公开(公告)号：US20170009285A1

公开(公告)日：2017-01-12

申请号：US15121220

申请日：2015-02-03

Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC. ,  DYNAMIC BIOSENSORS GMBH

Inventor： Dieter Heindl ,  Andreas Langer ,  Ulrich Rant ,  Ralf Strasser ,  Michael Schraeml

IPC: C12Q1/68 ,  G01N27/327

Abstract: The present invention relates to a method of identifying a nucleotide at a defined position and determining the sequence of a target polynucleotide using an electro-switchable biosensor, as well as devices comprising an electro-switchable biosensor and uses thereof.

Abstract translation: 本发明涉及一种在确定的位置鉴定核苷酸并使用可电切换生物传感器确定靶多核苷酸的序列的方法，以及包括可电切换生物传感器及其用途的装置。

公开(公告)号：US20160237144A1

公开(公告)日：2016-08-18

申请号：US15025230

申请日：2014-09-22

Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC.

Inventor： Michael Schraeml ,  David Casagolda Vallribera ,  Frank Kroner

IPC: C07K16/12 ,  G01N33/577

CPC classification number: C07K16/1203 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/92 ,  G01N33/577

Abstract: The present description relates to anti-Thermus thermophilus SlyD FKBP domain antibodies and methods of using the same.

Abstract translation: 本说明书涉及抗栖热菌嗜热链球菌SlyD FKBP结构域抗体及其使用方法。

公开(公告)号：US20250043024A1

公开(公告)日：2025-02-06

申请号：US18772484

申请日：2024-07-15

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hartmut Duefel ,  Alfred Engel ,  Frank Kroner ,  Thomas Meier ,  Sandra Rutz ,  Michael Schraeml ,  Gloria Tabares ,  Ulrike Kurtkaya ,  Boris Pinchuk ,  Christina Zimmermann

IPC: C07K16/40 ,  G01N33/543

Abstract: The present invention relates to a novel monoclonal antibody that specifically binds to a conformation dependent epitope on human thymidine kinase 1 (hTK-1; SEQ ID NO:1), to methods for quantifying hTK-1 employing the antibody and to the use of the anti-hTK-1 antibody in quantifying hTK-1

公开(公告)号：US12158466B2

公开(公告)日：2024-12-03

申请号：US16734884

申请日：2020-01-06

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Schraeml ,  Markus Roessler ,  Michael Gerg

IPC: G01N33/543 ,  G01N33/53

Abstract: The present invention relates to a method for determining the total amount and/or concentration of an analyte in the presence of a binding molecule as well as kits, compositions and uses relating thereto.

公开(公告)号：US12077600B2

公开(公告)日：2024-09-03

申请号：US17069349

申请日：2020-10-13

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hartmut Duefel ,  Alfred Engel ,  Frank Kroner ,  Thomas Meier ,  Sandra Rutz ,  Michael Schraeml ,  Gloria Tabares ,  Ulrike Kurtkaya ,  Boris Pinchuk ,  Christina Zimmermann

IPC: G01N31/00 ,  C07K16/40 ,  G01N33/53 ,  G01N33/543

CPC classification number: C07K16/40 ,  G01N33/54326 ,  C07K2317/92

Abstract: The present invention relates to a novel monoclonal antibody that specifically binds to a conformation dependent epitope on human thymidine kinase 1 (hTK-1; SEQ ID NO:1), to methods for quantifying hTK-1 employing the antibody and to the use of the anti-hTK-1 antibody in quantifying hTK-1.

公开(公告)号：US20230406909A1

公开(公告)日：2023-12-21

申请号：US18251393

申请日：2021-10-29

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Ute Jucknischke ,  Ulrike Kurtkaya ,  Thomas Mock ,  Michael Schraeml ,  Sandrine Carolina Stiegler

IPC: C07K16/10

CPC classification number: C07K16/1003 ,  C07K2317/92 ,  C07K2317/94 ,  C07K2317/565 ,  C07K2317/567

Abstract: The present invention relates to monoclonal antibodies binding to the nucleocapsid protein of SARS-CoV-2 virus, nucleic acids encoding said antibody, host cells producing the same, compositions and kits comprising said antibodies, as well as methods of detecting SARS-CoV-2 virus in a sample comprising using said antibodies.

