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公开(公告)号：US5989169A
公开(公告)日：1999-11-23
申请号：US600908
申请日：1996-02-13
IPC分类号： C12N15/09 , C11D3/386 , C12N1/21 , C12N9/28 , C12N15/00 , C12N15/56 , C12R1/07 , C12N9/26 , C07H21/04
CPC分类号： C12N9/2417 , C11D3/386
摘要： The present invention relates to a method of constructing a variant of a parent Termamyl-like .alpha.-amylase, which variant has .alpha.-amylase activity and at least one altered property as compared to the parent .alpha.-amylase, comprising i) analyzing the structure of the parent Termamyl-like .alpha.-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like .alpha.-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like .alpha.-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like .alpha.-amylase variant, which as compared to the parent Termamyl-like .alpha.-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and iii) testing the resulting Termamyl-like .alpha.-amylase variant for the property in question.
摘要翻译： 本发明涉及一种构建母体类戊酰样α-淀粉酶的变体的方法，该变体与亲本α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，其包括：i）分析 鉴定至少一个氨基酸残基或类甲酰样α-淀粉酶结构的至少一个结构部分，所述氨基酸残基或结构部分被认为与改变其性质有关 母体类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比的类戊酰样α-淀粉酶变体，已经在 在i）中鉴定的氨基酸残基或结构部分，以便改变其性质，和iii）测试所得到的类戊酰样α-淀粉酶变体。
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公开(公告)号：US08367390B2
公开(公告)日：2013-02-05
申请号：US12420446
申请日：2009-04-08
申请人： Leonardo De Maria , Allan Svendsen , Torben Vedel Borchert , Lars Lehmann Hylling Christensen , Sine Larsen , Carsten Ryttersgaard
发明人： Leonardo De Maria , Allan Svendsen , Torben Vedel Borchert , Lars Lehmann Hylling Christensen , Sine Larsen , Carsten Ryttersgaard
CPC分类号： C12N9/2468 , C12Y302/01089
摘要： The present invention relates to variants of Glycoside Hydrolase family 53 galactanases, e.g., variants of the galactanases from strains of Yersinia, Aspergillus, Humicola, Meripilus, Myceliophthora, Thermomyces, Bacillus, Bifidobacterium, Cellvibrio, Clostridium, Pseudomonas, Thermotoga, or Xanthomonas.
摘要翻译： 本发明涉及糖苷水解酶家族53半乳聚糖酶的变体，例如来自耶尔森氏菌，曲霉属，腐质霉属，Meripilus，Myceliophthora，Thermomyces，Bacillus，Bifidobacterium，Cellvibrio，Clostridium，Pseudomonas，Thermotoga或黄单胞菌属的半乳聚糖酶的变体。
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公开(公告)号：US20120282672A1
公开(公告)日：2012-11-08
申请号：US13557313
申请日：2012-07-25
CPC分类号： C12N9/2417 , C11D3/386 , C12P19/02 , C12P19/14
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.
摘要翻译： 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶在下列特性中表现出至少一个变化：底物特异性，底物结合，底物 裂解模式，热稳定性，pH /活性分布，pH /稳定性分布，对氧化的稳定性，Ca 2+依赖性和比活性。
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公开(公告)号：US20100144575A1
公开(公告)日：2010-06-10
申请号：US12100506
申请日：2008-04-10
IPC分类号： C12N9/28 , G01N33/53 , C12Q1/40 , C12N9/30 , C12N1/21 , C12N1/15 , C12N15/75 , C12N15/80 , C11D3/386 , C12N15/56
CPC分类号： C11D3/386 , C12N9/2417
摘要： The present invention relates to a method of constructing a variant of a parent Termamyl-like α-amylase, which variant has α-amylase activity and at least one altered property as compared to the parent α-amylase, comprises i) analysing the structure of the parent Termamyl-like α-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like α-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like α-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like α-amylase variant, which as compared to the parent Termamyl-like α-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like α-amylase variant with respect to the property in question.
