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41 条记录 (耗时 0.075 秒)

    System and method for determining copies-per-unit-volume using PCR and flow control of droplets
    21.
    发明授权
    System and method for determining copies-per-unit-volume using PCR and flow control of droplets 有权
    使用PCR和液滴的流量控制来确定每单位体积份数的系统和方法

    公开(公告)号：US09322055B2

    公开(公告)日：2016-04-26

    申请号：US14009304

    申请日：2012-03-30

    申请人： Gordon A. Janaway ,  Mark Andersen ,  Kornelija Zgonc ,  Michael Pallas ,  Marcin Sikora ,  Casey McFarland ,  Ferrier N. Le ,  Haopeng Wang ,  Jian Gong ,  Gothami Padmabandu

    发明人： Gordon A. Janaway ,  Mark Andersen ,  Kornelija Zgonc ,  Michael Pallas ,  Marcin Sikora ,  Casey McFarland ,  Ferrier N. Le ,  Haopeng Wang ,  Jian Gong ,  Gothami Padmabandu

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6886 ,  C12Q1/686 ,  C12Q2600/158 ,  C12Q2600/178 ,  G01N21/6486 ,  C12Q2537/16 ,  C12Q2563/143 ,  C12Q2563/159 ,  C12Q2565/626

    摘要： Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

    摘要翻译： 提供了用于定量样品中靶核酸的方法和系统。 该方法包括形成多个离散的样本部分。 多个离散样品部分中的每一个包含样品的一部分和反应混合物。 该方法还包括放大多个离散样本部分以形成多个离散处理的样本部分。 至少一个离散加工的样品部分含有核酸扩增反应产物。 从所述多个离散处理样品部分中的至少一个检测荧光信号以确定所述至少一种靶核酸的存在。 该方法还包括确定多个多个离散处理的样本部分的相应体积，以及估计样品中至少一个靶核酸的每单位体积的拷贝数。 估计每单位体积的拷贝数是基于确定为在其中含有至少一种靶核酸的离散加工样品部分的数量。

    Pet toy
    25.
    外观设计
    Pet toy 有权

    公开(公告)号：USD984759S1

    公开(公告)日：2023-04-25

    申请号：US29779513

    申请日：2021-04-20

    申请人： Jian Gong

    设计人： Jian Gong

    Method and System to Separate and Measure 37Ar Quickly
    26.
    发明申请
    Method and System to Separate and Measure 37Ar Quickly 失效
    快速分离和测量37Ar的方法和系统

    公开(公告)号：US20080028830A1

    公开(公告)日：2008-02-07

    申请号：US10583579

    申请日：2004-12-20

    申请人： Wei Li ,  Hongxia Wang ,  Rongliang Duan ,  Zhishang Bian ,  Yongchun Xiang ,  Meiying He ,  Jian Gong

    发明人： Wei Li ,  Hongxia Wang ,  Rongliang Duan ,  Zhishang Bian ,  Yongchun Xiang ,  Meiying He ,  Jian Gong

    IPC分类号： G01N30/02 ,  G01N25/18

    CPC分类号： G01T7/00

    摘要： The present invention relates to the method and equipment for inspecting nuclear experimental locales, and particularly relates to the method and system for separating and measuring 37Ar quickly. The method of the invention comprises the steps of sampling, eliminating impurities, separating, purifying, measuring the sum of Ar, collecting Ar, measuring the activity of 37Ar, etc. The control unit in the system of the invention connects respectively to a sampling unit, a separating-purifying unit, and a radioactivity measuring unit which are connected in turn, and a computer and the software of the control unit are in charge of the automatic operation, measurement and data-collection of the whole system. The method and system for quickly separating and measuring 37Ar of the patent can meet the requirements of locales inspection for Comprehensive Test Ban Treaty (CTBT), the sensitivity of measuring 37Ar is high, so do the production and purity of Ar. The system can also be operated on vehicle, which is flexible and has good work efficiency.

    摘要翻译： 本发明涉及用于核实验区的检查方法和设备，特别涉及用于快速分离测量的方法和系统。 本发明的方法包括以下步骤：对杂质进行取样，除去，净化，测量Ar的总和，收集Ar，测量Ar的活度等的步骤。系统中的控制单元 本发明分别连接到依次连接的采样单元，分离净化单元和放射性测量单元，计算机和控制单元的软件负责自动操作，测量和数据采集 整个系统。 专利的快速分离和测量方法和系统可以满足“全面禁止核试验条约”（CTBT）的地区检查要求，测量“37”的灵敏度 是高的，所以生产和纯度的Ar。 该系统也可以在车辆上运行，灵活，工作效率好。

    SMALL OBJECT MOVING ON PRINTED CIRCUIT BOARD
    27.
    发明申请
    SMALL OBJECT MOVING ON PRINTED CIRCUIT BOARD 审中-公开
    打印电路板上的小物体移动

