公开(公告)号：US20170183694A1

公开(公告)日：2017-06-29

申请号：US15191421

申请日：2016-06-23

Applicant: Genomatica, Inc.

Inventor： Priti PHARKYA ,  Anthony P. BURGARD ,  Stephen J. VAN DIEN ,  Robin E. OSTERHOUT ,  Mark J. BURK ,  John D. TRAWICK ,  Michael P. KUCHINSKAS ,  Brian STEER ,  Stefan ANDRAE ,  Amit SHAH

IPC: C12P7/18 ,  C12P7/42 ,  C12N1/20 ,  C12P17/04 ,  C07C211/09 ,  C12P13/00 ,  C07C47/19 ,  C07C31/20 ,  C07D307/33 ,  C12N15/52 ,  C12N9/10

CPC classification number: C12P7/18 ,  C07C31/207 ,  C07C47/19 ,  C07C211/09 ,  C07D307/33 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/13 ,  C12N15/52 ,  C12P7/24 ,  C12P7/42 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P17/04 ,  C12P19/32 ,  C12Y208/03

Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.

公开(公告)号：US20160355846A1

公开(公告)日：2016-12-08

申请号：US15148759

申请日：2016-05-06

Applicant: Genomatica, Inc.

Inventor： Stephen J. VAN DIEN ,  Anthony P. BURGARD ,  Robert HASELBECK ,  Catherine J. PUJOL-BAXLEY ,  Wei NIU ,  John D. TRAWICK ,  Harry YIM ,  Mark J. BURK ,  Robin E. OSTERHOUT ,  Jun SUN

IPC: C12P7/18 ,  C12N9/10 ,  C12N15/52 ,  C12N9/16 ,  C12N9/02 ,  C12N9/04 ,  C12N9/88 ,  C12N9/00

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.

Abstract translation: 本发明提供了包含1,4-丁二醇（BDO）途径的非天然存在的微生物生物，其包含至少一种编码足够量的BDO途径酶的外源核酸以产生BDO并进一步优化用于BDO的表达。 本发明还提供了使用这种微生物生产BDO的方法。

公开(公告)号：US20150368676A1

公开(公告)日：2015-12-24

申请号：US14603174

申请日：2015-01-22

Applicant: GENOMATICA, INC.

Inventor： Mark J. BURK ,  Stephen J. VAN DIEN ,  Anthony P. BURGARD ,  Wei NIU

IPC: C12P7/18 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12P7/42 ,  C12N9/88

CPC classification number: C12N15/52 ,  B01D3/002 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/88 ,  C12N9/93 ,  C12N15/70 ,  C12N15/81 ,  C12P7/18 ,  C12P7/42 ,  C12P7/52 ,  C12P17/04 ,  C12Y101/01061 ,  C12Y102/01076 ,  C12Y401/01071 ,  C12Y602/01004 ,  Y02P20/52

Abstract: The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO.

Abstract translation: 本发明提供了一种非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）生物合成途径的微生物生物，其具有至少一种编码4-羟基丁酸脱氢酶的外源核酸，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛 脱氢酶或α-酮戊二酸脱羧酶，其中外源核酸以足够的量表达以产生单体4-羟基丁酸（4-HB）。 还提供了非天然存在的微生物生物催化剂，其包括具有4-羟基丁酸（4-HB）和1,4-丁二醇（BDO）生物合成途径的微生物生物，该途径包括至少一种编码4-羟基丁酸脱氢酶的外源核酸 ，琥珀酰辅酶A合成酶，CoA依赖性琥珀酸半醛脱氢酶，4-羟基丁酸酯：CoA转移酶，4-丁酸酯激酶，磷酸转移酶，α-酮戊二酸脱羧酶，醛脱氢酶，醇脱氢酶或醛/醇脱氢酶，其中外源核酸为 以足够的量表示产生1,4-丁二醇（BDO）。 另外提供了用于生产4-HB和BDO的方法。

公开(公告)号：US20140371417A1

公开(公告)日：2014-12-18

申请号：US14262461

申请日：2014-04-25

Applicant: Genomatica, Inc.

