公开(公告)号：US20200010834A1

公开(公告)日：2020-01-09

申请号：US16460025

申请日：2019-07-02

Applicant: Codexis, Inc.

Inventor： Scott J. Novick ,  Nikki Dellas ,  Vesna Mitchell ,  Da Duan ,  Jovana Nazor ,  Oscar Alvizo ,  Auric Anthony Sowell-Kantz ,  Jeffrey C. Moore ,  Mark Huffman ,  Agustina Rodriguez-Granillo ,  Deeptak Verma ,  Nicholas M. Marshall ,  Jay Russell ,  Keith A. Canada

IPC: C12N15/63

Abstract: The present invention provides engineered purine nucleoside phosphorylase (PNP) enzymes, polypeptides having PNP activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PNP enzymes are also provided. The present invention further provides compositions comprising the PNP enzymes and methods of using the engineered PNP enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

公开(公告)号：US20240409916A1

公开(公告)日：2024-12-12

申请号：US18813482

申请日：2024-08-23

Applicant: Codexis, Inc.

Inventor： Oscar Alvizo ,  David Elgart ,  Robert Kevin Orr ,  James Nicholas Riggins ,  Anna Fryszkowska ,  Katrina W. Lexa ,  Xiang Yi ,  Da Duan ,  Courtney Dianne Moffett ,  Nikki Dellas ,  Vesna Mitchell

IPC: C12N9/84 ,  C07K14/62 ,  C12P21/02

Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, and methods of using the engineered PGA enzymes.

公开(公告)号：US20240409912A1

公开(公告)日：2024-12-12

申请号：US18679281

申请日：2024-05-30

Applicant: Codexis, Inc. ,  Société des Produits Nestlé S.A.

Inventor： Chinping Chng ,  Ruth L. Cong ,  Da Duan ,  Brian Ferrer ,  Ravi David Garcia ,  Nikki D. Kruse ,  Hirdesh Kumar ,  Stephen Joshua Macaso Millet ,  Trica Windgassen ,  Liang Zhu

IPC: C12N9/54 ,  C12N9/96

Abstract: The present disclosure provides engineered protease polypeptides, recombinant polynucleotides encoding the engineered protease polypeptides, and uses of the engineered protease polypeptides in therapeutic applications.

公开(公告)号：US20240068005A1

公开(公告)日：2024-02-29

申请号：US18352899

申请日：2023-07-14

Applicant: Codexis, Inc.

Inventor： Jeffrey C. Moore ,  Jack Liang ,  Jonathan Penfield ,  Jovana Nazor ,  Nikki Dellas ,  Vesna Mitchell ,  Da Duan ,  Iman Farasat ,  Agustina Rodriguez-Granillo ,  Grant Murphy ,  Nicholas Marshall

IPC: C12P41/00 ,  C12N9/02 ,  C12N9/04 ,  C12N15/70

CPC classification number: C12P41/002 ,  C12N9/0004 ,  C12N9/0006 ,  C12N15/70 ,  C12Y101/01002 ,  C12Y120/01001

Abstract: The present invention provides engineered ketoreductase and phosphite dehydrogenase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase and phosphite dehydrogenase enzymes, as well as polynucleotides encoding the engineered ketoreductase and phosphite dehydrogenase enzymes, host cells capable of expressing the engineered ketoreductase and phosphite dehydrogenase enzymes, and methods of using the engineered ketoreductase and phosphite dehydrogenase enzymes to synthesize a chiral catalyst used in the synthesis of antiviral compounds, such as nucleoside inhibitors. The present invention further provides methods of using the engineered enzymes to deracemize a chiral alcohol in a one-pot, multi-enzyme system.

公开(公告)号：US11913037B2

公开(公告)日：2024-02-27

申请号：US17711382

申请日：2022-04-01

Applicant: Codexis, Inc.

