公开(公告)号：US20050106754A1

公开(公告)日：2005-05-19

申请号：US10932886

申请日：2004-09-01

申请人： Michael Caren ,  Carol Schembri ,  Peter Webb

发明人： Michael Caren ,  Carol Schembri ,  Peter Webb

IPC分类号： B01J19/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

CPC分类号： B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  Y10T436/2575

摘要： A method and apparatus for fabricating an array of biopolymers on a substrate using a biopolymer or biomonomer fluid, and using a dispensing head. The head has a reservoir chamber and at least one jet which can dispense droplets onto a substrate. The jet includes a capillary delivery chamber communicating with the reservoir chamber, which delivery chamber has an orifice. The jet further includes an ejector which, when activated, causes a droplet to be ejected from the orifice. The method includes loading the head by positioning the head with the orifice adjacent and facing a biomonomer or biopolymer fluid, and providing a load pressure to the reservoir chamber. The load pressure is sufficiently negative such that the fluid is drawn into the reservoir chamber through the orifice and delivery chamber, while simultaneously being insufficient to result in ambient atmosphere entering the delivery chamber through the orifice once the head has been loaded and no further fluid is facing and adjacent the orifice. The head is positioned with the orifice facing the substrate and multiple droplets are dispensed from the head orifice so as to form an array of droplets on the substrate.

摘要翻译： 一种使用生物聚合物或生物共聚物流体以及使用分配头在衬底上制造生物聚合物阵列的方法和装置。 头部具有储存室和至少一个可将液滴分配到基底上的射流。 喷射器包括与储存室连通的毛细管输送室，该输送室具有孔口。 射流还包括喷射器，其在被激活时使得液滴从孔口喷出。 该方法包括通过将头部与孔相邻并且面向生物体或生物聚合物流体的位置来装载头部，并向贮存室提供负载压力。 负载压力足够负，使得流体通过孔口和输送室被吸入储存室，同时不足以在头部已经被加载并且没有另外的流体是通过孔口导致环境大气通过孔口进入输送室 面对并邻近孔口。 头部定位成孔口面向衬底，并且从头部孔口分配多个液滴，以便在衬底上形成液滴阵列。

公开(公告)号：US20070201021A1

公开(公告)日：2007-08-30

申请号：US11225743

申请日：2005-09-13

申请人： Carol Schembri ,  Zhenghua Ji ,  Hongfeng Yin ,  William McAllister

发明人： Carol Schembri ,  Zhenghua Ji ,  Hongfeng Yin ,  William McAllister

IPC分类号： G01N21/03

CPC分类号： G01N21/03 ,  G01N21/0303 ,  G01N21/11 ,  G01N21/31 ,  G01N2021/0382 ,  G01N2021/0389

摘要： An apparatus for holding liquid samples.

摘要翻译： 用于保存液体样品的装置。

公开(公告)号：US20070134799A1

公开(公告)日：2007-06-14

申请号：US11701049

申请日：2007-01-31

申请人： Carol Schembri

发明人： Carol Schembri

IPC分类号： G01N33/00

CPC分类号： G01N35/1097 ,  B01L3/502 ,  B01L2300/0636 ,  B01L2300/0877 ,  B01L2400/0409 ,  B01L2400/0633 ,  G01N33/491 ,  Y10T137/108 ,  Y10T436/111666 ,  Y10T436/2575

摘要： The invention relates to a method and an apparatus for performing an assay on chemical, biochemical, or biological fluids. The apparatus includes an assay chamber for fluid reactions and a centrifugal force activated-valve for controlling fluid movement through the assay chamber. An active surface can be positioned in the assay chamber to react with fluids passed through the assay chamber. The active surface may contain biomolecular probes. The biomolecular probes can be DNA, DNA fragments, RNA, RNA fragments, reagents, protein, protein fragments, lipids, and lipid fragments. The apparatus is particularly useful when multiple reactions, dilutions, or washing steps are required to determine a final answer. The centrifugal force activated-valve provides positive control over fluid in the assay chamber and allows for repeated use of the same chamber for multiple reaction steps. The apparatus can be disposed of after an assay to eliminate potential contamination from reuse of the same apparatus. Fluids passed through the assay chamber may be recovered for subsequent analysis, processing, or reactions.