Inventor： Priti PHARKYA ,  Anthony P. BURGARD ,  Stephen J. VAN DIEN ,  Robin E. OSTERHOUT ,  Mark J. BURK ,  John D. TRAWICK ,  Michael P. KUCKINSKAS ,  Brian STEER

IPC: C12N9/04 ,  C12P17/04 ,  C12P13/00 ,  C12P19/32 ,  C12P7/18 ,  C12P7/62 ,  C12P7/24

CPC classification number: C12P7/18 ,  C07C31/207 ,  C07C47/19 ,  C07C211/09 ,  C07D307/33 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/13 ,  C12N15/52 ,  C12P7/24 ,  C12P7/42 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P17/04 ,  C12P19/32 ,  C12Y208/03

Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.

Abstract translation: 本发明提供具有4-羟基丁酸酯，γ-丁内酯，1,4-丁二醇，4-羟基丁醛，4-羟基丁酰辅酶A和/或腐胺途径的非天然存在的微生物，其能够产生4-羟基丁酸酯，其中 微生物生物体包含一种或多种遗传修饰。 本发明另外提供使用微生物生产4-羟基丁酸酯，γ-丁内酯，1,4-丁二醇，4-羟基丁醛，4-羟基丁酰-CoA和/或腐胺或相关产品的方法。

公开(公告)号：US20170362565A1

公开(公告)日：2017-12-21

申请号：US15585078

申请日：2017-05-02

Applicant: Genomatica, Inc.

Inventor： Priti PHARKYA ,  Anthony P. BURGARD ,  Stephen J. VAN DIEN ,  Robin E. OSTERHOUT ,  Mark J. BURK ,  John D. TRAWICK ,  Michael P. KUCHINSKAS ,  Brian STEER

IPC: C12N1/20 ,  C12P7/18 ,  C12N15/63 ,  C12N15/52 ,  C12N9/18 ,  C07C31/20 ,  C08G63/181 ,  C08G63/16 ,  C08G63/06 ,  C08G18/32 ,  C08G18/28 ,  C07C59/01 ,  C12P7/42 ,  C08G69/02

CPC classification number: C12N1/20 ,  C07C31/207 ,  C07C59/01 ,  C08G18/284 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/16 ,  C08G63/181 ,  C08G69/02 ,  C12N9/18 ,  C12N15/52 ,  C12N15/63 ,  C12P7/18 ,  C12P7/42 ,  C12Y301/01 ,  Y02P20/52

Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms.

公开(公告)号：US20170247709A1

公开(公告)日：2017-08-31

申请号：US15290895

申请日：2016-10-11

Applicant: Genomatica, Inc.

Inventor： Anthony P. BURGARD ,  Mark J. BURK ,  Robin E. OSTERHOUT ,  Priti PHARKYA ,  Jingyi LI

IPC: C12N15/52 ,  C12P7/18 ,  C12N1/20 ,  C12N1/16 ,  C12N1/14

CPC classification number: C12N15/52 ,  C08G63/16 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12P7/18 ,  C12P19/32

Abstract: Provided herein are non-naturally occurring eukaryotic organisms that can be engineered to produce and increase the availability of cytosolic acetyl-CoA. Also provided herein are non-naturally occurring eukaryotic organisms having a 1,3-butanediol (1,3-BDO) pathway. and methods of using such organisms to produce 1,3-BDO.

公开(公告)号：US20170191085A1

公开(公告)日：2017-07-06

申请号：US15325396

申请日：2015-07-02

Applicant: Genomatica, Inc.

Inventor： Priti PHARKYA ,  Anthony P. BURGARD ,  Mark J. BURK

IPC: C12P5/02 ,  C12N9/02 ,  C12N9/12 ,  C12N9/04 ,  C08F36/14 ,  C12P3/00 ,  C12P7/40 ,  C01B3/00 ,  C08F36/06 ,  C12N15/52 ,  C12N9/10

CPC classification number: C12P5/026 ,  C01B3/00 ,  C08F36/06 ,  C08F36/14 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0067 ,  C12N9/10 ,  C12N9/12 ,  C12N15/52 ,  C12P3/00 ,  C12P7/40 ,  C12Y101/02007 ,  C12Y101/03013 ,  C12Y102/99003 ,  Y02E50/343 ,  Y02E60/324

Abstract: The invention provides non-naturally occurring microbial organisms containing butadiene or 2,4-pentadienoate pathways comprising at least one exogenous nucleic acid encoding a butadiene or 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce butadiene or 2,4-pentadienoate. The organism can further contain a hydrogen synthesis pathway. The invention additionally provides methods of using such microbial organisms to produce butadiene or 2,4-pentadienoate by culturing a non-naturally occurring microbial organism containing butadiene or 2,4-pentadienoate pathways as described herein under conditions and for a sufficient period of time to produce butadiene or 2,4-pentadienoate. Hydrogen can be produced together with the production of butadiene or 2,4-pentadienoate.

