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公开(公告)号：US06365381B2
公开(公告)日：2002-04-02
申请号：US09764315
申请日：2001-01-19
IPC分类号： C12P1324
摘要： The present invention provides a method for industrially advantageously producing (S)-4-hydroxy-2-ketoglutaric acid and for producing compounds which are formed by biosynthesis from the precursor (S)-4-hydroxy-2-ketoglutaric acid, for example, for producing the compounds (2S,4S)-4-hydroxy-L-glutamic acid and (2S,4S)-4-hydroxy-L-proline, using a recombinant microorganism carrying a recombinant DNA harboring the DNA fragment encoding 4(S)-4-hydroxy-2-ketoglutaric acid aldolase gene.
摘要翻译： 本发明提供了一种工业上有利地生产（S）-4-羟基-2-酮戊二酸的方法，并且用于生产由前体（S）-4-羟基-2-酮戊二酸生物合成形成的化合物，例如， 使用携带携带有编码4（S）的DNA片段的重组DNA的重组微生物来制备化合物（2S，4S）-4-羟基-L-谷氨酸和（2S，4S）-4-羟基-L-脯氨酸， -4-羟基-2-酮戊二酸醛缩酶基因。
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公开(公告)号：US06312919B1
公开(公告)日：2001-11-06
申请号：US09101327
申请日：1998-07-07
申请人： Chiaki Saitoh , Hideyo Kumazawa , Kazuo Aisaka , Toru Mizukami , Katsuhiko Ando , Keiko Ochiai , Ryoichi Katsumata , Kozo Ouchi
发明人： Chiaki Saitoh , Hideyo Kumazawa , Kazuo Aisaka , Toru Mizukami , Katsuhiko Ando , Keiko Ochiai , Ryoichi Katsumata , Kozo Ouchi
IPC分类号： C12P3300
CPC分类号： C12Y101/99001 , A23K20/189 , A23L5/25 , A23L13/48 , A23L15/25 , A23L17/65 , C12N9/0006 , C12P33/00
摘要： An enzymatic process is presented for reducing the cholesterol level of foods and feeds by converting the cholesterol therein to coprostanol which has a very low intestinal tract absorbability. Cholesterol in meat, egg, milk, seafood and cooked processed foods containing the same, or feeds for animals, poultry, and pisiculture can be reduced by the sequential action of three enzymes isolated from a eubacterium: cholesterol dehydrogenase, 4-cholesten-3-one dehydrogenase and coprostan-3-one dehydrogenase.
摘要翻译： 提出了一种酶法，用于通过将其中的胆固醇转化成具有非常低的肠道吸收性的coprostanol来降低食物和饲料的胆固醇水平。 肉，蛋，牛奶，海鲜和含有此类食物的熟化加工食品中的胆固醇或动物，家禽和养殖用饲料可以通过从真菌分离的三种酶的顺序作用来降低：胆固醇脱氢酶，4-胆甾醇-3- 一种脱氢酶和副雄甾-3-酮脱氢酶。
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33.发明授权Corynebacterium strain for producing L-tryptophan decreased in phosphoenolpyruvate carboxylase activity 失效用于产生L-色氨酸的棒状杆菌菌株磷酸烯醇丙酮酸羧化酶活性降低
公开(公告)号：US5595906A
公开(公告)日：1997-01-21
申请号：US429472
申请日：1995-04-27
申请人： Ryoichi Katsumata , Kuniki Kino
发明人： Ryoichi Katsumata , Kuniki Kino
CPC分类号： C12P13/22 , Y10S435/84 , Y10S435/843
摘要： The invention relates to a bacterial process for producing L-tryptophan, L-tyrosine or L-phenylalanine. The process utilizes a coryneform glutamic acid-producing bacterium being capable of producing L-tryptophan, L-tyrosine or L-phenylalanine and also decreased or lacked in phosphoenolpyruvate carboxylase activity. The mutant strain is then cultured in order to accumulate the amino acid in a medium and the amino acid is recovered therefrom.
摘要翻译： 本发明涉及生产L-色氨酸，L-酪氨酸或L-苯丙氨酸的细菌方法。 该方法利用产生L-色氨酸，L-酪氨酸或L-苯丙氨酸的棒状谷氨酸生产细菌，并且还降低或缺乏磷酸烯醇丙酮酸羧化酶活性。 然后培养突变菌株以将氨基酸在培养基中积累，并从中回收氨基酸。
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公开(公告)号：US5521074A
公开(公告)日：1996-05-28
申请号：US450079
申请日：1995-05-25
CPC分类号： C12P13/08 , Y10S435/84 , Y10S435/843
摘要： There is provided a process for producing L-valine which comprises cultivating, in a medium, a microorganism which belongs to the genus Corynebacterium or Brevibacterium, which exhibits a) an ability to produce L-valine, b) resistance to L-valine in a medium containing acetic acid as a sole carbon source, and c) sensitivity to a pyruvic acid analog in a medium containing glucose as a sole carbon source, until L-valine is accumulated in the culture broth, and recovering L-valine therefrom.
