公开(公告)号：US07393668B2

公开(公告)日：2008-07-01

申请号：US10541389

申请日：2004-01-20

申请人： Yoshifumi Yanagita ,  Noriko Ogawa ,  Yasuyoshi Ueda ,  Fumio Osakada ,  Keiji Matsumoto

发明人： Yoshifumi Yanagita ,  Noriko Ogawa ,  Yasuyoshi Ueda ,  Fumio Osakada ,  Keiji Matsumoto

IPC分类号： C12P7/62 ,  C12N9/00

CPC分类号： C12P7/625

摘要： The present invention has an object to provide a method for separating and purifying a PHA without causing a serious decrease of the molecular weight to obtain a highly pure PHA in a high yield, which comprises efficiently removing cell components other than PHA particles from a cultured PHA-containing microbial cell. Another object of the present invention is to provide a method for obtaining an agglomerate of PHA particles.The method for recovering a PHA according to the present invention is a method which comprises efficiently disrupting a cell to recover the PHA by carrying out a physical disruption treatment and an alkali addition at low temperature for an aqueous suspension of the PHA-containing microbial cell, and then treating the PHA with an enzyme and/or a surfactant. Moreover, the particle diameter of the PHA may be enlarged by suspending the PHA in a hydrophilic solvent and/or water, and stirring at a temperature equal to or below the boiling point of said suspension, to agglomerate said PHA.

摘要翻译： 本发明的目的是提供一种分离纯化PHA的方法，而不会导致分子量的严重降低以获得高产率的高纯度PHA，其包括从培养的PHA有效除去PHA颗粒以外的细胞成分 含微生物细胞。 本发明的另一个目的是提供一种获得PHA颗粒附聚物的方法。 根据本发明的回收PHA的方法是通过对含PHA的微生物细胞的水性悬浮液进行物理破坏处理和低温碱添加来有效地破坏细胞以回收PHA的方法， 然后用酶和/或表面活性剂处理PHA。 此外，通过将PHA悬浮在亲水溶剂和/或水中并在等于或低于所述悬浮液沸点的温度下搅拌以使所述PHA聚集，可以扩大PHA的粒径。

公开(公告)号：US20070161825A1

公开(公告)日：2007-07-12

申请号：US11716828

申请日：2007-03-12

申请人： Takahiro Ueda ,  Shiro Kitamura ,  Yasuyoshi Ueda

发明人： Takahiro Ueda ,  Shiro Kitamura ,  Yasuyoshi Ueda

IPC分类号： C07C43/20

CPC分类号： C07C41/26 ,  C07C41/34 ,  C07C43/23

摘要： The present invention provides a method for obtaining reduced coenzyme Q10 which is useful as an ingredient in foods, functional nutritive foods, specific health foods, nutritional supplements, nutrients, drinks, feeds, cosmetics, medicines, remedies, preventive drugs, etc. suited for a commercial scale production in high quality and efficiently. The high-quality oily reduced coenzyme Q10 which has low viscosity and thereby easily handled may be produced by separating an aqueous phase from the reaction mixture obtainable by oily reacting oxidized coenzyme Q10 with a reducing agent in water, or by distilling off an coexisting organic solvent at or above the melting temperature of reduced coenzyme Q10 in concentrating the organic phase containing reduced coenzyme Q10. Moreover, a solution or slurry of reduced coenzyme Q10 may be obtained by adding a desired solvent to the obtained oily product, or a solid of reduced coenzyme Q10 may be produced by contacting the oily product with a seed crystal.

公开(公告)号：US20060084161A1

公开(公告)日：2006-04-20

申请号：US10541389

申请日：2004-01-20

申请人： Yoshifumi Yanagita ,  Noriko Ogawa ,  Yasuyoshi Ueda ,  Fumio Osakada ,  Keiji Matsumoto

发明人： Yoshifumi Yanagita ,  Noriko Ogawa ,  Yasuyoshi Ueda ,  Fumio Osakada ,  Keiji Matsumoto

