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62 条记录 (耗时 0.06 秒)

    Methods for producing members of specific binding pairs
    33.
    发明授权
    Methods for producing members of specific binding pairs 失效
    生产特异性结合对成员的方法

    公开(公告)号：US5858657A

    公开(公告)日：1999-01-12

    申请号：US480006

    申请日：1995-06-07

    申请人： Gregory Paul Winter ,  Kevin Stuart Johnson ,  Andrew David Griffiths ,  Andrew John Hammond Smith

    发明人： Gregory Paul Winter ,  Kevin Stuart Johnson ,  Andrew David Griffiths ,  Andrew John Hammond Smith

    IPC分类号： C07K16/18 ,  C07K16/46 ,  C12Q1/68

    CPC分类号： C07K16/18 ,  C07K16/462 ,  C07K2317/24 ,  C07K2319/41 ,  C07K2319/735

    摘要： The invention provides methods and kits for producing specific binding pairs (sbp) members. Populations of polypeptide chain components of sbp members are combined to form libraries of sbps displayed by secreted replicable genetic display packages (rgdp). At least one of the polypeptide chains is expressed as a fusion with a component of an rgdp which thereby displays that polypeptide chain at the surface of rgdp. At least one population of polypeptide chains is expressed from nucleic acid which is capable of being packaged using a component of an rgdp, whereby the genetic material of rgdps produced encodes a polypeptide chain. The methods enable production of libraries of multimeric sbp members from a very large number of possible combinations. In one embodiment of the invention a method employs "chain shuffling" in the production of sbp members of desired specificity for a counterpart sbp member. Selection procedures are also described.

    摘要翻译： 本发明提供用于产生特异性结合对（sbp）成员的方法和试剂盒。 将sbp成员的多肽链组分的群体组合以形成由分泌的可复制的遗传显示包（rgdp）显示的sbps的文库。 至少一个多肽链表达为与rgdp的组分的融合物，由此在rgdp的表面显示该多肽链。 至少一个多肽链群由能够使用rgdp的组分包装的核酸表达，由此产生的rgdps的遗传物质编码多肽链。 该方法能够从非常大量的可能组合生产多聚体sbp成员的文库。 在本发明的一个实施方案中，一种方法在生产对于对应物质成员的所需特异性的sbp成员中使用“链洗牌”。 还描述了选择程序。

    Structured Peptide Processing
    34.
    发明申请
    Structured Peptide Processing 有权
    结构肽加工

    公开(公告)号：US20120142541A1

    公开(公告)日：2012-06-07

    申请号：US13147257

    申请日：2010-02-04

    申请人： Gregory Paul Winter ,  Christian Heinis ,  Elise Bernard ,  David Loakes ,  Marina Vaysburd

    发明人： Gregory Paul Winter ,  Christian Heinis ,  Elise Bernard ,  David Loakes ,  Marina Vaysburd

    IPC分类号： C40B30/00 ,  C40B40/10

    CPC分类号： C12N15/1037 ,  A61K47/55 ,  A61K47/64 ,  A61K47/643 ,  C07K14/001 ,  C12N15/1044 ,  C12N15/1058 ,  C40B40/08 ,  C40B40/10 ,  C40B50/06 ,  G01N33/531

    摘要： The invention relates to a method for modifying one or more peptide ligands, comprising polypeptides covalently linked to a molecular scaffold at two or more amino acid residues, comprising the steps of providing one or more peptide ligands, wherein the polypeptide comprises two or more reactive groups which form a covalent linkage to the molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; exposing the peptide ligands to one or more proteases; and sorting the ligands according to the extent of proteolytic cleavage.

    摘要翻译： 本发明涉及一种修饰一种或多种肽配体的方法，其包括在两个或更多个氨基酸残基处与分子支架共价连接的多肽，包括提供一个或多个肽配体的步骤，其中所述多肽包含两个或多个反应性基团 其形成与分子支架的共价键，以及至少一个环，其包含在两个所述反应性基团之间对位的两个或更多个氨基酸的序列; 将肽配体暴露于一种或多种蛋白酶; 并根据蛋白水解切割的程度对配体进行分选。

    Retargetting antibodies
    38.
    发明授权
    Retargetting antibodies 失效
    重定位抗体

    公开(公告)号：US06589527B1

    公开(公告)日：2003-07-08

    申请号：US08621038

    申请日：1996-03-22

    申请人： Gregory Paul Winter ,  Kaspar Philipp Holliger

    发明人： Gregory Paul Winter ,  Kaspar Philipp Holliger

    IPC分类号： A61K39395

    CPC分类号： C12N15/113 ,  A61K38/00 ,  C07K16/00 ,  C07K16/468 ,  C07K2317/732 ,  C07K2319/00 ,  C12N2310/1241 ,  C12N2310/127

    摘要： Antibodies are retargeted to a target for which they have no functional specificity under normal circumstances. Use is made of a multi-specific binding substance which has binding specificity for the target and anti-antibody binding specificity. The binding substance may comprise an immunoglobulin antigen binding site and may be a “diabody”. Depending on the antibody bound, effector functions such as Complement, ADCC and immune blocking are recruited to act on the target. Example targets are human cells. In vivo and in vitro utilities are exemplified, including lysis of tumor cells and agglutination of red blood cells.

    摘要翻译： 将抗体重新定位到在正常情况下它们没有功能特异性的靶标。 使用具有对靶标具有结合特异性和抗 - 抗体结合特异性的多特异性结合物质。 结合物质可以包含免疫球蛋白抗原结合位点，并且可以是“双抗体”。 根据所结合的抗体，招募诸如补体，ADCC和免疫阻断的效应子功能作用于靶标。 示例目标是人类细胞。 体内和体外实用性被列举，包括肿瘤细胞的溶解和红细胞的凝集。