公开(公告)号：US06692958B2

公开(公告)日：2004-02-17

申请号：US10003539

申请日：2001-12-04

申请人： Teruaki Sekine ,  Kiminari Ito ,  Norio Shimizu ,  Kenzo Bamba ,  Tomohiro Yamaguchi ,  Yasuyuki Kuroiwa

发明人： Teruaki Sekine ,  Kiminari Ito ,  Norio Shimizu ,  Kenzo Bamba ,  Tomohiro Yamaguchi ,  Yasuyuki Kuroiwa

IPC分类号： C12N508

CPC分类号： C12N5/0636 ,  A61K2039/5158 ,  C12N2501/23 ,  C12N2501/515

摘要： Activated lymphocytes derived from cord blood are excellently effective for preventing and treating various types of tumors and various types of infection. With interleukin 2 and/or anti-CD3 antibody, the lymphocytes derived from the cord blood is prepared by segregating lymphocytes from the cord blood and proliferating the segregated lymphocytes directly in vitro or segregating monocytes from cord blood and proliferating the monocytes in vitro. Also, the cord blood-derived activated lymphocytes can be effectively used for preventing recurrence of the diseases and promoting the take of stem cells or other organs.

摘要翻译： 来自脐带血的活化淋巴细胞对于预防和治疗各种类型的肿瘤和各种​​感染是非常有效的。 通过白细胞介素2和/或抗CD3抗体，通过从脐带血分离淋巴细胞并且在体外直接增殖分离的淋巴细胞或从体外将脐带血分离单核细胞并增殖单核细胞来制备来自脐带血的淋巴细胞。 此外，脐带血衍生的活化淋巴细胞可以有效地用于预防疾病的复发和促进干细胞或其他器官的摄入。

公开(公告)号：US5902489A

公开(公告)日：1999-05-11

申请号：US745656

申请日：1996-11-08

申请人： Kenji Yasuda ,  Shin-ichiro Umemura ,  Kazuo Takeda ,  Mitsuru Tamura ,  Norio Shimizu

发明人： Kenji Yasuda ,  Shin-ichiro Umemura ,  Kazuo Takeda ,  Mitsuru Tamura ,  Norio Shimizu

IPC分类号： G01N1/36 ,  B01D21/00 ,  B01D43/00 ,  B01J19/10 ,  B01D17/06 ,  B01D35/06

CPC分类号： B01J19/10 ,  B01D21/283 ,  B01D21/34 ,  B01D43/00

摘要： A chamber for exerting an ultrasound beam on a sample solution containing particles to be concentrated, separated or arranged is provided with a view toward arbitrarily controlling the shape of a spatial distribution of potential energy created by the ultrasound beam and concentrating, separating or periodically arranging the particles. In contrast to the chamber, irradiation ultrasound sources for generating ultrasound beams are provided to create an ultrasonic intensity distribution for producing a position potential energy distribution used to exert a force forwarded in a predetermined direction or a force staying at a predetermined region to each particle. Thus, a specific spatial distribution of potential energy can be realized by ultrasound beams each having a specific intensity, a specific frequency and a specific phase or an ultrasound beam formed by superimposing these on one another. Further, at least one suction hole for recovering separated solvents or particles is defined in a wall surface of the tube, which is orthogonal to the flow of the sample solution.

摘要翻译： 在包含要集中，分离或排列的颗粒的样品溶液上施加超声波束的腔室设置有任意控制由超声波束产生的势能空间分布的形状的集中，分离或周期性排列 粒子。 与腔室相反，提供用于产生超声波束的照射超声波源以产生用于产生用于施加沿预定方向传递的力的位置势能分布的超声波强度分布或者保持在每个粒子的预定区域的力。 因此，可以通过每个具有特定强度，特定频率和特定相位的超声波束或通过将它们彼此叠加而形成的超声波束来实现势能的特定空间分布。 此外，在管的壁表面中限定用于回收分离的溶剂或颗粒的至少一个吸孔，其与样品溶液的流动正交。