公开(公告)号：US20170044572A1

公开(公告)日：2017-02-16

申请号：US15078437

申请日：2016-03-23

Applicant: Genomatica, Inc.

Inventor： Mark J. BURK ,  Anthony P. BURGARD ,  Jun SUN ,  Robin E. OSTERHOUT ,  Priti PHARKYA

IPC: C12P5/02 ,  C12N15/52

CPC classification number: C12P5/02 ,  C07C1/24 ,  C07C2521/04 ,  C07C2521/12 ,  C07C2521/16 ,  C07C2523/10 ,  C07C2523/28 ,  C07C2523/31 ,  C07C2523/745 ,  C07C2527/167 ,  C07C2527/173 ,  C12N15/52 ,  C12P5/026 ,  C12P7/04 ,  Y02E50/343 ,  C07C11/167

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.

Abstract translation: 本发明提供具有丁二烯途径的非天然存在的微生物。 本发明另外提供了使用这些生物体来生产丁二烯的方法。

公开(公告)号：US20160319313A1

公开(公告)日：2016-11-03

申请号：US14954487

申请日：2015-11-30

Applicant: Genomatica, Inc.

Inventor： Jun SUN ,  Mark J. BURK ,  Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Wei NIU ,  John D. TRAWICK ,  Robert HASELBECK

IPC: C12P17/04 ,  C12N15/52 ,  C12P7/18

CPC classification number: C12P17/04 ,  C12N1/20 ,  C12N15/52 ,  C12P7/18

Abstract: The invention provides non-naturally occurring microbial organisms comprising 1,4-butanediol (14-BDO) and gamma-butyrolactone (GBL) pathways comprising at least one exogenous nucleic acid encoding a 14-BDO and GBL pathway enzyme expressed in a sufficient amount to produce 14-BDO and GBL. The invention additionally provides methods of using such microbial organisms to produce 14-BDO and GBL.

Abstract translation: 本发明提供包含1,4-丁二醇（14-BDO）和γ-丁内酯（GBL）途径的非天然存在的微生物生物，其包含至少一种编码14-BDO和GBL途径酶的外源核酸，其以足够的量表达， 生产14-BDO和GBL。 本发明另外提供了使用这种微生物生物来生产14-BDO和GBL的方法。

公开(公告)号：US20240150798A1

公开(公告)日：2024-05-09

申请号：US18482834

申请日：2023-10-06

Applicant: Genomatica, Inc.

Inventor： Benjamin ADELSTEIN ,  Robin E. OSTERHOUT ,  Mark J. BURK

IPC: C12P7/62 ,  A23L33/135 ,  A61K31/22 ,  A61K35/66 ,  A61K36/064 ,  C07C69/675 ,  C12N1/00 ,  C12N1/14 ,  C12N1/20 ,  C12N9/18 ,  C12N15/52

CPC classification number: C12P7/62 ,  A23L33/135 ,  A61K31/22 ,  A61K35/66 ,  A61K36/064 ,  C07C69/675 ,  C12N1/00 ,  C12N1/145 ,  C12N1/205 ,  C12N9/18 ,  C12N15/52 ,  C12Y101/01035 ,  C12Y101/011 ,  C12Y301/01067 ,  C12Y301/08001 ,  C07B2200/05 ,  C12R2001/00 ,  Y02P20/10

Abstract: Provided herein are non-naturally occurring microbial organisms having a pathway for production of (3R)-hydroxybutyl (3R)-hydroxybutyrate, wherein the organism can further include a (R)-1,3-butanediol pathway, a (3R)-hydroxybutyrate pathway, a (3R)-hydroxybutyryl-CoA pathway, an acetoacetate pathway, an acetoacetyl-CoA pathway, a (3R)-hydroxybutyl-ACP pathway, or an acetoacetyl-ACP pathway. Additionally provided are methods and processes for producing and isolating (3R)-hydroxybutyl (3R)-hydroxybutyrate using the microbial organisms, and various compositions having the (3R)-hydroxybutyl (3R)-hydroxybutyrate. Still further provided are methods of treating or preventing a disease, disorder or condition using the (3R)-hydroxybutyl (3R)-hydroxybutyrate produced by the microbial organisms of the invention.