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公开(公告)号：US06174669B1
公开(公告)日：2001-01-16
申请号：US08752540
申请日：1996-11-20
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C07B2200/11 , C12N15/1093 , C12N15/1096 , C40B40/00
摘要： Disclosed is a method for making full-length CDNA libraries, which is for making libraries of cDNAs having a length corresponding to a full-length of mRNAs and comprises the following steps of; binding a tag molecule to a diol structure present in 5′ Cap (7MeGpppN) sites of mRNAs, forming RNA-DNA hybrids by reverse transcription using primers such as oligo dT and the mRNAs connected with the tag molecule as templates, and separating RNA-DNA hybrids carrying a DNA corresponding to a full-length of mRNAs from the RNA-DNA hybrids formed above by using function of the tag molecule. To obtain mRNA connected with a tag molecule, the diol structure present in 5′ Cap site of mRNA is subjected to a ring-open reaction by oxidation with sodium periodate to form a dialdehyde and the dialdehyde is reacted with a tag molecule having a hydrazine terminus. According to the present invention, there are provided a novel method capable of efficiently labeling 5′ Cap site and a method for making full-length cDNA libraries utilizing the labeling method.
摘要翻译： 公开了一种制备全长CDNA文库的方法，其用于制备具有对应于全长mRNA的长度的cDNA文库，并且包括以下步骤： 将标签分子与存在于mRNA的5'帽（7MeGpppN）位点的二醇结构结合，通过使用诸如oligo dT的引物和与标签分子连接的mRNA作为模板通过逆转录形成RNA-DNA杂交体，并分离RNA-DNA 通过使用标签分子的功能，携带与来自上述RNA-DNA杂交体的全长mRNA相对应的DNA的杂交体。 为了获得与标签分子连接的mRNA，存在于mRNA的5'帽位点的二醇结构通过用高碘酸钠氧化进行开环反应以形成二醛，并且使二醛与具有肼末端的标签分子反应 。 根据本发明，提供了一种能够利用标记方法有效地标记5'帽位点和制备全长cDNA文库的方法的新方法。
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公开(公告)号：US08097414B2
公开(公告)日：2012-01-17
申请号：US12094896
申请日：2006-11-21
IPC分类号： C12P19/34
CPC分类号： C12Q1/6844 , C12Q1/6865 , C12Q2527/125 , C12Q2525/301
摘要： Problem to be solved There is provided a method for detecting and/or amplifying a nucleic acid contained in a biological sample such as blood or cells conveniently, rapidly, and effectively.Solution There is provided a method for detecting a nucleic acid contained in a sample, comprising the step of adding at least one substance selected from the group consisting of polyphenols, polyhydric alcohols, sugar acids, sugar alcohols, and hydrophilic biodegradable polymers to a sample, the step of complementarily binding an oligonucleotide complementary to a part of the nucleic acid sequence of a nucleic acid to be detected to a part of the nucleic acid sequence, and the step of detecting the nucleic acid to be detected.
摘要翻译： 待解决的问题提供一种方便，快速，有效地检测和/或扩增生物样品如血液或细胞中的核酸的方法。 溶液提供了一种检测样品中所含的核酸的方法，其包括将至少一种选自多酚，多元醇，糖酸，糖醇和亲水性可生物降解聚合物的物质加入到样品中的步骤， 将与要检测的核酸的一部分核酸序列互补的寡核苷酸互补结合到核酸序列的一部分的步骤和检测待检测的核酸的步骤。
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33.发明申请公开(公告)号：US20100221787A1
公开(公告)日：2010-09-02
申请号：US12739496
申请日：2008-10-24
申请人： Yoshihide Hayashizaki , Masayoshi Itoh , Alexander Lezhava , Yoshimi Benno , Yasumasa Mitani , Hajime Kanamori
发明人： Yoshihide Hayashizaki , Masayoshi Itoh , Alexander Lezhava , Yoshimi Benno , Yasumasa Mitani , Hajime Kanamori
CPC分类号： C12Q1/6846 , C12N9/1252 , C12P19/34 , C12Q2525/155 , C12Q2521/107 , C12Q2527/101 , C12Q2531/107
摘要： A DNA polymerase suitable for specific isothermal amplification methods and an isothermal amplification method using the DNA polymerase are provided. In the presence of a DNA polymerase including a protein described in the following item (a) or (b), an amplification reaction of a target nucleic acid sequence in a nucleic acid sample is carried out isothermally using a first primer shown in the following (X). By using the DNA polymerase, it becomes possible to carry out the amplification reaction using the primer within a shorter time than ever before. (a) a protein having an amino acid sequence represented by SEQ ID NO. 23 (b) a protein having an amino acid sequence represented by SEQ ID NO. 25 (X) a primer that contains, in a 3′ end portion, a sequence (Ac′) that hybridizes to a sequence (A) of a 3′ end portion of the target nucleic acid sequence and also contains, on a 5′ side of the sequence (Ac′), a sequence (B′) that hybridizes to a complementary sequence (Bc) to a sequence (B) present on a 5′ side with respect to the sequence (A) in the target nucleic acid sequence
