公开(公告)号：US20240167063A1

公开(公告)日：2024-05-23

申请号：US18144378

申请日：2023-05-08

申请人： LYGOS, INC.

发明人： Jeffrey DIETRICH ,  Mario OUELLET ,  Andrew Jonathan CONLEY

IPC分类号： C12P7/52

CPC分类号： C12P7/52 ,  C12Y101/01086 ,  C12Y102/01005 ,  C12Y202/01006 ,  C12Y401/01072 ,  C12Y402/01009

摘要： Methods and materials related to producing isobutyric acid are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, methods and materials for producing isobutyric by direct microbial fermentation from a carbon source are disclosed.

公开(公告)号：US11920177B2

公开(公告)日：2024-03-05

申请号：US17658217

申请日：2022-04-06

申请人： Sasya Inc.

发明人： Goutham Vemuri ,  Christopher Lindsay ,  Kevin Roberg-Perez ,  Christopher D. Snow ,  Elizabeth A. Cameron

IPC分类号： C12P7/16 ,  C12N1/20 ,  C12N9/04 ,  C12N9/88 ,  C12N15/52

CPC分类号： C12P7/16 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/01086

摘要： The biological production of beta-hydroxyisovalerate (βHIV) using a non-natural microorganism. The non-natural microorganism for the biologically-derived βHIV provides more beta-hydroxyisovalerate synthase activity than the wild-type parent. The non-natural microorganism can host a non-natural enzyme, such as the non-natural enzyme expressed in a yeast or bacteria, wherein the non-natural microorganism comprises an active βHIV metabolic pathway for the production of βHIV. The biological derivation of βHIV eliminates toxic by-products and impurities that result from the chemical production of βHIV, such that βHIV produced by a non-natural microorganism prior to any isolation or purification process has not been in substantial contact with any halogen-containing component.

公开(公告)号：US20170349918A1

公开(公告)日：2017-12-07

申请号：US15536417

申请日：2014-12-16

申请人： NEWPEK S.A. DE C.V.

发明人： Claudio Garibay ORIJEL ,  Carlos Eduardo Gomez SANCHEZ ,  Monica Maria Rios LOZANO ,  Jessica Valeria Guerrero TORRES ,  Sergio Rossano BECERRIL ,  Alejandra Cecilia Herrera RAMIREZ ,  Paola Rocha RUIZ ,  Anne-Laure Patricia CHAUVIN ,  Jesus Agustin Badillo CORONA ,  Ivan Alejandro de la Pena MIRELES ,  Jose Raul Ivan Garza RODRIGUEZ

IPC分类号： C12P7/16 ,  C12P7/24

CPC分类号： C12P7/16 ,  C12P7/24 ,  C12P7/40 ,  C12Y101/01001 ,  C12Y101/01027 ,  C12Y101/01028 ,  C12Y101/01086 ,  C12Y202/01006 ,  C12Y401/01072 ,  C12Y402/01009 ,  Y02E50/10

摘要： The present invention relates to a process of producing isobutanol, including: mixing water, lactate, an enzyme mixture including at least one enzyme, at least one cofactor, and at least one coenzyme, to prepare a reaction mixture; allowing catalytic conversions of lactate in the reaction mixture for a sufficient amount of time to produce isobutanol; and separating the isobutanol from a reactant obtained by the catalytic conversions, in which the conversion of lactate into isobutanol is in association with a NADH+/NADH and/or NADP+/NADPH regenerating system.

公开(公告)号：US20170226497A1

公开(公告)日：2017-08-10

申请号：US15406876

申请日：2017-01-16

申请人： Butamax Advanced Biofuels LLC

发明人： Kristen J. Kelly ,  Rick W. YE

IPC分类号： C12N9/88 ,  B01D11/04 ,  C12P7/16

CPC分类号： C12N9/88 ,  B01D11/04 ,  C12N15/52 ,  C12P7/16 ,  C12P7/40 ,  C12Y101/01001 ,  C12Y101/01086 ,  C12Y202/01006 ,  C12Y402/01009 ,  Y02E50/10 ,  Y02P20/52

摘要： Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.