公开(公告)号：US20230382957A1

公开(公告)日：2023-11-30

申请号：US18028245

申请日：2021-09-28

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Mara Boenitz-Dulat ,  Michael Schraeml ,  Bigna Woersdoerfer

IPC: C07K14/245 ,  C12N15/10

CPC classification number: C07K14/245 ,  C12N15/1037 ,  C07K2319/70

Abstract: The present invention relates to bacterial pilus protein complex FimGt-DsF stabilized protein complexes for producing phagemids or filamentous phages, and methods for use of these.

公开(公告)号：US20190381181A1

公开(公告)日：2019-12-19

申请号：US16008281

申请日：2018-06-14

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Schraeml ,  Wojtek Steffen

IPC: A61K47/65 ,  C07K1/13 ,  C12N9/90

Abstract: The present disclosure relates to a recombinant transglutaminase (TG) substrate having an amino acid sequence of the FKBP domain of an FKBP polypeptide, wherein the “insert-in-flap” (IF) domain thereof is, at least in part, replaced by an amino acid sequence (“Q-tag”) of 5 to 20 amino acids with a sequence having at least 80% sequence identity to the YRYRQ portion of the peptide sequence X1-YRYRQ-X2 (SEQ ID NO. 1), and wherein said TG substrate is a substrate for the TG function of the Kutzneria albida TG. The present disclosure furthermore relates to uses of said substrate.

公开(公告)号：US20140120561A1

公开(公告)日：2014-05-01

申请号：US14069445

申请日：2013-11-01

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Herbert Andres ,  Hartmut Duefel ,  Michael Gerg ,  Frank Kowalewsky ,  Christian Scholz ,  Michael Schraeml

IPC: G01N33/74 ,  C07K16/22

CPC classification number: C07K16/22 ,  C07K14/475 ,  C07K14/65 ,  C07K16/1203 ,  C07K16/40 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/23 ,  C07K2319/24 ,  C07K2319/35 ,  C07K2319/41 ,  C07K2319/43 ,  C12N9/90 ,  C12Y502/01008 ,  G01N33/573 ,  G01N33/74 ,  G01N2333/99

Abstract: Isolated antibodies that specifically bind to an epitope comprised in the stretch of amino acids ranging from amino acid 76 to amino acid 84 of human insulin-like growth factor-1 precursor (SEQ ID NO:1). Use of the novel antibodies for the sensitive and specific detection of insulin-like growth factor-1, in some embodiments while in the presence of high excess concentration of insulin-like growth factor-2, for example in a bodily fluid sample.

Abstract translation: 分离的抗体特异性结合包含在人类胰岛素样生长因子-1前体的氨基酸76至氨基酸84的氨基酸区段中的表位（SEQ ID NO：1）。 在一些实施方案中，当存在高过量浓度的胰岛素样生长因子-2（例如在体液样品中）时，使用新型抗体用于敏感和特异性检测胰岛素样生长因子-1。

公开(公告)号：US20130289251A1

公开(公告)日：2013-10-31

申请号：US13923631

申请日：2013-06-21

Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC.

Inventor： Andreas Gallusser ,  Dieter Heindl ,  Michael Schraeml ,  Christoph Seidel ,  Herbert von der Eltz

IPC: C07K19/00

CPC classification number: C07K19/00 ,  C07K16/18 ,  C07K16/2863 ,  C07K16/32 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/626 ,  C07K2317/92 ,  C07K2317/94

Abstract: A binding agent of the Formula A-a′:a-S-b:b′-B:X(n), wherein A as well as B is a monovalent binder, a′:a as well as b:b′ is a binding pair wherein a′ and a do not interfere with the binding of b to b′ and vice versa, S is a spacer of at least 1 nm in length, :X denotes a functional moiety bound either covalently or via a binding pair to at least one of a′, a, b, b′ or S, (n) is an integer and at least 1, - represents a covalent bond, and the linker a-S-b has a length of 6 to 100 nm. Also disclosed are methods of producing such binding agent and certain uses thereof.

Abstract translation: 式A-a'：aSb：b'-B：X（n）的结合剂，其中A以及B是单价键合物，a：a以及b：b'是结合对，其中 a'和a不干扰b与b'的结合，反之亦然，S是长度至少为1nm的间隔物：X表示与共价键或通过结合对键合到至少一个 a'，a，b，b'或S，（n）为整数且至少为1， - 表示共价键，连接体aSb的长度为6〜100nm。 还公开了生产这种结合剂及其某些用途的方法。