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公开(公告)号：US11028125B2
公开(公告)日：2021-06-08
申请号：US16072175
申请日：2017-08-10
IPC分类号： C07K1/36 , C07K1/22 , C07K1/34 , C07K1/16 , C07K1/30 , B01D15/36 , B01D61/02 , B01D61/14 , C07K14/81 , C07K16/06 , A61K38/48 , A61K38/36 , A61K35/14 , A61K38/38 , A61K38/37 , A61K38/57 , B01D15/38
摘要： Methods of producing multiple protein products from blood-based materials including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins are described herein. The inventive methods include steps of: salt fractionation, chromatography, ultrafiltration, diafiltration, solvent-detergent treatment, and sterile filtration. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields. The sequence of process steps can be selected to obtain multiple products from various in-process materials, such as supernatants, pastes, chromatography flow-though, and chromatography washes.
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公开(公告)号：US20210087225A1
公开(公告)日：2021-03-25
申请号：US17067657
申请日：2020-10-10
发明人： Eugene ZURLO , Dennis CURTIN , Peter RADTKE , Kurt L. BRILLHART
摘要： Compositions of the inventive concept provide a therapeutic protein with less than 2% contamination by the therapeutic protein in denatured form. Such compositions provide enhanced specific activity and improved stability on storage and/or in serum than corresponding therapeutic protein preparations resulting from conventional isolation methods.
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公开(公告)号：US10954290B2
公开(公告)日：2021-03-23
申请号：US16029781
申请日：2018-07-09
申请人： BIOTEST AG
发明人： Wolfgang Möller , Dieter Rudnick , Oliver Maneg , Michael Rodemer , Matthias Germer , Veit Braun
IPC分类号： C07K16/12 , A61K39/395 , A61K47/18 , C07K16/06 , A61K9/00 , C07K1/30 , A61K39/00 , C07K16/14
摘要： An antibody preparation suitable for intravenous administration in humans includes IgG, IgA and at least 5% IgM antibodies by weight of the total amount of antibodies. The preparation is prepared from human plasma, has specific complement activating activity, and, in an in vitro assay with human serum suitable to determine the ability of the antibody preparation to activate complement unspecifically, the antibody preparation generates substantially no C5a and/or substantially no C3a. The antibody preparation can have medical uses.
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公开(公告)号：US10899792B2
公开(公告)日：2021-01-26
申请号：US16324280
申请日：2017-08-10
申请人： Spiber Inc.
发明人： Toshiaki Osawa , Yuya Sato , Keisuke Morita
摘要： An object of the present invention is to provide a method for efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate.
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公开(公告)号：US10800799B2
公开(公告)日：2020-10-13
申请号：US15941338
申请日：2018-03-30
发明人： Pamela C. Iraneta , Thomas H. Walter , Xin Zhang , Daniel P. Walsh , Kevin D. Wyndham , Raymond P. Fisk
IPC分类号： B01D15/00 , B01J20/00 , G01N1/00 , C07F9/10 , B01D15/32 , B01J20/28 , B01J20/287 , B01J20/32 , B01J20/10 , B01D15/22 , B01L3/00 , C07K1/30 , G01N30/88
摘要： Methods, kits and devices for separating phospholipids and proteins from small molecules in biochemical samples can feature an apparatus having a wetting barrier, at least one frit and a separation media. For example, an apparatus can include at least one wall defining a chamber having an exit and an entrance; a wetting barrier disposed between the exit and entrance, so as to define a separation media space located between the wetting barrier and the exit and a sample receiving area located between the wetting barrier and the entrance; and a separation media disposed adjacent to the wetting barrier and having a specific affinity for phospholipids.
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26.发明申请公开(公告)号：US20200270624A1
公开(公告)日：2020-08-27
申请号：US16611966
申请日：2018-05-10
申请人： BIOAPPLICATIONS INC.
发明人： Inhwan Hwang , Eun Ju Sohn , Yongjik Lee
摘要： The present invention relates to a recombinant vector carrying cellulose-binding domain 3 and a method for separating a target protein using the recombinant vector. The protein isolating method of the present invention can easily separate a fusion protein containing a target protein by using the recombinant vector to bind a transformed plant body to cellulose and can effectively isolate the target protein from a cellulose-binding domain by treating the fusion protein with enterokinase, thus being expected to find industrial applications in various fields.
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公开(公告)号：US20200263117A1
公开(公告)日：2020-08-20
申请号：US16791841
申请日：2020-02-14
申请人： Ecolab USA Inc.
发明人： Lan Xiao
摘要： Provided is a method of processing stillage from an ethanol production process. The method comprises treating stillage comprising oil, protein, and water upstream of a separation, concentration or evaporation step with at least one coagulant and at least one flocculant, thereby forming treated thin stillage comprising solids which include at least a portion of the oil and protein; and clarifying the treated stillage via a solid/liquid separation process thereby forming clarified stillage and a separated solids phase comprising at least a portion of the solids from the treated stillage.
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公开(公告)号：US20200231655A1
公开(公告)日：2020-07-23
申请号：US16721317
申请日：2019-12-19
摘要： Herein is reported a method for purifying cell cultivation supernatants either directly after fermentation or after one or more preliminary purification steps, such as protein A affinity chromatography. By adjusting the pH value in the acid range and subsequent incubation of the acidified solution host cell nucleic acid and host cell protein can be precipitated but the target polypeptide remains in solution. Thereafter the precipitate and therewith the contaminating host cell components can be removed by a simple physical separation step.
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29.发明申请公开(公告)号：US20200223886A1
公开(公告)日：2020-07-16
申请号：US16650772
申请日：2018-09-19
摘要： The present invention describes the integration of preparative crystallization, crystal separation, crystal washing and freeze-drying processes into single continuous process using pressure filtration. The process facilitates time reduction, and outlines the novel design of using multiple organic solvent washes and nitrogen gas purging for the removal of imbibed water and achieve final drug substance that meets the quality specifications.
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公开(公告)号：US10696734B2
公开(公告)日：2020-06-30
申请号：US15249811
申请日：2016-08-29
IPC分类号： C07K16/00 , C07K16/18 , C07K1/30 , A23K20/147 , A23K50/20 , A23K50/10 , A23K50/75 , A23K50/30 , A23K50/40 , A23K50/80 , A23L33/17 , A61K39/00
摘要： Described herein is secretory IgA isolated from the intestinal luminal fluid and intestinal mucosal of animals such as pigs and cows. Also included are methods of isolating secretory IgA. The secretory IgA is useful in food compositions such as animal and human food compositions as well as pharmaceutical compositions to increase growth rate, improve feed efficiency, reduce gastrointestinal inflammation, reduce a risk of gastrointestinal infection in the animal, or a combination thereof.
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