公开(公告)号：US11034937B2

公开(公告)日：2021-06-15

申请号：US16414550

申请日：2019-05-16

申请人： Genomatica, Inc.

发明人： Derek L. Greenfield ,  Elizabeth J. Clarke ,  Eli S. Groban ,  Vikranth Arlagadda ,  Sungwon Lee ,  Xuezhi Li ,  Zhihao Hu ,  Baolong Zhu

IPC分类号： C12P7/04 ,  C12N9/02 ,  C12P7/64

摘要： The disclosure relates to variant carboxylic acid reductase (CAR) enzymes for the improved production of fatty alcohols in recombinant host cells.

公开(公告)号：US11981952B2

公开(公告)日：2024-05-14

申请号：US16915454

申请日：2020-06-29

申请人： Genomatica, Inc.

发明人： Andreas W. Schirmer ,  Haibo Wang ,  Stephen B. Del Cardayre ,  Zhihao Hu ,  Louis G. Hom ,  Baolong Zhu ,  Cindy Chang ,  Emanuela E. Popova

IPC分类号： C12P7/6409 ,  C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N15/52 ,  C12N15/62 ,  C12P7/18 ,  C12P7/42 ,  C12P7/6436

CPC分类号： C12P7/6409 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0071 ,  C12N9/0077 ,  C12N15/52 ,  C12N15/62 ,  C12P7/18 ,  C12P7/42 ,  C12P7/6436 ,  C12Y114/14001 ,  C12Y114/15003 ,  C07K2319/00 ,  C12Y101/00 ,  C12Y102/01042 ,  Y02E50/10

摘要： The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.

公开(公告)号：US11046635B2

公开(公告)日：2021-06-29

申请号：US15954451

申请日：2018-04-16

申请人： GENOMATICA, INC.

发明人： Zhihao Hu ,  Fernando Valle

IPC分类号： C07C69/533 ,  C12N1/20 ,  C12N1/16 ,  C12N1/14 ,  C12N1/12 ,  C07C69/34 ,  C07C69/52 ,  C10L1/02 ,  C10L1/19 ,  C11C3/00 ,  C12P7/64 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/00

摘要： Genetically engineered cells and microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use. The products are particularly useful as biofuels.

公开(公告)号：US20240150774A1

公开(公告)日：2024-05-09

申请号：US18344219

申请日：2023-06-29

申请人： Genomatica, Inc.

发明人： Derek L. GREENFIELD ,  Andreas W. Schirmer ,  Elizabeth J. Clarke ,  Eli S. Groban ,  Bernardo M. Da Costa ,  Zhihao Hu ,  Kevin Holden ,  Noah Helman

IPC分类号： C12N15/70 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12P7/04 ,  C12P7/6409 ,  C12P7/6436

CPC分类号： C12N15/70 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1288 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12P7/04 ,  C12P7/6409 ,  C12P7/6436 ,  C12Y101/011 ,  C12Y103/01009 ,  C12Y103/0101 ,  C12Y203/01039 ,  C12Y203/01041 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y207/08007 ,  C12Y402/01059 ,  C12Y503/03014 ,  C12Y604/01002

摘要： The disclosure relates to recombinant host cells including strain modifications effective to improve titer, yield and/or productivity of fatty acid derivatives. The disclosure further relates to cell cultures including the recombinant host cells for the fermentative production of fatty acid derivatives and compositions thereof.

公开(公告)号：US11359216B2

公开(公告)日：2022-06-14

申请号：US16889211

申请日：2020-06-01

申请人： Genomatica, Inc.

发明人： Andreas W. Schirmer ,  Noah Helman ,  Haibo Wang ,  Zhihao Hu ,  Vikranth Arlagadda ,  Alma Itzel Ramos-Solis ,  Elizabeth Clarke

IPC分类号： C12P7/18 ,  C12N9/00 ,  C12N9/16 ,  C12N15/52

摘要： The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.

公开(公告)号：US10961553B2

公开(公告)日：2021-03-30

申请号：US15888222

申请日：2018-02-05

申请人： GENOMATICA, INC.