摘要翻译： 提供了一种生产L-缬氨酸的方法，其包括在培养基中培养属于棒杆菌属或短杆菌属的微生物，其显示出a）产生L-缬氨酸的能力，b）在L-缬氨酸中对L-缬氨酸的抗性 含有乙酸作为唯一碳源的培养基，以及c）在含有葡萄糖作为唯一碳源的培养基中对丙酮酸类似物的敏感性，直到L-缬氨酸在培养液中积聚，并从其中回收L-缬氨酸。
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公开(公告)号：US5236831A
公开(公告)日：1993-08-17
申请号：US474648
申请日：1990-02-02
申请人： Ryoichi Katsumata , Akio Ozaki , Toru Mizukami , Motoko Kageyama , Morimasa Yagisawa , Tamio Mizukami , Seiga Itoh , Tetsuo Oka , Akira Furuya
发明人： Ryoichi Katsumata , Akio Ozaki , Toru Mizukami , Motoko Kageyama , Morimasa Yagisawa , Tamio Mizukami , Seiga Itoh , Tetsuo Oka , Akira Furuya
摘要： Disclosed is a process for expressing a gene and producing a metabolic product formed by the gene by culturing a transformant microorganism carrying a recombinant DNA constructed of a DNA fragment having at least one gene to be expressed and a vector DNA, at least one of which is foreign to the host microorganism.
摘要翻译： 公开了通过培养携带由具有至少一种待表达基因的DNA片段和载体DNA构建的重组DNA的转化体微生物来表达基因并产生由该基因形成的代谢产物的方法，其中至少一种是 外来宿主微生物。
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公开(公告)号：US4927758A
公开(公告)日：1990-05-22
申请号：US89922
申请日：1987-08-25
申请人： Toru Mizukami , Ryoichi Katsumata , Tetsuo Oka
发明人： Toru Mizukami , Ryoichi Katsumata , Tetsuo Oka
IPC分类号： C12N15/09 , C12N1/21 , C12N15/52 , C12P13/04 , C12P13/06 , C12P13/10 , C12P13/22 , C12P13/24 , C12R1/13 , C12R1/15
CPC分类号： C12P13/04 , C12N15/52 , C12P13/06 , C12P13/10 , C12P13/22 , Y10S435/84 , Y10S435/843
摘要： Disclosed is a process for producing histidine by transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA of a DNA fragment containing a gene involved in the biosynthesis of histidine and a vector DNA, culturing the transformant in a nutrient medium, accumulating histidine in the culture medium and recovering histidine therefrom.
摘要翻译： 公开了通过用包含组氨酸生物合成的基因的DNA片段的重组DNA和载体DNA转化属于棒状杆菌属或短杆菌属的宿主微生物来培养组氨酸的方法，在营养培养基中培养转化体， 组氨酸并从其中回收组氨酸。
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公开(公告)号：US4681847A
公开(公告)日：1987-07-21
申请号：US695574
申请日：1985-01-28
申请人： Ryoichi Katsumata , Tetsuo Oka , Akira Furuya
发明人： Ryoichi Katsumata , Tetsuo Oka , Akira Furuya
IPC分类号： C12N15/77 , C12N15/00 , C12N1/00 , C12N1/06 , C12N1/20 , C12N9/36 , C12P19/34 , C12P21/00 , C12R1/13 , C12R1/15
CPC分类号： C12R1/13 , C12N15/77 , C12R1/15 , Y10S435/84 , Y10S435/843
摘要： A novel lysozyme-sensitive microorganism belonging to the genus Corynebacterium or Brevibacterium and having a sensitivity to lysozyme at a concentration of less than 25 .mu.g/ml is provided from selected mutants. This novel microorganism is especially suitable for use in recombinant DNA technology.
摘要翻译： 从选择的突变体提供属于棒杆菌属或短杆菌属的新型溶菌酶敏感性微生物，其浓度低于25μg/ ml对溶菌酶具有敏感性。 该新型微生物特别适用于重组DNA技术。
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