公开(公告)号：US20190049460A1

公开(公告)日：2019-02-14

申请号：US16158931

申请日：2018-10-12

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Uwe Kobold ,  Roland Thiele ,  Noah Weiss ,  Benjamin Tiemann

IPC: G01N33/68 ,  G01N33/58 ,  G01N30/72 ,  B01D59/44

Abstract: A method and an apparatus for determining a concentration of a target analyte in a sample of bodily fluid are disclosed. The method involves providing a sample of bodily fluid including the target analyte, providing an internal standard solution including a mixture of components having a plurality of isotopes of the target analyte, wherein a concentration of each isotope is unknown, adding the internal standard solution to the sample, analyzing the sample including the internal standard solution using a mass spectrometer, creating a sample function curve based on signal intensities, wherein the signal intensities define arbitrary units, transferring an analyte signal into a corresponding arbitrary analyte unit by means of the sample function curve, and transferring the arbitrary analyte unit into the concentration of a target analyte by means of a standardization function representing a curve of concentrations depending on the arbitrary units.

公开(公告)号：US12031962B2

公开(公告)日：2024-07-09

申请号：US16438797

申请日：2019-06-12

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Dieter Heindl ,  Thomas Heinemeyer ,  Uwe Kobold ,  Martin Rempt ,  Christoph Seidel

IPC: G01N30/46 ,  G01N30/02 ,  G01N30/06 ,  G01N30/28 ,  G01N30/72 ,  G01N33/58

CPC classification number: G01N30/466 ,  G01N30/06 ,  G01N30/28 ,  G01N30/7233 ,  G01N33/58 ,  G01N2030/027 ,  G01N2030/067

Abstract: A method for identifying a reagent during a process in an analysis system is disclosed. The analysis system comprises a liquid chromatograph and a mass spectrometer. The method comprises providing a reagent, adding at least one chemical substance to the reagent with a concentration being above a detection level of the mass spectrometer, processing the reagent together with the chemical substance with the analysis system, and identifying the reagent based on a detection of a substance detection signal of the mass spectrometer representing the chemical substance.

公开(公告)号：US11730821B2

公开(公告)日：2023-08-22

申请号：US17933906

申请日：2022-09-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hartmut Duefel ,  Uwe Kobold ,  Andreas Leinenbach

IPC: A61K47/68 ,  G01N33/68 ,  G01N30/88

CPC classification number: A61K47/68 ,  G01N33/6848 ,  G01N2030/8868 ,  G01N2458/00

Abstract: The present disclosure is directed at an antibody conjugate having an antibody and a tag, wherein one or more element(s) present in the antibody exhibit an isotope ratio which differs from the naturally occurring isotope ratio of the one or more element(s), wherein the amount of the isotope which is less-common in nature, is increased to at least 4% of the atoms of the respective element in the antibody, as well as uses thereof.

公开(公告)号：US20230160904A1

公开(公告)日：2023-05-25

申请号：US17991366

申请日：2022-11-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Robert Hahn ,  Dieter Heindl ,  Hans-Peter Josel ,  Uwe Kobold ,  Hannes Kuchelmeister ,  Simon Loibl ,  Heribert Maerz ,  Giuseppe Prencipe ,  Martin Rempt

IPC: G01N33/68 ,  C07C243/34 ,  C07D401/04 ,  C07D213/55 ,  C07D213/74 ,  C07D213/56

CPC classification number: G01N33/6848 ,  C07C243/34 ,  C07D401/04 ,  C07D213/55 ,  C07D213/74 ,  C07D213/56 ,  G01N2560/00

Abstract: The present invention relates to compounds which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said compounds.

公开(公告)号：US20230022977A1

公开(公告)日：2023-01-26

申请号：US17933906

申请日：2022-09-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hartmut Duefel ,  Uwe Kobold ,  Andreas Leinenbach

IPC: A61K47/68 ,  G01N33/68

Abstract: The present disclosure is directed at an antibody conjugate having an antibody and a tag, wherein one or more element(s) present in the antibody exhibit an isotope ratio which differs from the naturally occurring isotope ratio of the one or more element(s), wherein the amount of the isotope which is less-common in nature, is increased to at least 4% of the atoms of the respective element in the antibody, as well as uses thereof.

