公开(公告)号：US08097414B2

公开(公告)日：2012-01-17

申请号：US12094896

申请日：2006-11-21

申请人： Yoshihide Hayashizaki ,  Yasumasa Mitani ,  Yuki Kawai

发明人： Yoshihide Hayashizaki ,  Yasumasa Mitani ,  Yuki Kawai

IPC分类号： C12P19/34

CPC分类号： C12Q1/6844 ,  C12Q1/6865 ,  C12Q2527/125 ,  C12Q2525/301

摘要： Problem to be solved There is provided a method for detecting and/or amplifying a nucleic acid contained in a biological sample such as blood or cells conveniently, rapidly, and effectively.Solution There is provided a method for detecting a nucleic acid contained in a sample, comprising the step of adding at least one substance selected from the group consisting of polyphenols, polyhydric alcohols, sugar acids, sugar alcohols, and hydrophilic biodegradable polymers to a sample, the step of complementarily binding an oligonucleotide complementary to a part of the nucleic acid sequence of a nucleic acid to be detected to a part of the nucleic acid sequence, and the step of detecting the nucleic acid to be detected.

摘要翻译： 待解决的问题提供一种方便，快速，有效地检测和/或扩增生物样品如血液或细胞中的核酸的方法。 溶液提供了一种检测样品中所含的核酸的方法，其包括将至少一种选自多酚，多元醇，糖酸，糖醇和亲水性可生物降解聚合物的物质加入到样品中的步骤， 将与要检测的核酸的一部分核酸序列互补的寡核苷酸互补结合到核酸序列的一部分的步骤和检测待检测的核酸的步骤。

公开(公告)号：US20090042197A1

公开(公告)日：2009-02-12

申请号：US12094896

申请日：2006-11-21

申请人： Yoshihide Hayashizaki ,  Yasumasa Mitani ,  Yuki Kawai

发明人： Yoshihide Hayashizaki ,  Yasumasa Mitani ,  Yuki Kawai

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6844 ,  C12Q1/6865 ,  C12Q2527/125 ,  C12Q2525/301

摘要： Problem to be Solved There is provided a method for detecting and/or amplifying a nucleic acid contained in a biological sample such as blood or cells conveniently, rapidly, and effectively.Solution There is provided a method for detecting a nucleic acid contained in a sample, comprising the step of adding at least one substance selected from the group consisting of polyphenols, polyhydric alcohols, sugar acids, sugar alcohols, and hydrophilic biodegradable polymers to a sample, the step of complementarily binding an oligonucleotide complementary to a part of the nucleic acid sequence of a nucleic acid to be detected to a part of the nucleic acid sequence, and the step of detecting the nucleic acid to be detected.

摘要翻译： 待解决的问题提供了一种方便，快速，有效地检测和/或扩增生物样品如血液或细胞中的核酸的方法。 溶液提供了一种检测样品中所含的核酸的方法，其包括将至少一种选自多酚，多元醇，糖酸，糖醇和亲水性可生物降解聚合物的物质加入到样品中的步骤， 将与要检测的核酸的一部分核酸序列互补的寡核苷酸互补结合到核酸序列的一部分的步骤和检测待检测的核酸的步骤。

公开(公告)号：US09518901B2

公开(公告)日：2016-12-13

申请号：US13807605

申请日：2012-06-28

申请人： Yoshihide Hayashizaki ,  Kengo Usui ,  Saori Goda ,  Kazuhito Nomura ,  Yuki Kawai

发明人： Yoshihide Hayashizaki ,  Kengo Usui ,  Saori Goda ,  Kazuhito Nomura ,  Yuki Kawai

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12P19/00 ,  C12P19/26 ,  C12P19/28 ,  C12P19/30 ,  G01N1/34 ,  C12N15/10

CPC分类号： G01N1/34 ,  C12N15/1003 ,  C12Q1/6806

摘要： The present invention is to provide a pretreatment method that allows RNA to be detected promptly and simply. RNA degradation activity due to lactoferrin present in the human rhinal mucosa is inhibited, for example, by adding iron ion and carbonate ion to a biological sample that contains the human rhinal mucosa. With the pretreated biological sample, an RNA virus gene can be amplified by a reverse transcriptase. Iron ion and carbonate ion can also inhibit reverse transcriptase inhibition due to lysozyme C contained in the human rhinal mucosa. Further, it is preferable to remove the envelope of the RNA virus by adding SDS to the biological sample that contains the human rhinal mucosa.

