公开(公告)号：US10435723B2

公开(公告)日：2019-10-08

申请号：US15140384

申请日：2016-04-27

Applicant: Genomatica, Inc.

Inventor： Liang Wu ,  Axel Christoph Trefzer ,  Stefaan Marie Andre De Wildeman ,  Marco Alexander Van Den Berg

IPC: C12P7/44 ,  C12P7/62 ,  C12P17/10 ,  C12N15/70

Abstract: The present invention relates to a method for preparing an adipate ester or thioester. The invention further relates to a method for preparing adipic acid from said ester or thioester. Further the invention provides a number of methods for preparing an intermediate for said ester or thioester. Further the invention relates to a method for preparing 6-amino caproic acid (6-ACA), a method for preparing 5-formyl valeric acid (5-FVA), and a method for preparing caprolactam. Further, the invention relates to a host cell for use in a method according to the invention.

公开(公告)号：US10273508B2

公开(公告)日：2019-04-30

申请号：US15148759

申请日：2016-05-06

Applicant: Genomatica, Inc.

Inventor： Stephen J. Van Dien ,  Anthony P. Burgard ,  Robert Haselbeck ,  Catherine J. Pujol-Baxley ,  Wei Niu ,  John D. Trawick ,  Harry Yim ,  Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun

IPC: C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12P7/18 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.

公开(公告)号：US10227616B2

公开(公告)日：2019-03-12

申请号：US15303931

申请日：2015-04-14

Applicant: Novamont S.p.A. ,  Genomatica, Inc.

Inventor： Maria Dani ,  Giuseppe Ruggiero ,  Davide Perini ,  Alice Bianchi

IPC: C12P7/18

Abstract: Process for the synthesis of 1,4-butanediol comprising fermentation of a culture medium by a microorganism provided with at least one metabolic pathway for the synthesis of 1,4-butanediol and able to use saccharose, in which said culture medium comprises a glucose and saccharose mixture comprising, based on the combined weight of glucose and saccharose, 10-90% by weight of saccharose and 10-90% by weight of glucose.

公开(公告)号：US10006055B2

公开(公告)日：2018-06-26

申请号：US14869872

申请日：2015-09-29

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

IPC: C12P7/04 ,  C12N15/52 ,  C12P5/02 ,  C12N15/70 ,  C12P7/16

CPC classification number: C12P7/04 ,  C12N15/52 ,  C12N15/70 ,  C12P5/026 ,  C12P7/16

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene or crotyl alcohol pathway. The invention additionally provides methods of using such organisms to produce butadiene or crotyl alcohol.

公开(公告)号：US09982281B2

公开(公告)日：2018-05-29

申请号：US14692630

申请日：2015-04-21

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

IPC: C12P7/18 ,  C12N15/52

CPC classification number: C12P7/18 ,  C12N15/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

公开(公告)号：US09909150B2

公开(公告)日：2018-03-06

申请号：US14071318

申请日：2013-11-04

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12N1/20 ,  C12N1/16 ,  C12N1/14 ,  C12P7/20 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N15/52

CPC classification number: C12P7/24 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/52 ,  C12P7/04 ,  C12P7/18 ,  C12P7/20 ,  C12Y101/01244 ,  C12Y105/0102

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol. Also provided herein are methods for using such an organism to produce 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol.

公开(公告)号：US09885064B2

公开(公告)日：2018-02-06

申请号：US14185709

申请日：2014-02-20

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Christophe H. Schilling ,  Anthony P. Burgard ,  John D. Trawick

IPC: C12N1/20 ,  C12N1/12 ,  C12P7/62 ,  C12P7/04 ,  C07H21/04 ,  C12P7/18 ,  C12N9/02 ,  C12N9/10 ,  C12N9/00 ,  C12P19/32 ,  C12N15/70

CPC classification number: C12P7/18 ,  C12N9/0008 ,  C12N9/0036 ,  C12N9/1007 ,  C12N9/93 ,  C12N15/70 ,  C12P19/32 ,  Y02E50/343

Abstract: The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA.

公开(公告)号：US09732361B2

公开(公告)日：2017-08-15

申请号：US14059131

申请日：2013-10-21

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Jun Sun ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C08F36/00 ,  C12P5/02 ,  C12N1/20 ,  C12N1/00 ,  C07C9/00 ,  C12N15/52 ,  C07C11/16 ,  C08F136/06

CPC classification number: C12P5/026 ,  C07C11/16 ,  C08F136/06 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.

公开(公告)号：US09663805B2

公开(公告)日：2017-05-30

申请号：US14105705

申请日：2013-12-13

Applicant: Genomatica, Inc

Inventor： Petronella Catharina Raemakers-Franken ,  Martin Schurmann ,  Axel Christoph Trefzer ,  Stefaan Marie Andre De Wildeman

IPC: C12P13/02 ,  C12P7/40 ,  C12P7/26 ,  C12P13/00 ,  C07C227/06 ,  C07D223/10 ,  C07D201/08

CPC classification number: C12P13/001 ,  C07C227/06 ,  C07D201/08 ,  C07D223/10 ,  C12P13/005 ,  C12P13/02 ,  C07C229/08

Abstract: The invention relates to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’) using a biocatalyst. The invention further relates to a method for preparing e-caprolactam (hereafter referred to as ‘caprolactam’) by cyclising such 6-ACA. The invention further relates to a host cell, a micro-organism, or a polynucleotide which may be used in the preparation of 6-ACA or caprolactam.

公开(公告)号：US20150368676A1

公开(公告)日：2015-12-24

申请号：US14603174

申请日：2015-01-22

Applicant: GENOMATICA, INC.

Inventor： Mark J. BURK ,  Stephen J. VAN DIEN ,  Anthony P. BURGARD ,  Wei NIU

IPC: C12P7/18 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12P7/42 ,  C12N9/88

CPC classification number: C12N15/52 ,  B01D3/002 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/88 ,  C12N9/93 ,  C12N15/70 ,  C12N15/81 ,  C12P7/18 ,  C12P7/42 ,  C12P7/52 ,  C12P17/04 ,  C12Y101/01061 ,  C12Y102/01076 ,  C12Y401/01071 ,  C12Y602/01004 ,  Y02P20/52

Abstract: The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO.

Abstract translation: 本发明提供了一种非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）生物合成途径的微生物生物，其具有至少一种编码4-羟基丁酸脱氢酶的外源核酸，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛 脱氢酶或α-酮戊二酸脱羧酶，其中外源核酸以足够的量表达以产生单体4-羟基丁酸（4-HB）。 还提供了非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）和1,4-丁二醇（BDO）生物合成途径的微生物生物，该途径包括至少一种编码4-羟基丁酸脱氢酶的外源核酸 ，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛脱氢酶，4-羟基丁酸酯：CoA转移酶，4-丁酸酯激酶，磷酸转移酶，α-酮戊二酸脱羧酶，醛脱氢酶，醇脱氢酶或醛/醇脱氢酶，其中外源核酸为 以足够的量表示产生1,4-丁二醇（BDO）。 另外提供了用于生产4-HB和BDO的方法。