公开(公告)号：US10227366B2

公开(公告)日：2019-03-12

申请号：US14610110

申请日：2015-01-30

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Robert Cysewski ,  Luisa de Cola ,  Jesus Miguel Fernandez Hernandez ,  Hans-Peter Josel ,  Elena Longhi ,  Gaston Hubertus Maria Vondenhoff

IPC: C07F15/00 ,  G01N33/533 ,  H01L51/00 ,  C09K11/06 ,  G01N33/58 ,  H01L51/50

Abstract: The present invention relates to novel bis-iridium-based Ir(III) complexes, labels manufactured using these complexes and a method for producing such complexes.

公开(公告)号：US20180364175A1

公开(公告)日：2018-12-20

申请号：US16111646

申请日：2018-08-24

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Herbert Andres ,  Michaela Windfuhr ,  Oliver Larbolette ,  Stefan Quint

IPC: G01N21/76 ,  C07F15/00 ,  G01N21/66

Abstract: The disclosure concerns methods for the detection of an analyte in a sample by electrochemiluminescence using new reagent compositions. New reagent compositions, reagent kits for measuring electrochemiluminscence (ECL) and electrochemiluminescence detection methods using the new reagent compositions are disclosed. In particular, the disclosure relates to the use of novel combinations of compounds which can be used in said measurements to provide improved assay performance.

公开(公告)号：US20180141935A1

公开(公告)日：2018-05-24

申请号：US15186580

申请日：2016-06-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: C07D409/04 ,  C12M1/42

CPC classification number: C07D409/04 ,  C07J51/00 ,  C12M35/04 ,  C12N5/0068 ,  C12N2533/20 ,  C12N2533/30 ,  G01N33/5005 ,  G01N33/54353

Abstract: The present invention relates to the use compounds comprising two or more hydrophobic domains and a hydrophilic domain comprising a polyethylene glycol (PEG) moiety for stabilization of a cell, and methods related thereto.

公开(公告)号：US20170146533A1

公开(公告)日：2017-05-25

申请号：US15186578

申请日：2016-06-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: G01N33/569 ,  C07D311/82 ,  G01N33/58 ,  C07J17/00 ,  C07D495/04 ,  C07D311/04 ,  C07J9/00

Abstract: The present invention relates to novel compounds comprising one or more hydrophobic domains and a hydrophilic domain comprising PEG moieties, useful for binding cells, as well as uses and compositions related thereto. The compounds are useful for immobilizing and/or stabilizing cells.

公开(公告)号：US09416150B2

公开(公告)日：2016-08-16

申请号：US14609649

申请日：2015-01-30

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Frank Bergmann ,  Robert Cysewski ,  Luisa de Cola ,  Sebastian Dziadek ,  Jesus Miguel Fernandez Hernandez ,  Hans-Peter Josel ,  Christoph Seidel

IPC: C07F15/00 ,  G01N33/58 ,  C09K11/06 ,  C09K11/07

CPC classification number: C07F15/0033 ,  C09K11/06 ,  C09K11/07 ,  C09K2211/1007 ,  C09K2211/1029 ,  C09K2211/14 ,  C09K2211/1408 ,  C09K2211/1416 ,  C09K2211/1425 ,  C09K2211/1441 ,  C09K2211/1466 ,  C09K2211/185 ,  G01N33/582

Abstract: The present invention relates to novel iridium-based Ir(III) luminescent complexes, conjugates comprising these complexes as a label and their application, e.g. in the electrochemiluminescence based detection of an analyte.

Abstract translation: 本发明涉及新型的基于铱的Ir（III）发光复合物，包含这些络合物作为标记的缀合物及其应用，例如， 在基于电化学发光的分析物检测中。

公开(公告)号：US09403859B2

公开(公告)日：2016-08-02

申请号：US14610372

申请日：2015-01-30

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Frank Bergmann ,  Robert Cysewski ,  Luisa de Cola ,  Sebastian Dziadek ,  Jesus Miguel Fernandez Hernandez ,  Hans-Peter Josel ,  Christoph Seidel

IPC: C07F15/00 ,  G01N33/58 ,  C09K11/06

CPC classification number: C07F15/0033 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1011 ,  C09K2211/1029 ,  C09K2211/14 ,  C09K2211/1408 ,  C09K2211/1416 ,  C09K2211/1425 ,  C09K2211/1441 ,  C09K2211/1466 ,  C09K2211/185 ,  G01N33/582

Abstract: The present invention relates to novel iridium-based Ir(III) luminescent complexes, conjugates comprising these complexes as a label and their application, e.g. in the electrochemiluminescence based detection of an analyte.

