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公开(公告)号：US4794073A
公开(公告)日：1988-12-27
申请号：US753734
申请日：1985-07-10
IPC分类号： C12Q1/28 , C12Q1/68 , G01N33/533 , C07H21/00
CPC分类号： G01N33/533 , C12Q1/28 , C12Q1/6816
摘要： A nucleic acid probe capable of participating in a chemiluminescent reaction comprising a defined nucleic acid sequence, the sequence being linked to any one ofa. a chemiluminescence precursor,b. a chemiluminescence enhancer, andc. an enzyme the remaining two of (a), (b) and (c) not linked to the sequence being in a mixture of the linked sequence. A method for determining a particular single stranded polynucleotide sequence in a test medium, comprising the steps of:(a) combining the test medium with a polynucleotide probe having a base sequence substantially complementary to the sequence to be determined,(b) labeling either the resulting hybrids or probe which has not hybridized with the sequence to be determined with one of the participants in an enhanced chamiluminescent reaction involving a chemiluminescent precursor, an enzyme, an oxidant, and a chemiluminescence enhancer,(c) initiating such chemiluminent reaction with the labeled hybrid or probe, and(d) detecting the resulting light emission.
摘要翻译： 能够参与化学发光反应的核酸探针，其包含限定的核酸序列，所述序列与a的任一个连接。 化学发光前体，b。 化学发光增强剂，和c。 （a），（b）和（c）的剩余两个与连接序列的混合物中的序列没有连接的酶。 一种用于在测试培养基中测定特定单链多核苷酸序列的方法，包括以下步骤：（a）将测试培养基与具有与待测序列基本上互补的碱基序列的多核苷酸探针合并，（b） 所产生的杂交体或探针，其未与与参与者之一的待测序列杂交，所述参与者涉及化学发光前体，酶，氧化剂和化学发光增强剂的增强的倒立发光反应，（c）开始与标记的化学发光反应 杂交或探针，和（d）检测所得到的光发射。
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公开(公告)号：US4748111A
公开(公告)日：1988-05-31
申请号：US588858
申请日：1984-03-12
IPC分类号： A61K39/00 , C07K1/113 , C07K14/00 , C07K14/195 , C07K14/41 , C07K16/00 , C07K19/00 , C12Q1/68 , G01N33/531 , G01N33/532 , G01N33/53 , G01N33/543
CPC分类号： C12Q1/682 , G01N33/532 , Y10S435/964 , Y10S436/828
摘要： A protein is covalently coupled to a 3'terminal end of a nucleic acid which carries several labels. In an assay the protein will specifically recognize some component of a test system; in an immunoassay the protein can be Protein A which will recognize the FC portion of IgG which is bound to an unknown antigen if present in the test sample.
摘要翻译： 蛋白质共价连接到携带几个标签的核酸的3'末端。 在测定中，蛋白质将特异性识别测试系统的某些组分; 在免疫测定中，蛋白质可以是蛋白质A，其将识别与测试样品中存在的未知抗原结合的IgG的FC部分。
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33.发明授权Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays 失效标记核酸的快速光化学方法用于杂交测定中的检测目的
公开(公告)号：US4737454A
公开(公告)日：1988-04-12
申请号：US611668
申请日：1984-05-18
CPC分类号： C07H21/00 , C12Q1/6816 , G01N33/533
摘要： A labeled nucleic acid probe comprising (a) a nucleic acid component, (b) a nucleic acid-binding ligand photochemically linked to the nucleic acid component, and (c) a label chemically linked to the nucleic acid-binding ligand. The label can be a specifically bindable ligand such as a hapten or biotin, an enzyme such as a .beta.-galactosidase or horse radish peroxidase, a fluorescent radical, a phycobiliprotein, a luminescent radical, or a radioisotope. The probe can be used in assays of nucleic acids, taking advantage of the ability of the nucleic acid component to hybridize.
