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39 条记录 (耗时 0.031 秒)

    PANCREATIC ISLET SEPARATION METHOD, AND PROTECTIVE SOLUTION FOR PROTECTING PANCREATIC ISLET TISSUE
    32.
    发明申请
    PANCREATIC ISLET SEPARATION METHOD, AND PROTECTIVE SOLUTION FOR PROTECTING PANCREATIC ISLET TISSUE 有权
    胰岛分离方法和保护胰腺组织的保护性解决方案

    公开(公告)号：US20130177986A1

    公开(公告)日：2013-07-11

    申请号：US13808390

    申请日：2011-07-13

    申请人： Masahiro Tanemura ,  Yoshiki Sawa ,  Akira Myouri ,  Toshinori Ito ,  Masaki Mori ,  Yuichiro Doki

    发明人： Masahiro Tanemura ,  Yoshiki Sawa ,  Akira Myouri ,  Toshinori Ito ,  Masaki Mori ,  Yuichiro Doki

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0676 ,  C12N2501/734

    摘要： The disclosed islet isolation method comprises: an injection step of injecting a preservation solution into the pancreatic duct of an excised pancreas; a preservation step of immersing the pancreas into an immersion fluid for preservation; a digestion step of breaking down the pancreas to provide pancreatic tissue; and a purification step of immersing the pancreatic tissue in a purification solution to provide islets. The digestion step consists of: an enzyme injection step of injecting an enzyme solution containing a digestion enzyme into the pancreas; a digestion initiation step of activating the digestion enzyme; a digestion termination step of inactivating the digestion enzyme; and a collection step of collecting the broken-down pancreatic tissue. The islet isolation method is characterized in that, by adding a neutrophil elastase inhibitor to the system before the digestion initiation step, the neutrophil elastase inhibitor is present inside the pancreas at the time point of starting the digestion initiation step. By using the above method and a protective solution which can be used in the method, islets having a size and shape suitable for transplantation can be obtained in high yields.

    摘要翻译： 公开的胰岛分离方法包括：将保存液注入切除胰腺胰管的注射步骤; 将胰脏浸入用于保存的浸液中的保存步骤; 消化胰腺胰腺组织消化步骤; 以及将胰组织浸入净化溶液中以提供胰岛的纯化步骤。 消化步骤包括：将含有消化酶的酶溶液注入胰腺的酶注射步骤; 活化消化酶的消化引发步骤; 消化终止步骤，使消化酶失活; 以及收集分解的胰组织的收集步骤。 胰岛分离方法的特征在于，通过在消化引发步骤之前向系统中加入嗜中性粒细胞弹性蛋白酶抑制剂，在开始消化起始步骤的时间点，存在嗜中性粒细胞弹性蛋白酶抑制剂在胰腺内。 通过使用上述方法和可用于该方法的保护溶液，可以高产率获得具有适合于移植的尺寸和形状的胰岛。

    Method of producing three-dimensional tissue and method of producing extracellular matrix used in the same
    34.
    发明授权
    Method of producing three-dimensional tissue and method of producing extracellular matrix used in the same 有权
    制备三维组织的方法及其制备方法

    公开(公告)号：US08137964B2

    公开(公告)日：2012-03-20

    申请号：US11514468

    申请日：2006-09-01

    申请人： Mitsuru Akashi ,  Yoshiki Sawa ,  Michiya Matsusaki ,  Yoshio Nakahara

    发明人： Mitsuru Akashi ,  Yoshiki Sawa ,  Michiya Matsusaki ,  Yoshio Nakahara

    IPC分类号： C12N5/06

    CPC分类号： C12N5/0068 ,  C12N5/0062 ,  C12N2533/52 ,  C12N2533/54 ,  Y10S977/902

    摘要： The present invention provides a novel method of producing a three-dimensional tissue by which cell lamination can be carried out easily. According to the method, a three-dimensional tissue in which cell layers are laminated with an extracellular matrix intervening between each pair of the adjacent cell layers is produced by: (A) forming a cell layer on a substrate; (B) bringing the cell layer formed on the substrate into contact with a solution containing a first substance and a solution containing a second substance alternately, thus forming, on the cell layer, an extracellular matrix in which the first substance and the second substance are laminated alternately; and (C) culturing a cell on the extracellular matrix to form a further cell layer. In the present invention, a combination of the first substance and the second substance is (a) a combination of a protein or polymer having an RGD sequence and a protein or polymer that interacts with the protein or polymer having the RGD sequence or (b) a combination of a protein or polymer that is positively charged and a protein or polymer that is negatively charged.

