公开(公告)号：US20210147883A1

公开(公告)日：2021-05-20

申请号：US16785510

申请日：2020-02-07

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Priti Pharkya ,  Tae Hoon Yang ,  Jungik Choi

IPC: C12P7/18 ,  C12N9/02 ,  C12N9/12 ,  C12N9/16 ,  C12N15/52 ,  C12N1/20 ,  C12N9/00 ,  C07K14/245 ,  C12P7/42 ,  C12N9/52 ,  C12N9/88

Abstract: The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.

公开(公告)号：US20210108232A1

公开(公告)日：2021-04-15

申请号：US16884976

申请日：2020-05-27

Applicant: Genomatica, Inc.

Inventor： Jun Sun ,  Anthony P. Burgard ,  Priti Pharkya

IPC: C12P7/04 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12P7/02 ,  C12N15/52

Abstract: The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.

公开(公告)号：US20210071212A1

公开(公告)日：2021-03-11

申请号：US16915454

申请日：2020-06-29

Applicant: Genomatica, Inc.

Inventor： Andreas W. Schirmer ,  Haibo Wang ,  Stephen B. Del Cardayre ,  Zhihao Hu ,  Louis G. Hom ,  Baolong Zhu ,  Cindy Chang ,  Emanuela E. Popova

IPC: C12P7/64 ,  C12N9/02 ,  C12P7/42 ,  C12N15/62 ,  C12P7/18 ,  C12N15/52 ,  C12N9/04

Abstract: The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.

公开(公告)号：US20210040511A1

公开(公告)日：2021-02-11

申请号：US16997704

申请日：2020-08-19

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P7/42 ,  C12P7/40 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90

Abstract: The invention provides a non-naturally occurring microbial organism having a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.

公开(公告)号：US10711289B2

公开(公告)日：2020-07-14

申请号：US15538448

申请日：2015-12-22

Applicant: Genomatica, Inc.

Inventor： Lauri H. Suominen ,  Connor J. Galleher ,  Michael Japs ,  Mark J. Burk ,  Cara Tracewell

IPC: C12P13/00 ,  C12N11/16 ,  C12N9/96 ,  C12N9/88 ,  C12N1/20 ,  C12N15/52 ,  C12N1/02

Abstract: Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.

公开(公告)号：US10711288B2

公开(公告)日：2020-07-14

申请号：US14897285

申请日：2014-06-16

Applicant: GENOMATICA, INC.

Inventor： Andreas W. Schirmer ,  Haibo Wang ,  Stephen B. Del Cardayre ,  Zhihao Hu ,  Louis G. Hom ,  Baolong Zhu ,  Cindy Chang ,  Emanuela E. Popova

IPC: C12P7/64 ,  C12N1/21 ,  C12N9/04 ,  C12N9/02 ,  C12N9/88 ,  C12N9/10 ,  C12N9/06 ,  C12N15/53 ,  C12N15/54 ,  C12N9/60 ,  C07K19/00 ,  C12P7/42 ,  C12N15/62 ,  C12P7/18 ,  C12N15/52

Abstract: The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.

公开(公告)号：US10662451B2

公开(公告)日：2020-05-26

申请号：US15971878

申请日：2018-05-04

Applicant: Genomatica, Inc.

Inventor： Jun Sun ,  Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Wei Niu ,  John D. Trawick ,  Robert Haselbeck

IPC: C12N1/20 ,  C12N1/14 ,  C12P17/04 ,  C12P7/18 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms comprising 1,4-butanediol (14-BDO) and gamma-butyrolactone (GBL) pathways comprising at least one exogenous nucleic acid encoding a 14-BDO and GBL pathway enzyme expressed in a sufficient amount to produce 14-BDO and GBL. The invention additionally provides methods of using such microbial organisms to produce 14-BDO and GBL.

公开(公告)号：US10662136B2

公开(公告)日：2020-05-26

申请号：US15975630

申请日：2018-05-09

Applicant: Genomatica, Inc.

Inventor： Warren Clark ,  Michael Japs ,  Mark J. Burk

IPC: C07C29/76 ,  C12P7/18

Abstract: A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4-BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO.

公开(公告)号：US20190330670A1

公开(公告)日：2019-10-31

申请号：US16213671

申请日：2018-12-07

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P17/10 ,  C12P13/02 ,  C12P13/00 ,  C12P17/12 ,  C12P7/40 ,  C12N15/52 ,  C12N1/38 ,  C12P7/44

Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

公开(公告)号：US10435723B2

公开(公告)日：2019-10-08

申请号：US15140384

申请日：2016-04-27

Applicant: Genomatica, Inc.

Inventor： Liang Wu ,  Axel Christoph Trefzer ,  Stefaan Marie Andre De Wildeman ,  Marco Alexander Van Den Berg

IPC: C12P7/44 ,  C12P7/62 ,  C12P17/10 ,  C12N15/70

Abstract: The present invention relates to a method for preparing an adipate ester or thioester. The invention further relates to a method for preparing adipic acid from said ester or thioester. Further the invention provides a number of methods for preparing an intermediate for said ester or thioester. Further the invention relates to a method for preparing 6-amino caproic acid (6-ACA), a method for preparing 5-formyl valeric acid (5-FVA), and a method for preparing caprolactam. Further, the invention relates to a host cell for use in a method according to the invention.