摘要翻译： 一种标记的核酸探针，其包含（a）核酸组分，（b）与所述核酸组分光化学连接的核酸结合配体，和（c）与所述核酸结合配体化学连接的标记。 标记可以是特异性结合的配体，例如半抗原或生物素，酶如β-半乳糖苷酶或辣根过氧化物酶，荧光基团，藻胆蛋白，发光基团或放射性同位素。 该探针可用于核酸测定，利用核酸组分杂交的能力。
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公开(公告)号：US4692509A
公开(公告)日：1987-09-08
申请号：US675373
申请日：1984-11-27
IPC分类号： C12Q1/68 , G01N33/534 , G01N33/564 , A61K43/00 , A61N5/12
CPC分类号： G01N33/534 , C12Q1/68 , G01N33/564 , Y10S436/804 , Y10S436/828 , Y10S530/868
摘要： A radioactively labeled protein comprising a protein, and a radioactive nucleoside or nucleotide, the protein being covalently linked to the nucleoside or nucleotide. Advantageously the linkage is through an NH.sub.2 group of the protein and through a carbonyl group of a ring-opened sugar moiety of the nucleoside or nucleotide. The protein can be insulin, an immunoglobulin or protein A. The radioactive moiety may be a P, C, S, H, I or Hg atom. The labels can be used to indicate the presence and amount of the protein in a biological assay.
摘要翻译： 包含蛋白质和放射性核苷或核苷酸的放射性标记的蛋白质，所述蛋白质与核苷或核苷酸共价连接。 有利地，连接通过蛋白质的NH 2基团并通过核苷或核苷酸的开环糖部分的羰基。 蛋白质可以是胰岛素，免疫球蛋白或蛋白质A.放射性部分可以是P，C，S，H，I或Hg原子。 标签可用于指示生物测定中蛋白质的存在和量。
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公开(公告)号：US4670380A
公开(公告)日：1987-06-02
申请号：US612984
申请日：1984-05-23
CPC分类号： C12Q1/6813 , C12Q1/6816
摘要： In a method for determining a particular polynucleotide sequence in a test medium containing single stranded nucleic acids wherein the sample is subjected to a hybridization reaction with a labeled detection probe having a substantially complementary polynucleotide sequence, and wherein after hybridization the label in said probe is assayed, the improvement wherein the label in said labeled probe comprises a fluorescent nucleotide which is linked by a phosphate ester linkage to said probe. Probes and kits therefor are also provided.
摘要翻译： 在用于测定含有单链核酸的测试培养基中的特定多核苷酸序列的方法中，其中样品与具有基本上互补的多核苷酸序列的标记的检测探针进行杂交反应，并且其中杂交后，测定所述探针中的标记 ，其中所述标记的探针中的标记包含通过磷酸酯键与所述探针连接的荧光核苷酸的改进。 还提供了探针和试剂盒。
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36.发明申请Devices and Methods of Anonymously Deconvoluting Combined Patient Samples And Combined Patient Assays 审中-公开匿名解卷积组合患者样品和组合患者测定的设备和方法公开(公告)号：US20110065589A1
公开(公告)日：2011-03-17
申请号：US12518006
申请日：2007-12-13
摘要： Individual patient- and disease-specific test results are obtained from a mixture of one or more distinct tests from multiple combined and distinct patient samples or reactions. In an especially preferred aspect, multiple reaction products from oligonucleotides having unique identifier portions are prepared or received from a clinician and combined for hybridization on a chip. Test results are deconvoluted using a deconvolution table in which associative data are employed to provide access to the individual patient- and disease-specific test results.
摘要翻译： 个体患者和疾病特异性测试结果是从多个组合和不同的患者样品或反应的一个或多个不同测试的混合物获得的。 在特别优选的方面，来自具有唯一标识符部分的寡核苷酸的多种反应产物从临床医生制备或接受并合并用于在芯片上进行杂交。 使用反卷积表对测试结果进行解卷积，其中使用相关数据来提供对个体患者和疾病特异性测试结果的访问。
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公开(公告)号：US06596489B2
公开(公告)日：2003-07-22
申请号：US09823634
申请日：2001-03-30
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q2565/101 , C12Q2521/301 , C12Q2521/113 , C12Q2525/301 , C12Q2523/301
摘要： This invention relates generally to nucleic acid hybridization analysis. More specifically, a method for detecting a point mutation in a DNA strand is provided, which method uses, inter alia, a test nucleic acid strand complementary to a target DNA strand, said nucleic acid strand comprises a sufficient number of ribonucleotide residues that span the position of said point mutation to be detected to form a target DNA strand/test nucleic acid strand duplex and RNase H cleavage of said target DNA strand/test nucleic acid strand duplex. Kits and arrays for detecting a point mutation in a DNA strand comprising test nucleic acid strand comprising a sufficient number of ribonucleotide residues that span the position of said point mutation to be detected are also provided.
