公开(公告)号：US10752908B2

公开(公告)日：2020-08-25

申请号：US16242887

申请日：2019-01-08

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Xudong Ye ,  Larry A. Gilbertson ,  Michael W. Petersen

IPC: C12N15/84 ,  C12R1/41 ,  C12N15/82

Abstract: Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.

公开(公告)号：US20190194681A1

公开(公告)日：2019-06-27

申请号：US16221346

申请日：2018-12-14

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Larry A. Gilbertson ,  Jonathan C. Lamb ,  Jason Ward ,  Jianping Xu

IPC: C12N15/82

CPC classification number: C12N15/8261 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8216

Abstract: Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.

公开(公告)号：US10202611B2

公开(公告)日：2019-02-12

申请号：US14642674

申请日：2015-03-09

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Xudong Ye ,  Larry A. Gilbertson ,  Michael W. Petersen

IPC: C12N15/84 ,  C12N15/82

Abstract: Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.

公开(公告)号：US10066235B2

公开(公告)日：2018-09-04

申请号：US15258901

申请日：2016-09-07

Applicant: Monsanto Technology LLC

Inventor： Larry A. Gilbertson ,  Xudong Ye

IPC: C12N15/84 ,  C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8202

Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.

公开(公告)号：US20170342426A1

公开(公告)日：2017-11-30

申请号：US15670668

申请日：2017-08-07

Applicant: Monsanto Technology LLC

Inventor： Toni Armstrong ,  Kenneth Barton ,  Laura J. Crow ,  Larry A. Gilbertson ,  Shihshieh Huang ,  Yong Huang ,  Brian J. Martinell ,  Michael W. Petersen ,  Kenneth J. Piller ,  Qi Wang ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8209 ,  C12N15/8263 ,  C12N15/8265 ,  C12N15/8289

Abstract: The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.

公开(公告)号：US20170009246A1

公开(公告)日：2017-01-12

申请号：US15258901

申请日：2016-09-07

Applicant: Monsanto Technology LLC

Inventor： Larry A. Gilbertson ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8202

Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.

Abstract translation: 本发明涉及通过使用与重组构建体上的T-DNA边界序列有效连接的其它转化增强子序列（例如过度驱动或TSS序列）来提高农杆菌和根瘤土壤介导的植物细胞转化效率的方法和组合物 其包含T-DNA。

公开(公告)号：US09340797B2

公开(公告)日：2016-05-17

申请号：US13783125

申请日：2013-03-01

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Larry A. Gilbertson ,  David K. Kovalic ,  Thomas J. LaRosa ,  Maolong Lu ,  Tichafa R. I. Munyikwa ,  James K. Roberts ,  Wei Wu ,  Bei Zhang

IPC: C12N15/82 ,  C12N15/87 ,  A01H1/00 ,  A01N63/02

CPC classification number: C12N15/8286 ,  A01N63/02 ,  C12N15/8275 ,  C12N15/8279 ,  C12N15/8285 ,  Y02A40/162 ,  Y02A40/164

Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

公开(公告)号：US20160032300A1

公开(公告)日：2016-02-04

申请号：US14853715

申请日：2015-09-14

Applicant: Monsanto Technology LLC

Inventor： Edwards Allen ,  Larry A. Gilbertson ,  James K. Roberts ,  Nancy M. Houmard ,  Shihshieh Huang ,  Sergey Ivashuta

IPC: C12N15/82

CPC classification number: C12N15/8218 ,  A01H1/02 ,  A01H5/00 ,  A01H5/10 ,  C12N15/8287 ,  C12N15/8289 ,  C12N15/829

Abstract: This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.

公开(公告)号：US20150232865A1

公开(公告)日：2015-08-20

申请号：US14642674

申请日：2015-03-09

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Xudong Ye ,  Larry A. Gilbertson ,  Michael W. Petersen

IPC: C12N15/82

CPC classification number: C12N15/8205

Abstract: Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.

Abstract translation: 提供了用于改善细菌介导的植物转化的方法和组合物。 这些方法通常允许具有减少的载体骨架整合和高频率的低拷贝转化事件的植物转化。 描述了用于实现这些结果的载体，以及它们使用的方法。

公开(公告)号：US20150184179A1

公开(公告)日：2015-07-02

申请号：US14582303

申请日：2014-12-24

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Edwards M. Allen ,  Larry A. Gilbertson ,  Sara E. Heisel ,  Shihshieh Huang ,  Elysia K. Krieger ,  Thomas M. Malvar

IPC: C12N15/82 ,  C12N15/113

CPC classification number: C12N15/8218 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8286 ,  C12N2310/113 ,  Y02A40/162

Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

Abstract translation: 本发明提供了分子构建体及其使用方法，包括异源miRNA识别位点的构建体，用于基因抑制的构建体，包括嵌入在非蛋白质编码序列的一侧或两侧的内含子嵌入的基因抑制元件，含有 工程miRNA或miRNA前体，以及用于抑制细胞中成熟微小RNA的产生的构建体。 还提供了含有这种构建体的转基因植物细胞，植物和种子及其使用的方法。 本发明还提供了转基因植物细胞，植物和种子，其含有重组DNA，用于靶序列的配体受控表达，其可以是内源的或外源的。 还公开了来自作物植物（包括玉米和大豆）的新miRNA和miRNA前体。