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    In situ hybridization detection method
    31.
    发明授权
    In situ hybridization detection method 有权
    原位杂交检测方法

    公开(公告)号：US09017992B2

    公开(公告)日：2015-04-28

    申请号：US11993571

    申请日：2006-06-30

    申请人： Lars Winther ,  Jesper Lohse ,  Susanne Gabs ,  Kenneth Heesche Petersen

    发明人： Lars Winther ,  Jesper Lohse ,  Susanne Gabs ,  Kenneth Heesche Petersen

    IPC分类号： C12M1/34 ,  C12Q1/68 ,  C07H21/02 ,  B01L3/00 ,  G01N31/22 ,  G01N33/52 ,  A61K47/48 ,  A61K49/00 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C08G69/02 ,  C07C231/02 ,  C07C233/36 ,  G01N33/53

    CPC分类号： C12Q1/6804 ,  A61K47/61 ,  A61K47/6843 ,  A61K47/6889 ,  A61K49/0041 ,  A61K49/0043 ,  A61K49/0056 ,  C07C231/02 ,  C07C233/36 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C08G69/02 ,  C12Q1/6876 ,  G01N33/5306

    摘要： The invention provides compositions and methods for the detection of targets in a sample; in particular, an in situ hybridization (ISH) sample. Probes and detectable labels may be provided in multiple layers in order to increase the flexibility of a detection system, and to allow for amplification to enhance the signal from a target. The layers may be created by incorporating probes and detectable labels into larger molecular units that interact through nucleic acids base-pairing, including peptide-nucleic acid (PNA) base-pairing. Optional non-natural bases allow for degenerate base pairing schemes. The compositions and methods are also compatible with immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry, enzyme immuno-assays (EIA), enzyme linked immuno-assays (ELISA), blotting methods (e.g. Western, Southern, and Northern), labeling inside electrophoresis systems or on surfaces or arrays, and precipitation, among other general detection assay formats. The invention is also compatible with many different types of targets, probes, and detectable labels.

    摘要翻译： 本发明提供用于检测样品中靶标的组合物和方法; 特别是原位杂交（ISH）样品。 可以以多层提供探针和可检测标记，以便增加检测系统的灵活性，并允许放大以增强来自靶的信号。 可以通过将探针和可检测标记掺入通过核酸碱基配对（包括肽 - 核酸（PNA）碱基配对）相互作用的较大分子单元中来产生层。 可选的非天然碱基允许简并碱基配对方案。 组合物和方法还与免疫组化（IHC），免疫细胞化学（ICC），流式细胞术，酶免疫测定（EIA），酶联免疫测定（ELISA），印迹方法（例如西方，南方和北方）相兼容， 标记电泳系统或表面或阵列，以及其他一般检测测定形式的沉淀。 本发明还与许多​​不同类型的靶，探针和可检测标记相兼容。

    Methods and compositions for immuno-histochemical detection
    33.
    发明授权
    Methods and compositions for immuno-histochemical detection 有权
    免疫组织化学检测的方法和组成

    公开(公告)号：US08632994B2

    公开(公告)日：2014-01-21

    申请号：US10999102

    申请日：2004-11-30

    申请人： Lars Winther ,  Anthony Lionel Knoll ,  Kellyann Weckworth ,  Uffe Lovborg

    发明人： Lars Winther ,  Anthony Lionel Knoll ,  Kellyann Weckworth ,  Uffe Lovborg

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54306 ,  G01N33/543 ,  G01N33/58

    摘要： The invention relates to methods and compositions of detecting a biological marker in a sample. In certain embodiments, the methods and compositions comprise a first binding agent that specifically binds to a biological marker in a sample; a second binding agent linked to a first polymer, wherein the second binding agent specifically binds to the first binding agent; a third binding agent linked to a second polymer, wherein the third binding agent specifically binds to the second binding agent; and a detectable substance. In certain embodiments the biological markers are detected immunologically.