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公开(公告)号：US06448047B2
公开(公告)日：2002-09-10
申请号：US09846603
申请日：2001-04-30
IPC分类号： C12P1564
CPC分类号： C12N15/1003
摘要： The present invention describes compositions and methods for releasing nucleic acids from cells in a form that is suitable for labeling/capture, amplification, or detection in a single reagent addition step. The compositions include a lipid, membrane fluidizing compound, enzyme for degrading cell structure, metal chelators, or one or more nucleic acid probes or primers complementary to the nucleic acid to be detected. The compositions are non-denaturing and non-inhibitory of enzymes or proteins that are used in nucleic acid release, amplification, labeling or detection. The invention also provides kits for performing the above methods.
摘要翻译： 本发明描述了以适合于在单个试剂添加步骤中进行标记/捕获，扩增或检测的形式从细胞中释放核酸的组合物和方法。 组合物包括脂质，膜流化化合物，降解细胞结构的酶，金属螯合剂或与待检测的核酸互补的一个或多个核酸探针或引物。 所述组合物是用于核酸释放，扩增，标记或检测的酶或蛋白质的非变性和非抑制性的。 本发明还提供了用于执行上述方法的试剂盒。
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公开(公告)号：US06242188B1
公开(公告)日：2001-06-05
申请号：US09385624
申请日：1999-08-26
IPC分类号： C12Q168
CPC分类号： C12N15/1003
摘要： The present invention describes compositions and methods for releasing nucleic acids from cells in a form that is suitable for labeling/capture, amplification, or detection in a single reagent addition step. The compositions include a lipid, membrane fluidizing compound, enzyme for degrading cell structure, metal chelators, or one or more nucleic acid probes or primers complementary to the nucleic acid to be detected. The compositions are non-denaturing and non-inhibitory of enzymes or proteins that are used in nucleic acid release, amplification, labeling or detection. The invention also provides kits for performing the above methods.
摘要翻译： 本发明描述了以适合于在单个试剂添加步骤中进行标记/捕获，扩增或检测的形式从细胞中释放核酸的组合物和方法。 组合物包括脂质，膜流化化合物，降解细胞结构的酶，金属螯合剂或与待检测的核酸互补的一个或多个核酸探针或引物。 所述组合物是用于核酸释放，扩增，标记或检测的酶或蛋白质的非变性和非抑制性的。 本发明还提供了用于执行上述方法的试剂盒。
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公开(公告)号：US5945336A
公开(公告)日：1999-08-31
申请号：US975570
申请日：1997-11-21
IPC分类号： A61K38/00 , C12N15/113 , C07H21/00 , C12N5/06
CPC分类号： C12N15/1138 , A61K38/00 , C12N2310/315
摘要： The present invention relates to methods of treating disease-associated cellular proliferation using oligonucleotides. In particular, it relates to the use of oligonulceotides which are substantially complementary to interleukin-6 receptor mRNA sequences. In the form of pharmaceutical compositions, these oligonucleotides are suitable for administration to human subjects for the treatment of abnormal cellular proliferation due to such diseases as cancer, autoimmune disorders and viral infection.
摘要翻译： 本发明涉及使用寡核苷酸治疗疾病相关细胞增殖的方法。 特别地，它涉及与白细胞介素-6受体mRNA序列基本上互补的寡核苷酸的用途。 以药物组合物的形式，这些寡核苷酸适合于给予受试者治疗由于诸如癌症，自身免疫病症和病毒感染等疾病引起的异常细胞增殖。
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