公开(公告)号：US20220340925A1

公开(公告)日：2022-10-27

申请号：US13873092

申请日：2013-04-29

Applicant: Monsanto Technology LLC

Inventor： Yurong Chen ,  Brian J. Martinell ,  Anatoly Rivlin ,  Yuechun Wan ,  Edward J. Williams ,  Xudong Ye ,  Ashok Shrawat

IPC: C12N15/82

Abstract: The present invention provides methods for the production of viable explants from mature corn seeds, wherein the explant comprises the apical portion of the embryo axis of the corn seed. The present invention also relates to methods for producing such explants and for transforming the explants with a heterologous DNA.

公开(公告)号：US20210246459A1

公开(公告)日：2021-08-12

申请号：US17118539

申请日：2020-12-10

Applicant: Monsanto Technology LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  C12N5/00 ,  A01H4/00 ,  C12N5/04 ,  G01N33/00 ,  C12N9/10

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US20170314032A1

公开(公告)日：2017-11-02

申请号：US15619192

申请日：2017-06-09

Applicant: Monsanto Technology LLC

Inventor： Vladimir Sidorov ,  Jeffrey M. Staub ,  Yuechun Wan ,  Guangning Ye

IPC: C12N15/82

CPC classification number: C12N15/8214

Abstract: A method is provided for transforming monocotyledonous plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of monocot plant tissue with heterologous DNA constructs. The invention also provides for monocot cells in which the plastids contain heterologous DNA constructs.

公开(公告)号：US09701973B2

公开(公告)日：2017-07-11

申请号：US14988970

申请日：2016-01-06

Applicant: Monsanto Technology LLC

Inventor： Vladimir Sidorov ,  Jeffrey M. Staub ,  Yuechun Wan ,  Guangning Ye

IPC: C12N15/82

CPC classification number: C12N15/8214

Abstract: A method is provided for transforming monocotyledonous plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of monocot plant tissue with heterologous DNA constructs. The invention also provides for monocot cells in which the plastids contain heterologous DNA constructs.

公开(公告)号：US20130185830A1

公开(公告)日：2013-07-18

申请号：US13727575

申请日：2012-12-26

Applicant: Monsanto Technology LLC

Inventor： Erik Dersch ,  Richard J. Heinzen ,  Brian J. Martinell ,  Anatoly Rivlin ,  Yuechun Wan ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.