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公开(公告)号:US10577652B2
公开(公告)日:2020-03-03
申请号:US16200571
申请日:2018-11-26
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C12Q1/68 , C12Q1/6869 , C07H21/00 , C12Q1/686 , C12Q1/6874 , C12Q1/6872 , C07H19/14 , C07H19/10 , C12Q1/6876 , C40B40/00
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US20190092805A1
公开(公告)日:2019-03-28
申请号:US16200557
申请日:2018-11-26
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C07H19/14 , C12Q1/68 , C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q1/6876 , C12Q1/6872 , C07H19/10 , C07H21/00 , C40B40/00
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US09708358B2
公开(公告)日:2017-07-18
申请号:US15380284
申请日:2016-12-15
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
CPC分类号: C07H19/10 , C07B2200/11 , C07H19/14 , C07H21/00 , C12Q1/686 , C12Q1/6869 , C12Q1/6872 , C12Q1/6874 , C40B40/00 , C12Q2565/501 , C12Q2563/107 , C12Q2535/101 , C12Q2525/186 , C12Q2535/122
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US20170190730A2
公开(公告)日:2017-07-06
申请号:US15167917
申请日:2016-05-27
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
CPC分类号: C07H19/14 , C07B2200/11 , C07H19/10 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6869 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C40B40/00 , C12Q2565/501 , C12Q2525/186
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US20170088575A1
公开(公告)日:2017-03-30
申请号:US15380270
申请日:2016-12-15
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
CPC分类号: C07H19/14 , C07B2200/11 , C07H19/10 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6869 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C40B40/00 , C12Q2565/501 , C12Q2525/186
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US09133511B2
公开(公告)日:2015-09-15
申请号:US13959660
申请日:2013-08-05
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
CPC分类号: C07H19/14 , C07B2200/11 , C07H19/10 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6869 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C40B40/00 , C12Q2565/501 , C12Q2525/186
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
摘要翻译: 本发明提供了通过在聚合酶反应中将核苷酸类似物掺入DNA的生长链中之后检测核苷酸类似物的同一性来提供将核酸连接到固体表面和测序核酸的方法。 本发明还提供核苷酸类似物,其包含通过可切割连接子连接至核苷酸类似物的独特标记,以及可脱氧化学基团以封端脱氧核糖3'-位上的-OH基团。
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公开(公告)号:US10669582B2
公开(公告)日:2020-06-02
申请号:US15915983
申请日:2018-03-08
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C12Q1/68 , C07H19/14 , C12Q1/6869 , C07H21/00 , C12Q1/686 , C12Q1/6874 , C12Q1/6872 , C07H19/10 , C12Q1/6876 , C40B40/00
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US10570446B2
公开(公告)日:2020-02-25
申请号:US16200540
申请日:2018-11-26
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C12Q1/68 , C07H19/14 , C12Q1/6869 , C07H21/00 , C12Q1/686 , C12Q1/6874 , C12Q1/6872 , C07H19/10 , C12Q1/6876 , C40B40/00
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US10457984B2
公开(公告)日:2019-10-29
申请号:US16150180
申请日:2018-10-02
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C12Q1/68 , C07H19/14 , C12Q1/6869 , C07H21/00 , C12Q1/686 , C12Q1/6874 , C12Q1/6872 , C07H19/10 , C12Q1/6876 , C40B40/00
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US10407458B2
公开(公告)日:2019-09-10
申请号:US16149098
申请日:2018-10-01
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C07H19/14 , C12Q1/68 , C07H21/00 , C12Q1/686 , C12Q1/6874 , C12Q1/6872 , C12Q1/6869 , C07H19/10 , C12Q1/6876 , C40B40/00
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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