公开(公告)号：US20190002856A1

公开(公告)日：2019-01-03

申请号：US16135407

申请日：2018-09-19

Applicant: NOVOZYMES A/S

Inventor： Esben Peter Friis ,  Leonardo De Maria ,  Jesper Vind ,  Thomas A. Poulsen ,  Allan Svendsen ,  Steffen Danielsen ,  Rolf T. Lenhard ,  Henrik Friis-Madsen ,  Lars K. Skov

IPC: C12N9/34 ,  C12N9/30 ,  C12P5/00 ,  C12P7/46 ,  C12P7/48 ,  C12P37/00 ,  C12P13/20 ,  C12P7/54 ,  C12P7/06 ,  C12P5/02 ,  C12P19/20 ,  C12P7/56

Abstract: The present invention relates to glucoamylase variants having improved thermostability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US09951299B2

公开(公告)日：2018-04-24

申请号：US15101275

申请日：2014-12-10

Applicant: Novozymes A/S

Inventor： Leonardo De Maria ,  Rakhi Saikia ,  Santhosh Vasu Mepadam ,  Ting Sun

IPC: C12N9/18 ,  C11D3/386 ,  C11D11/00 ,  D06M16/00 ,  D06M101/32

CPC classification number: C11D3/38636 ,  C11D11/0017 ,  C12N9/18 ,  C12Y301/01074 ,  D06M16/003 ,  D06M2101/32 ,  D06M2200/35

Abstract: The present invention relates to variants with cutinase activity of a parent cutinase, comprising an alteration at one or more (e.g. several) positions corresponding to positions: 36, 37, 40, 78, 90, 114, 150, 196, 216, or 217 of SEQ ID NO: 2, wherein the alteration is a substitution for positions 40, 78, 90, 114, 150, 196 and 216, and a deletion for positions 36, 37 and 217, and wherein the variant has at least 75% but less than 100% sequence identity to the mature polypeptide of SEQ ID NO: 2.The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and method for obtaining and methods of producing the variants. It also relates to compositions comprising the variant, and to methods for using the variant.

公开(公告)号：US09663804B2

公开(公告)日：2017-05-30

申请号：US14412803

申请日：2013-07-18

Applicant: Novozymes A/S

Inventor： Weijian Lai ,  Ting Sun ,  Leonardo De Maria

IPC: D06M16/00 ,  D06M101/32 ,  C12P7/62 ,  C12N9/24

CPC classification number: C12P7/62 ,  C12N9/24 ,  C12Y301/01074 ,  D06M16/003 ,  D06M2101/32

Abstract: The present invention relates to the use of glycosyl hydrolase family 61 polypeptides in the presence of cutinases for polyester textile manufacture as well as a textile composition comprising glycosyl hydrolase family 61 polypeptides and cutinases.

公开(公告)号：US09540624B2

公开(公告)日：2017-01-10

申请号：US15139050

申请日：2016-04-26

Applicant: NOVOZYMES A/S

Inventor： Esben Peter Friis ,  Leonardo De Maria ,  Jesper Vind ,  Thomas A. Poulsen ,  Allan Svendsen ,  Steffen Danielsen ,  Rolf T. Lenhard ,  Henrik Friis-Madsen ,  Lars K. Skov

IPC: C12N9/24 ,  C12N9/34 ,  C12P19/20 ,  C12P5/02 ,  C12P7/06 ,  C12P7/54 ,  C12P13/20 ,  C12P37/00

CPC classification number: C12N9/2428 ,  C12N9/242 ,  C12P5/002 ,  C12P5/02 ,  C12P7/06 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P13/20 ,  C12P19/20 ,  C12P37/00 ,  C12Y302/01001 ,  C12Y302/01003 ,  Y02E50/17

Abstract: The present invention relates to glucoamylase variants having improved thermostability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract translation: 本发明涉及具有改善的热稳定性的葡糖淀粉酶变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。

公开(公告)号：US09353362B2

公开(公告)日：2016-05-31

申请号：US14351469

申请日：2012-10-11

Applicant: NOVOZYMES A/S

Inventor： Esben Peter Friis ,  Leonardo De Maria ,  Jesper Vind ,  Thomas A. Poulsen ,  Allan Svendsen ,  Steffen Danielsen ,  Rolf T. Lenhard ,  Henrik Friis-Madsen ,  Lars K. Skov

IPC: C12N9/24 ,  C12N9/34 ,  C12P19/20

CPC classification number: C12N9/2428 ,  C12N9/242 ,  C12P5/002 ,  C12P5/02 ,  C12P7/06 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P13/20 ,  C12P19/20 ,  C12P37/00 ,  C12Y302/01001 ,  C12Y302/01003 ,  Y02E50/17

