公开(公告)号：US5416197A

公开(公告)日：1995-05-16

申请号：US136922

申请日：1993-10-15

Applicant: Jonathan A. Raper ,  Yuling Luo

Inventor： Jonathan A. Raper ,  Yuling Luo

IPC: C07K14/47 ,  C07K16/18 ,  C07K16/22

CPC classification number: C07K16/18 ,  C07K14/4703

Abstract: Essentially pure human collapsin and its uses are disclosed. An isolated nucleic acid molecule that comprises a nucleotide sequence that is complementary to a nucleotide sequence that encodes a portion of human collapsin is disclosed. Antibodies that specifically bind to human collapsin and that inhibit its activity are disclosed. Methods of inhibiting the activity of collapsin, methods of inducing neurite outgrowth and methods of treating individuals suffering from nerve damage comprising the step of contacting neuronal cells with nucleic acid molecule that comprises a nucleotide sequence that is complementary to a nucleotide sequence that encodes a portion of human collapsin or comprising the step of contacting a neuronal cell with an antibody that specifically binds to human collapsin are disclosed. Methods of identifying compounds that inhibit the activity of human collapsin comprising the steps of contacting a neuronal cell with human collapsin in the presence of a test compound are disclosed.

Abstract translation: 公开了本质上纯的人类崩解蛋白及其用途。 公开了一种分离的核酸分子，其包含与编码人collapsin的一部分的核苷酸序列互补的核苷酸序列。 公开了特异性结合人塌陷蛋白并抑制其活性的抗体。 抑制皱缩蛋白酶活性的方法，诱导神经突生长的方法以及治疗患有神经损伤的个体的方法包括使神经元细胞与核酸分子接触的步骤，该核酸分子包含与编码部分的核苷酸序列互补的核苷酸序列 公开了包含将神经元细胞与特异性结合人皱缩蛋白酶的抗体接触的步骤。 鉴定抑制人崩斑蛋白活性的化合物的方法，其包括在测试化合物存在下使神经元细胞与人凝血因子接触的步骤。

公开(公告)号：US08288522B2

公开(公告)日：2012-10-16

申请号：US13207237

申请日：2011-08-10

Applicant: Yuling Luo ,  Son Bui

Inventor： Yuling Luo ,  Son Bui

IPC: C07H21/04 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/686 ,  C12Q1/6844 ,  C12Q2537/143 ,  C12Q2561/113

Abstract: Methods for detecting and optionally quantitating one or more target nucleic acids are provided, in which a surrogate nucleic acid is captured to each target nucleic acid, amplified, and detected. Compositions, kit, and systems related to the methods are also described.

Abstract translation: 提供了用于检测和任选定量一种或多种靶核酸的方法，其中替代核酸被捕获到每个靶核酸，被扩增和检测。 还描述了与方法相关的组合物，试剂盒和系统。

公开(公告)号：US20120157348A1

公开(公告)日：2012-06-21

申请号：US12932484

申请日：2011-02-24

Applicant: Zhi Zheng ,  Yuling Luo ,  Gary McMaster

Inventor： Zhi Zheng ,  Yuling Luo ,  Gary McMaster

IPC: C40B40/06

CPC classification number: C12Q1/6816 ,  C12Q1/6837 ,  C12Q2537/143 ,  C12Q2537/125 ,  C12Q2525/313

Abstract: Methods of detecting one or more nucleic acids from whole blood or plasma are provided. The nucleic acids are captured on a solid support and detected. Compositions, kits, and systems related to the methods are also described.

Abstract translation: 提供从全血或血浆中检测一种或多种核酸的方法。 将核酸捕获在固体支持物上并检测。 还描述了与方法相关的组合物，试剂盒和系统。

公开(公告)号：US08017360B2

公开(公告)日：2011-09-13

申请号：US11595789

申请日：2006-11-09

Applicant: Yuling Luo ,  Son Bui

Inventor： Yuling Luo ,  Son Bui

IPC: C12P19/34 ,  C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/686 ,  C12Q1/6844 ,  C12Q2537/143 ,  C12Q2561/113

Abstract: Methods for detecting and optionally quantitating one or more target nucleic acids are provided, in which a surrogate nucleic acid is captured to each target nucleic acid, amplified, and detected. Compositions, kit, and systems related to the methods are also described.

Abstract translation: 提供了用于检测和任选定量一种或多种靶核酸的方法，其中替代核酸被捕获到每个靶核酸，被扩增和检测。 还描述了与方法相关的组合物，试剂盒和系统。

公开(公告)号：US07709198B2

公开(公告)日：2010-05-04

申请号：US11471025

申请日：2006-06-19

Applicant: Yuling Luo ,  Yunqing Ma ,  Cung-Tuong Nguyen

Inventor： Yuling Luo ,  Yunqing Ma ,  Cung-Tuong Nguyen

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6837 ,  C12Q1/682 ,  C12Q1/6841 ,  C12Q2537/143 ,  C12Q2537/125 ,  C12Q2565/543 ,  C12Q2525/313

Abstract: Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.

Abstract translation: 提供了检测核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测到在固体支持物上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。 还描述了与方法相关的组合物，试剂盒和系统。

公开(公告)号：US20080050746A1

公开(公告)日：2008-02-28

申请号：US11893356

申请日：2007-08-14

Applicant: Gary McMaster ,  Joan Davies ,  Yunqing Ma ,  Yuling Luo

Inventor： Gary McMaster ,  Joan Davies ,  Yunqing Ma ,  Yuling Luo

IPC: C12N5/02 ,  C12Q1/68

CPC classification number: C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  C12Q1/6841 ,  Y10T436/143333 ,  C12Q2527/101 ,  C12Q2523/32 ,  C12Q2521/537 ,  C12Q2545/101

Abstract: This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solublize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.