    摘要翻译： 本发明提供一种可以容易地进行细胞层压的三维组织的制造方法。 根据该方法，通过以下方法制造细胞层与介于每对相邻细胞层之间的细胞外基质层叠的三维组织：（A）在基材上形成细胞层; （B）使形成在基板上的细胞层与含有第一物质的溶液和含有第二物质的溶液交替接触，从而在细胞层上形成第一物质和第二物质为细胞外基质 交替叠层; 和（C）在细胞外基质上培养细胞以形成另外的细胞层。 在本发明中，第一物质和第二物质的组合是（a）具有RGD序列的蛋白质或聚合物和与具有RGD序列的蛋白质或聚合物相互作用的蛋白质或聚合物的组合，或（b） 带正电荷的蛋白质或聚合物和带负电荷的蛋白质或聚合物的组合。

    METHOD FOR PRODUCTION OF HEPATIC-LOBULE-LIKE CELL CLUSTER FROM ADIPOSE-TISSUE-DERIVED CELL
    36.
    发明申请
    METHOD FOR PRODUCTION OF HEPATIC-LOBULE-LIKE CELL CLUSTER FROM ADIPOSE-TISSUE-DERIVED CELL 审中-公开
    用于从ADIPOSE-TISSUE-DERIVED细胞中产生肝细胞样细胞簇的方法

    公开(公告)号：US20100183568A1

    公开(公告)日：2010-07-22

    申请号：US12664344

    申请日：2008-06-16

    申请人： Akifumi Matsuyama ,  Hiroshi Komoda ,  Yoshiki Sawa

    发明人： Akifumi Matsuyama ,  Hiroshi Komoda ,  Yoshiki Sawa

    IPC分类号： C12N5/071 ,  A61K35/00 ,  A61K35/407 ,  C12Q1/02 ,  C07K14/00

    CPC分类号： C12N5/0667

    摘要： Disclosed are: a method for producing a hepatic-lobule-like cell mass from an adipose-tissue-derived cell, which is characterized by culturing the adipose-tissue-derived cell; a hepatic-lobule-like cell mass produced by the method; a method for the screening of a substance capable of promoting or inhibiting the formation of a hepatic-lobule-like cell mass, which is characterized by culturing an adipose-tissue-derived cell to produce the hepatic-lobule-like cell mass, wherein a candidate substance is added to a culture medium; and a kit for use in the screening method.

    摘要翻译： 公开了从脂肪组织来源的细胞产生肝小叶状细胞群的方法，其特征在于培养来自脂肪组织的细胞; 通过该方法产生的肝小叶状细胞块; 筛选能够促进或抑制肝小叶状细胞块形成的物质的方法，其特征在于培养脂肪组织来源的细胞以产生肝小叶状细胞块，其中a 将候选物质加入到培养基中; 以及用于筛选方法的试剂盒。

    MULTIPOTENT PROGENITOR CELL DERIVED FROM ADIPOSE TISSUE
    37.
    发明申请
    MULTIPOTENT PROGENITOR CELL DERIVED FROM ADIPOSE TISSUE 有权
    来自ADIPOSE TISSUE的多种原核细胞

    公开(公告)号：US20100151574A1

    公开(公告)日：2010-06-17

    申请号：US12664343

    申请日：2008-06-16

    申请人： Akifumi Matsuyama ,  Hiroshi Komoda ,  Hanayuki Ohkura ,  Yoshiki Sawa

    发明人： Akifumi Matsuyama ,  Hiroshi Komoda ,  Hanayuki Ohkura ,  Yoshiki Sawa

    IPC分类号： C12N5/077

    CPC分类号： C12N5/0667

    摘要： Disclosed is a cell mass containing an adipose-tissue-derived multipotent progenitor cell. Also disclosed is a method for producing an adipose-tissue-derived multipotent progenitor cell from an adipose tissue, which comprises the steps of: (a) removing erythrocytes from an adipose-tissue-derived cell mass to produce a preadipose-tissue-derived multipotent progenitor cell mass; and (b) removing cells other than the adipose-tissue-derived multipotent progenitor cell from the preadipose-tissue-derived multipotent progenitor cell mass to produce the desired adipose-tissue-derived multipotent progenitor cell. Further disclosed is an adipose-tissue-derived multipotent progenitor cell produced by the method.

    摘要翻译： 公开了含有脂肪组织衍生的多能祖细胞的细胞团。 还公开了从脂肪组织制备脂肪组织衍生的多能祖细胞的方法，其包括以下步骤：（a）从脂肪组织衍生的细胞块中除去红细胞，以产生前脂组织衍生的多能 祖细胞; 和（b）除去来自前脂肪组织衍生的多能祖细胞的脂肪组织衍生的多能祖细胞以外的细胞，以产生所需的脂肪组织衍生的多能祖细胞。 进一步公开的是通过该方法生产的脂肪组织衍生的多能祖细胞。

    Agents for protection from neointimal formation in grafts comprising an nfkappab decoy
    39.
    发明申请
    Agents for protection from neointimal formation in grafts comprising an nfkappab decoy 审中-公开
    用于在包含nfkappab诱饵的移植物中保护新内膜形成的药剂

    公开(公告)号：US20060233815A1

    公开(公告)日：2006-10-19

    申请号：US10528346

    申请日：2002-12-27

    申请人： Yoshiki Sawa

    发明人： Yoshiki Sawa

    IPC分类号： A61K39/00

    CPC分类号： A61K31/711

    摘要： The present invention provides methods for using NFκB decoys to regulate (suppress) transcription activated by NFκB, and to suppress neointimal formation in grafts. Furthermore, the present invention relates to agents for protection from intimal thickening in vascular grafts that comprise NFκB decoys.

    摘要翻译： 本发明提供了使用NFkappaB诱饵来调节（抑制）由NFkappaB激活的转录并抑制移植物中的新内膜形成的方法。 此外，本发明涉及用于保护血管移植物内膜增厚的药剂，其包含NFkappaB诱饵。