    公开(公告)号：US20070023292A1

    公开(公告)日：2007-02-01

    申请号：US11460188

    申请日：2006-07-26

    申请人： Chang-Jin Kim ,  Jian Gong

    发明人： Chang-Jin Kim ,  Jian Gong

    IPC分类号： B03C5/02

    CPC分类号： F04B19/006 ,  B01F13/0076 ,  B01L3/502792 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427

    摘要： A printed circuit board based digital or droplet microfluidic system and method for producing such microfluidic system are disclosed. The digital microfluidic device comprises a printed circuit board having a substrate and a plurality of electrode pads disposed on the top surface of the substrate in a rectangular array. A via extends from each electrode pad through the substrate to other locations on the substrate . A dielectric layer is disposed on the electrode pads. Droplets may be manipulated using electrowetting principles and others by applying a voltage to the desired electrodes. Each electrode pad can be controlled directly and independently from the other electrode pads to modify the surface wettability of the dielectric layer in the vicinity of the electrode pad by applying a voltage to the desired electrode pad(s). In this way, droplets may be formed, moved, mixed, and/or divided or other small objects manipulated while in air or immersed in a liquid on the dielectric surface.

    摘要翻译： 公开了一种用于生产这种微流体系统的基于印刷电路板的数字或液滴微流体系统和方法。 数字微流体装置包括具有衬底的印刷电路板和以矩形阵列布置在衬底的顶表面上的多个电极焊盘。 通孔从每个电极焊盘穿过衬底延伸到衬底上的其它位置。 电介质层设置在电极焊盘上。 可以使用电润湿原理和其它步骤通过向期望的电极施加电压来操纵液滴。 每个电极焊盘可以直接和独立于其他电极焊盘进行控制，以通过向期望的电极焊盘施加电压来改变电极焊盘附近的电介质层的表面润湿性。 以这种方式，液滴可以被形成，移动，混合和/或分割，或者在空气中被操纵的其它小物体或浸在电介质表面上的液体中。

    Circuit simulation using precision-space concept
    28.
    发明申请
    Circuit simulation using precision-space concept 审中-公开
    电路仿真采用精密空间概念

    公开(公告)号：US20070005325A1

    公开(公告)日：2007-01-04

    申请号：US11174124

    申请日：2005-06-30

    申请人： Jian Gong ,  Changhong Dai

    发明人： Jian Gong ,  Changhong Dai

    IPC分类号： G06F17/50

    CPC分类号： G06F17/504

    摘要： An arrangement is provided for using a precision space (“p-space”) to represent s-parameters when analyzing/simulating a circuit/network. The p-space is one dimensional with a value range corresponding to the value range of s-parameters. The p-space is divided into multiple slots where the number of slots may depend on the permissible precision of s-parameter values. A mapping relationship between s-parameters and p-space slots may be obtained by partitioning an s-parameter matrix. Based on the mapping relationship, p-space representations of original s-parameters may be generated through a forward projection process from s-parameters in a two-dimensional matrix to the one-dimensional p-space. During the simulation process, a p-space representation may be projected back to original s-parameters in a matrix based on the mapping relationship.

    摘要翻译： 提供了一种在分析/模拟电路/网络时使用精密空间（“p空间”）表示s参数的布置。 p空间是与s参数的值范围对应的值范围的一维。 p空间被分成多个槽，其中槽数可以取决于s参数值的允许精度。 可以通过划分s参数矩阵来获得s参数和p空间时隙之间的映射关系。 基于映射关系，可以通过从二维矩阵中的s参数到一维p空间的前向投影处理来生成原始s参数的p空间表示。 在仿真过程中，可以基于映射关系将p空间表示投影回矩阵中的原始s参数。

    Method and apparatus for simulating circuits using s-parameters
    29.
    发明申请
    Method and apparatus for simulating circuits using s-parameters 审中-公开
    使用s参数模拟电路的方法和装置

    公开(公告)号：US20070005324A1

    公开(公告)日：2007-01-04

    申请号：US11174071

    申请日：2005-06-30

    申请人： Jian Gong ,  Changhong Dai

    发明人： Jian Gong ,  Changhong Dai

    IPC分类号： G06F17/50

    CPC分类号： G06F17/5036

    摘要： An arrangement is provided for using s-parameters to obtain characteristics of a device under test (“DUT”) between a number of selected observation locations. The DUT may be represented by a network of models such as lumped device models and transmission line models. S-parameters between the selected nodes may be measured based on the DUT representation at a plurality of frequency points. The measured s-parameters may be converted into their precision space (“p-space”) representations, which may then be submitted to a simulator to obtain the DUT characteristics at the selected observation nodes.

    摘要翻译： 提供了一种用于使用s参数来获得多个所选观察位置之间的待测设备（“DUT”）的特性的装置。 DUT可以由诸如集总装置模型和传输线模型的模型网络来表示。 可以基于多个频点处的DUT表示来测量所选节点之间的S参数。 测量的s参数可以转换成它们的精确空间（“p空间”）表示，然后可以将其提交给模拟器以在所选择的观测节点处获得DUT特性。