公开(公告)号：US09689004B2

公开(公告)日：2017-06-27

申请号：US13833369

申请日：2013-03-15

申请人： BUTAMAX(TM) ADVANCED BIOFUELS LLC

发明人： Lori Ann Maggio-Hall

IPC分类号： C12P7/16 ,  C12N1/20

CPC分类号： C12P7/16 ,  C12N1/20 ,  C12Y101/01008 ,  C12Y101/01086 ,  C12Y102/01005 ,  C12Y401/01001 ,  C12Y402/01009 ,  Y02E50/10

摘要： The invention relates to the fields of industrial microbiology and alcohol production. More specifically, the invention relates to improved production of butanol isomers by recombinant microorganisms containing an engineered butanol pathway and disrupted activity of the genes in pathways for the production of by-products during the fermentation when the microorganisms are grown in a fermentation medium containing acetate. In embodiments, recombinant microorganisms have an increased growth rate in a fermentation medium containing acetate as a C2 supplement.

公开(公告)号：US20160319307A1

公开(公告)日：2016-11-03

申请号：US15209841

申请日：2016-07-14

申请人： BUTAMAX ADVANCED BIOFUELS LLC

发明人： Vasantha Nagarajan ,  Brian James Paul ,  Wonchul Suh ,  Jean-Francois Tomb ,  Rick W. Ye

IPC分类号： C12P7/16 ,  C12N9/88 ,  C12N15/52 ,  C12N9/04

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/88 ,  C12N15/52 ,  C12P7/42 ,  C12Y101/01008 ,  C12Y101/01086 ,  C12Y401/01001 ,  C12Y401/01072 ,  C12Y401/02009 ,  C12Y402/01009 ,  Y02E50/10 ,  Y02P20/52

摘要： Ketol-acid reductoisomerase enzymes have been identified that provide high effectiveness in vivo as a step in an isobutanol biosynthetic pathway in bacteria and in yeast. These KARIs are members of a clade identified through molecular phylogenetic analysis called the SLSL Clade.

摘要翻译： 已经鉴定了酮醇酸还原异构酶，其在细菌和酵母中作为异丁醇生物合成途径的步骤在体内提供高效力。 这些KARI是通过称为SLSL分子的分子系统发育分析鉴定的进化枝的成员。

公开(公告)号：US20160208294A1

公开(公告)日：2016-07-21

申请号：US14915099

申请日：2014-08-29

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Shota ATSUMI ,  Gabriel RODRIGUEZ ,  Yohei TASHIRO

IPC分类号： C12P7/62 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88

CPC分类号： C12P7/62 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1022 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/01001 ,  C12Y101/01086 ,  C12Y102/0101 ,  C12Y102/01025 ,  C12Y202/01006 ,  C12Y203/01084 ,  C12Y401/01074 ,  C12Y402/01009

摘要： The present disclosure provides recombinant bacteria with elevated 2-keto acid decarboxylase and alcohol transferase activities. Some recombinant bacteria further have elevated aldehyde dehydrogenase activity. Some recombinant bacteria further have reduced alcohol dehydrogenase and/or aldehyde reductase activity. Methods for the production of the recombinant bacteria, as well as for use thereof for production of various esters are also provided.

摘要翻译： 本公开提供了具有升高的2-酮酸脱羧酶和醇转移酶活性的重组细菌。 一些重组细菌还具有升高的醛脱氢酶活性。 一些重组细菌还具有还原的醇脱氢酶和/或醛还原酶活性。 还提供了生产重组细菌的方法以及用于生产各种酯的方法。

公开(公告)号：US09388392B2

公开(公告)日：2016-07-12

申请号：US14853108

申请日：2015-09-14

申请人： Butamax Advanced Biofuels LLC

发明人： Sridhar Govindarajan ,  Yougen Li ,  Der-Ing Liao ,  Daniel P. O'Keefe ,  Jeremy Stephen Minshull ,  Steven Cary Rothman ,  Alexander Vincent Tobias

IPC分类号： C12N9/04 ,  C12P13/06 ,  C12P7/16 ,  C12P7/44 ,  C12P7/42 ,  C12P13/02 ,  C12P13/08

CPC分类号： C12N9/0006 ,  C12P7/16 ,  C12P7/42 ,  C12P7/44 ,  C12P13/02 ,  C12P13/06 ,  C12P13/08 ,  C12Y101/01086 ,  Y02E50/10 ,  Y02P20/52

摘要： Provided herein are polypeptides having ketol-acid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways.

