公开(公告)号：US20060166206A1

公开(公告)日：2006-07-27

申请号：US10533847

申请日：2003-11-17

申请人： Fyodor Urnov ,  Eric Rhodes

发明人： Fyodor Urnov ,  Eric Rhodes

IPC分类号： C12Q1/68 ,  C12M1/34 ,  B05D3/02

CPC分类号： C12Q1/6837 ,  C12N15/1034

摘要： Methods for constructing arrays of regulatory sequences, and the arrays so obtained, are provided. Regulatory sequences for use on the arrays are isolated based on their accessibility in cellular chromatin. A number of methods for using the arrays are disclosed, including regulatory DNA profiling, epigenome profiling, toxicological profiling and identification of in vivo binding sites of DNA binding proteins in complex genomes.

摘要翻译： 提供了构建调节序列阵列的方法以及如此得到的阵列。 基于其在细胞染色质中的可及性，分离用于阵列的调节序列。 公开了许多使用阵列的方法，包括调节DNA分析，表观基因组分析，毒理学分析和鉴定复合基因组中DNA结合蛋白的体内结合位点。

公开(公告)号：US06919204B2

公开(公告)日：2005-07-19

申请号：US09967869

申请日：2001-09-28

申请人： Elizabeth J. Wolffe ,  Fyodor Urnov ,  Albert Lai ,  Eva Raschke

发明人： Alan P. Wolffe

IPC分类号： C07K14/435 ,  C12N15/63 ,  C12N15/82 ,  C12N5/02

CPC分类号： C12N15/63 ,  C07K14/43581 ,  C07K2319/00 ,  C12N15/8216 ,  C12N15/822

摘要： Methods and compositions for regulating gene expression are provided. In particular, methods and compositions comprising localization domains, and fusions of localization domains with DNA binding domains and, optionally regulatory domains, are provided.

公开(公告)号：US20050032108A1

公开(公告)日：2005-02-10

申请号：US10922546

申请日：2004-08-19

申请人： Casey Case ,  Fyodor Urnov

发明人： Casey Case ,  Fyodor Urnov

IPC分类号： C12N15/09 ,  C12N15/10 ,  C12N15/12 ,  C12N15/82 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/48 ,  G06F19/00

CPC分类号： C12N15/1055 ,  C12N15/1079 ,  C12N15/8216 ,  C12N15/8241 ,  C12N15/8243 ,  C12Q1/6897 ,  G01N33/50 ,  G01N33/5005

摘要： The present disclosure provides methods and compositions for identifying transcribed regions in a genome. The methods include the use of exogenous molecules such as zinc finger proteins which are capable of binding to and modulating transcription, followed by assay for one or more selected phenotypes.

摘要翻译： 本公开提供用于鉴定基因组中转录区的方法和组合物。 所述方法包括使用能够结合和调节转录的外来分子例如锌指蛋白，随后测定一种或多种选择的表型。

公开(公告)号：US09834787B2

公开(公告)日：2017-12-05

申请号：US12798749

申请日：2010-04-08

申请人： Philip D. Gregory ,  Dirk Hockemeyer ,  Michael C. Holmes ,  Rudolf Jaenisch ,  Frank Soldner ,  Fyodor Urnov ,  Shuyuan Yao

发明人： Philip D. Gregory ,  Dirk Hockemeyer ,  Michael C. Holmes ,  Rudolf Jaenisch ,  Frank Soldner ,  Fyodor Urnov ,  Shuyuan Yao

IPC分类号： C12P21/02 ,  C12Q1/02 ,  A61K35/12 ,  C12N5/10 ,  C12N15/86 ,  C12N9/22

CPC分类号： C12N15/86 ,  C12N9/22 ,  C12N2710/10343 ,  C12N2740/15043 ,  C12N2800/30 ,  C12N2830/008

摘要： Disclosed herein are methods and compositions for targeted integration of sequences of interest such as lineage-specific or cell fate reporter constructs or protein encoding sequences into stem cells.

公开(公告)号：US20170326174A1

公开(公告)日：2017-11-16

申请号：US15640104

申请日：2017-06-30

申请人： Michael C. Holmes ,  Fyodor Urnov

发明人： Michael C. Holmes ,  Fyodor Urnov

IPC分类号： A61K35/12 ,  C07K14/715 ,  C12N15/85 ,  C12N9/22 ,  C12N9/14 ,  C07K14/435 ,  C07K14/47 ,  C12N15/62

摘要： Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.

公开(公告)号：US09506120B2

公开(公告)日：2016-11-29

申请号：US12284887

申请日：2008-09-25

申请人： Yannick Doyon ,  Fyodor Urnov

发明人： Yannick Doyon ,  Fyodor Urnov

IPC分类号： C12Q1/34 ,  C12Q1/68 ,  C12N9/22 ,  G01N33/573

CPC分类号： C12Q1/6897 ,  C12N9/22 ,  C12Q1/34 ,  G01N33/573 ,  G01N2333/922

摘要： Disclosed herein are methods and compositions for rapidly identifying and ranking nucleases for specific cleavage of a target sequence.