Inventor： Da Duan ,  Auric Anthony Sowell-Kantz ,  Aksiniya Lyubenova Petkova ,  Oscar Alvizo

IPC: C12N9/12 ,  C12N1/21 ,  C12N15/52 ,  C12P21/02

CPC classification number: C12N9/1229 ,  C12Y207/04003

Abstract: The present invention provides engineered adenylate kinase (AdK) enzymes, polypeptides having AdK activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing AdK enzymes are also provided. The present invention further provides compositions comprising the AdK enzymes and methods of using the engineered AdK enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

公开(公告)号：US20240060064A1

公开(公告)日：2024-02-22

申请号：US18467259

申请日：2023-09-14

Applicant: Codexis, Inc.

Inventor： Scott J. Novick ,  Xiang Yi ,  Nikki Dellas ,  Oscar Alvizo ,  Jovana Nazor ,  Da Duan ,  Vesna Mitchell ,  Jonathan Vroom ,  Santhosh Sivaramakrishnan ,  Nandhitha Subramanian ,  Jeffrey C. Moore ,  Mark Huffman ,  Agustina Rodriguez-Granillo ,  Deeptak Verma ,  Grant S. Murphy ,  Nicholas Marshall ,  Jay Russell ,  Keith A. Canada

IPC: C12N9/90 ,  C12N15/52 ,  C12N15/74 ,  C12N15/81

CPC classification number: C12N9/90 ,  C12N15/52 ,  C12N15/74 ,  C12N15/815 ,  C12Y504/02007

Abstract: The present invention provides engineered phosphopentomutase (PPM) enzymes, polypeptides having PPM activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PPM enzymes are also provided. The present invention further provides compositions comprising the PPM enzymes and methods of using the engineered PPM enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

公开(公告)号：US20240026326A1

公开(公告)日：2024-01-25

申请号：US18462237

申请日：2023-09-06

Applicant: Codexis, Inc. ,  Societe des Produits Nestle S.A.

Inventor： Chinping Chng ,  Nikki Dellas ,  Da Duan ,  Ravi David Garcia ,  Harvinder Chagger Maniar

IPC: C12N9/26 ,  A61P1/18 ,  A61K38/47

CPC classification number: C12N9/2414 ,  A61P1/18 ,  A61K38/47 ,  C12Y302/01001

Abstract: The present invention provides engineered amylase polypeptides and compositions thereof. The engineered amylase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH

公开(公告)号：US20220325284A1

公开(公告)日：2022-10-13

申请号：US17711401

申请日：2022-04-01

Applicant: Codexis, Inc.

Inventor： Da Duan ,  Auric Anthony Sowell-Kantz ,  Aksiniya Lyubenova Petkova ,  Oscar Alvizo

IPC: C12N15/52 ,  C12N9/12 ,  C12N15/63

Abstract: The present invention provides engineered guanylate kinase (GK) enzymes, polypeptides having GK activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing GK enzymes are also provided. The present invention further provides compositions comprising the GK enzymes and methods of using the engineered GK enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

公开(公告)号：US20220325257A1

公开(公告)日：2022-10-13

申请号：US17711382

申请日：2022-04-01

Applicant: Codexis, Inc.

Inventor： Da Duan ,  Auric Anthony Sowell-Kantz ,  Aksiniya Lyubenova Petkova ,  Oscar Alvizo

IPC: C12N9/12

Abstract: The present invention provides engineered adenylate kinase (AdK) enzymes, polypeptides having AdK activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing AdK enzymes are also provided. The present invention further provides compositions comprising the AdK enzymes and methods of using the engineered AdK enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

公开(公告)号：US20220090033A1

公开(公告)日：2022-03-24

申请号：US17420378

申请日：2020-01-13

Applicant: Codexis, Inc.

Inventor： Jovana Nazor ,  Christopher Michael Micklitsch ,  Da Duan ,  Auric Anthony Sowell-Kantz ,  Steven Miller ,  Birgit Kosjek ,  Inman Farasat ,  Nicholas M. Marshall

IPC: C12N9/10 ,  C12P13/00

Abstract: The present invention provides engineered transaminase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered transaminase polypeptides. Methods for producing transaminase enzymes are also provided. In some embodiments, the engineered transaminase polypeptides are optimized to provide improved enzyme properties under minimal aqueous conditions in wet organic solvents.