IPC分类号： C12N9/00

CPC分类号： C12P7/625

摘要： The present invention has an object to provide a method for separating and purifying a PHA without causing a serious decrease of the molecular weight to obtain a highly pure PHA in a high yield, which comprises efficiently removing cell components other than PHA particles from a cultured PHA-containing microbial cell. Another object of the present invention is to provide a method for obtaining an agglomerate of PHA particles. The method for recovering a PHA according to the present invention is a method which comprises efficiently disrupting a cell to recover the PHA by carrying out a physical disruption treatment and an alkali addition at low temperature for an aqueous suspension of the PHA-containing microbial cell, and then treating the PHA with an enzyme and/or a surfactant. Moreover, the particle diameter of the PHA may be enlarged by suspending the PHA in a hydrophilic solvent and/or water, and stirring at a temperature equal to or below the boiling point of said suspension, to agglomerate said PHA.

摘要翻译： 本发明的目的是提供一种分离纯化PHA的方法，而不会导致分子量的严重降低以获得高产率的高纯度PHA，其包括从培养的PHA有效除去PHA颗粒以外的细胞成分 含微生物细胞。 本发明的另一个目的是提供一种获得PHA颗粒附聚物的方法。 根据本发明的回收PHA的方法是通过对含PHA的微生物细胞的水性悬浮液进行物理破坏处理和低温碱添加来有效地破坏细胞以回收PHA的方法， 然后用酶和/或表面活性剂处理PHA。 此外，通过将PHA悬浮在亲水溶剂和/或水中并在等于或低于所述悬浮液沸点的温度下搅拌以使所述PHA聚集，可以扩大PHA的粒径。

公开(公告)号：US20050222373A1

公开(公告)日：2005-10-06

申请号：US10992132

申请日：2004-11-19

申请人： Koichi Kinoshita ,  Fumio Osakada ,  Yasuyoshi Ueda ,  Karunakaran Narasimhan ,  Angella Cearley ,  Kenneth Yee ,  Isao Noda

发明人： Koichi Kinoshita ,  Fumio Osakada ,  Yasuyoshi Ueda ,  Karunakaran Narasimhan ,  Angella Cearley ,  Kenneth Yee ,  Isao Noda

IPC分类号： C08G67/00

CPC分类号： C08G63/90 ,  C08G63/06

摘要： The present invention provides a method for easily obtaining a biodegradable polyhydroxyalkanoate by a solvent extraction method. A method for producing a polyhydroxyalkanoate crystal which comprises mixing a solution of a polyhydroxyalkanoate in a good solvent with a poor solvent at 50 to 130° C. to precipitate a polyhydroxyalkanoate.

摘要翻译： 本发明提供了一种通过溶剂萃取方法容易地获得可生物降解的聚羟基链烷酸酯的方法。 一种制备聚羟基链烷酸酯晶体的方法，其包括在50-130℃下将聚羟基链烷酸酯在良溶剂中的溶液与不良溶剂混合以沉淀聚羟基链烷酸酯。

公开(公告)号：US20050118288A1

公开(公告)日：2005-06-02

申请号：US10504256

申请日：2003-02-20

申请人： Koki Yamashita ,  Tadao Ono ,  Shiro Kitamura ,  Yasuyoshi Ueda

发明人： Koki Yamashita ,  Tadao Ono ,  Shiro Kitamura ,  Yasuyoshi Ueda

IPC分类号： A23K1/14 ,  A23K1/16 ,  A23L1/236 ,  A23L1/30 ,  A61K9/20 ,  A61K31/352 ,  A61K31/37 ,  A61K31/56 ,  A61K36/484 ,  A61P3/04 ,  A61P3/06 ,  A61P3/10 ,  A61P9/10 ,  A61P9/12 ,  A61P43/00 ,  C07J63/00 ,  A61K35/78

CPC分类号： C07J63/00 ,  A23K10/30 ,  A23K20/10 ,  A23L27/30 ,  A23L33/105 ,  A23V2002/00 ,  A61K31/352 ,  A61K31/37 ,  A61K31/56 ,  A61K36/484 ,  A23V2250/252

摘要： It is an object of the present invention to provide a method for producing a hydrophobic licorice extract which is useful for applications, such as food, food additives, functional nutritive food, food for specified health uses, dietary supplements, drink, feedstuff, drugs, and quasi-drugs, and which has satisfactory powder characteristics. This invention relates to a method for producing a hydrophobic licorice extract comprises performing extraction from licorice with a water-soluble organic solvent, the area of the bark of the licorice occupying at least 30% of the total surface area of the licorice. This invention also relates to a method for producing a hydrophobic licorice extract comprises crystallizing an extract extracted from licorice, or an extraction residue of the licorice, with a water-soluble organic solvent in a mixed solvent comprising water and the water-soluble organic solvent, the weight ratio, water/(water-soluble organic solvent+water), being ⅓ or more.