Abstract translation: 本发明提供了定量来自有问题样品的核酸的方法，例如老化样品，福尔马林固定样品，石蜡包埋样品，具有非整倍体细胞的样品和具有片段化核酸的细胞。 方法包括在非变性条件下有效地从保存的临床样品中纯化核酸而不用有机提取的技术，使细胞计数正常化而不管非整倍体，以获得核酸的断裂状态，并为降解的核酸提供标准曲线 酸样。

公开(公告)号：US06583277B2

公开(公告)日：2003-06-24

申请号：US09771467

申请日：2001-01-26

Applicant: Yuling Luo ,  Xiaomei Xu

Inventor： Yuling Luo ,  Xiaomei Xu

IPC: C07H2104

CPC classification number: C07K14/4703 ,  A61K38/00

Abstract: The invention provides methods and compositions relating to semaphorin K1 (sema K1) polypeptides which regulate cellular guidance and physiology, and related nucleic acids. The polypeptides may be produced recombinantly from transformed host cells from the disclosed sema K1 encoding nucleic acids or purified from human cells. The invention provides isolated sema K1 hybridization probes and primers capable of specifically hybridizing with the disclosed sema K1 genes, sema K1-specific binding agents such as specific antibodies, and methods of making and using the subject compositions in diagnosis, therapy and in the biopharmaceutical industry.

公开(公告)号：US06511824B1

公开(公告)日：2003-01-28

申请号：US09362842

申请日：1999-07-28

Applicant: Andrew Roy Buchman ,  Christian Burks ,  Helen Louise Francis-Lang ,  Lucile A. Gillett ,  Jonathan Cagampang Heller ,  Casey Casimir Kopczynski ,  Jonathan Scott Margolis ,  Darren Mark Platt ,  Bindu Priya Reddy ,  Candace Swimmer ,  John W. Winslow ,  Yuling Luo

Inventor： Andrew Roy Buchman ,  Christian Burks ,  Helen Louise Francis-Lang ,  Lucile A. Gillett ,  Jonathan Cagampang Heller ,  Casey Casimir Kopczynski ,  Jonathan Scott Margolis ,  Darren Mark Platt ,  Bindu Priya Reddy ,  Candace Swimmer ,  John W. Winslow ,  Yuling Luo

IPC: C12N1500

CPC classification number: C07K14/43581 ,  A01K67/0333 ,  A01K67/0336 ,  A01K67/0337 ,  A01K67/0339 ,  A01K2217/05 ,  A01K2217/075 ,  C07K14/705 ,  C07K14/70571

Abstract: Tandem pore domain weak inward rectifying K+ (TWIK) channel nucleic acids and proteins that have been isolated from Drosophila melanogaster and Leptinotarsa are described. The TWIK channel nucleic acids and proteins can be used to genetically modify metazoan invertebrate organisms, such as insects, coelomates, and pseudocoelomates, or cultured cells, resulting in TWIK channel expression or mis-expression. The genetically modified organisms or cells can be used in screening assays to identify candidate compounds which are potential pesticidal agents or therapeutics that interact with TWIK channel proteins. They can also be used in methods for studying TWIK channel activity and identifying other genes that modulate the function of, or interact with, the TWIK channel gene.

Abstract translation: 描述了从黑腹果蝇和Leptinotarsa分离的串联孔结构域弱内向整流K +（TWIK）通道核酸和蛋白质。 TWIK通道核酸和蛋白质可用于遗传修饰后生动物无脊椎动物生物体，如昆虫，群落和假细菌，或培养细胞，导致TWIK通道表达或错误表达。 遗传修饰的生物体或细胞可用于筛选测定以鉴定候选化合物，其是潜在的杀虫剂或与TWIK通道蛋白相互作用的治疗剂。 它们也可用于研究TWIK通道活性的方法，并鉴定调节TWIK通道基因功能或与之相互作用的其他基因。

公开(公告)号：US06225285B1

公开(公告)日：2001-05-01

申请号：US09041236

申请日：1998-03-11

Applicant: Yuling Luo ,  Xiaomei Xu

Inventor： Yuling Luo ,  Xiaomei Xu

IPC: A61K3800

CPC classification number: C07K14/4703 ,  A61K38/00

Abstract: The invention provides methods and compositions relating to semaphorin K1 (sema K1) polypeptides which regulate cellular guidance and physiology, and related nucleic acids. The polypeptides may be produced recombinantly from transformed host cells from the disclosed sema K1 encoding nucleic acids or purified from human cells. The invention provides isolated sema K1 hybridization probes and primers capable of specifically hybridizing with the disclosed sema K1 genes, sema K1-specific binding agents such as specific antibodies, and methods of making and using the subject compositions in diagnosis, therapy and in the biopharmaceutical industry.

Abstract translation: 本发明提供了与调节细胞指导和生理学的信号素K1（sema K1）多肽以及相关核酸相关的方法和组合物。 可以从所公开的编码核酸的半胱氨酸K1的转化的宿主细胞重组产生多肽或从人细胞中纯化多肽。 本发明提供了能够与所公开的半碱基K1基因，半胱氨酸K1特异性结合剂如特异性抗体特异性杂交的分离的半碱基K1杂交探针和引物，以及在诊断，治疗和生物制药行业中制备和使用受试者组合物的方法 。