公开(公告)号：US08921112B2

公开(公告)日：2014-12-30

申请号：US12286668

申请日：2008-10-01

申请人： Qihua C. Cai ,  Vipula K. Shukla ,  Joseph F. Petolino ,  Lisa W. Baker ,  Robbi J. Garrison ,  Ryan C. Blue ,  Jon C. Mitchell ,  Nicole L. Arnold ,  Sarah E. Worden ,  Jeffrey Miller ,  Fyodor Urnov

发明人： Qihua C. Cai ,  Vipula K. Shukla ,  Joseph F. Petolino ,  Lisa W. Baker ,  Robbi J. Garrison ,  Ryan C. Blue ,  Jon C. Mitchell ,  Nicole L. Arnold ,  Sarah E. Worden ,  Jeffrey Miller ,  Fyodor Urnov

IPC分类号： C12N15/82 ,  C12N5/04 ,  A01H5/10 ,  A01H1/08 ,  C12N5/10

CPC分类号： C07K14/4702 ,  C07K2319/81 ,  C12N15/8213 ,  C12N15/8243

摘要： Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.

摘要翻译： 本文公开了包含CCHC锌配位残基的锌指。 还描述了锌指蛋白和包含这些CCHC锌指的融合蛋白以及编码这些蛋白的多核苷酸。 还描述了使用这些蛋白质进行基因编辑和基因调控的方法。

公开(公告)号：US08524500B2

公开(公告)日：2013-09-03

申请号：US12804234

申请日：2010-07-16

申请人： Fyodor Urnov ,  Michael C. Holmes ,  Jeffrey C. Miller ,  Carl O. Pabo

发明人： Fyodor Urnov ,  Michael C. Holmes ,  Jeffrey C. Miller ,  Carl O. Pabo

IPC分类号： C12N15/00 ,  C12N15/87

CPC分类号： A61K35/12 ,  C07K14/43595 ,  C07K14/4717 ,  C07K14/715 ,  C07K2319/00 ,  C07K2319/09 ,  C07K2319/81 ,  C12N9/14 ,  C12N9/22 ,  C12N15/62 ,  C12N15/85 ,  C12N2800/80

摘要： Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.

摘要翻译： 本文公开了用于基因组序列的靶向切割，基因组序列的靶向改变以及基因组区域和与该基因组区域同源的外源多核苷酸之间的靶向重组的方法和组合物。 组合物包括包含切割结构域（或切割半结构域）和工程化锌指结构域的融合蛋白和编码它们的多核苷酸。 用于靶向切割的方法包括将这种融合蛋白或其编码的多核苷酸引入细胞。 用于靶向重组的方法另外包括将与基因组区域同源的外源多核苷酸引入包含所公开的融合蛋白的细胞中。

公开(公告)号：US20120196370A1

公开(公告)日：2012-08-02

申请号：US13310263

申请日：2011-12-02

申请人： Fyodor URNOV ,  Jianbin Wang

发明人： Fyodor URNOV ,  Jianbin Wang

IPC分类号： C12N15/01

CPC分类号： C12N15/102 ,  C12N15/8213 ,  C12N15/907 ,  C12N2800/80

摘要： Disclosed herein are compositions and methods for generating chromosomal translocations and targeted deletions of specific lengths and at specific locations the genome of cell.

摘要翻译： 本文公开了用于产生特定长度的染色体易位和靶向缺失的组合物和方法，以及细胞基因组的特定位置。

公开(公告)号：US08071370B2

公开(公告)日：2011-12-06

申请号：US12803927

申请日：2010-07-09

申请人： Alan P. Wolffe ,  Elizabeth J. Wolffe, legal representative ,  Trevor Collingwood ,  Philip D. Gregory ,  Andrew Snowden ,  Fyodor Urnov

发明人： Alan P. Wolffe ,  Trevor Collingwood ,  Philip D. Gregory ,  Andrew Snowden ,  Fyodor Urnov

IPC分类号： C07H17/00 ,  C07K14/00

CPC分类号： C12N15/1058 ,  C07K14/4702 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/09 ,  C07K2319/24 ,  C07K2319/43 ,  C07K2319/71 ,  C07K2319/75 ,  C07K2319/81 ,  C12N15/102 ,  C12N15/1034 ,  C12N15/62 ,  C12N15/63 ,  C12N15/66 ,  C12N15/67 ,  C12Q1/6811 ,  G06F19/22 ,  G06F19/28

摘要： Methods and compositions for targeted modification of chromatin structure, within a region of interest in cellular chromatin, are provided. Such methods and compositions are useful for facilitating processes such as, for example, transcription and recombination, that require access of exogenous molecules to chromosomal DNA sequences.

摘要翻译： 提供了在细胞染色质感兴趣的区域内靶向修饰染色质结构的方法和组合物。 这些方法和组合物可用于促进需要外源分子进入染色体DNA序列的过程，例如转录和重组。