公开(公告)号：US20200326327A1

公开(公告)日：2020-10-15

申请号：US16862196

申请日：2020-04-29

Applicant: Ultima Genomics, Inc.

Inventor： Kristopher BARBEE ,  Nathan BECKETT ,  Denis PRISTINSKI ,  Derek SCHULTE ,  Avishai BARTOV ,  Jamie SULLIVAN ,  Dumitru BRINZA ,  Abizar LAKDAWALLA ,  Steven MENCHEN ,  Gilad ALMOGY ,  Mark PRATT

IPC: G01N33/49 ,  G01Q10/04 ,  G01N1/02

Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate configured to rotate. The open substrate can comprise an array of immobilized analytes. A solution comprising a plurality of probes may be directed, via centrifugal force, across the array during rotation of the substrate, to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via continuous rotational area scanning of the substrate.

公开(公告)号：US20190153531A1

公开(公告)日：2019-05-23

申请号：US15974364

申请日：2018-05-08

Applicant: Ultima Genomics, Inc.

Inventor： Kristopher BARBEE ,  Nathan BECKETT ,  Denis PRISTINSKI ,  Derek SCHULTE ,  Avishai BARTOV ,  Jamie SULLIVAN ,  Dumitru BRINZA ,  Abizar LAKDAWALLA ,  Steven MENCHEN ,  Gilad ALMOGY ,  Mark PRATT

IPC: C12Q1/6874 ,  C12Q1/6825

CPC classification number: C12Q1/6874 ,  B01J19/0046 ,  B01J2219/00536 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00689 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L3/5023 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/026 ,  B01L2200/0647 ,  B01L2300/0654 ,  B01L2400/0409 ,  C12Q1/6809 ,  C12Q1/6825 ,  C12Q1/6834 ,  G01N33/54366 ,  C12Q2565/629

Abstract: Provided are methods for biological sample processing and analysis. A method can comprise providing a substrate configured to rotate. The substrate can comprise an array having immobilized thereto a biological analyte. A solution comprising a plurality of probes may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector can be configured to detect a signal from the at least one probe coupled to the biological analyte, thereby analyzing the biological analyte.

公开(公告)号：US20250109429A1

公开(公告)日：2025-04-03

申请号：US18919271

申请日：2024-10-17

Applicant: Ultima Genomics, Inc.

Inventor： Aklilu WORKU ,  Robert ONO ,  Daniel MAZUR ,  Gilad ALMOGY

IPC: C12Q1/6806 ,  C01B33/113 ,  C12Q1/6869

Abstract: Provided herein are methods, systems, and kits for generating a self-assembled monolayer of beads adjacent to (e.g., on or across) a substrate. The methods, systems, and kits provided herein may comprise contacting a substrate with a plurality of beads, which may comprise nucleic acid molecules coupled thereto, and providing conditions sufficient for the plurality of beads to self-assemble adjacent to the substrate. Sequencing of the nucleic acid molecules may be performed.

公开(公告)号：US20230348965A1

公开(公告)日：2023-11-02

申请号：US18132693

申请日：2023-04-10

Applicant: ULTIMA GENOMICS, INC.

Inventor： Gilad ALMOGY ,  Mark PRATT ,  Florian OBERSTRASS

IPC: C12Q1/6869 ,  C12Q1/6806

CPC classification number: C12Q1/6869 ,  C12Q1/6806

Abstract: Recognized herein is the need for methods and processes for increasing the efficiency and accuracy of paired end sequencing.

公开(公告)号：US20230313287A1

公开(公告)日：2023-10-05

申请号：US17991429

申请日：2022-11-21

Applicant: Ultima Genomics, Inc.

Inventor： Gilad ALMOGY ,  Linda LEE

IPC: C12Q1/6869

CPC classification number: C12Q1/6869

Abstract: The present disclosure provides methods and systems for determining a nucleic acid sequence of a target nucleic acid molecule. A method for sequencing a target nucleic acid molecule comprises subjecting a plurality of nucleic acid molecules exhibiting sequence homology to the target nucleic acid molecule to at most 4000 cycles a nucleic acid extension reaction while measuring detectable signals from the plurality of nucleic acid molecules. The detectable signals may correspond to individual nucleotides or nucleotide analogs incorporated into the plurality of nucleic acid molecules during the nucleic acid extension reaction. The detectable signals may be used to generate a sequence of the target nucleic acid molecule at a length of at least about 500 bases and an accuracy of at least about 97%.