摘要翻译： 本发明的目的是提供一种制备疏水性甘草提取物的方法，其可用于食品，食品添加剂，功能性营养食品，特定健康用途食品，膳食补充剂，饮料，饲料，药物， 和准药，具有令人满意的粉末特性。 本发明涉及一种生产疏水甘草提取物的方法，包括用水溶性有机溶剂从甘草中提取甘草的皮肤面积占甘草总表面积的至少30％。 本发明还涉及一种疏水性甘草提取物的制造方法，其特征在于，包括使水溶性有机溶剂在水和水溶性有机溶剂的混合溶剂中结晶从甘草提取的提取物或甘草的提取残渣， 重量比，水/（水溶性有机溶剂+水）为1/3以上。

公开(公告)号：US06737264B1

公开(公告)日：2004-05-18

申请号：US09869297

申请日：2001-11-02

申请人： Katsuji Maehara ,  Shigeru Kawano ,  Makoto Yamaguchi ,  Yasuyoshi Ueda

发明人： Katsuji Maehara ,  Shigeru Kawano ,  Makoto Yamaguchi ,  Yasuyoshi Ueda

IPC分类号： C12P4100

CPC分类号： C12P41/002 ,  C07C269/08 ,  C12P13/02 ,  C07C271/16

摘要： The present invention has for its object to provide a practical method for the purification and isolation on a commercial scale of said compound (1) or compound (2) in good yield and with high quality. The present invention provides a purification/isolation method of a (2S,3S)-1-halo-2-hydroxy-3-N-(tert-butoxycarbonyl)amino-4-phenylbutane (1) or a (2R,3S)-1-halo-2-hydroxy-3-N-(tert-butoxycarbonyl)amino-4-phenylbutane (2) which comprises, for the purpose of removing contaminant impurity from a mixture containing at least one of said compounds (1) and (2), causing the objective compound (1) or compound (2) to be crystallized in the presence of a solvent comprised of a hydrocarbon solvent and then collecting the obtained crystals.

摘要翻译： 本发明的目的是提供一种以高产率和高质量在商业规模上纯化和分离所述化合物（1）或化合物（2）的实用方法。本发明提供了一种纯化/分离方法 （2S，3S）-1-卤代-2-羟基-3-N-（叔丁氧基羰基）氨基-4-苯基丁烷（1）或（2R，3S）-1-卤代-2-羟基-3- N-（叔丁氧基羰基）氨基-4-苯基丁烷（2），其包含为了从含有至少一种所述化合物（1）和（2）的混合物除去污染物杂质的目的化合物（1） 或化合物（2）在由烃溶剂组成的溶剂的存在下结晶，然后收集所获得的晶体。

公开(公告)号：US06720449B2

公开(公告)日：2004-04-13

申请号：US09926346

申请日：2002-02-05

申请人： Masanobu Sugawara ,  Akio Fujii ,  Kazumi Okuro ,  Yasuhiro Saka ,  Nobuo Nagashima ,  Kenji Inoue ,  Toshihiro Takeda ,  Koichi Kinoshita ,  Tadashi Moroshima ,  Yoshihide Fuse ,  Yasuyoshi Ueda

发明人： Masanobu Sugawara ,  Akio Fujii ,  Kazumi Okuro ,  Yasuhiro Saka ,  Nobuo Nagashima ,  Kenji Inoue ,  Toshihiro Takeda ,  Koichi Kinoshita ,  Tadashi Moroshima ,  Yoshihide Fuse ,  Yasuyoshi Ueda

IPC分类号： C07D20308

CPC分类号： C07D203/08 ,  C07B2200/07 ,  C07C227/10 ,  C07C227/32 ,  C07C227/42 ,  C07C229/30 ,  C07C303/36 ,  C07D203/20 ,  C07D203/24 ,  C07C311/19 ,  C07C229/26

