具有相同的末端3'核酸序列和相同的末端5'核酸序列b）扩增所述多重寡核苷酸以提供 一组扩增的核酸，以及c）附着核酸池 酸固体支持物

    Analysis of methylation status using nucleic acid arrays
    8.
    发明申请
    Analysis of methylation status using nucleic acid arrays 审中-公开
    使用核酸阵列分析甲基化状态

    公开(公告)号：US20050196792A1

    公开(公告)日：2005-09-08

    申请号：US11058566

    申请日：2005-02-14

    申请人： Stephen Fodor ,  Robert Lipshutz ,  Shivani Nautiyal

    发明人： Stephen Fodor ,  Robert Lipshutz ,  Shivani Nautiyal

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2521/331 ,  C12Q2565/501 ,  C12Q2533/101 ,  C12Q2525/179 ,  C12Q2521/125 ,  C12Q2531/119

    摘要： Methods for amplifying a nucleic acid sample while preserving the methylation status of cytosines are disclosed. In some aspects the amplified methylated sample is modified by methylation sensitive modification and analyzed by hybridization to an array to identify cytosines that were methylated in the starting material and cytosines that were not methylated in the starting material. Methods for detecting methylation status are also disclosed. In one embodiment a DNA methyltransferase activity is included in the amplification reaction and this activity methylates the newly synthesized DNA using the methylated genomic template strand as a guide.

    摘要翻译： 公开了扩增核酸样品同时保持胞嘧啶甲基化状态的方法。 在一些方面，通过甲基化敏感性修饰来修饰扩增的甲基化样品，并通过与阵列杂交来分析，以鉴定起始原料中甲基化的胞嘧啶和起始材料中未被甲基化的胞嘧啶。 还公开了检测甲基化状态的方法。 在一个实施方案中，DNA甲基转移酶活性包括在扩增反应中，并且该活性使用甲基化基因组模板链作为指导对新合成的DNA进行甲基化。