摘要翻译： 本发明涉及构建母体类戊酰样α-淀粉酶的变体的方法，该变体与母体α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，包括：i）分析 鉴定至少一个氨基酸残基或类戊酰样α-淀粉酶结构的至少一个结构部分，该氨基酸残基或结构部分被认为与改变 母体类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比的类戊酰样α-淀粉酶变体，已经在 在i）中鉴定的氨基酸残基或结构部分，以改变性质，以及任选地，iii）相对于所讨论的性质测试所得到的类戊酰样α-淀粉酶变体。
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公开(公告)号：US20090317864A1
公开(公告)日：2009-12-24
申请号：US12493860
申请日：2009-06-29
申请人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard
发明人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38609 , C11D3/38618 , C12Y302/01001
摘要： The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).
摘要翻译： 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度（相对于亲本）下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体在酸性pH和/或低Ca 2+浓度（相对于亲本）中表现出增加的热稳定性。
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公开(公告)号：US06867031B2
公开(公告)日：2005-03-15
申请号：US10025648
申请日：2001-12-19
CPC分类号： C11D3/38618 , C11D3/38609 , C11D3/38681 , C12N9/2417 , C12Y302/01001 , D06L1/14
摘要： The present invention relates to variants of a parent α-amylase, which parent α-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an α-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an α-amylase having one of these amino acid sequences; in which variant: (a) at least one amino acid residue of the parent α-amylase has been deleted; and/or (b) at least one amino acid residue of the parent α-amylase has been replaced by a different amino acid residue; and/or (c) at least one amino acid residue has been inserted relative to the parent α-amylase; the variant having α-amylase activity and exhibiting at least one of the following properties relative to the parent α-amylase: increased thermostability; increased stability towards oxidation; and reduced Ca2+ dependency; with the proviso that the amino acid sequence of the variant is not identical to any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 7, respectively.
摘要翻译： 本发明涉及母体α-淀粉酶的变体，其母体α-淀粉酶（i）具有选自SEQ ID No.1，SEQ ID No.2，SEQ ID No.2所示的氨基酸序列的氨基酸序列。 3和SEQ ID No.7; 或（ii）与这些氨基酸序列中的一个或多个显示至少80％的同源性; 和/或显示与针对具有这些氨基酸序列之一的α-淀粉酶产生的抗体的免疫交叉反应性; 和/或由与编码具有这些氨基酸序列之一的α-淀粉酶的DNA序列相同的探针杂交的DNA序列编码; 在该变体中：（a）母体α-淀粉酶的至少一个氨基酸残基已被缺失; 和/或（b）母体α-淀粉酶的至少一个氨基酸残基被不同的氨基酸残基替代; 和/或（c）相对于母体α-淀粉酶插入至少一个氨基酸残基; 该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶表现出以下特性中的至少一个：增加的热稳定性; 提高氧化稳定性; 并降低Ca 2+依赖性;条件是变体的氨基酸序列与SEQ ID No.1，SEQ ID No.2，SEQ ID No.3和SEQ ID No.3所示的任何氨基酸序列不同。 SEQ ID No.7。
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公开(公告)号：US06204232B1
公开(公告)日：2001-03-20
申请号：US09183412
申请日：1998-10-30
申请人： Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Søren Kjærulff
发明人： Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Søren Kjærulff
IPC分类号： C12N928
CPC分类号： C12N9/2417 , C07K14/32 , C11D3/386 , C11D3/38609 , C11D3/38636 , C12Y302/01001
摘要： The invention relates to a variant of a parent Termamyl-like &agr;-amylase, which exhibits an alteration in at least one of the following properties relative to said parent &agr;-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.