摘要翻译： 本发明提供一种能够及时且简单地检测RNA的预处理方法。 例如，通过将铁离子和碳酸根离子加入到含有人类鼻粘膜的生物样品中，可抑制由于存在于人类鼻粘膜中的乳铁蛋白引起的RNA降解活性。 通过预处理的生物样品，RNA病毒基因可以通过逆转录酶扩增。 铁离子和碳酸根离子也可以抑制人类鼻粘膜中包含的溶菌酶C的逆转录酶抑制。 此外，优选通过向包含人类鼻粘膜的生物样品中加入SDS来除去RNA病毒的包膜。

公开(公告)号：US09586987B2

公开(公告)日：2017-03-07

申请号：US14343511

申请日：2012-09-07

申请人： Yoshihide Hayashizaki ,  Yasumasa Kimura ,  Kengo Usui ,  Yuki Tanaka ,  Yuki Kawai

发明人： Yoshihide Hayashizaki ,  Yasumasa Kimura ,  Kengo Usui ,  Yuki Tanaka ,  Yuki Kawai

IPC分类号： C12P19/34 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C07H21/04 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q2525/155 ,  C12Q2525/301 ,  C12Q2531/119 ,  C12Q2531/125 ,  C12Q2525/161 ,  C12Q2527/101

摘要： The present invention provides a primer set including primers that can be designed easily, with which an amplification distance can be shortened. Provided is a primer set for use in a method for isothermally amplifying a target nucleic acid sequence 4. The primer set includes a first primer 1F and a second primer 1R. The first primer 1F includes, on the 3′ side thereof, a sequence (A′) that can hybridize to a sequence (A) on the 3′ side of the target nucleic acid sequence. The second primer 1R includes, on the 3′ side thereof, a sequence (B′) that can hybridize to a sequence (B) on the 3′ side of either a strand extended from the first primer or a complementary strand of the target nucleic acid sequence 4. The first primer 1F and the second primer 1R include, on the 5′ sides thereof, sequences (C) that are substantially identical to each other.

摘要翻译： 本发明提供了可以容易地设计的引物，其可以缩短放大距离。 提供了用于等温扩增靶核酸序列4的方法中的引物组。引物组包括第一引物1F和第二引物1R。 第一引物1F在其3'侧包含可以与靶核酸序列的3'侧的序列（A）杂交的序列（A'）。 第二引物1R在其3'侧具有可以与从第一引物延伸的链的3'侧的序列（B）或靶核酸的互补链上的序列（B'）杂交的序列 酸序列4.第一引物1F和第二引物1R在其5'侧包括彼此基本相同的序列（C）。

公开(公告)号：US20140295447A1

公开(公告)日：2014-10-02

申请号：US14343511

申请日：2012-09-07

申请人： Yoshihide Hayashizaki ,  Yasumasa Kimura ,  Kengo Usui ,  Yuki Tanaka ,  Yuki Kawai

发明人： Yoshihide Hayashizaki ,  Yasumasa Kimura ,  Kengo Usui ,  Yuki Tanaka ,  Yuki Kawai

IPC分类号： C07H21/04 ,  C12Q1/68

CPC分类号： C07H21/04 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q2525/155 ,  C12Q2525/301 ,  C12Q2531/119 ,  C12Q2531/125 ,  C12Q2525/161 ,  C12Q2527/101

摘要： The present invention provides a primer set including primers that can be designed easily, with which an amplification distance can be shortened. Provided is a primer set for use in a method for isothermally amplifying a target nucleic acid sequence 4. The primer set includes a first primer 1F and a second primer 1R. The first primer 1F includes, on the 3′ side thereof, a sequence (A′) that can hybridize to a sequence (A) on the 3′ side of the target nucleic acid sequence. The second primer 1R includes, on the 3′ side thereof, a sequence (B′) that can hybridize to a sequence (B) on the 3′ side of either a strand extended from the first primer or a complementary strand of the target nucleic acid sequence 4. The first primer 1F and the second primer 1R include, on the 5′ sides thereof, sequences (C) that are substantially identical to each other.

摘要翻译： 本发明提供了可以容易地设计的引物，其可以缩短放大距离。 提供了用于等温扩增靶核酸序列4的方法中的引物组。引物组包括第一引物1F和第二引物1R。 第一引物1F在其3'侧包含可以与靶核酸序列的3'侧的序列（A）杂交的序列（A'）。 第二引物1R在其3'侧具有可以与从第一引物延伸的链的3'侧的序列（B）或靶核酸的互补链上的序列（B'）杂交的序列 酸序列4.第一引物1F和第二引物1R在其5'侧包括彼此基本相同的序列（C）。