公开(公告)号：USD359954S

公开(公告)日：1995-07-04

申请号：US20220

申请日：1994-03-22

申请人： Norio Shimizu ,  Teruo Matsuno

设计人： Norio Shimizu ,  Teruo Matsuno

公开(公告)号：USD351827S

公开(公告)日：1994-10-25

申请号：US8696

申请日：1993-05-25

申请人： Norio Shimizu ,  Teruo Matsuno

设计人： Norio Shimizu ,  Teruo Matsuno

公开(公告)号：US5328843A

公开(公告)日：1994-07-12

申请号：US660875

申请日：1991-02-26

申请人： Jun Fukuda ,  Hideo Kawaguchi ,  Takejiro Ushiroda ,  Norio Shimizu ,  Kazuo Sato

发明人： Jun Fukuda ,  Hideo Kawaguchi ,  Takejiro Ushiroda ,  Norio Shimizu ,  Kazuo Sato

IPC分类号： C12M1/00 ,  B01L3/00 ,  C12M3/00 ,  C12N5/07 ,  C12N5/071 ,  C12N5/10 ,  C12N5/06

CPC分类号： B01L3/0293 ,  B01L3/5027 ,  C12M33/00 ,  B01L2300/046 ,  B01L2300/0816 ,  B01L2300/0822 ,  B01L2400/0406

摘要： Apertures in a definite shape having a definite width and length are provided on one surface of a plate material having a definite thickness. One end of the apertures is connected with the other surface of the plate material and at the same time, is opened. Another end of the apertures is connected with the plate material on one surface and is also opened toward the other surface of a culture substrate. The plate material is closely contacted to one surface of the culture substrate for allocating nerve cells and observing growth of the neurites. Then, the cell suspension is supplied to the apertures. The cells in the suspension are allocated on the culture substrate in response to the apertures by centrifugation or spontaneous sedimentation. The plate material is then withdrawn.

摘要翻译： 在具有确定厚度的板材的一个表面上设置具有确定宽度和长度的确定形状的孔。 孔的一端与板材的另一面连接，同时打开。 孔的另一端在一个表面上与板材连接，并且朝向培养基底的另一表面打开。 板材与培养基质的一个表面紧密接触，用于分配神经细胞并观察神经突生长。 然后，将细胞悬浮液提供给孔。 悬浮液中的细胞通过离心或自发沉降响应于孔而分配在培养基底上。 然后取出板材。

公开(公告)号：US5139938A

公开(公告)日：1992-08-18

申请号：US157719

申请日：1988-02-19

申请人： Norio Shimizu ,  Shinichi Fukuzono ,  Kiyoshi Fujimori ,  Nobuko Nishimura ,  Yoji Odawara

发明人： Norio Shimizu ,  Shinichi Fukuzono ,  Kiyoshi Fujimori ,  Nobuko Nishimura ,  Yoji Odawara

IPC分类号： C12M1/36 ,  C12N1/00 ,  C12N1/20 ,  C12P1/00 ,  C12P21/02

CPC分类号： C12M41/32 ,  C12M41/48 ,  C12P1/00 ,  C12P21/02 ,  Y10S435/849

摘要： Cultivation of microorganisms or animal cells or plant cells is carried out to produce high density cultivation, high cell yield and high production of desired metabolite products by monitoring acetate concentration in culture broth and regulating assimilation of acetate in the culture broth by the microorganisms or animal cells or plant cells to control the acetate concentration to a set value or less. Preferably, an acetic acid-producing bacterium that is inhibited by acetic acid and is capable of assimilating acetic acid is cultured to produce biologically active substances such as enzymes. The bacterium may be a recombinant Escherichia coli and an inducer which acts on a promoter in an expression vector is added to produce the desired metabolite product. The inducer is 3-.beta.- indolylacrylic acid when the promoter is trp-promoter or isopropyl-.beta.-D-thiogalactoside when the promoter is lac-promoter or tacpromoter.