摘要： An optically active amino acid derivative is produced by N-protecting an optically active 3-haloalanine derivative followed by cyclization, or cyclizing this derivative followed by N-protection to thereby give an optically active N-protected-aziridine-2-carboxylic acid derivative which is protected by a benzenesulfonyl group substituted by nitro at the 2- and/or 4-positions and then treating this product with an organic metal reagent, or by N-protecting an optically active 3-haloalanine derivative to thereby give N-protected-aziridine-2-carboxylic acid which is protected by a benzenesulfonyl group substituted by nitro at the 2- and/or 4-positions and then treating this product with an organic metal reagent. According to this process, natural and unnatural optically active amino acids can be produced from inexpensive materials by using simple procedures.

摘要翻译： 光学活性氨基酸衍生物通过N-保护光学活性3-卤代丙氨酸衍生物进行环化生成，或使该衍生物环化，接着进行N-保护，从而得到光学活性N-保护的氮丙啶-2-羧酸衍生物， 在2-和/或4-位被硝基取代的苯磺酰基保护，然后用有机金属试剂处理该产物，或通过N-保护光学活性3-卤代丙氨酸衍生物，由此得到N-保护的氮丙啶 -2-羧酸，其在2-和/或4-位被硝基取代的苯磺酰基保护，然后用有机金属试剂处理该产物。根据该方法，天然和非天然旋光氨基酸可以是 通过简单的程序从便宜的材料生产。

公开(公告)号：US06271393B1

公开(公告)日：2001-08-07

申请号：US09554827

申请日：2000-07-17

申请人： Tadashi Moroshima ,  Yoshifumi Yanagida ,  Yoshihide Fuse ,  Yasuyoshi Ueda

发明人： Tadashi Moroshima ,  Yoshifumi Yanagida ,  Yoshihide Fuse ,  Yasuyoshi Ueda

IPC分类号： C07D20706

CPC分类号： C07K5/0222

摘要： The process for producing N2-(1(S)-carboxy-3-phenylpropyl)-L-lysyl-L-proline in a simple, efficient and industrially advantageous manner, including the following steps: 1) subjecting N2-(1(S)-alkoxycarbonyl-3-phenylpropyl)-N6-trifluoroacetyl-L-lysyl-L-proline (1) to alkali hydrolysis in a mixed solution composed of water and a hydrophilic organic solvent using an inorganic base in an amount of n molar equivalents (n ≧ 3) per mole of the above compound (1), 2) neutralizing the hydrolysis product using an inorganic acid in an amount of (n − 1) to n molar equivalents (n ≧ 3) to form a compound (2) and removing the inorganic salt formed by causing the same to precipitate out from a solvent system suited for decreasing the solubility of the inorganic salt, and 3) causing the compound (2) existing in the mixture after removal of the inorganic salt to crystallize out at the isoelectric point thereof and thereby recovering the compound (2) in the form of crystals while retaining the salts including the trifluoroacetic acid-derived organic acid salt in a state dissolved in the mother liquor.

摘要翻译： 包括以下步骤制备N2-（1（S） - 羧基-3-苯基丙基）-L-赖氨酰-L-脯氨酸的方法，包括以下步骤：1）使N2-（1（S ） - 烷氧基羰基-3-苯基丙基）-N6-三氟乙酰基-L-赖氨酰-L-脯氨酸（1）在由水和亲水性有机溶剂组成的混合溶液中使用n摩尔当量的无机碱进行碱水解（ n）= 3），2）使用（n-1）〜n摩尔当量（n> = 3）的无机酸中和水解产物，形成化合物（2） ），并且通过使其从适合于降低无机盐的溶解度的溶剂体系中析出而形成的无机盐，以及3）除去无机盐以使其结晶出来，使存在于混合物中的化合物（2）结晶出来 其等电点，从而以晶体的形式回收化合物（2），同时保留盐 将三氟乙酸衍生的有机酸盐以溶解在母液中的状态。