摘要翻译： 本发明涉及用于对化学，生物化学或生物流体进行测定的方法和装置。 该装置包括用于流体反应的测定室和用于控制通过测定室的流体运动的离心力活化阀。 可以将活性表面置于测定室中以与通过测定室的流体反应。 活性表面可以含有生物分子探针。 生物分子探针可以是DNA，DNA片段，RNA，RNA片段，试剂，蛋白质，蛋白质片段，脂质和脂质片段。 当需要多次反应，稀释或洗涤步骤来确定最终答案时，该装置特别有用。 离心力活化阀对分析室中的流体提供积极控制，并允许反复使用相同的室进行多个反应步骤。 该装置可以在分析之后进行处置，以消除重复使用相同装置的潜在污染。 通过测定室的流体可以被回收用于随后的分析，处理或反应。

公开(公告)号：US20060078463A1

公开(公告)日：2006-04-13

申请号：US11236434

申请日：2005-09-26

申请人： Laurence Shea ,  Carol Schembri ,  Douglas Summers ,  Richard Hilson

发明人： Laurence Shea ,  Carol Schembri ,  Douglas Summers ,  Richard Hilson

IPC分类号： G01N1/00

CPC分类号： B01L9/52 ,  B01J2219/00533 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00702 ,  B01L3/502 ,  B01L2300/042 ,  B01L2300/045 ,  B01L2300/0609 ,  B01L2300/0636 ,  B01L2300/0877 ,  Y10T436/25

摘要： Array assay devices and methods for using the same in array based assays are provided. The subject devices are characterized by having a bottom surface, a substrate receiving element and a compression element for holding the bottom surface in a fixed position relative to a substrate received by the receiving element. The subject invention also includes methods for performing an array assay. In the subject methods, a subject device is provided and a substrate having at least one array is placed in the receiving element. The bottom surface of the device is compressed by the compression element so as to fix its position relative to the substrate and a sample is contacted to the at least one array. The subject invention also includes kits which include the subject devices.

公开(公告)号：US20050244972A1

公开(公告)日：2005-11-03

申请号：US11105748

申请日：2005-04-13

申请人： Richard Hilson ,  Edward Donlon ,  Donald Schremp ,  Laurence Shea ,  Carol Schembri ,  Joseph Fredrick

发明人： Richard Hilson ,  Edward Donlon ,  Donald Schremp ,  Laurence Shea ,  Carol Schembri ,  Joseph Fredrick

IPC分类号： G01N35/00 ,  G01N35/04 ,  G01N35/02

CPC分类号： G01N35/04 ,  G01N35/00029 ,  G01N2035/00158 ,  G01N2035/0444 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114998 ,  Y10T436/142222 ,  Y10T436/143333

摘要： Apparatus and methods are disclosed for processing samples on the surface of supports that are contained in support housings. Biopolymer features are attached to the surfaces of the supports. An apparatus comprises an input element, a holding device for holding a plurality of support housings, one or more fluid dispensing stations, and an output element. Each of the support housings contains a support having attached thereto a plurality of biopolymer features. The holding device is movably mounted with respect to other components of the apparatus. The holding device is adapted to receive a support housing from the input element. The output element is adapted to receive a support housing from the holding device. The apparatus is adapted to index each support housing for a predetermined operation. In use, each of the support housings is moved to one or more processing stations by means of the movable holding device. The location and identity of each of the support housings is indexed. Fluid is applied to the surface of each of the supports at the processing stations to process the samples. Each of the support housings is moved away from the fluid dispensing stations, and fluid is physically removed from each of the supports within the support housings.

