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45 条记录 (耗时 0.061 秒)

    Method for producing dimer alkaloids
    31.
    发明授权
    Method for producing dimer alkaloids 失效
    二聚生物碱的生产方法

    公开(公告)号：US4279817A

    公开(公告)日：1981-07-21

    申请号：US582373

    申请日：1975-05-30

    申请人： James P. Kutney

    发明人： James P. Kutney

    IPC分类号： C07D471/20 ,  C07D519/04

    CPC分类号： C07D519/04 ,  Y10S514/908

    摘要： A concerted process for the synthesis of a dimer consisting of an indole unit and a dihydroindole unit possessing natural stereochemistry which comprises:(a) forming an N-oxide intermediate from said indole unit;(b) treating said N-oxide indole intermediate in the presence of acetic anhydride or halogenated derivative thereof to effect a Polonovski-type fragmentation reaction;(c) without isolating the N-oxide indole intermediate and at a temperature of about -10.degree. C. to +10.degree. C., coupling said reaction product with a dihydroindole unit in the presence of acetic anhydride or a halogenated derivative thereof at a low temperature of about -10.degree. C. to +10.degree. C. under inert conditions; and(d) subsequently reducing the immonium nitrogen on the indole unit by reacting with aqueous alkali metal borohydride to produce a dimer.

    摘要翻译： 一种由具有天然立体化学的吲哚单元和二氢吲哚单元组成的二聚体的合成方法，其包括：（a）从所述吲哚单元形成N-氧化物中间体; （b）在乙酸酐或其卤化衍生物的存在下处理所述N-氧化物吲哚中间体以进行Polonovski型断裂反应; （c）在不分离N-氧化物吲哚中间体的情况下，在约-10℃至+ 10℃的温度下，在乙酸酐或其卤代衍生物的存在下，将所述反应产物与二氢吲哚单元偶合 低温约-10℃至+10℃，在惰性条件下; 和（d）随后通过与碱金属硼氢化钠水溶液反应产生二聚体来还原吲哚单元上的亚硝酸盐。

    Methods for detecting cervical cancer
    34.
    发明授权
    Methods for detecting cervical cancer 失效
    检测宫颈癌的方法

    公开(公告)号：US5919624A

    公开(公告)日：1999-07-06

    申请号：US781424

    申请日：1997-01-10

    申请人： Thomas Ried ,  Gert Auer ,  Evelin Schrock ,  Kerstin Heselmeyer ,  Merryn Macville

    发明人： Thomas Ried ,  Gert Auer ,  Evelin Schrock ,  Kerstin Heselmeyer ,  Merryn Macville

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6886 ,  C12Q2600/112

    摘要： The invention provides a method of detecting the presence of invasive cervical carcinoma in a subject comprising detecting in a cervical cell from the subject the presence of a chromosome abnormality which is associated with invasive cervical carcinoma; the presence of the cervical cell containing the chromosome abnormality indicating the presence of invasive cervical carcinoma in the subject. The invention also provides a method of detecting the presence of advanced-stage cervical carcinoma in a subject comprising detecting in a cervical cell from the subject the presence of a chromosome abnormality associated with advanced-stage cervical carcinoma; the presence of the cervical cell containing the chromosome abnormality indicating the presence of advanced-stage cervical carcinoma in the subject. The invention also provides a method of classifying the progression of dysplastic cervical cells from a non-invasive cervical carcinoma to an invasive cervical carcinoma comprising analyzing the dysplastic cervical cells for the presence of a chromosome abnormality which is associated with invasive cervical carcinoma, and classifying the dysplastic cervical cells having the chromosome abnormality as having progressed from a non-invasive cervical carcinoma cells to an invasive cervical carcinoma. The invention further provides kits comprising nucleic acids that specifically hybridize to chromosome 3q and specifically hybridize to another chromosome, and to compositions comprising nucleic acids.

    摘要翻译： 本发明提供一种检测受试者侵袭性宫颈癌存在的方法，包括在受试者宫颈细胞中检测与侵入性宫颈癌相关的染色体异常的存在; 含有染色体异常的子宫颈细胞的存在表明受试者存在浸润性宫颈癌。 本发明还提供一种检测受试者中晚期宫颈癌存在的方法，包括在受试者的子宫颈细胞中检测与晚期宫颈癌相关的染色体异常的存在; 含有染色体异常的子宫颈细胞的存在表明该受试者存在晚期宫颈癌。 本发明还提供了将发育不良的子宫颈细胞从非侵入性宫颈癌分级到浸润性宫颈癌的方法，包括分析发育不良的宫颈细胞是否存在与侵入性宫颈癌相关的染色体异常，并将 具有染色体异常的发育不良的宫颈细胞从非侵入性宫颈癌细胞进展到浸润性宫颈癌。 本发明还提供了包含与染色体3q特异性杂交并与另一染色体特异性杂交的核酸以及包含核酸的组合物的试剂盒。

    Exchangeable template reaction
    37.
    发明授权
    Exchangeable template reaction 失效
    可交换模板反应

    公开(公告)号：US5503995A

    公开(公告)日：1996-04-02

    申请号：US261670

    申请日：1994-06-17

    申请人： Yury Khudyakov ,  Howard A. Fields

    发明人： Yury Khudyakov ,  Howard A. Fields

    IPC分类号： C12N15/09 ,  C07K14/02 ,  C07K14/18 ,  C12N15/10 ,  C12N15/36 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12N15/10 ,  C07K14/005 ,  C12Q1/6853 ,  C12N2730/10122 ,  C12N2770/24222