公开(公告)号：US5717077A

公开(公告)日：1998-02-10

申请号：US570415

申请日：1995-12-11

申请人： Toshiyuki Araki ,  Takemi Tokieda ,  Shin-ichi Yabushita ,  Yasuyoshi Ueda ,  Bunzi Tsukise

发明人： Toshiyuki Araki ,  Takemi Tokieda ,  Shin-ichi Yabushita ,  Yasuyoshi Ueda ,  Bunzi Tsukise

IPC分类号： C09B62/44 ,  C09B62/503 ,  C09B47/24 ,  C09B62/085

CPC分类号： C09B62/503 ,  C09B62/4403

摘要： A phthalocyanine monoazo compound which is represented by the following formula (I): ##STR1## wherein Pc represents a phthalocyanine group; M represents nickel, cobalt or copper; R.sub.1 represents a hydrogen atom or a lower alkyl group; R.sub.2 and R.sub.3 represent a hydrogen atom, a lower alkyl group, a lower alkoxy group or a sulfo group; R.sub.4 represents a hydrogen or a lower alkyl group; A represents a group containing a triazine group or a pyrazole group and/or --SO.sub.2 X wherein X represents --CH.dbd.CH.sub.2 or --CH.sub.2 CH.sub.2 Y and Y represents a group splittable by the action of an alkali; p is a number of 0-2; q is a number of 1-3; r is a number of 1-2; p+q+r is 4 or less or which is a salt of the compound of formula (I).

摘要翻译： 由下式（I）表示的酞菁单偶氮化合物：其中Pc表示酞菁基团; M表示镍，钴或铜; R1表示氢原子或低级烷基; R2和R3表示氢原子，低级烷基，低级烷氧基或磺基; R4表示氢或低级烷基; A表示含有三嗪基或吡唑基和/或-SO 2 X的基团，其中X表示-CH = CH 2或-CH 2 CH 2 Y，Y表示可通过碱的作用而分裂的基团; p是0-2的数; q是1-3的数量; r是1-2的数量; p + q + r为4以下，或者为式（I）的化合物的盐。

公开(公告)号：US4994600A

公开(公告)日：1991-02-19

申请号：US869782

申请日：1986-06-02

申请人： Satomi Takahashi ,  Yasuyoshi Ueda ,  Yoshifumi Yanagida ,  Namito Yoshio ,  Takehisa Ohashi ,  Kiyoshi Watanabe

发明人： Satomi Takahashi ,  Yasuyoshi Ueda ,  Yoshifumi Yanagida ,  Namito Yoshio ,  Takehisa Ohashi ,  Kiyoshi Watanabe

IPC分类号： C07C69/738 ,  B01J27/02 ,  B01J31/02 ,  B01J31/14 ,  C07B61/00 ,  C07C67/327

CPC分类号： C07C67/327

摘要： A process for preparing trans-.beta.-benzoylacrylic acid ester having the general formula (I): ##STR1## wherein R is alkyl group or aralkyl group, which comprises dealcoholizing .beta.-benzoyl-.alpha.-alkoxypropionic acid ester having the general formula (II): ##STR2## wherein R is above, in the presence of an acid catalyst to give trans-.beta.-benzoylacrylic acid ester having the general formula (I).According to the process of the present invention, the by-product (II) produced in the esterification reaction of .beta.-benzoylacrylic acid (III) with the alcohol (V) by the dehydration reaction can be converted into the compound (I) by the dealcoholization reaction in the presence of the acid catalyst and thus trans-.beta.-benzoylacrylic acid ester (I) with a high purity can be produced in an industrially advantageous manner.

摘要翻译： 制备具有通式（I）的反式-β-苯甲酰基丙烯酸酯的方法：其中R是烷基或芳烷基，其包括使具有通式（I）的β-苯甲酰基-α-烷氧基丙酸酯脱醇 （II）：其中R在上面，在酸催化剂存在下，得到具有通式（I）的反式-β-苯甲酰基丙烯酸酯。 根据本发明的方法，通过脱水反应在β-苯甲酰基丙烯酸（III）与醇（V）的酯化反应中产生的副产物（II）可以通过 在酸催化剂存在下脱醇反应，从而可以以工业上有利的方式生产具有高纯度的反式-β-苯甲酰基丙烯酸酯（I）。