摘要翻译： 本发明涉及亲本的类戊酰样α-淀粉酶的变体，其相对于所述亲本α-淀粉酶表现出至少一种以下特性的改变：i）在8至10.5的pH下改善的pH稳定性; 和/或ii）在pH8至10.5时提高的Ca 2+稳定性，和/或iii）在10至60℃的温度下提高比活性。
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公开(公告)号：US5753460A
公开(公告)日：1998-05-19
申请号：US720899
申请日：1996-10-10
申请人： Henrik Bisgard-Frantzen , Torben Vedel Borchert , Allan Svendsen , Marianne Thellersen , Pia Van der Zee
发明人： Henrik Bisgard-Frantzen , Torben Vedel Borchert , Allan Svendsen , Marianne Thellersen , Pia Van der Zee
IPC分类号： C12N15/09 , C11D3/386 , C12N9/28 , C12N15/56 , C12R1/125 , D06L1/14 , C12P21/06 , C07H21/04 , C12N1/20 , C12N15/00
CPC分类号： C12N9/2417 , C11D3/386 , D06L1/14
摘要： A variant of a parent .alpha.-amylase enzyme having an improved washing and/or dishwashing performance as compared to the parent enzyme, wherein one or more amino acid residues of the parent enzyme have been replaced by a different amino acid residue and/or wherein one or more amino acid residues of the parent .alpha.-amylase have been deleted and/or wherein one or more amino acid residues have been added to the parent .alpha.-amylase enzyme, provided that the variant is different from one in which the methionine residue in position 197 of a parent B. licheniformis .alpha.-amylase has been replaced by alanine or threonine, as the only modification being made. The variant may be used for washing and dishwashing.
摘要翻译： 与母体酶相比，具有改善的洗涤和/或餐具洗涤性能的母体α-淀粉酶的变体，其中母体酶的一个或多个氨基酸残基已被不同的氨基酸残基替代和/或其中一个 或更多的母体α-淀粉酶的氨基酸残基已经缺失和/或其中一个或多个氨基酸残基已加入到母体α-淀粉酶中，条件是该变体不同于其中位置上的甲硫氨酸残基的变体 作为唯一的修改，母本地衣芽孢杆菌α-淀粉酶的197已被丙氨酸或苏氨酸代替。 该变体可用于洗涤和洗碗。
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公开(公告)号：US06291165B1
公开(公告)日：2001-09-18
申请号：US09040025
申请日：1998-03-17
IPC分类号： C12Q168
CPC分类号： C12N15/1027
摘要： The present invention relates to a new method of shuffling especially heterologous polynucleotide sequences, screening and/or selection of new recombinant proteins resulting therefrom having a desired biological activity, and especially to production and identification of novel proteases exhibiting desired properties.
摘要翻译： 本发明涉及一种混合洗涤方法，特别是异源多核苷酸序列，筛选和/或选择由此产生的新的重组蛋白质具有所需的生物学活性，特别是涉及产生和鉴定出具有所需性质的新型蛋白酶。
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公开(公告)号：US08288144B2
公开(公告)日：2012-10-16
申请号：US12481922
申请日：2009-06-10
申请人： Sanne Schroder Glad , Carsten Andersen , Torben Vedel Borchert , Katja Salomon Johansen , Henrik Frisner , Mads Eskelund Bjornvad , Thomas Thisted
发明人： Sanne Schroder Glad , Carsten Andersen , Torben Vedel Borchert , Katja Salomon Johansen , Henrik Frisner , Mads Eskelund Bjornvad , Thomas Thisted
CPC分类号： C12N9/88 , C11D3/38636
摘要： The present invention relates to pectate lyase variants exhibiting alterations relative to a parent enzyme exhibiting pectate lyase activity as its major enzymatic activity; to a method of producing such enzymes; and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries. Compared to the parent enzyme, the pectate lyase variants of the present invention exhibit improved stability in detergents.
摘要翻译： 本发明涉及相对于显示出果胶裂解酶活性的亲本酶作为其主要酶活性的改变的果胶酸裂解酶变体; 涉及一种生产这种酶的方法; 以及在纺织，洗涤剂和纤维素纤维加工业中使用这些酶的方法。 与亲本酶相比，本发明的果胶酸裂解酶变体表现出改进的洗涤剂稳定性。
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