Abstract: The present invention relates to glucoamylase variants having improved thermostability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract translation: 本发明涉及具有改善的热稳定性的葡糖淀粉酶变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。

公开(公告)号：US20150152397A1

公开(公告)日：2015-06-04

申请号：US14403949

申请日：2013-05-30

Applicant: NOVOZYMES A/S

Inventor： Bjarne Gram Hansen ,  Steffen Danielsen ,  Lars Kobberoee Skov ,  Leonardo De Maria ,  Julie Bille Rannes

IPC: C12N9/16 ,  A62D3/30

CPC classification number: C12N9/16 ,  A62D3/02 ,  A62D3/30 ,  A62D2101/02 ,  A62D2101/04 ,  A62D2101/26 ,  C12Y301/08002

Abstract: The present invention relates to isolated polypeptides having organophosphorous hydrolase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract translation: 本发明涉及具有有机磷水解酶活性的分离的多肽和编码多肽的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

公开(公告)号：US20150024084A1

公开(公告)日：2015-01-22

申请号：US14504517

申请日：2014-10-02

Applicant: NOVOZYMES A/S

Inventor： Leonardo De Maria ,  Carsten Andersen ,  Lars Kobberoee Skov ,  Mikael Blom Soerensen

IPC: A23K1/165 ,  C12N9/16

CPC classification number: C12N9/16 ,  A23K20/189 ,  C12Y301/03

Abstract: The present invention relates to a phytase which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration as compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

Abstract translation: 本发明涉及一种植酸酶，其与来自柠檬酸杆菌的植酸酶具有至少74％的同一性，并且与该植酸酶相比包含至少一个改变。 这些植酸酶变体具有修饰，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

公开(公告)号：US20150023945A1

公开(公告)日：2015-01-22

申请号：US14500419

申请日：2014-09-29

Applicant: Novozymes A/S

Inventor： Mikkel Klausen ,  Marie Allesen Holm ,  Leonardo De Maria ,  Michael Skjoet

IPC: C12N9/36 ,  C11D3/386 ,  A23K1/165 ,  A61K38/47

CPC classification number: C12N9/2462 ,  A23K20/189 ,  A61K38/47 ,  C11D3/38636 ,  C12Y302/01017

Abstract: The present invention is directed to lysozyme variants and nucleotide sequences encoding same. The lysozyme variants have antimicrobial and/or lysozyme activity and comprise an alteration of an amino acid sequence at one or more positions. The present invention is also directed to methods for producing and of using the Opisthocomus hoazin lysozyme and the lysozyme variants of the present invention as antimicrobial agents.

Abstract translation: 本发明涉及溶菌酶变体和编码其的核苷酸序列。 溶菌酶变体具有抗微生物和/或溶菌酶活性，并且包括在一个或多个位置上氨基酸序列的改变。 本发明还涉及用于制造和使用本发明的眼药水溶菌酶和溶菌酶变体作为抗微生物剂的方法。

公开(公告)号：US20140212938A1

公开(公告)日：2014-07-31

申请号：US14242721

申请日：2014-04-01

Applicant: Novozymes A/S ,  Novozymes, Inc.

Inventor： Monica Tassone ,  Leonardo De Maria

IPC: C12N9/04 ,  C12P7/42

CPC classification number: C12N9/0006 ,  C12P7/42 ,  C12P7/52 ,  C12Y101/01059 ,  C12Y101/01276 ,  C12Y101/01298 ,  Y02P20/52

Abstract: The present invention relates to 3-hydroxypropionate dehydrogenase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract translation: 本发明涉及3-羟基丙酸酯脱氢酶变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。

公开(公告)号：US20130224332A1

公开(公告)日：2013-08-29

申请号：US13874954

申请日：2013-05-01

Applicant: Novozymes A/S

Inventor： Leonardo De Maria ,  Carsten Andersen ,  Lars Kobberoee Skov ,  Mikael Blom Soerensen

IPC: C12N9/16 ,  A23K1/165

CPC classification number: C12N9/16 ,  A23K20/189 ,  C12Y301/03

Abstract: The present invention relates to a phytase which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration as compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.

Abstract translation: 本发明涉及一种植酸酶，其与来自柠檬酸杆菌的植酸酶具有至少74％的同一性，并且与该植酸酶相比包含至少一个改变。 这些植酸酶变体具有修饰，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如， 在动物饲料和动物饲料添加剂中。