公开(公告)号：US20200263139A1

公开(公告)日：2020-08-20

申请号：US16339676

申请日：2017-10-05

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Maksym A. VODYANYK ,  Xin ZHANG ,  Andrew J. BRANDL ,  Deepika RAJESH ,  Bradley SWANSON ,  Christie MUNN ,  Sarah BURTON ,  Wen Bo WANG

IPC: C12N5/074 ,  C12N5/0783 ,  C12N5/0784 ,  C12N5/0789

Abstract: Provided herein are methods for the efficient in vitro differentiation of HLA homozygous blood cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into HLA homozygous immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.

公开(公告)号：US20200172862A1

公开(公告)日：2020-06-04

申请号：US16782772

申请日：2020-02-05

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Christopher W. McMAHON ,  Lauren E. LITTLE ,  Wen Bo WANG ,  Nathaniel A. ELLIOTT

IPC: C12N5/0793 ,  A61K38/18 ,  C12N5/0797

Abstract: Methods are provided, in some aspects, for differentiating pluripotent cells into midbrain dopaminergic (DA) neurons using a mono-SMAD inhibition or inhibition of SMAD signaling with only one SMAD inhibitor. In some embodiments, mono-SMAD inhibition utilizes a single inhibitor of bone morphogenic protein (BMP) for differentiating pluripotent cells into midbrain DA neurons.

公开(公告)号：US10444227B2

公开(公告)日：2019-10-15

申请号：US14830162

申请日：2015-08-19

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Kile P. Mangan ,  Coby B. Carlson

IPC: A61K31/00 ,  G01N33/50 ,  C12N5/0793 ,  G01N33/483

Abstract: In some aspects, cultures of neurons derived from human induced pluripotent stem cells (iPS cells) that exhibit synchronous firing of neural networks are provided. In some embodiments, neuronal activity of the cultures may be detected or measured using a multi-electrode array.

公开(公告)号：US10266807B2

公开(公告)日：2019-04-23

申请号：US14244396

申请日：2014-04-03

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Deepika Rajesh ,  Sarah Alice Burton

IPC: C12N5/071 ,  C12N5/074

Abstract: Provided herein are methods for the in vitro differentiation of induced pluripotent stem cells, which have been expanded and/or maintained under defined conditions, into endodermal precursor cells (EPCs) that are capable of producing mono-hormonal beta cells.

公开(公告)号：US20180305669A1

公开(公告)日：2018-10-25

申请号：US16023685

申请日：2018-06-29

Applicant: FUJIFILM Cellular Dynamics, Inc. ,  FUJIFILM Corporation

Inventor： Michael Kenneth HANCOCK ,  Coby Bennett CARLSON ,  David A. MANN ,  Yuta MURAKAMI

IPC: C12N5/071

CPC classification number: C12N5/0671 ,  C12N2501/237 ,  C12N2506/45 ,  C12N2509/00 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

Abstract: Provided herein are methods of producing three-dimensional (3-D) microtissue from a starting cell suspension of pluripotent stem cell (PSC)-derived hepatocytes. Such a method may comprise supplementing the cell suspension with cell adhesion-promoting components and culturing the PSC-derived hepatocytes on a non-adhesive surface to produce the microtissue.

公开(公告)号：US10100282B2

公开(公告)日：2018-10-16

申请号：US13757263

申请日：2013-02-01

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Deepika Rajesh ,  Rachel Lewis

IPC: C12N5/02 ,  C12N5/0789 ,  C12N5/071

Abstract: Provided herein are methods for the in vitro maintenance, expansion, culture, and/or differentiation of pluripotent cells, such as human embryonic stem cells (hESC) or induced pluripotent cells (iPSC), into hematopoietic precursor cells or endothelial cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the pluripotent cells into hematopoietic precursor cells or endothelial cells. The resulting hematopoietic precursor cells may be further differentiated into various myeloid or lymphoid lineages.

公开(公告)号：US10093903B2

公开(公告)日：2018-10-09

申请号：US15297420

申请日：2016-10-19

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Christian Kannemeier ,  Elisabeth Enghofer ,  Lisa Harms

IPC: C12N5/00 ,  C12N5/074 ,  C12N15/85 ,  C07H21/04 ,  A01K67/00 ,  C12N5/071 ,  G01N33/50

Abstract: The present disclosure provides methods for maturing hepatocytes comprising culturing with cyclic adenosine monophosphate and a Janus kinase inhibitor. There is also provided a method for screening inhibitors of hepatitis B virus infection and/or replication.

公开(公告)号：US20240219375A1

公开(公告)日：2024-07-04

申请号：US18554103

申请日：2022-04-07

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Christopher McMAHON ,  Randall LEARISH ,  Carrie CHAVEZ ,  Cayla THOMPSON

IPC: G01N33/50 ,  C12N5/0793

CPC classification number: G01N33/5058 ,  C12N5/0619 ,  G01N33/502 ,  C12N2501/119 ,  C12N2501/13 ,  C12N2501/15 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2506/45 ,  G01N2500/10

Abstract: Midbrain dopaminergic neuronal precursor cells that can be used to treat a brain disorder are provided herein. Improved mono-SMAD methods are provided that can be used to differentiate pluripotent cells into midbrain dopaminergic (DA) neurons or midbrain neuronal precursors. In some aspects, methods are provided for mono-SMAD culture protocols and culture durations that can be used to generate dopaminergic neuronal precursor cells that have significantly improved properties for the treatment of a brain disorder such as, e.g., Parkinson's disease. Methods of treating Parkinson's disease and other brain diseases with the midbrain dopaminergic neuronal precursor cells are also provided.

公开(公告)号：US20240003871A1

公开(公告)日：2024-01-04

申请号：US18344703

申请日：2023-06-29

Applicant: FUJIFILM Cellular Dynamics, Inc. ,  FUJIFILM Holdings America Corporation

Inventor： Deepika RAJESH ,  Christie MUNN ,  Sarah BURTON ,  Ali FATHI ,  Rachel LEWIS ,  Robert BRADLEY ,  Jing LIU

IPC: G01N33/50 ,  C12N5/079

CPC classification number: G01N33/5058 ,  C12N5/0622 ,  C12N2506/45 ,  C12N2501/235 ,  C12N2500/90 ,  C12N2501/727 ,  C12N2533/90 ,  C12N2513/00 ,  C12N2501/13 ,  C12N2501/11 ,  C12N2501/25 ,  C12N2501/2301

Abstract: The present disclosure provides method for producing neural precursor cells with a glial bias from induced pluripotent cells and further differentiating the neural precursor cells to astrocytes. Further provided herein are methods for the use of the astrocytes for screening assays, models mimicking the human brain, and cell therapy.

公开(公告)号：US20220220440A1

公开(公告)日：2022-07-14

申请号：US17610123

申请日：2020-05-11

Applicant: FUJIFILM Cellular Dynamics, Inc. ,  FUJIFILM Holdings America Corporation

Inventor： Igor GUIREVICH ,  Sarah BURTON ,  Christie MUNN ,  Madelyn GOEDLAND ,  Katherine CZYSZ ,  Deepika RAJESH ,  Makiko OHSHIMA

IPC: C12N5/071 ,  G01N33/50 ,  A61K35/407 ,  A61P1/00

Abstract: The present disclosure provides methods of producing hepatocytes from induced pluripotent stem cells. Further provided herein are methods of using the hepatocytes for the treatment of a liver disease.