    摘要翻译： 本发明涉及检测样品中生物标志物的方法和组合物。 在某些实施方案中，所述方法和组合物包含特异性结合样品中的生物学标记的第一结合剂; 与第一聚合物连接的第二结合剂，其中第二结合剂特异性结合第一结合剂; 与第二聚合物连接的第三粘合剂，其中所述第三粘合剂特异性结合所述第二粘合剂; 和可检测物质。 在某些实施方案中，免疫学检测生物学标记。

    Method of Pre-Treatment and Staining of a Biological Sample and Device for Support of a Biological Sample and Methods of Using Such Device
    35.
    发明申请
    Method of Pre-Treatment and Staining of a Biological Sample and Device for Support of a Biological Sample and Methods of Using Such Device 有权
    用于支持生物样品的生物样品和装置的预处理和染色的方法和使用这种装置的方法

    公开(公告)号：US20120330564A1

    公开(公告)日：2012-12-27

    申请号：US13605228

    申请日：2012-09-06

    申请人： Loren L. BLAND ,  Marc E. KEY ,  Lars WINTHER ,  David A. STANFORTH ,  Martin N. LINDBERG

    发明人： Loren L. BLAND ,  Marc E. KEY ,  Lars WINTHER ,  David A. STANFORTH ,  Martin N. LINDBERG

    IPC分类号： G06F19/00

    CPC分类号： G01N1/30 ,  B01L3/545 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0822 ,  G01N1/312 ,  G01N35/00732 ,  G01N2035/00782 ,  Y10T436/255

    摘要： There is disclosed a method of pre-treatment and staining, according to a protocol, of a biological sample disposed upon the surface of a carrier, the method comprising the step of recording at least one parameter relating to at least one protocol step in a non-volatile memory located either upon or within the carrier or a device incorporating the carrier. Also disclosed is a device comprising: a non-volatile memory; a surface of the device adapted to carry a biological sample; and communications means electrically coupled to the memory for enabling data transmission to or from an external apparatus. Also disclosed is a method of controlling processing of a biological sample disposed upon a carrier, comprising: providing, upon or within the carrier or an apparatus holding the carrier, a non-volatile memory having information relating to sample processing priority or protocol; reading the information; and scheduling the processing based upon the information.

    摘要翻译： 公开了根据方案对设置在载体表面上的生物样品进行预处理和染色的方法，所述方法包括以下步骤：将与至少一个方案步骤相关的至少一个参数记录在非 - 位于载体上或载体内的易失性存储器或包含载体的装置。 还公开了一种装置，包括：非易失性存储器; 所述装置的表面适于携带生物样品; 以及电连接到存储器的通信装置，用于使能到外部设备的数据传输。 还公开了一种控制设置在载体上的生物样品的处理的方法，包括：在载体或保持载体的装置的上方或内部提供具有与样品处理优先级或方案有关的信息的非易失性存储器; 阅读信息; 并基于该信息调度处理。

    HORIZONTAL ANTIGEN RETRIEVAL
    38.
    发明申请
    HORIZONTAL ANTIGEN RETRIEVAL 有权
    水平抗原检测

    公开(公告)号：US20100136612A1

    公开(公告)日：2010-06-03

    申请号：US12526323

    申请日：2008-02-11

    申请人： Nanna K. Christensen ,  Lars Winther

    发明人： Nanna K. Christensen ,  Lars Winther

    IPC分类号： G01N1/30

    CPC分类号： G01N33/50 ,  G01N1/30

    摘要： The present invention relates to a method for enhancing immunoreactivity of a tissue or cell sample fixed in a fixing medium, a target retrieval composition and its use. The method comprises providing a carrier in a horizontal position, said carrier having thereon a tissue or cell sample, said tissue or cell sample being on top of the carrier; contacting substantially the tissue or cell sample side of the carrier with a buffered target retrieval solution, wherein the target retrieval solution remains otherwise exposed to the environment; heating the tissue or cell sample and the target retrieval solution to a temperature above 100° C. The invention furthermore concerns the automated immunohistochemical staining of said samples, in particular the reactivation of antigens masked by fixation.