摘要翻译： 本文提供具有酮酸酸还原异构酶活性的多肽以及包含这些多肽的微生物宿主细胞。 本文提供的多肽可用于生物合成途径，包括但不限于异丁醇生物合成途径。

公开(公告)号：US09297029B2

公开(公告)日：2016-03-29

申请号：US14585261

申请日：2014-12-30

申请人： BUTAMAX ADVANCED BIOFUELS LLC

发明人： Gail K. Donaldson ,  Andrew C. Eliot ,  Dennis Flint ,  Lori Ann Maggio-Hall ,  Vasantha Nagarajan

IPC分类号： C12P7/16 ,  C12N15/70 ,  C12N15/81 ,  C12N15/74 ,  C12N9/04 ,  C12N9/02 ,  C12N9/06 ,  C12N9/10 ,  C12N9/88 ,  C12N15/52 ,  C12N15/75

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0016 ,  C12N9/1022 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12N15/70 ,  C12N15/74 ,  C12N15/746 ,  C12N15/75 ,  C12N15/81 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y101/01086 ,  C12Y102/00 ,  C12Y102/01003 ,  C12Y102/04004 ,  C12Y202/01006 ,  C12Y401/01072 ,  C12Y402/01009 ,  Y02E50/10 ,  Y02E50/343 ,  Y02P20/52

摘要： Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.

公开(公告)号：US09290770B2

公开(公告)日：2016-03-22

申请号：US14238527

申请日：2012-08-21

申请人： Hideaki Yukawa ,  Masayuki Inui

发明人： Hideaki Yukawa ,  Masayuki Inui

IPC分类号： C12N15/77 ,  C12P13/08 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10

CPC分类号： C12N15/77 ,  C12N9/0006 ,  C12N9/0016 ,  C12N9/1022 ,  C12P13/08 ,  C12Y101/01086 ,  C12Y104/01009 ,  C12Y202/01006 ,  Y02P20/52

摘要： A transformant obtainable by introducing one or more of the following DNAs (a), (b), and (c) into a coryneform bacterium as a host.(a) A DNA which encodes acetohydroxy acid synthase derived from Corynebacterium glutamicum and which has a mutation changing the glycine at position 156 to glutamic acid (G156E) in an amino acid sequence encoded by the DNA, or an analog thereof.(b) A DNA which encodes acetohydroxy acid isomeroreductase derived from Corynebacterium glutamicum and which has mutations changing the serine at position 34 to glycine (S34G), the leucine at position 48 to glutamic acid (L48E), and the arginine at position 49 to phenylalanine (R49F) in an amino acid sequence encoded by the DNA, or an analog thereof. (c) A DNA which encodes leucine dehydrogenase derived from Lysinibacillus sphaericus, or an analog thereof.

摘要翻译： 将一种或多种以下DNA（a），（b）和（c）引入作为宿主的棒状细菌获得的转化体。 （a）编码源自谷氨酸棒杆菌的乙酰羟酸合酶的DNA，其在DNA编码的氨基酸序列或其类似物中具有将位置156处的甘氨酸变为谷氨酸（G156E）的突变。 （b）编码源自谷氨酸棒杆菌的乙酰羟酸异构体还原酶的DNA，其具有将第34位的丝氨酸改变为甘氨酸（S34G），将第48位的亮氨酸变为谷氨酸（L48E），将第49位的精氨酸变为苯丙氨酸 （R49F），其由DNA编码的氨基酸序列或其类似物。 （c）编码源自嗜裂杆菌属的亮氨酸脱氢酶的DNA，或其类似物。