公开(公告)号：US08835637B2

公开(公告)日：2014-09-16

申请号：US13961401

申请日：2013-08-07

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Robert Cysewski ,  Luisa de Cola ,  Jesus Miguel Fernandez Hernandez ,  Hans-Peter Josel ,  Eloisa Lopez-Calle ,  Toralf Zarnt

IPC: C07F15/00 ,  G01N33/533

CPC classification number: C07F15/0033 ,  G01N21/76 ,  G01N33/533 ,  G01N33/582 ,  G01N2458/30

Abstract: Novel iridium-based Ir(III) luminescent complexes, conjugates comprising these complexes as a label and their application, for example in the electrochemiluminescence based detection of an analyte.

Abstract translation: 新型铱基Ir（III）发光复合物，包含这些络合物作为标记的缀合物及其应用，例如在基于电化学发光的检测分析物中。

公开(公告)号：US20130344489A1

公开(公告)日：2013-12-26

申请号：US13766297

申请日：2013-02-13

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Michael Gerg ,  Rupert Hermann ,  Rosa Isabel Saez Diaz

IPC: G01N1/30

CPC classification number: G01N1/30

Abstract: The present disclosure relates to novel bis-maleic anhydrides and to the surprising discovery that bis-maleic anhydride cross-linking agents can be used for preservation/fixation of a cell or tissue sample. Various bis-maleic anhydride cross-linking agent scan be used in methods requiring fixation of a cell or tissue sample. These reagents and methods are especially useful in procedures that require that the fixation agent be removed in order to facilitate analysis with other reagents. The inventive reagents and methods make it easier to reliably assay for various proteins, a nucleic acid and the like using analytical methods such as like immunohistochemistry, fluorescence in situ hybridization, RT-PCR, and the like.

Abstract translation: 本公开涉及新颖的双马来酸酐，以及令人惊奇的发现，即双马来酸酐交联剂可用于保存/固定细胞或组织样品。 各种双马来酸酐交联剂扫描用于需要固定细胞或组织样品的方法。 这些试剂和方法在需要除去固定剂以便于与其它试剂分析的方法中特别有用。 本发明的试剂和方法使得可以使用诸如免疫组织化学，荧光原位杂交，RT-PCR等的分析方法更容易地可靠地测定各种蛋白质，核酸等。

公开(公告)号：US20130023055A1

公开(公告)日：2013-01-24

申请号：US13630665

申请日：2012-09-28

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Hans-Joachim Kytzia ,  Matthias Muecke

IPC: G01N21/75

CPC classification number: G01N33/84 ,  C07C229/18

Abstract: Reagents and methods for determination of calcium within a sample. Specific embodiments include a reagent for determination of calcium comprising a mono-nitro substituted BAPTA-type chelator (BAPTA=1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid). Methods for accurate determination of calcium in a sample, such as a blood, whole blood, plasma or serum or any other aqueous liquid sample including cerebrospinal fluid, lymph, salivary juice or urine. Embodiments are also useful for clinical diagnoses.

Abstract translation: 用于测定样品中钙的试剂和方法。 具体实施方案包括用于测定钙的试剂，其包含单硝基取代的BAPTA型螯合剂（BAPTA = 1,2-双（2-氨基苯氧基）乙烷-N，N，N'，N'-四乙酸）。 用于精确测定样品（例如血液，全血，血浆或血清）或任何其它含水液体样品（包括脑脊液，淋巴，唾液汁或尿液）的钙的方法。 实施方式也可用于临床诊断。

公开(公告)号：US11845733B2

公开(公告)日：2023-12-19

申请号：US17155020

申请日：2021-01-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Dieter Heindl ,  Hans-Peter Josel ,  Uwe Kobold ,  Christoph Seidel ,  Martin Rempt ,  Andreas Leinenbach ,  Giuseppe Prencipe ,  Silvia Baecher ,  Simon Ferdinand Loibl ,  Anna-Skrollan Geiermann ,  Jelena Milic ,  Nicole Pirkl

IPC: C07D249/04 ,  C07D257/04 ,  C07D401/04 ,  C07D401/14 ,  C07D403/04 ,  C07D403/06 ,  C07D471/04 ,  C07D249/06 ,  C07D203/04 ,  G01N30/72

CPC classification number: C07D249/06 ,  C07D203/04 ,  C07D249/04 ,  C07D257/04 ,  C07D401/04 ,  C07D401/14 ,  C07D403/04 ,  C07D403/06 ,  C07D471/04 ,  G01N30/72

Abstract: The present invention relates to reagents which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said reagents.