公开(公告)号：US11275065B2

公开(公告)日：2022-03-15

申请号：US16718296

申请日：2019-12-18

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Uwe Kobold ,  Hubert Paul ,  Martin Rempt

IPC: G01N30/72 ,  G01N30/86 ,  G01N30/88 ,  G01N33/487 ,  G01N30/02

Abstract: A diagnostic system and method and an interconnected laboratory system comprising clinical diagnostic systems are presented. The diagnostic system comprises a sample preparation module, a liquid chromatography (LC) separation module coupled to the sample preparation module via a sample preparation/LC interface, a mass spectrometer (MS) module coupled to the LC separation module via an LC/MS interface, and a result calculation module for identifying and/or quantifying analytes or substances of interest contained in the samples and passed through the LC separation and MS modules. The diagnostic system comprises a controller programmed to monitor operational parameters (1-n) indicative of a performance status of the diagnostic system, to trigger a quality control procedure and/or a maintenance procedure whenever one or more parameters (1-n) of the operational parameters (1-n) is out of specification, and to minimize the quality control and/or maintenance procedures as long as the operational parameters (1-n) remains within specification.

公开(公告)号：US20210198217A1

公开(公告)日：2021-07-01

申请号：US17155020

申请日：2021-01-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Dieter Heindl ,  Hans-Peter Josel ,  Uwe Kobold ,  Christoph Seidel ,  Martin Rempt ,  Andreas Leinenbach ,  Giuseppe Prencipe ,  Silvia Baecher ,  Simon Ferdinand Loibl ,  Anna-Skrollan Geiermann ,  Jelena Milic ,  Nicole Pirkl

IPC: C07D249/06 ,  C07D257/04 ,  C07D203/04 ,  G01N30/72

Abstract: The present invention relates to reagents which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said reagents.

公开(公告)号：US10775355B2

公开(公告)日：2020-09-15

申请号：US16009434

申请日：2018-06-15

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Tobias Franz ,  Uwe Kobold ,  Peter Kupser ,  Roland Thiele

IPC: G01N30/88 ,  G01N30/06 ,  G01N30/08 ,  G01N30/24 ,  G01N30/46 ,  G01N30/72 ,  G01N35/00 ,  B01D15/18 ,  G01N30/60 ,  G01N1/40 ,  G01N30/02

Abstract: A clinical diagnostic system is presented and comprises a sample preparation station for automatically preparing samples comprising analytes of interest, a liquid chromatography (LC) separation station comprising a plurality of LC channels and a sample preparation/LC interface for inputting prepared samples into the LC channels. The system further comprises a controller to assign samples to pre-defined sample preparation workflows each comprising a pre-defined sequence of sample preparation steps and requiring a pre-defined time for completion depending on the analytes. The controller further assigns an LC channel for each prepared sample depending on the analytes and plans an LC channel input sequence for inputting the prepared samples that allows analytes from different LC channels to elute in a non-overlapping LC eluate output sequence based on expected elution times. The controller further sets and initiates a start sequence that generates a prepared sample output sequence that matches the LC channel input sequence.

公开(公告)号：US20200246476A1

公开(公告)日：2020-08-06

申请号：US16852931

申请日：2020-04-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Harmut Duefel ,  Uwe Kobold ,  Andreas Leinenbach

IPC: A61K47/68 ,  G01N33/68

Abstract: The present disclosure is directed at an antibody conjugate having an antibody and a tag, wherein one or more element(s) present in the antibody exhibit an isotope ratio which differs from the naturally occurring isotope ratio of the one or more element(s), wherein the amount of the isotope which is less-common in nature, is increased to at least 4% of the atoms of the respective element in the antibody, as well as uses thereof.

公开(公告)号：US20200041470A1

公开(公告)日：2020-02-06

申请号：US16520696

申请日：2019-07-24

Applicant: Roche Diagnostics Operations, Inc. ,  Ludwig-Maximilians-Universitaet Muenchen

Inventor： Thomas Carell ,  Uwe Kobold ,  Dieter Heindl ,  Silvia Baecher ,  Andreas Leinenbach ,  Martin Rempt ,  Toni Pfaffeneder ,  Angie Kirchner ,  Olesea Kosmatchev ,  Rene Rahimoff ,  Sarah Schiffers ,  Markus Muller

IPC: G01N30/72 ,  H01J49/10 ,  H01J49/00 ,  H01J49/06 ,  C07D249/04

Abstract: The present invention relates to reagents suitable in the mass spectrometric determination of analyte molecules such as carbohydrates as well as adducts of such reagents and analyte molecules and applications of said reagents and adducts. Further, the present invention relates to methods for the mass spectrometric determination of analyte molecules.