公开(公告)号：US20210207124A1

公开(公告)日：2021-07-08

申请号：US17211484

申请日：2021-03-24

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov SKOG ,  Leileata M. RUSSO

IPC: C12N15/10

Abstract: A method for extracting nucleic acids from a biological sample by isolating nucleic acid-containing particles from the biological sample by one or more centrifugation procedures, performing one or more steps to mitigate adverse factors that prevent or might prevent high quality nucleic acid extraction, and extracting nucleic acids from the isolated particles. The centrifugation procedures are performed at a speed not exceeding about 200,000 g. The extracted nucleic acids contain both 18S and 28S rRNA.

公开(公告)号：US10808240B2

公开(公告)日：2020-10-20

申请号：US16301176

申请日：2017-05-15

Applicant: Exosome Diagnostics, Inc.

Inventor： Georg Stoll ,  Daniel Enderle ,  Mikkel Noerholm ,  Johan Karl Olov Skog

IPC: C12N15/10 ,  C12N15/11 ,  C07H1/06

Abstract: The invention provides novel methods and kits for fully automated high-throughput method for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, novel methods and kits for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles that do not require the use of phenol or chloroform, and for extracting nucleic acids from the extracellular vesicles and/or from the biological samples.

公开(公告)号：US20200224245A1

公开(公告)日：2020-07-16

申请号：US16631603

申请日：2018-07-18

Applicant: Exosome Diagnostics, Inc.

Inventor： Robert KITCHEN ,  Michael VALENTINO ,  Johan SKOG ,  Vasisht TADIGOTLA ,  Dalin CHAN ,  Sudipto CHAKRABORTTY ,  James HURLEY

IPC: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6886 ,  C12Q1/6851

Abstract: The present disclosure relates to methods for providing a clinical assessment of a subject in need thereof using the gene expression level measurements from exosomes derived from the subject.

公开(公告)号：US20200032243A1

公开(公告)日：2020-01-30

申请号：US16595278

申请日：2019-10-07

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov SKOG ,  Daniel ENDERLE ,  Aparna RAMACHANDRAN ,  Haoheng YAN ,  Emily BERGHOFF ,  Tai-Fen WEI ,  Mikkel NOERHOLM

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.

公开(公告)号：US10301681B2

公开(公告)日：2019-05-28

申请号：US14909916

申请日：2014-08-06

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Mikkel Noerholm

IPC: C12Q1/6886 ,  G01N33/48

Abstract: The present invention relates generally to the field of biomarker analysis, particularly determining gene expression signatures from urine samples. The disclosure provides compositions, kits and methods for diagnosing a prostate disorder such as prostate cancer in a male subject.

公开(公告)号：US10174361B2

公开(公告)日：2019-01-08

申请号：US14357475

申请日：2012-11-12

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Leileata Russo

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6883 ,  C12N15/10 ,  C12Q1/6886

Abstract: The present invention is directed to methods of isolating particles, such as nucleic acid-containing particles or microvesicles, from a biological sample and extracting nucleic acids therefrom, wherein the biological sample is cerebrospinal fluid. The present invention further provides methods for aiding diagnosis, prognosis, monitoring and evaluation of a disease or other medical condition in a subject by detecting a biomarker associated with a disease or medical condition thereof.

公开(公告)号：US20160177401A1

公开(公告)日：2016-06-23

申请号：US14909916

申请日：2014-08-06

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Johan Karl Olov Skog ,  Mikkel NOERHOLM

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: The present invention relates generally to the field of biomarker analysis, particularly determining gene expression signatures from urine samples. The disclosure provides compositions, kits and methods for diagnosing a prostate disorder such as prostate cancer in a male subject.

Abstract translation: 本发明一般涉及生物标志物分析领域，特别是从尿液样品中确定基因表达特征。 本公开提供用于诊断男性受试者中前列腺障碍如前列腺癌的组合物，试剂盒和方法。

公开(公告)号：US20150024949A1

公开(公告)日：2015-01-22

申请号：US14508603

申请日：2014-10-07

Applicant: Exosome Diagnostics, Inc.

Inventor： Leileata M. Russo

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/158

Abstract: A method for detecting biomarkers of prostate cancer or other medical condition of the prostate based on the use of microvesicles obtained from urine samples, and the nucleic acids present in the microvesicles. The method disclosed herein are advantageous in that they may be used to support diagnosis, prognosis, monitoring, or therapy selection in lieu of or in conjunction with traditional biopsy-based diagnostics and do not require a digital rectal examination or prostate massage prior to urine sample collection.

Abstract translation: 基于从尿样获得的微泡的使用以及存在于微泡中的核酸，检测前列腺癌的生物标志物或前列腺的其它医学状况的方法。 本文公开的方法的优点在于它们可用于支持诊断，预后，监测或治疗选择，以代替传统的基于活检的诊断结合或结合传统的基于活检的诊断，并且不需要在尿液样本之前进行直肠指诊或前列腺按摩 采集。

公开(公告)号：US20250163510A1

公开(公告)日：2025-05-22

申请号：US18839547

申请日：2023-02-21

Applicant: Exosome Diagnostics, Inc. ,  The Brigham and Women's Hospital, Inc.

Inventor： Johan Karl Olov SKOG ,  Brian C. HAYNES ,  Elliot HALLMARK ,  James HURLEY ,  Jamil AZZI

IPC: C12Q1/6883 ,  G01N33/68

Abstract: The present disclosure relates to methods of identifying and treating kidney rejection in a subject comprising analyzing RNA, including microvesicular RNA.

公开(公告)号：US12018334B2

公开(公告)日：2024-06-25

申请号：US17411606

申请日：2021-08-25

Applicant: Exosome Diagnostics, Inc.

Inventor： Mikkel Noerholm ,  Susan Belzer ,  Charlotte Romain ,  Johan Karl Olov Skog ,  Leileata M. Russo ,  Wayne Comper

IPC: C12P19/34 ,  C12N7/00 ,  C12Q1/6886

CPC classification number: C12Q1/6886 ,  C12N7/00 ,  C12N2795/18122 ,  C12N2795/18131 ,  C12Q2600/106 ,  C12Q2600/118

Abstract: The present invention features a method and kit for isolating microvesicles or extracting microvesicle nucleic acids from a biological sample by using a control particle. The present invention provides control particles that are viruses or virus-like particles, such as bacteriophages, that contain control nucleic acids that can be detected to assess the accuracy, reliability, and efficiency of the microvesicle isolation or nucleic acid extraction steps. The methods described herein may further comprise the analysis of the presence, absence, or level of at least one biomarker associated with a disease or medical condition for diagnosing, prognosing, or monitoring the disease or medical condition.