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31.发明申请公开(公告)号：US20110098207A1
公开(公告)日：2011-04-28
申请号：US12443241
申请日：2007-09-26
申请人： Marguerite A. Cervin
发明人： Marguerite A. Cervin
摘要： The present invention provides recombinant bacterial cells for producing a detergent-additive protein. In some embodiments, the cells are of the genus Bacillus. In additional embodiments, the cells comprise a genome comprising an inactivated bglC gene, as well as a recombinant nucleic acid for production of at least one secreted detergent-additive protein. In some preferred embodiments, the secreted detergent-additive protein is a protease. The present invention also provides methods of using the bacterial cells to produce at least one detergent-additive protein, as well as cellulase-free compositions containing at least one detergent-additive protein.
摘要翻译： 本发明提供了用于生产洗涤剂添加剂蛋白质的重组细菌细胞。 在一些实施方案中，细胞是芽孢杆菌属。 在另外的实施方案中，细胞包含包含失活的bglC基因的基因组，以及用于产生至少一种分泌的洗涤剂 - 添加剂蛋白质的重组核酸。 在一些优选的实施方案中，分泌的洗涤剂添加剂蛋白质是蛋白酶。 本发明还提供使用细菌细胞产生至少一种洗涤剂 - 添加剂蛋白质的方法，以及含有至少一种洗涤剂 - 添加剂蛋白质的不含纤维素酶的组合物的方法。
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32.发明申请公开(公告)号：US20090098249A1
公开(公告)日：2009-04-16
申请号：US12027127
申请日：2008-02-06
申请人： Marguerite A. Cervin , Oliver Kensch , Ulrich Kettling , Steven S. Kim , Birgitta Leuthner , Andrei Miasnikov , Michael Ward , Klaus Pellengahr
发明人： Marguerite A. Cervin , Oliver Kensch , Ulrich Kettling , Steven S. Kim , Birgitta Leuthner , Andrei Miasnikov , Michael Ward , Klaus Pellengahr
IPC分类号： A23K1/165 , C12N9/14 , C12N15/11 , C12N15/00 , C12N1/19 , C12N9/24 , C12N9/50 , C12N9/42 , C12P21/04
摘要： The present invention relates to variant phytase enzymes having altered properties.
摘要翻译： 本发明涉及具有改变性质的变体植酸酶。
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33.发明授权公开(公告)号：US07371558B2
公开(公告)日：2008-05-13
申请号：US10680286
申请日：2003-10-06
摘要： The present invention provides a microorganism useful for biologically producing 1,3-propanediol from a fermentable carbon source at higher yield than was previously known. The complexity of the cofactor requirements necessitates the use of a whole cell catalyst for an industrial process that utilizes this reaction sequence to produce 1,3-propanediol. The invention provides a microorganism with disruptions in specified genes and alterations in the expression levels of specified genes that is useful in a higher yielding process to produce 1,3-propanediol.
摘要翻译： 本发明提供了一种可用于从可发酵碳源生产1,3-丙二醇的微生物，其产率高于之前已知的。 辅助因子要求的复杂性需要使用全细胞催化剂用于工业过程，其利用该反应顺序产生1,3-丙二醇。 本发明提供了一种在特定基因中具有破坏的微生物，并且在用于产生1,3-丙二醇的更高产率的方法中有用的特定基因的表达水平的改变。
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公开(公告)号：US08512982B2
公开(公告)日：2013-08-20
申请号：US12743187
申请日：2008-12-19
摘要： The present invention relates to cells that have been genetically manipulated to have an altered capacity to express and/or produce proteins of interest. In particular, the present invention relates to modified host cells of Gram-positive microorganisms, such as Bacillus species that are capable of overexpressing ymaH. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode YmaH, and the modified host cells comprising them. In particular, the present invention relates to compositions and methods of overexpressing YmaH for enhancing the expression and production of proteins of interest (e.g., proteases) in Bacillus species.
摘要翻译： 本发明涉及已被遗传操作以具有改变的表达和/或产生感兴趣的蛋白质的能力的细胞。 特别地，本发明涉及革兰氏阳性微生物的修饰宿主细胞，例如能够过表达ymaH的芽孢杆菌属物种。 本发明包括含有编码YmaH的多核苷酸序列的多核苷酸构建体和表达载体，以及包含它们的修饰的宿主细胞。 特别地，本发明涉及过表达YmaH用于增强芽孢杆菌属物种中目的蛋白质（例如蛋白酶）的表达和产生的组合物和方法。
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35.发明授权公开(公告)号：US08383567B2
公开(公告)日：2013-02-26
申请号：US12443241
申请日：2007-09-26
申请人： Marguerite A. Cervin
发明人： Marguerite A. Cervin
摘要： The present invention provides recombinant bacterial cells for producing a detergent-additive protein. In some embodiments, the cells are of the genus Bacillus. In additional embodiments, the cells comprise a genome comprising an inactivated bglC gene, as well as a recombinant nucleic acid for production of at least one secreted detergent-additive protein. In some preferred embodiments, the secreted detergent-additive protein is a protease. The present invention also provides methods of using the bacterial cells to produce at least one detergent-additive protein, as well as cellulase-free compositions containing at least one detergent-additive protein.