    摘要： The invention provides a method for the synthesis of DNA based on a cyclic mechanism of combining deoxyoligonucleotides comprising combining: (a) a series of unique single-stranded deoxypolynucleotides, each having a 5' sequence which, when in double-stranded form, can be enzymatically treated to form a unique 3' single-stranded protrusion for selective cyclic hybridization with another unique single-stranded deoxypolynucleotide of the series; (b) a unique deoxypolynucleotide having a 3' sequence which can selectively hybridize with one of the unique single-stranded deoxypolynucleotides of (a); (c) a polymerase which can direct the formation of double-stranded deoxypolynucleotides from the single-stranded deoxypolynucleotides; and (d) an enzyme which can form a unique single-stranded 3' protrusion from the double-stranded deoxypolynucleotides; under conditions which hybridize the unique single-stranded deoxypolynucleotides in a cyclic manner to form the DNA. Also provided is a kit comprising a series of unique synthesized single-stranded deoxypolynucleotides, each having a 5' sequence which, when in double-stranded form, can be enzymatically treated to form a unique 3' single-stranded protrusion for selective cyclic hybridization with another unique single-stranded deoxypolynucleotide of the series.

    摘要翻译： 本发明提供了一种基于组合脱氧寡核苷酸的循环机制合成DNA的方法，包括组合：（a）一系列独特的单链脱氧多核苷酸，每个具有5'序列，当双链形式可以是 酶处理以形成独特的3'单链突起，用于与该系列的另一种独特的单链脱氧多核苷酸进行选择性循环杂交; （b）具有3'序列的独特的脱氧多核苷酸，其可以与（a）的独特的单链脱氧核苷酸之一选择性杂交; （c）可以从单链脱氧寡核苷酸引导形成双链脱氧核苷酸的聚合酶; 和（d）可以从双链脱氧寡核苷酸形成独特的单链3'突起的酶; 在独特的单链脱氧核苷酸以循环方式杂交以形成DNA的条件下。 还提供了一种试剂盒，其包含一系列独特的合成的单链脱氧核苷酸，每个具有5'序列，当以双链形式时，可以进行酶处理以形成用于选择性环状杂交的独特的3'单链突起， 该系列的另一种独特的单链脱氧多核苷酸。

    Method to enhance the sensitivity of MRI for magnetic susceptibility effects
    39.
    发明授权
    Method to enhance the sensitivity of MRI for magnetic susceptibility effects 失效
    提高磁共振磁敏感性的方法

    公开(公告)号：US5459400A

    公开(公告)日：1995-10-17

    申请号：US106372

    申请日：1993-08-13

    申请人： Chrit T. W. Moonen

    发明人： Chrit T. W. Moonen

    IPC分类号： G01R33/20 ,  A61B5/055 ,  G01R33/48 ,  G01R33/561 ,  G01R33/563 ,  G01R33/565 ,  G01V3/00

    CPC分类号： G01R33/561 ,  G01R33/563 ,  G01R33/4806 ,  G01R33/56527

    摘要： A method for enhancing the sensitivity of gradient-recalled echo imaging for T2* effects in dynamic magnetic resonance imaging which involves the step of delaying gradient-recalled echoes so that the gradient-recalled echoes are subjected to magnetic susceptibility effects for an extended period of time. The gradient-recalled echoes are delayed beyond a subsequent radio frequency pulse by applying an additional gradient which dephases any gradient-recalled echo of spins that are excited in the radio frequency repetition time period in which said gradient-recalled echoes are produced.

    摘要翻译： 一种提高梯度回波成像对于动态磁共振成像中T2 *效应的灵敏度的方法，其涉及延迟梯度回波回波的步骤，使得梯度回波回波在延长的时间段内经受磁化率效应 。 梯度回波的回波被延迟超过随后的射频脉冲，通过应用额外的梯度，该梯度去除在产生所述梯度回波回波的射频重复时间段内被激发的任何梯度回波的自旋回波。

    Method to enhance the sensitivity of MRI for magnetic susceptibility effects
    40.
    发明授权
    Method to enhance the sensitivity of MRI for magnetic susceptibility effects 失效
    提高磁共振磁敏感性的方法

    公开(公告)号：US5300886A

    公开(公告)日：1994-04-05

    申请号：US841994

    申请日：1992-02-28

    申请人： Chrit T. W. Moonen

    发明人： Chrit T. W. Moonen

    IPC分类号： G01R33/20 ,  A61B5/055 ,  G01R33/48 ,  G01R33/561 ,  G01R33/563 ,  G01R33/565

    CPC分类号： G01R33/561 ,  G01R33/563 ,  G01R33/4806 ,  G01R33/56527

    摘要： A method for enhancing the sensitivity of gradient-recalled echo imaging for T2* effects in dynamic magnetic resonance imaging which involves the step of delaying gradient-recalled echoes so that the gradient-recalled echoes are subjected to magnetic susceptibility effects for an extended period of time. The gradient-recalled echoes are delayed beyond a subsequent radio frequency pulse by applying an additional gradient which dephases any gradient-recalled echo of spins that are excited in the radio frequency repetition time period in which said gradient-recalled echoes are produced.

    摘要翻译： 一种提高梯度回波成像对于动态磁共振成像中T2 *效应的灵敏度的方法，其涉及延迟梯度回波回波的步骤，使得梯度回波回波在延长的时间段内经受磁化率效应 。 梯度回波的回波被延迟超过随后的射频脉冲，通过应用额外的梯度，该梯度去除在产生所述梯度回波回波的射频重复时间段内被激发的任何梯度回波的自旋回波。