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公开(公告)号：US11359185B2
公开(公告)日：2022-06-14
申请号：US15064792
申请日：2016-03-09
发明人： Christopher Bieniarz , Jerome W Kosmeder , Mark Lefever , Casey A Kernag , Julia Ashworth-Sharpe , Jennifer Wong
IPC分类号： G01N33/543 , G01N33/552 , C12N9/16 , A61K47/68 , A61K47/65 , G01N33/535 , C07K16/42 , C07K16/44 , C12N9/08 , C12N9/96 , G01N33/53
摘要： Antibody/signal-generating moiety conjugates are disclosed that include an antibody covalently linked to a signal-generating moiety through a heterobifunctional polyalkyleneglycol linker. The disclosed conjugates show exceptional signal-generation in immunohistochemical and in situ hybridization assays on tissue sections and cytology samples. In one embodiment, enzyme-metallographic detection of nucleic acid sequences with hapten-labeled probes can be accomplished using the disclosed conjugates as a primary antibody without amplification.
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22.发明授权公开(公告)号：US11143648B2
公开(公告)日：2021-10-12
申请号：US16226427
申请日：2018-12-19
IPC分类号： G01N33/533 , C09B11/24 , C09B23/08 , G01N33/58 , G01N33/532
摘要： Disclosed herein are novel chromogenic conjugates, the conjugates comprising at least two detectable moieties.
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公开(公告)号：US10883999B2
公开(公告)日：2021-01-05
申请号：US16055046
申请日：2018-08-04
发明人： Rui Hong , Hong Wang , Mark Lefever , Jan Froehlich , Christopher Bieniarz , Brian Daniel Kelly , Phillip Miller
摘要： Particular disclosed embodiments disclosed herein concern using a one or more various mass tags, which can be specifically deposited at targets through direct or indirect enzymatic-catalyzed transformation, to provide a method for identifying targets in tissue samples. The mass tags may be labeled with stable isotopes to produce mass tags having the same chemical structure but different masses. Mass codes produced by ionizing the mass tags are detected and/or quantified using mass spectrometry. The method can be used for multiplexed detection of multiple targets in a particular sample. In some embodiments, a map divided into sections representing sections of the tissue sample may be prepared, with the map sections including data corresponding to quantification data wherein the size of a mass peak is determined and correlated with the amount of a target for the corresponding tissue sample section.
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公开(公告)号：US20200326345A1
公开(公告)日：2020-10-15
申请号：US16786647
申请日：2020-02-10
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder, II , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , C12Q1/682 , C12Q1/68 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US20190018018A1
公开(公告)日：2019-01-17
申请号：US16038374
申请日：2018-07-18
发明人： Nelson ALEXANDER , William Day , Jerome W. Kosmeder, II , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , G01N33/542 , C12Q1/68 , G01N33/53 , C12Q1/682
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US20160187324A1
公开(公告)日：2016-06-30
申请号：US15064792
申请日：2016-03-09
发明人： Christopher Bieniarz , Jerome W. Kosmeder , Mark Lefever , Casey A. Kernag , Julia Ashworth-Sharpe , Jennifer Wong
IPC分类号： G01N33/535 , C12N9/16 , C12N9/96 , C07K16/44 , A61K47/48
CPC分类号： C12N9/16 , A61K47/65 , A61K47/6815 , C07K16/42 , C07K16/44 , C07K2317/76 , C12N9/0065 , C12N9/96 , G01N33/535
摘要： Antibody/signal-generating moiety conjugates are disclosed that include an antibody covalently linked to a signal-generating moiety through a heterobifunctional polyalkyleneglycol linker. The disclosed conjugates show exceptional signal-generation in immunohistochemical and in situ hybridization assays on tissue sections and cytology samples. In one embodiment, enzyme-metallographic detection of nucleic acid sequences with hapten-labeled probes can be accomplished using the disclosed conjugates as a primary antibody without amplification.
摘要翻译： 公开了抗体/信号产生部分缀合物，其包括通过异双功能聚亚烷基二醇连接体与产生信号的部分共价连接的抗体。 所公开的缀合物在组织切片和细胞学样品的免疫组织化学和原位杂交测定中显示出异常的信号产生。 在一个实施方案中，用半抗原标记的探针对核酸序列的酶 - 金相检测可以使用所公开的缀合物作为一级抗体而不进行扩增来完成。
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公开(公告)号：US20140147906A1
公开(公告)日：2014-05-29
申请号：US14146389
申请日：2014-01-02
发明人： Christopher Bieniarz , Jennifer Wong , Mark Lefever , Jerome W. Kosmeder, II , Julia Ashworth-Sharpe , Casey A. Kernag
CPC分类号： G01N33/535 , A61K47/65 , A61K47/6815 , C07K16/42 , C07K16/44 , C07K2317/76 , C12N9/0065 , C12N9/16 , C12N9/96
摘要： Antibody/signal-generating moiety conjugates are disclosed that include an antibody covalently linked to a signal-generating moiety through a heterobifunctional polyalkyleneglycol linker. The disclosed conjugates show exceptional signal-generation in immunohistochemical and in situ hybridization assays on tissue sections and cytology samples. In one embodiment, enzyme-metallographic detection of nucleic acid sequences with hapten-labeled probes can be accomplished using the disclosed conjugates as a primary antibody without amplification.
摘要翻译： 公开了抗体/信号产生部分缀合物，其包括通过异双功能聚亚烷基二醇连接体与产生信号的部分共价连接的抗体。 所公开的缀合物在组织切片和细胞学样品的免疫组织化学和原位杂交测定中显示出异常的信号产生。 在一个实施方案中，用半抗原标记的探针对核酸序列的酶 - 金相检测可以使用所公开的缀合物作为一级抗体而无需扩增来完成。
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公开(公告)号：US20240357219A1
公开(公告)日：2024-10-24
申请号：US18760867
申请日：2024-07-01
CPC分类号： H04N23/11 , G01N21/6458
摘要： Disclosed herein are embodiments of imaging biological specimens. An imaging system can include a microscope for directly viewing the biological specimen and a multi-spectral imaging apparatus for outputting digitally enhanced images, near-video rate imaging, and/or videos of the specimen. An imaging system can include a digital scanner that digitally processes images to produce a composite image with enhanced color contrast of features of interest.
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公开(公告)号：US20220178934A1
公开(公告)日：2022-06-09
申请号：US17678389
申请日：2022-02-23
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder, II , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , C12Q1/682 , C12Q1/68 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US20200333349A1
公开(公告)日：2020-10-22
申请号：US16856604
申请日：2020-04-23
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , C12Q1/682 , C12Q1/68 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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