公开(公告)号：US20130302783A1

公开(公告)日：2013-11-14

申请号：US13807605

申请日：2012-06-28

申请人： Yoshihide Hayashizaki ,  Kengo Usui ,  Saori Goda ,  Kazuhito Nomura ,  Yuki Kawai

发明人： Yoshihide Hayashizaki ,  Kengo Usui ,  Saori Goda ,  Kazuhito Nomura ,  Yuki Kawai

IPC分类号： G01N1/34

CPC分类号： G01N1/34 ,  C12N15/1003 ,  C12Q1/6806

摘要： The present invention is to provide a pretreatment method that allows RNA to be detected promptly and simply. RNA degradation activity due to lactoferrin present in the human rhinal mucosa is inhibited, for example, by adding iron ion and carbonate ion to a biological sample that contains the human rhinal mucosa. With the pretreated biological sample, an RNA virus gene can be amplified by a reverse transcriptase. Iron ion and carbonate ion can also inhibit reverse transcriptase inhibition due to lysozyme C contained in the human rhinal mucosa. Further, it is preferable to remove the envelope of the RNA virus by adding SDS to the biological sample that contains the human rhinal mucosa.

摘要翻译： 本发明提供一种能够及时且简单地检测RNA的预处理方法。 例如，通过将铁离子和碳酸根离子加入到含有人类鼻粘膜的生物样品中，可抑制由于存在于人类鼻粘膜中的乳铁蛋白引起的RNA降解活性。 通过预处理的生物样品，RNA病毒基因可以通过逆转录酶扩增。 铁离子和碳酸根离子也可以抑制人类鼻粘膜中包含的溶菌酶C的逆转录酶抑制。 此外，优选通过向包含人类鼻粘膜的生物样品中加入SDS来除去RNA病毒的包膜。

公开(公告)号：US09023510B2

公开(公告)日：2015-05-05

申请号：US13635288

申请日：2011-03-11

申请人： Kyotaro Goto ,  Naoto Hagiwara ,  Yuki Kawai ,  Katsuei Ishida

发明人： Kyotaro Goto ,  Naoto Hagiwara ,  Yuki Kawai ,  Katsuei Ishida

IPC分类号： H01M2/02 ,  H01G11/22 ,  H01G11/08 ,  H01G11/78 ,  H01M2/06 ,  H01M2/10 ,  H01M10/04 ,  H01G11/82 ,  H01M2/22 ,  H01M10/0525 ,  H01M10/0583 ,  H01M10/42

CPC分类号： H01M2/0277 ,  H01G11/08 ,  H01G11/22 ,  H01G11/78 ,  H01G11/82 ,  H01M2/021 ,  H01M2/0212 ,  H01M2/06 ,  H01M2/1094 ,  H01M2/22 ,  H01M10/0436 ,  H01M10/045 ,  H01M10/0525 ,  H01M10/0583 ,  H01M10/425 ,  Y02E60/13

摘要： [Object] To provide an electrochemical device that permits a thin package, as well as securely prevents an electrolyte or gas in an internal space from leaking outside even when temperature rise occurs in the electrochemical device during the process where the electrochemical device is reflow soldered to a circuit board or encapsulated into an IC card.[Solution] In the electrochemical device RB1, the main body of the package PA includes first cover plate 15, first terminal plate 12, frame plate 14, second terminal plate 13, and second cover plate 16 stacked in this order and bonded at the facing surfaces. The electric storage element SD is encapsulated in internal space IS formed, between the cover plates 15, 16, by the through holes 12a1, 13a1 in the frame sections 12a, 13a of the terminal plates 12, 13 and the through hole 14a in the frame plate 14.

摘要翻译： 本发明提供允许薄封装的电化学装置，并且即使在电化学装置回流焊接到电化学装置期间在电化学装置中发生温度上升时，也可以可靠地防止内部空间中的电解质或气体泄漏到外部 电路板或封装在IC卡中。 [解决方案]在电化学装置RB1中，封装PA的主体包括依次层叠的第一盖板15，第一端子板12，框架板14，第二端子板13和第二盖板16， 表面。 蓄电元件SD被封装在通过端子板12,13的框架部分12a，13a中的通孔12a1，13a1和框架中的通孔14a之间形成在盖板15,16之间的内部空间IS中 板14。

公开(公告)号：US20130183575A1

公开(公告)日：2013-07-18

申请号：US13810887

申请日：2011-06-24

申请人： Kyotaro Goto ,  Yuki Kawai

发明人： Kyotaro Goto ,  Yuki Kawai

IPC分类号： H01M2/02 ,  H01G2/10

CPC分类号： H01M2/0202 ,  H01G2/10 ,  H01G11/80 ,  H01G11/82 ,  H01L2224/32225 ,  H01M2/0212 ,  H01M2/0217 ,  H01M2/0426 ,  H01M2/0473 ,  H01M2/10 ,  H01M2/30 ,  Y02E60/13

摘要： An electrochemical device which can keep a conductive lid properly joined to a rigid case is provided. A conductive lid 12 has a reinforcement part RP inside of a ring-shaped welded part PP. The welded part PP of the conductive lid 12 and the welded frame member 11d of a rigid case 11 are joined to each other by welding. Even if stress of risen internal pressure due to reflow soldering or the like concentrates at the interface between the rigid case 11 and the conductive lid 12, the reinforcement part RP relieves the stress.