公开(公告)号：US20050147975A1

公开(公告)日：2005-07-07

申请号：US10746871

申请日：2003-12-24

申请人： Carol Schembri ,  Leslie Leonard

发明人： Carol Schembri ,  Leslie Leonard

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6865

摘要： Methods for amplifying RNA from genomic DNA are provided. In the subject methods, a promoter-primer having a primer domain linked to an RNA polymerase promoter domain is first annealed to genomic DNA. The primer domain of the resultant annealed promoter-primer/genomic DNA complex is then extended to produce a double-stranded DNA molecule that has an RNA polymerase promoter domain. The resultant double-stranded DNA molecule is then transcribed into RNA product, e.g., labeled RNA product, using an RNA polymerase that is able to transcribe through the gap between the 5′ terminus of the promoter domain and the 3′ side of the genomic template. The subject methods find use a variety of different applications in which the preparation of amplified amounts of RNA from a genomic template is desired, where the amplification may be linear or geometric and may amplify the entire genome or only a select portion thereof. Also provided are kits for practicing the subject methods.

摘要翻译： 提供了从基因组DNA扩增RNA的方法。 在本方法中，首先将与RNA聚合酶启动子结构域连接的引物结构域的启动子 - 引物退火至基因组DNA。 然后将所得退火的启动子 - 引物/基因组DNA复合物的引物结构域延伸以产生具有RNA聚合酶启动子结构域的双链DNA分子。 然后将得到的双链DNA分子转录成RNA产物，例如标记的RNA产物，使用能够通过启动子结构域的5'末端和基因组模板的3'侧之间的间隙转录的RNA聚合酶 。 本发明方法发现使用各种不同的应用，其中需要从基因组模板制备扩增量的RNA，其中扩增可以是线性的或几何的，并且可以扩增整个基因组或仅扩增其选择部分。 还提供了用于实践主题方法的工具包。

公开(公告)号：US20050037424A1

公开(公告)日：2005-02-17

申请号：US10640801

申请日：2003-08-13

申请人： Carol Schembri

发明人： Carol Schembri

IPC分类号： B01J19/00 ,  C40B40/06 ,  C40B40/10 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/54313 ,  B01J19/0046 ,  B01J2219/00513 ,  B01J2219/00518 ,  B01J2219/0052 ,  B01J2219/00533 ,  B01J2219/0054 ,  B01J2219/00576 ,  B01J2219/00657 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10

摘要： Devices are disclosed comprising a substrate in the form of an elongated web having on a surface thereof a linear array of chemical compounds and markings on the elongated web indicating segments of the linear array comprising groups of one or more chemical compounds of the linear array. In certain embodiments the devices comprise a severable housing, a linear array of features within the housing, and markings on the housing indicating segments of the linear array comprising groups of one or more features of the linear array. Usually, the housing is an enclosed microchannel. The device may be employed for conducting an assay for one or more analytes suspected of being in a sample.

摘要翻译： 公开的装置包括呈细长网状形式的基底，在其表面上具有在细长网上的化学化合物和标记的线性阵列，指示线性阵列的片段包含线性阵列的一种或多种化学化合物的组。 在某些实施例中，装置包括可分离的壳体，壳体内的特征的线性阵列，以及壳体上的标记，指示线性阵列的线段阵列包括线性阵列的一个或多个特征的组。 通常，外壳是封闭的微通道。 该装置可用于对疑似在样品中的一种或多种分析物进行测定。

公开(公告)号：US5599411A

公开(公告)日：1997-02-04

申请号：US350856

申请日：1994-12-06

申请人： Carol Schembri

发明人： Carol Schembri

IPC分类号： B29C65/08 ,  B32B31/16

CPC分类号： B29C65/08 ,  B29C66/73921 ,  B29C66/80 ,  B29C66/71 ,  B29C66/9513

摘要： A method and a device for modifying the energy-applying surface of an ultrasonic welding horn to join two complex thermoplastic parts by applying a thin film to the surface of the horn is disclosed. The thin film is applied to insure that a proper weld occurs along a complex and intricate pattern of energy directors located between the parts.

摘要翻译： 公开了一种用于通过将薄膜施加到喇叭表面来改变超声焊接喇叭的能量施加表面以连接两个复合热塑性部件的方法和装置。 应用薄膜来确保位于两个部件之间的复杂且复杂的能量导向器的适当焊接发生。