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142 条记录 (耗时 0.049 秒)

    Compositions and methods for detecting small RNAs, and uses thereof
    23.
    发明授权
    Compositions and methods for detecting small RNAs, and uses thereof 有权
    用于检测小RNA的组合物和方法及其用途

    公开(公告)号：US08399222B2

    公开(公告)日：2013-03-19

    申请号：US12625609

    申请日：2009-11-25

    申请人： Amara Siva ,  Mark Alan Reynolds

    发明人： Amara Siva ,  Mark Alan Reynolds

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6876 ,  C12Q1/6853 ,  C12Q2600/178 ,  C12Q2565/537 ,  C12Q2565/525 ,  C12Q2537/143 ,  C12Q2525/301

    摘要： Compositions and methods are provided for the detection of small RNA target nucleic acids, preferably miRNA target nucleic acids, wherein the compositions and methods provide for sensitive and specific detection of the target nucleic acids. The compositions and methods include using one or more of a first amplification oligomer that is preferably an extender primer, a target capture oligomer that is preferably at least partially double stranded, a promoter primer/provider, a reverse primer that is preferably a universal primer and a detection probe. The compositions and methods are useful for diagnostics, prognostics, monitoring the effectiveness of treatment and/or determining a treatment.

    摘要翻译： 提供组合物和方法用于检测小RNA靶核酸，优选miRNA靶核酸，其中组合物和方法提供对靶核酸的敏感和特异性检测。 组合物和方法包括使用一种或多种优选扩增引物的第一扩增寡聚物，优选至少部分双链的靶捕获低聚物，启动子引物/提供者，优选通用引物的反向引物和 检测探针。 组合物和方法可用于诊断，预后，监测治疗的有效性和/或确定治疗。

    Detection of nucleic acids by multiple sequential invasive cleavages
    24.
    发明授权
    Detection of nucleic acids by multiple sequential invasive cleavages 失效
    通过多次连续侵入裂解检测核酸

    公开(公告)号：US08288093B2

    公开(公告)日：2012-10-16

    申请号：US12099656

    申请日：2008-04-08

    申请人： Jeff G. Hall ,  Victor I. Lyamichev ,  Andrea L. Mast ,  Mary Ann D. Brow

    发明人： Jeff G. Hall ,  Victor I. Lyamichev ,  Andrea L. Mast ,  Mary Ann D. Brow

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N9/22 ,  C12Q1/6823 ,  C12Q1/6827 ,  Y10S435/81 ,  Y10S435/822 ,  C12Q2565/525 ,  C12Q2561/109 ,  C12Q2525/301

    摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.

    摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。 本发明还涉及基于电荷分离核酸分子的方法和装置。 本发明还提供了通过形成完整和活化的蛋白质结合区域来检测非靶切割产物的方法。 本发明还提供用于检测样品中巨细胞病毒核酸的敏感和具体方法。

    Methods and compositions to detect nucleic acids in a biological sample
    26.
    发明授权
    Methods and compositions to detect nucleic acids in a biological sample 有权
    检测生物样品中核酸的方法和组合物

    公开(公告)号：US08034554B2

    公开(公告)日：2011-10-11

    申请号：US11173915

    申请日：2005-06-29

    申请人： Michael M. Becker ,  Mehrdad R. Majlessi

    发明人： Michael M. Becker ,  Mehrdad R. Majlessi

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  C12M3/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6837 ,  C12Q1/6834 ,  C12Q2565/525 ,  C12Q2565/501 ,  C12Q2525/301

    摘要： Methods of the invention separate a target nucleic acid from a sample by using at least one capture probe oligonucleotide that contains a target-complementary region and a member of a specific binding pair that attaches the target nucleic acid to an immobilized probe on a capture support, thus forming a capture hybrid that is separated from other sample components before the target nucleic acid is released from the capture support and hybridized to a detection probe to form a detection hybrid that produces a detectable signal that indicates the presence of the target nucleic acid in the sample. Compositions for practicing the methods of the invention include a capture probe oligonucleotide made up a target-complementary region sequence and a covalently linked capture region sequence that includes a member of a specific binding pair.

    摘要翻译： 本发明的方法通过使用至少一种包含靶互补区的捕获探针寡核苷酸和将靶核酸附着在捕获载体上的固定化探针上的特异性结合对的成员来分离目标核酸， 从而形成在靶核酸从捕获载体释放之前与其它样品组分分离的捕获杂交物，并与检测探针杂交以形成检测杂交体，其产生可检测信号，其指示靶核酸存在于 样品。 用于实施本发明方法的组合物包括构成靶互补区序列的捕获探针寡核苷酸和包含特异性结合对成员的共价连接的捕获区序列。

    DOUBLE-STRANDED PROBES FOR THE FLUORESCENT DETECTION OF NUCLEIC ACIDS
    27.
    发明申请
    DOUBLE-STRANDED PROBES FOR THE FLUORESCENT DETECTION OF NUCLEIC ACIDS 有权
    用于荧光检测核酸的双重探针

    公开(公告)号：US20110129824A1

    公开(公告)日：2011-06-02

    申请号：US12445099

    申请日：2007-10-12

    申请人： Typhaine Dagland ,  Francois Rieunier

    发明人： Typhaine Dagland ,  Francois Rieunier

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/706 ,  C12Q1/6818 ,  C12Q1/6844 ,  C12Q1/703 ,  Y02A50/59 ,  C12Q2565/525 ,  C12Q2537/161 ,  C12Q2525/161 ,  C12Q2561/12 ,  C12Q2561/113 ,  C12Q2531/113

    摘要： The present invention relates to a double-stranded probe intended for the fluorescent detection of at least one single-stranded or double-stranded target nucleic acid, comprising: —a first strand of formula X1-(L1)a-S1-S′1-(L′1)b-Y1 intended for the detection of a first strand of the target nucleic acid which comprises a sequence of formula T1-T1; —a second strand of formula X2-(L2)c-S2-S′2-(L′2)d-Y2 intended for the detection of a second strand of the target nucleic acid, if present, said second strand of the target nucleic acid comprising a sequence of formula T2-T2; wherein two of X-1, X2, Y1, and Y2 represent a fluorescent donor, while the two others represent a fluorescent acceptor, and X1 and Y2 can not both represent a fluorescent donor.

    摘要翻译： 本发明涉及用于荧光检测至少一种单链或双链靶核酸的双链探针，包括： - 式X1-（L1）a-S1-S'1的第一链 - （L'1）b-Y1，其用于检测包含式T1-T1的序列的靶核酸的第一链; - 式X2-（L2）c-S2-S'2-（L'2）d-Y2的第二链，其用于检测靶核酸的第二链（如果存在），靶的第二链 包含式T2-T2的序列的核酸; 其中X-1，X2，Y1和Y2中的两个表示荧光供体，而另外两个表示荧光受体，X1和Y2不能都表示荧光供体。