    摘要翻译： 本发明涉及一种增强固定在固定介质中的组织或细胞样品的免疫反应性的方法，目标检索组合物及其用途。 该方法包括在水平位置提供载体，所述载体上具有组织或细胞样品，所述组织或细胞样品位于载体的顶部; 基本上使载体的组织或细胞样品侧与缓冲的目标检索溶液接触，其中目标检索溶液仍然暴露于环境中; 将组织或细胞样品和目标回收溶液加热至高于100℃的温度。本发明还涉及所述样品的自动化免疫组织化学染色，特别是通过固定掩蔽的抗原的再活化。

    Removal of Embedding Medium
    39.
    发明申请
    Removal of Embedding Medium 审中-公开
    去除嵌入介质

    公开(公告)号：US20090155907A1

    公开(公告)日：2009-06-18

    申请号：US12095432

    申请日：2006-11-24

    申请人： Lars Winther ,  Martin Lindberg

    发明人： Lars Winther ,  Martin Lindberg

    IPC分类号： C12N5/06 ,  C12M1/00 ,  C12M1/36

    CPC分类号： G01N1/30 ,  G01N1/312 ,  G01N1/36 ,  G01N2001/307 ,  G01N2035/00881

    摘要： A method, apparatus and system for automated removal of an embedding medium from an embedded biological sample. The method comprising the steps of: providing an automated sample processing apparatus having an automated process operation capability that causes automated process operation events through robotic sample process functions; providing a clearing solvent, e.g. an organic solvent, capable of lowering the melting point of an embedding medium and/or dissolving an embedding medium; loading a plurality of carriers with embedded biological samples in the automated sample processing apparatus; exposing an embedded biological sample to the clearing solvent, whereby the embedding medium is liquefied; and providing a washing solution capable of removing the clearing solvent and the liquefied embedded medium from said biological sample, said clearing solvent and said washing solution being immiscible.

    摘要翻译： 一种用于从嵌入式生物样品中自动去除嵌入介质的方法，装置和系统。 该方法包括以下步骤：提供具有自动化处理操作能力的自动化样品处理设备，其通过机器人样品处理功能引起自动过程操作事件; 提供清除溶剂，例如 能够降低嵌入介质的熔点和/或溶解嵌入介质的有机溶剂; 在自动化样品处理装置中加载具有嵌入式生物样品的多个载体; 将嵌入的生物样品暴露于清除溶剂，由此嵌入介质液化; 并且提供能够从所述生物样品中除去清除溶剂和液化的嵌入介质的洗涤溶液，所述清除溶剂和所述洗涤溶液是不混溶的。

    Enhanced sample processing system and methods of biological slide processing
    40.
    发明申请
    Enhanced sample processing system and methods of biological slide processing 审中-公开
    增强样品处理系统和生物载玻片加工方法

    公开(公告)号：US20060073074A1

    公开(公告)日：2006-04-06

    申请号：US11177730

    申请日：2005-07-08

    申请人： Lars Winther

    发明人： Lars Winther

    IPC分类号： G01N35/00

    CPC分类号： G01N1/28 ,  B01F11/0266 ,  B01F13/0071 ,  B01L9/52 ,  B01L2300/0803 ,  B01L2300/0822 ,  B01L2400/0439 ,  G01N1/312 ,  G01N2035/00138 ,  G01N2035/00524

    摘要： An automated sample processing system and methods are disclosed where sample(s) are arranged on a carrier element and a process operation control system automatically processes the sample(s) perhaps robotically according to protocol and according to a scheduling system. The processing may include and be enhanced by a vibration of the sample via a vibrator disposed in or upon a sample carrier or a sample cover. Alteration of an initial aggregated event topology may be accepted while the system is processing an initial aggregation and varied-parameter robotic control simulation functionalities may be accomplished to determine an enhanced sequence for processing.

    摘要翻译： 公开了一种自动化样品处理系统和方法，其中样品被布置在载体元件上，并且过程操作控制系统可能根据协议和根据调度系统机器人自动处理样品。 处理可以包括通过设置在样品载体或样品盖中或其上的振动器的样品的振动来增强。 可以在系统正在处理初始聚合时接受初始聚合事件拓扑的改变，并且可以实现变化参数机器人控制模拟功能来确定用于处理的增强的序列。