摘要翻译： 本发明提供了用于生产洗涤剂添加剂蛋白质的重组细菌细胞。 在一些实施方案中，细胞是芽孢杆菌属。 在另外的实施方案中，细胞包含包含失活的bglC基因的基因组，以及用于产生至少一种分泌的洗涤剂 - 添加剂蛋白质的重组核酸。 在一些优选的实施方案中，分泌的洗涤剂添加剂蛋白质是蛋白酶。 本发明还提供使用细菌细胞产生至少一种洗涤剂 - 添加剂蛋白质的方法，以及含有至少一种洗涤剂 - 添加剂蛋白质的不含纤维素酶的组合物的方法。
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36.发明申请Method of Creating a Library of Bacterial Clones with Varying Levels of Gene Expression 有权创建不同基因表达水平的细菌克隆库的方法公开(公告)号：US20120015849A1
公开(公告)日：2012-01-19
申请号：US13155290
申请日：2011-06-07
CPC分类号： C12N15/1086 , C40B40/08 , C40B50/06
摘要： The present invention relates to a method of creating DNA libraries that include an artificial promoter library and/or a modified ribosome binding site library and transforming bacterial host cells with the library to obtain a population of bacterial clones having a range of expression levels for a chromosomal gene of interest.
摘要翻译： 本发明涉及一种创建包含人源启动子文库和/或修饰的核糖体结合位点文库的DNA文库的方法，并用文库转化细菌宿主细胞，以获得具有染色体表达水平范围的细菌克隆群 感兴趣的基因
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公开(公告)号：US20110117598A1
公开(公告)日：2011-05-19
申请号：US12743187
申请日：2008-12-19
摘要： The present invention relates to cells that have been genetically manipulated to have an altered capacity to express and/or produce proteins of interest. In particular, the present invention relates to modified host cells of Gram-positive microorganisms, such as Bacillus species that are capable of overexpressing ymaH. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode YmaH, and the modified host cells comprising them. In particular, the present invention relates to compositions and methods of overexpressing YmaH for enhancing the expression and production of proteins of interest (e.g., proteases) in Bacillus species.
摘要翻译： 本发明涉及已被遗传操作以具有改变的表达和/或产生感兴趣的蛋白质的能力的细胞。 特别地，本发明涉及革兰氏阳性微生物的修饰宿主细胞，例如能够过表达ymaH的芽孢杆菌属物种。 本发明包括含有编码YmaH的多核苷酸序列的多核苷酸构建体和表达载体，以及包含它们的修饰的宿主细胞。 特别地，本发明涉及过表达YmaH用于增强芽孢杆菌属物种中目的蛋白质（例如蛋白酶）的表达和产生的组合物和方法。
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公开(公告)号：US20090311395A1
公开(公告)日：2009-12-17
申请号：US12085721
申请日：2006-12-08
申请人： Marguerite A. Cervin , Gregg Whited
发明人： Marguerite A. Cervin , Gregg Whited
CPC分类号： C12P7/00 , A61K31/327 , A62D3/02 , A62D3/38 , A62D2101/02 , A62D2203/04 , C12N9/1025
摘要： The present invention provides an enzyme system that efficiently generates peracetic acid for use in decontamination applications. In preferred embodiments, the present invention provides a system that comprises an ester substrate, a hydrogen peroxide, and at least one acyl transferase. In some particularly preferred embodiments, the system further comprises at least one surfactant. In alternatively preferred embodiments, the present invention provides at least one wild-type and/or variant acyl transferase. The present invention finds particular use in decontamination involving a wide variety of chemical and biological warfare materials, as well as for general surface cleaning and decontamination.
摘要翻译： 本发明提供了一种有效地生产用于去污应用的过乙酸的酶系统。 在优选的实施方案中，本发明提供了一种包含酯底物，过氧化氢和至少一种酰基转移酶的体系。 在一些特别优选的实施方案中，系统还包含至少一种表面活性剂。 在另外优选的实施方案中，本发明提供至少一种野生型和/或变体酰基转移酶。 本发明特别用于涉及各种化学和生物战争材料的净化，以及一般的表面清洁和去污。
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39.发明申请公开(公告)号：US20080220498A1
公开(公告)日：2008-09-11
申请号：US11714487
申请日：2007-03-06
申请人： Marguerite A. Cervin , Oliver Kensch , Ulrich Kettling , Birgitta Leuthner , Andrei Miasnikov , Klaus Pellengahr
发明人： Marguerite A. Cervin , Oliver Kensch , Ulrich Kettling , Birgitta Leuthner , Andrei Miasnikov , Klaus Pellengahr
CPC分类号： C12N9/16 , A23K20/189 , A23K50/10 , A23K50/30 , A23K50/75 , A23K50/80 , C12Y301/03008 , C12Y301/03026 , C13K1/06 , Y02A40/818 , Y02E50/17
摘要： The present invention relates to variant phytase enzymes having altered properties.
摘要翻译： 本发明涉及具有改变性质的变体植酸酶。
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