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88 条记录 (耗时 0.028 秒)

    Nucleotide sequences which code for the sahH gene
    31.
    发明授权
    Nucleotide sequences which code for the sahH gene 有权
    编码sahH基因的核苷酸序列

    公开(公告)号：US06759224B2

    公开(公告)日：2004-07-06

    申请号：US09919854

    申请日：2001-08-02

    申请人： Mike Farwick ,  Klaus Huthmacher ,  Jennifer Brehme ,  Walter Pfefferle ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

    发明人： Mike Farwick ,  Klaus Huthmacher ,  Jennifer Brehme ,  Walter Pfefferle ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

    IPC分类号： C12N914

    CPC分类号： C12N9/88 ,  A23K10/12 ,  A23K20/142 ,  A23K40/35 ,  A23K50/10 ,  C12P13/08 ,  C12P13/12

    摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria, in which at least the sahH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes.

    摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 多肽，其包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列，c）与a）或b）的多核苷酸互补的多核苷酸，和d）包含至少15个连续的多核苷酸的多核苷酸 a），b）或c）的多核苷酸序列的核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少sahH基因以增强形式存在，以及使用多核苷酸 含有根据本发明的序列作为杂交探针。

    Mutant E. coli kiz strains for production of pantothenic acid
    32.
    发明授权
    Mutant E. coli kiz strains for production of pantothenic acid 有权
    用于生产泛酸的突变大肠杆菌kiz菌株

    公开(公告)号：US06171845B2

    公开(公告)日：2001-01-09

    申请号：US09416756

    申请日：1999-10-12

    申请人： Frank Elischweski ,  Jorn Kalinowski ,  Alfred Puhler ,  Nicole Dusch ,  Jurgen Dohmen ,  Mike Farwick ,  Georg Thierbach

    发明人： Frank Elischweski ,  Jorn Kalinowski ,  Alfred Puhler ,  Nicole Dusch ,  Jurgen Dohmen ,  Mike Farwick ,  Georg Thierbach

    IPC分类号： C12N121

    CPC分类号： C12N9/0008 ,  C12P7/42

    摘要： The invention relates to a process for the preparation and improvement of D-pantothenic acid-producing microorganisms by amplification of nucleotide sequences which code for ketopantoate reductase, in particular the panE gene, individually or in combination with one another, and optionally additionally of the ilvC gene, the microorganisms containing these nucleotide sequences, and a process for the preparation of D-pantothenic acid comprising fermentation of these microorganisms, concentration of pantothenic acid in the medium or in the cells of the microorganisms, and isolation of the D-pantothenic acid.

    摘要翻译： 本发明涉及通过扩增编码酮戊酸还原酶，特别是panE基因的核苷酸序列来单独地或彼此组合并任选地另外添加ilvC来制备和改进D-泛酸产生微生物的方法 基因，含有这些核苷酸序列的微生物，以及D-泛酸的制备方法，其包括这些微生物的发酵，在培养基中或微生物细胞中浓缩泛酸，以及分离D-泛酸。

    Nucleotide sequences for encoding of the lysR2-gene
    35.
    发明授权
    Nucleotide sequences for encoding of the lysR2-gene 失效
    编码lysR2基因的核苷酸序列

    公开(公告)号：US07416863B2

    公开(公告)日：2008-08-26

    申请号：US11178485

    申请日：2005-07-12

    申请人： Bettina Moeckel ,  Mike Farwick ,  Thomas Hermann ,  Caroline Kreutzer ,  Walter Pfefferle

    发明人： Bettina Moeckel ,  Mike Farwick ,  Thomas Hermann ,  Caroline Kreutzer ,  Walter Pfefferle

    IPC分类号： C12P19/34

    CPC分类号： C12P13/08 ,  C07K14/34

    摘要： Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c),and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.