摘要翻译： 提供了适用于特定等温扩增方法的DNA聚合酶和使用DNA聚合酶的等温扩增方法。 在包含下述（a）或（b）中所述的蛋白质的DNA聚合酶的存在下，使用下述第一引物等温进行靶核酸序列的扩增反应 X）。 通过使用DNA聚合酶，可以在比以往更短的时间内使用引物进行扩增反应。 （a）具有SEQ ID NO：1所示的氨基酸序列的蛋白质。 23（b）具有SEQ ID NO：1所示的氨基酸序列的蛋白质。 25（X）引物，其在3'末端含有与目标核酸序列的3'端部分的序列（A）杂交的序列（Ac'），并且在5' 序列（Ac'）侧，与靶序列（A）相对于存在于5'侧的序列（B）的互补序列（Bc）杂交的序列（B'）
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公开(公告)号：US20060110745A1
公开(公告)日：2006-05-25
申请号：US11123223
申请日：2005-05-06
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q2533/101 , C12Q2525/301 , C12Q2521/501 , C12Q2521/101
摘要： The invention provides a sequence specific method for amplifying nucleic acids. More particularly, the invention provides a method for amplifying nucleic acid sequences which enables such sequences to be detected with high precision, rapidity and high specificity as compared to conventional methods. The present invention also provides a simple method for cloning nucleic acids, particularly, a rapid and simple method for amplifying alternative splicing forms synthesized by an alternative splicing which is performed in a process of preparing a matured mRNA from a DNA.
摘要翻译： 本发明提供了用于扩增核酸的序列特异性方法。 更具体地，本发明提供了与常规方法相比，用于扩增能够以高精度，快速和高特异性检测这种序列的核酸序列的方法。 本发明还提供了用于克隆核酸的简单方法，特别是用于扩增通过在从DNA中制备成熟mRNA的过程中进行的选择性剪接合成的可变剪接形式的快速和简单的方法。
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公开(公告)号：US07014745B2
公开(公告)日：2006-03-21
申请号：US10401862
申请日：2003-03-31
IPC分类号： G01N27/453 , G01N30/02
CPC分类号： G01N27/44782 , Y10S83/95 , Y10T29/5148 , Y10T29/53391 , Y10T83/0596
摘要： Capillary columns (102) pass through and are inserted in a rubber plate (14), held and fixed by elastic force of rubber, and two-dimensionally arranged on a sample injection side. It fixes the capillary columns (102) arranged on a plane in close contact by holding the same with a holder plate (6a) from below and with a rubber plate (16) from above on a detection side. In order to press the capillary columns (102) against the holder plate 6a and fix the same with the rubber plate (16), a holder plate (6b) fixing the rubber plate (16) to the holder plate (6a) on both sides of the arrangement of the capillary columns (102) is provided.
摘要翻译： 毛细管柱（102）穿过并被插入橡胶板（14）中，橡胶板通过橡胶的弹力保持和固定，并且二维地布置在样品注射侧。 它通过将保持板（6a）从下方夹持，并在检测侧从上方与橡胶板（16）固定在紧密接触的布置在平面上的毛细管柱（102）。 为了将毛细管柱（102）压靠在保持板6a上并将其固定在橡胶板（16）上，将橡胶板（16）固定到保持板（6a）上的保持板（6b） 在毛细管柱（102）的布置的两侧。
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36.发明申请PRINTED MATERIALS COMPRISING A SUPPORT HAVING AN OLIGOMER AND/OR A POLYMER APPLIED THEREON, A METHOD FOR PREPARING THE SAME AND A METHOD FOR DELIVERING AND/OR STORING THE SAME 审中-公开包含具有低聚物和/或其中的聚合物的支持物的印刷材料，其制备方法和用于交付和/或存储该聚合物的方法公开(公告)号：US20050166782A2
公开(公告)日：2005-08-04
申请号：US10490519
申请日：2004-03-23
IPC分类号： B42D11/00 , B01J19/00 , B42D15/00 , C07B61/00 , C07K14/59 , C12N15/00 , C12N15/09 , B41F1/00
CPC分类号： B01J19/0046 , B01J2219/00373 , B01J2219/00378 , B01J2219/00387 , B01J2219/00527 , B01J2219/00547 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01J2219/00731 , B42D15/0086 , C07B2200/11 , C07K14/59 , C40B40/00
摘要： Abstract of the DisclosureA printed material comprising at least one support having at least one oligomer and/or polymer applied thereon is provided. Also, a method for preparing the printed material and a method for delivering and storing at least one oligomer and/or polymer are provided. The printed materials of the present invention are useful in providing scientists with oligomers and/or polymers of interest from the printed materials easily and immediately.
