Immobilization of enzymes on particulate porous carriers for use in organic mediums
    23.
    发明授权
    Immobilization of enzymes on particulate porous carriers for use in organic mediums 有权
    在用于有机介质的颗粒状多孔载体上固定酶

    公开(公告)号:US06582942B1

    公开(公告)日:2003-06-24

    申请号:US09649942

    申请日:2000-08-29

    IPC分类号: C12P764

    CPC分类号: C12N11/08 C12N11/00 C12N11/14

    摘要: An immobilized enzyme is prepared for use in organic mediums essentially devoid of free water. An enzyme-containing liquid medium is contacted with a particulate porous carrier which preferably has a particle size of 200-1000 &mgr;m and a surface area of 20-1000 m2/g to adsorb the enzyme on the carrier, and volatile components of the liquid medium contained by the carrier are removed. The carrier may have a hydrophilic surface, and an amount of liquid medium is used to prevent agglomeration of the carrier. Alternatively, the carrier has a hydrophobic surface, and the addition of a hygroscopic substance suppresses agglomeration of the carrier by absorbing excess liquid. The hygroscopic substance may be removed during the removal of volatile components. Contacting of the enzyme-containing liquid and carrier can be in a fluidized bed where immobilization and removing volatile components are conducted simultaneously, or contacting can be in mixer followed by removing volatile components in a fluidized bed. The enzyme-containing liquid may be atomized onto the carrier in the fluidized bed or in the mixer. Enzymes immobilized include lipase, and the immobilized lipase can be used in trans-esterification reactions.

    摘要翻译: 制备用于基本上不含游离水的有机介质中的固定化酶。 将含酶的液体培养基与粒径为200-1000μm,表面积为20-1000m 2 / g的颗粒状多孔质载体接触以将酶吸附在载体上,并且液体介质的挥发性成分 由载体包含的被移除。 载体可以具有亲水表面,并且使用一定量的液体介质来防止载体的聚集。 或者,载体具有疏水性表面,并且加入吸湿性物质通过吸收多余的液体来抑制载体的聚集。 在去除挥发性组分期间可以除去吸湿物质。 含酶液体和载体的接触可以在流化床中,其中同时进行固定和除去挥发性组分,或者可以在混合器中接触,然后在流化床中除去挥发性组分。 含酶液体可以在流化床或混合器中雾化到载体上。 固定的酶包括脂肪酶,并且固定的脂肪酶可以用于酯交换反应。

    Enzymatic method for textile dyeing
    24.
    发明授权
    Enzymatic method for textile dyeing 失效
    纺织染色的酶法

    公开(公告)号:US6036729A

    公开(公告)日:2000-03-14

    申请号:US770760

    申请日:1996-12-19

    摘要: The present invention relates to methods of dyeing a material, comprising (a) soaking the material in an aqueous solution which comprises one or more mono-, di- or polycyclic aromatic or heteroaromatic compounds; and (b) treating the soaked material in an aqueous solution with (i) a hydrogen peroxide source and an enzyme exhibiting peroxidase activity or (ii) an enzyme exibiting oxidase activity on the one or more aromatic or heteroaromatic compounds; wherein the material is a fabric, yarn, fiber, garment or film made of fur, hide, leather, silk or wool.

    摘要翻译: 本发明涉及染色材料的方法,其包括(a)将材料浸泡在包含一种或多种单 - ,二 - 或多环芳族或杂芳族化合物的水溶液中; 和(b)用(i)过氧化氢源和显示过氧化物酶活性的酶处理所述浸渍物质,或(ii)一种或多种芳香族或杂芳族化合物的酶促除氧化酶活性; 其中所述材料是由毛皮,皮革,皮革,丝绸或羊毛制成的织物,纱线,纤维,衣服或薄膜。

    Dye transfer inhibition
    26.
    发明授权
    Dye transfer inhibition 失效
    染料转移抑制

    公开(公告)号:US5855621A

    公开(公告)日:1999-01-05

    申请号:US448292

    申请日:1995-05-23

    摘要: The transfer of a textile dye from a dyed fabric to another fabric during washing or rinsing is inhibited by adding an enzyme exhibiting peroxidase activity or an enzyme exhibiting a suitable oxidase activity to the wash liquor in which said fabrics are washed and/or rinsed. Peroxidase is produced extracellularly by some strains of Bacillus pumilus. The novel peroxidase preparation from B. pumilus is a microperoxidase, i.e. it contains hemopeptide as an active component. The preparation has improved stability at high temperature, at high pH and at high concentrations of hydrogen peroxide. It can be produced without undesired catalase activity.

    摘要翻译: 在洗涤或漂洗过程中,向洗涤液中加入显示过氧化物酶活性的酶或表现出合适的氧化酶活性的酶,将纺织染料从染色织物转移到另一种织物。 过氧化物酶由一些短小芽孢杆菌菌株细胞外产生。 来自短小芽孢杆菌的新型过氧化物酶制剂是微过氧化物酶,即它含有血肽作为活性成分。 该制剂改善了高温下,高pH和高浓度过氧化氢的稳定性。 可以产生没有不需要的过氧化氢酶活性。

    Bleaching detergent composition containing acylated sugar bleach
activators
    28.
    发明授权
    Bleaching detergent composition containing acylated sugar bleach activators 失效
    含有酰化糖漂白活化剂的漂白洗涤剂组合物

    公开(公告)号:US5431849A

    公开(公告)日:1995-07-11

    申请号:US720538

    申请日:1991-06-19

    CPC分类号: C11D1/662 C11D3/3912

    摘要: Esters of monosaccharides and their lower alkyl glycosides are effective both as suffactants and as bleach activators (peracid precursors). The compounds are non-toxic and biodegradable. They act as nonionic suffactants and are effective in soil removal from textiles, e.g. of fatty soiling. In the presence of a hydrogen peroxide source, the sugar derivatives are perhydrolyzed during the washing process to form long-chain peracid. This enhances the bleaching effect, especially on hydrophobic stains.

    摘要翻译: PCT No.PCT / DK90 / 00022 Sec。 371日期1991年6月19日 102(e)日期1991年6月19日PCT提交1990年1月22日PCT公布。 出版物WO90 / 08182 日期:1990年7月26日。单糖和它们的低级烷基糖苷的酯类作为腋下和漂白活化剂(过酸前体)都是有效的。 这些化合物是无毒且可生物降解的。 它们作为非离子型人体,并且有效地从织物去除土壤,例如, 的脂肪污染。 在过氧化氢源的存在下,糖衍生物在洗涤过程中被过水解以形成长链过酸。 这增强了漂白效果,特别是在疏水污渍上。