发明人： Zhihao Hu ,  Vikranth Arlagadda

IPC分类号： C12P7/64 ,  C12N1/20 ,  C12N9/02 ,  B21D51/26 ,  C12P7/04 ,  A61K38/00 ,  A61K31/045 ,  C12R1/01

摘要： Methods and compositions, including nucleotide sequences, amino acid sequences, and host cells, for producing fatty alcohols are described.

公开(公告)号：US11021695B2

公开(公告)日：2021-06-01

申请号：US15958229

申请日：2018-04-20

申请人： GENOMATICA, INC.

发明人： Louis Hom ,  Na Trinh ,  Murtaza Alibhai ,  Zhihao Hu ,  Eli Groban ,  Vikranth Arlagadda ,  Elizabeth Clarke

IPC分类号： C12N9/16 ,  C12N15/00 ,  C12N9/18 ,  C12N15/63 ,  C10L1/02 ,  C07H21/04 ,  C12P21/06

摘要： The present invention relates to novel mutant thioesterase enzymes and naturally-occurring equivalents thereof, compositions made from such enzymes and uses of thioesterase enzymes. In particular, the present invention provides mutant thioesterase enzymes that have altered properties, for example, altered substrate specificity, altered activity, altered selectivity, and/or altered proportional yields in the product mixtures. The present invention also provides polynucleotides encoding such mutant thioesterase enzymes, and vectors and host cells comprising such polynucleotides. The invention further provides for novel uses of thioesterases in the production of various fatty acid derivatives, which are useful as, or as components of, industrial chemicals and fuels.

公开(公告)号：US20210071212A1

公开(公告)日：2021-03-11

申请号：US16915454

申请日：2020-06-29

申请人： Genomatica, Inc.

发明人： Andreas W. Schirmer ,  Haibo Wang ,  Stephen B. Del Cardayre ,  Zhihao Hu ,  Louis G. Hom ,  Baolong Zhu ,  Cindy Chang ,  Emanuela E. Popova

IPC分类号： C12P7/64 ,  C12N9/02 ,  C12P7/42 ,  C12N15/62 ,  C12P7/18 ,  C12N15/52 ,  C12N9/04

摘要： The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.

公开(公告)号：US10711288B2

公开(公告)日：2020-07-14

申请号：US14897285

申请日：2014-06-16

申请人： GENOMATICA, INC.

发明人： Andreas W. Schirmer ,  Haibo Wang ,  Stephen B. Del Cardayre ,  Zhihao Hu ,  Louis G. Hom ,  Baolong Zhu ,  Cindy Chang ,  Emanuela E. Popova

IPC分类号： C12P7/64 ,  C12N1/21 ,  C12N9/04 ,  C12N9/02 ,  C12N9/88 ,  C12N9/10 ,  C12N9/06 ,  C12N15/53 ,  C12N15/54 ,  C12N9/60 ,  C07K19/00 ,  C12P7/42 ,  C12N15/62 ,  C12P7/18 ,  C12N15/52

摘要： The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.

公开(公告)号：US11060099B2

公开(公告)日：2021-07-13

申请号：US15650285

申请日：2017-07-14

申请人： Genomatica, Inc.

发明人： Derek L. Greenfield ,  Andreas W. Schirmer ,  Elizabeth J. Clarke ,  Eli S. Groban ,  Bernardo M. Da Costa ,  Zhihao Hu ,  Kevin Holden ,  Noah Helman

IPC分类号： C12N1/21 ,  C12P7/64 ,  C07K14/195 ,  C07K14/245 ,  C12N9/16 ,  C12N15/70 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12N9/00 ,  C12P7/04

摘要： The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, recombinant host cells and recombinant host cell cultures engineered to produce fatty acid derivative compositions comprising fatty acids, fatty alcohols, fatty aldehydes, fatty esters, alkanes, terminal olefins, internal olefins or ketones. The fatty acid derivative composition is produced extracellularly with a higher titer, yield or productivity than the corresponding wild type or non-engineered host cell.