摘要翻译： 提供一种能够将导电盖保持正确连接到刚性壳体的电化学装置。 导电盖12在环形焊接部PP内部具有加强部RP。 导电盖12的焊接部分PP和刚性壳体11的焊接框架部件11d通过焊接彼此接合。 即使由于回流焊等引起的内部压力上升的压力集中在刚性壳体11与导电盖12之间的界面处，则加强部分RP减轻了应力。

公开(公告)号：US20130108917A1

公开(公告)日：2013-05-02

申请号：US13574533

申请日：2011-01-11

申请人： Yuki Kawai ,  Katsuei Ishida

发明人： Yuki Kawai ,  Katsuei Ishida

IPC分类号： H01M2/06

CPC分类号： H01M2/06 ,  H01G9/016 ,  H01G9/155 ,  H01G11/74 ,  H01G11/80 ,  H01M2/021 ,  H01M2/08 ,  Y02E60/13

摘要： An electrochemical device free of any void connecting the inside and outside of a film package upon reflow soldering may be provided. Electrochemical device RB1 according to one embodiment comprises film package 14 having terminal-sealing sections 14c; electric storage element 11; seal auxiliary members 13; terminals 12 each electrically connected to the electric storage element 11 at one end and led out of the film package 14 via the corresponding terminal-sealing section 14c at the other end. The film package 14 is provided with support member 14d on its front edge. Provided on a part of the terminals 12 are heat seal auxiliary members 13, the rear ends of which are integrated with the terminal-sealing section 14c by thermal fusion bonding. The other end of the heat seal auxiliary member 13 projects outwardly from the terminal-sealing section 14c so as to be disposed on the support member 14d.

摘要翻译： 可以提供一种电化学装置，其不具有在回流焊接时连接膜封装的内部和外部的任何空隙。 根据一个实施例的电化学装置RB1包括具有端子密封部分14c的膜封装14; 蓄电元件11; 密封辅助构件13; 端子12在一端电连接到蓄电元件11，并在另一端通过相应的端子密封部分14c从薄膜封装14引出。 薄膜包装14在其前边缘上设置有支撑构件14d。 在端子12的一部分上设有热封辅助构件13，其后端通过热熔接而与端子密封部14c集成。 热封辅助构件13的另一端从端子密封部分14c向外突出，以便设置在支撑构件14d上。

公开(公告)号：US20130202951A1

公开(公告)日：2013-08-08

申请号：US13810889

申请日：2011-06-24

申请人： Yuki Kawai ,  Kyotaro Goto

发明人： Yuki Kawai ,  Kyotaro Goto

IPC分类号： H01G11/02 ,  H01G11/04 ,  H01G11/06 ,  H01M2/04

CPC分类号： H01G11/02 ,  H01G11/04 ,  H01G11/06 ,  H01G11/78 ,  H01G11/82 ,  H01M2/0212 ,  H01M2/0217 ,  H01M2/0287 ,  H01M2/0426 ,  H01M2/0473 ,  H01M2/0486 ,  H01M2/0491 ,  H01M2/06 ,  H01M2/26 ,  H01M2/30 ,  Y02E60/13

摘要： An electrochemical device which is free from deterioration of properties due to a process of joining a rigid case and a conductive lid is provided. A welded part PP of a conductive lid 12 and a welded frame member 11d are joined each other by laser welding. A weld bead for joining the welded part PP and the welded frame member 11d is not exposed to an internal space of a rigid package 10; therefore, fused material, the coagulum thereof, or the like created during laser welding are prevented from entering into the internal space of the rigid package 10.

摘要翻译： 提供了一种电化学装置，其不会由于刚性壳体和导电盖子的接合过程而导致的性能劣化。 导电盖12的焊接部分PP和焊接框架部件11d通过激光焊接彼此接合。 用于接合焊接部分PP和焊接框架部件11d的焊道不暴露于刚性包装10的内部空间; 因此，防止在激光焊接期间产生的熔融材料，其凝固物等进入刚性封装10的内部空间。