    摘要翻译： 分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸，b）多核苷酸， 其编码多肽，其包含与SEQ ID No.2的氨基酸序列至少70％的程度相同的氨基酸序列，c）与a）或b）的多核苷酸互补的多核苷酸， 和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少lysR2基因存在于减毒 形式，以及使用多核苷酸序列作为杂交探针。

    Nucleotide sequences which code for the msiK gene
    37.
    发明授权
    Nucleotide sequences which code for the msiK gene 有权
    编码msiK基因的核苷酸序列

    公开(公告)号：US07252977B2

    公开(公告)日：2007-08-07

    申请号：US09962618

    申请日：2001-09-26

    申请人： Brigitte Bathe ,  Natalie Schischka ,  Mike Farwick ,  Walter Pfefferle

    发明人： Brigitte Bathe ,  Natalie Schischka ,  Mike Farwick ,  Walter Pfefferle

    IPC分类号： C12P13/04 ,  C12P13/10 ,  C12N15/00 ,  C12N1/20 ,  C07H21/02 ,  C07K1/00

    CPC分类号： C12P13/08 ,  C07K14/34

    摘要： The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which encodes the sugar import protein K (msiK) gene, and a host-vector system having a coryneform host bacterium in which the msiK gene is present in enhanced form and a vector which carries at least the msiK gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

    摘要翻译： 本发明涉及具有编码糖进入蛋白K（msiK）基因的多核苷酸序列的谷氨酸棒杆菌的分离的多核苷酸，以及具有其中以增强形式存在msiK基因的棒状细胞宿主细菌的宿主 - 载体系统和载体 其至少携带根据SEQ ID No 1的msiK基因，以及使用包含根据本发明的序列的多核苷酸作为杂交探针。

    Nucleotide sequences coding for the sigM gene
    39.
    发明授权
    Nucleotide sequences coding for the sigM gene 失效
    编码sigM基因的核苷酸序列

    公开(公告)号：US06995000B2

    公开(公告)日：2006-02-07

    申请号：US09942935

    申请日：2001-08-31

    申请人： Brigitte Bathe ,  Christine Bastuck ,  Mike Farwick ,  Thomas Hermann ,  Walter Pfefferle

    发明人： Brigitte Bathe ,  Christine Bastuck ,  Mike Farwick ,  Thomas Hermann ,  Walter Pfefferle

    IPC分类号： C12P13/04

    CPC分类号： C07K14/34 ,  C12P13/08

    摘要： The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which encodes the sigma factor M (sigM) gene, and a host-vector system having a coryneform host bacterium in which the sigM gene is present in enhanced form and a vector which carries at least the sigM gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

    摘要翻译： 本发明涉及具有编码σ因子M（sigM）基因的多核苷酸序列的谷氨酸棒杆菌分离的多核苷酸，以及具有其中sigM基因以增强形式存在的棒状细胞宿主细菌的宿主 - 载体系统和载体， 至少携带根据SEQ ID No 1的sigM基因，以及使用包含根据本发明的序列的多核苷酸作为杂交探针。

    Nucleotide sequences which code for the menE gene
    40.
    发明授权
    Nucleotide sequences which code for the menE gene 失效
    编码menE基因的核苷酸序列

    公开(公告)号：US06946271B2

    公开(公告)日：2005-09-20

    申请号：US09834722

    申请日：2001-04-16

    申请人： Mike Farwick ,  Klaus Huthmacher ,  Achim Marx ,  Walter Pfefferle

    发明人： Mike Farwick ,  Klaus Huthmacher ,  Achim Marx ,  Walter Pfefferle

    IPC分类号： C12N9/00 ,  C12P13/08

    CPC分类号： C12N9/93 ,  C12P13/08 ,  C12Y602/01026

    摘要： The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the menE gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

    摘要翻译： 本发明涉及一种分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）与编码多肽的多核苷酸的程度相同的多核苷酸，所述多核苷酸包含SEQ ID NO： 2，b）编码多肽的多核苷酸，其包含与至少70％与SEQ ID No.2的氨基酸序列相同程度的氨基酸序列，c）多核苷酸，其与 ）或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少所述menE 基因以减毒形式存在，并且使用包含根据本发明的序列的多核苷酸作为杂交探针。