公开(公告)号：US20230279486A1

公开(公告)日：2023-09-07

申请号：US18101415

申请日：2023-01-25

Applicant: ULTIMA GENOMICS, INC.

Inventor： Gilad ALMOGY ,  Florian OBERSTRASS

IPC: C12Q1/6869 ,  C12Q1/6876

CPC classification number: C12Q1/6869 ,  C12Q1/6876

Abstract: The present disclosure provides methods and systems for sequencing nucleic acid molecules using a single frequency during detection, or fewer frequencies than types of nucleotide bases identified during detection. Methods and systems of the present disclosure may involve transiently binding nucleotides. Methods and systems provided herein may enable sequences to be determined at a higher accuracy and efficiency.

公开(公告)号：US20230152300A1

公开(公告)日：2023-05-18

申请号：US17962163

申请日：2022-10-07

Applicant: Ultima Genomics, Inc.

Inventor： Kristopher BARBEE ,  Nathan BECKETT ,  Denis PRISTINSKI ,  Derek SCHULTE ,  Avishai BARTOV ,  Jamie SULLIVAN ,  Dumitru BRINZA ,  Abizar LAKDAWALLA ,  Steven MENCHEN ,  Gilad ALMOGY ,  Mark PRATT

IPC: G01N33/49 ,  G01N1/02 ,  G01Q10/04

CPC classification number: G01N33/492 ,  G01N1/02 ,  G01Q10/04 ,  G01N2001/2291

Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate configured to rotate. The open substrate can comprise an array of immobilized analytes. A solution comprising a plurality of probes may be directed, via centrifugal force, across the array during rotation of the substrate, to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via continuous rotational area scanning of the substrate.

公开(公告)号：US20220348994A1

公开(公告)日：2022-11-03

申请号：US17712765

申请日：2022-04-04

Applicant: Ultima Genomics, Inc.

Inventor： Linda LEE ,  Steven MENCHEN ,  Theo NIKIFOROV ,  Gilad ALMOGY ,  Florian OBERSTRASS

IPC: C12Q1/6823 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N21/64

Abstract: The disclosure provides methods for sequencing nucleic acids using, including with nucleotide analogs and subsequently appended labels.

公开(公告)号：US20220199201A1

公开(公告)日：2022-06-23

申请号：US17574260

申请日：2022-01-12

Applicant: ULTIMA GENOMICS, INC.

Inventor： Mark PRATT ,  Gilad ALMOGY ,  Avishai BARTOV

IPC: G16B40/10 ,  G16B30/10 ,  G16B45/00 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: The present disclosure provides methods and systems for accurate and efficient context-aware base calling of sequences. In an aspect, disclosed herein is a method for sequencing a nucleic acid molecule, comprising: (a) sequencing the nucleic acid molecule to generate a plurality of sequence signals; and (b) determining base calls of the nucleic acid molecule based at least in part on (i) the plurality of sequence signals and (ii) quantified context dependency for at least a portion of the plurality of sequence signals.

公开(公告)号：US20220168745A1

公开(公告)日：2022-06-02

申请号：US17673444

申请日：2022-02-16

Applicant: Ultima Genomics, Inc.

Inventor： Jose Martin SOSA ,  Joseph ANTHONY ,  Nathan BECKETT ,  Gilad ALMOGY ,  Chandan SHEE ,  Phillip You Fai LEE ,  Yuto WATANABE

IPC: B01L7/00 ,  B01L3/00

Abstract: The present disclosure provides methods and processes for increasing the efficiency and accuracy of nucleic acid sequencing using techniques such as polymerase chain reaction (PCR). Methods and systems provided herein may facilitate performing reactions such as emulsion PCR (ePCR) on samples comprising nucleic acids and beads. The methods provided herein may provide a higher throughput compared to existing technologies.