中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

385 records (took 0.097 seconds)

    ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL
    321.
    发明申请
    ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL 有权

    公开(公告)号：US20220290192A1

    公开(公告)日：2022-09-15

    申请号：US17507587

    申请日：2021-10-21

    Applicant: Genomatica, Inc.

    Inventor： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

    IPC: C12P7/18 ,  C12N15/52 ,  C12N9/04 ,  C07C29/80 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88

    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4-hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2-one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

    Methanol dehydrogenase fusion proteins
    323.
    发明授权
    Methanol dehydrogenase fusion proteins 有权

    公开(公告)号：US11441128B2

    公开(公告)日：2022-09-13

    申请号：US15771973

    申请日：2016-10-27

    Applicant: Genomatica, Inc.

    Inventor： Nelson R. Barton ,  Jingyi Li ,  Joseph R. Warner ,  Priti Pharkya

    IPC: C12N9/00 ,  C12N9/10 ,  C12N9/90 ,  C12N9/88 ,  C12N15/52 ,  C12N9/04 ,  C12P7/24

    Abstract: Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell. Also described are engineered cells expressing the fusion protein, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

    Aldehyde dehydrogenase variants and methods of use
    328.
    发明授权
    Aldehyde dehydrogenase variants and methods of use 有权

    公开(公告)号：US11299716B2

    公开(公告)日：2022-04-12

    申请号：US16499531

    申请日：2018-03-29

    Applicant: Genomatica, Inc.

    Inventor： Kevin Hoff ,  Cara Ann Tracewell ,  Kui Chan ,  Amit Shah ,  Joseph Warner ,  Michael Noble

    IPC: C12N9/02

    Abstract: The invention provides polypeptides and encoding nucleic acids of aldehyde dehydrogenase variants. The invention also provides cells expressing aldehyde dehydrogenase variants. The invention further provides methods for producing 3-hydroxybutyraldehyde (3-HBal) and/or 1,3-butanediol (1,3-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells. The invention additional provides methods for producing 4-hydroxybutyraldehyde (4-HBal) and/or 1,4-butanediol (1,4-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells.

Patent Agency Ranking