摘要翻译： 公开摘要提供了包含至少一种具有至少一种低聚物和/或聚合物的载体的印刷材料。 此外，提供了制备印刷材料的方法和用于递送和存储至少一种低聚物和/或聚合物的方法。 本发明的印刷材料可用于向科学家提供易于且立即从印刷材料的感兴趣的低聚物和/或聚合物。
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公开(公告)号：US06508923B1
公开(公告)日：2003-01-21
申请号：US09566976
申请日：2000-05-09
IPC分类号： C02F140
CPC分类号： G01N27/44782 , G01N27/44721
摘要： A data processing part comprises a time-series data production part for producing time-series data as to each capillary column from a scan waveform obtained by an optical measuring part, further comprises a correction data storage part for storing correction data indicating the relation between the number of data points of saturated parts and light intensity data obtained on the assumption that a peak is unsaturated as to a saturated scan waveform peak and a saturated data correction part obtaining light intensity data as to the saturated peak included in the scan waveform on the basis of the correction data stored in the correction data storage part, and employs the light intensity data obtained by the saturated data correction part as the time-series data.
摘要翻译： 一种数据处理部分包括一个时间序列数据生成部分，用于根据由光学测量部分获得的扫描波形来产生关于每个毛细管柱的时间序列数据，还包括校正数据存储部分，用于存储指示 基于峰值对饱和扫描波形峰值不饱和而获得的饱和部分的数据点数和光强度数据的数量以及饱和数据校正部分获得关于扫描波形中包括的饱和峰值的光强度数据 存储在校正数据存储部分中的校正数据，并且采用由饱和数据校正部分获得的光强度数据作为时间序列数据。
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公开(公告)号：US06461492B1
公开(公告)日：2002-10-08
申请号：US09443719
申请日：1999-11-19
IPC分类号： C25F702
CPC分类号： G01N21/645 , G01N21/6428 , G01N27/44721
摘要： A multi-capillary electrophoresis apparatus arranged with a fixed sample injection holder opposite a detection side holder within the same plane and an epi-optical detection system. A sample is injected sequentially and separated components are successively fed to the detection part for analysis by fluorescence. The epi-optical system adjusts the parallelism between the detection side holder and scanning axis of the detector. Thus, a capillary electrophoretic apparatus can detect fluorescence from a fluorochrome bonded to samples as a label without influence by Raman scattering or Rayleigh scattering.
摘要翻译： 一种多毛细管电泳装置，其具有与同一平面内的检测侧支架相对的固定样品注射保持器和外延光学检测系统。 依次注入样品，并将分离的组分依次送入检测部分进行荧光分析。 外延光学系统调节检测器侧检测器与检测器的扫描轴之间的平行度。 因此，毛细管电泳装置可以在不受拉曼散射或瑞利散射影响的情况下检测到作为标签结合到样品的荧光染料的荧光。
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公开(公告)号：US06458556B1
公开(公告)日：2002-10-01
申请号：US08899393
申请日：1997-07-23
IPC分类号： C12N900
CPC分类号： C12N9/1276 , C12N9/1252 , C12N9/22 , C12N9/96
摘要： A method for enhancing activity of enzyme at an elevated temperature which comprises adding a substance exhibiting chaperone function such as a saccharide to a reaction mixture containing the enzyme. The method can improve activity of enzymes more easily and more effectively and hence afford increased enzyme activity at an elevated temperature.
摘要翻译： 一种在升高的温度下增强酶的活性的方法，其包括在含有该酶的反应混合物中加入显示伴侣功能的物质如糖。 该方法可以更容易且更有效地提高酶的活性，从而在升高的温度下提高酶的活性。
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公开(公告)号：US06265569B1
公开(公告)日：2001-07-24
申请号：US09263520
申请日：1999-03-05
IPC分类号： C07H1900
CPC分类号： C07H19/04
摘要： Disclosed are 3′-deoxyribonucleotide derivatives represented by the following general formula [I]: Q—V—(CH2)n—NH—R [I] wherein Q represents a 3′-deoxyribonucleotide residue, n represents an integer not less than 4, V represents —C≡C— or —CH═CH—, and R represents a fluorescent group. The above 3′-deoxyribonucleotide derivatives are derivatives with improved rates for incorporation using RNA polymerases, which are useful as terminators in the DNA sequence determination methods utilizing RNA polymerases.
摘要翻译： 公开了由以下通式[I]表示的3'-脱氧核糖核苷酸衍生物：其中Q表示3'-脱氧核糖核苷酸残基，n表示不小于4的整数，V表示-C = C-或-CH = CH-， R代表荧光基团。上述3'-脱氧核糖核苷酸衍生物是使用RNA聚合酶掺入率提高的衍生物，其可用作使用RNA聚合酶的DNA序列测定方法中的终止子。
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